##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933878.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	524754
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35962184185352	33.0	31.0	34.0	31.0	34.0
2	32.58360107783838	34.0	31.0	34.0	31.0	34.0
3	32.653033611940074	34.0	31.0	34.0	31.0	34.0
4	36.03652568632159	37.0	35.0	37.0	35.0	37.0
5	36.05867511252892	37.0	35.0	37.0	35.0	37.0
6	35.67475426580836	37.0	35.0	37.0	35.0	37.0
7	36.01141487249264	37.0	35.0	37.0	35.0	37.0
8	36.00949016110406	37.0	35.0	37.0	35.0	37.0
9	37.86503009028993	39.0	38.0	39.0	35.0	39.0
10	37.57314665538519	39.0	37.0	39.0	35.0	39.0
11	37.498854701441054	39.0	37.0	39.0	35.0	39.0
12	37.10010023744459	39.0	35.0	39.0	34.0	39.0
13	36.87290997305404	39.0	35.0	39.0	33.0	39.0
14	37.730770989835236	40.0	36.0	41.0	33.0	41.0
15	37.92591766808828	40.0	36.0	41.0	33.0	41.0
16	38.12907571928942	40.0	36.0	41.0	33.0	41.0
17	38.14829424835256	40.0	36.0	41.0	34.0	41.0
18	38.13258402985018	40.0	36.0	41.0	34.0	41.0
19	38.163316525457645	40.0	36.0	41.0	34.0	41.0
20	38.045272642037986	40.0	36.0	41.0	34.0	41.0
21	37.96548096822511	40.0	36.0	41.0	34.0	41.0
22	38.004445892742126	40.0	36.0	41.0	34.0	41.0
23	37.99367132027579	40.0	36.0	41.0	34.0	41.0
24	37.94792035887292	40.0	35.0	41.0	34.0	41.0
25	37.81152692499724	40.0	35.0	41.0	34.0	41.0
26	37.76882501133865	40.0	35.0	41.0	34.0	41.0
27	37.741859995350204	40.0	35.0	41.0	34.0	41.0
28	37.66992152513368	40.0	35.0	41.0	33.0	41.0
29	37.65647331892658	40.0	35.0	41.0	33.0	41.0
30	37.603980150699186	40.0	35.0	41.0	33.0	41.0
31	37.43649024114156	39.0	35.0	41.0	33.0	41.0
32	37.36071949904146	39.0	35.0	41.0	33.0	41.0
33	37.34708072735034	39.0	35.0	41.0	33.0	41.0
34	37.27324613056785	39.0	35.0	41.0	33.0	41.0
35	37.1934982868163	39.0	35.0	41.0	32.0	41.0
36	37.102539475640015	39.0	35.0	41.0	32.0	41.0
37	37.00456023203253	39.0	35.0	41.0	32.0	41.0
38	36.93492379286294	39.0	35.0	41.0	32.0	41.0
39	36.938626480217394	39.0	35.0	41.0	32.0	41.0
40	36.78175297377438	39.0	35.0	41.0	32.0	41.0
41	36.62011151892125	39.0	35.0	41.0	31.0	41.0
42	36.61004013309093	39.0	35.0	41.0	31.0	41.0
43	36.52769678744707	38.0	35.0	41.0	31.0	41.0
44	36.4225789608083	38.0	35.0	40.0	31.0	41.0
45	36.440366343086474	38.0	35.0	40.0	31.0	41.0
46	36.374966174626586	38.0	35.0	40.0	31.0	41.0
47	36.25305381188138	38.0	35.0	40.0	31.0	41.0
48	36.20487123490245	38.0	35.0	40.0	31.0	41.0
49	36.215748712730154	38.0	35.0	40.0	31.0	41.0
50	36.126649820677876	38.0	35.0	40.0	31.0	41.0
51	35.120433574589235	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	9.0
11	13.0
12	10.0
13	6.0
14	13.0
15	23.0
16	27.0
17	47.0
18	92.0
19	177.0
20	307.0
21	520.0
22	763.0
23	1139.0
24	1474.0
25	2187.0
26	3000.0
27	3716.0
28	4520.0
29	5616.0
30	7084.0
31	9401.0
32	13087.0
33	19977.0
34	41180.0
35	47647.0
36	39024.0
37	59827.0
38	108862.0
39	154919.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.18589662965885	4.495058636999432	54.11583332380506	16.203211409536657
2	20.171165917744315	5.5250650781127915	51.94853207407662	22.355236930066276
3	21.783921608982496	5.811294435106736	49.95979068287236	22.444993273038413
4	20.499510246706077	4.8676141582532	50.191137180469326	24.441738414571397
5	18.700381512099	5.909054528407596	49.17351749581709	26.217046463676315
6	20.9532466641512	7.417570899888329	52.82418047313598	18.805001962824484
7	81.4686500722243	2.4903097451377216	10.812494997656044	5.228545184981916
8	82.07121813268694	3.878769861687572	8.246340189879449	5.8036718157460445
9	76.08860532744866	5.481425582272836	11.291004928023417	7.138964162255075
10	43.626537387042305	23.58552769488179	18.35259950376748	14.43533541430842
11	34.591446658815364	21.32237200669266	24.473562850402285	19.61261848408969
12	30.345457109426512	19.05826349108344	29.94393563460212	20.652343764887927
13	27.13404757276743	21.811934735133036	31.702473921113512	19.351543770986023
14	21.289213612473652	24.352363202567297	33.444051879547374	20.914371305411677
15	18.10181532680075	21.911028786822015	38.200566360618495	21.786589525758735
16	23.42087911669087	21.617557941435415	31.338874977608555	23.62268796426516
17	24.019254736505104	22.537989229238846	27.509080445313423	25.933675588942627
18	24.88956730201199	21.6173673759514	31.166413214572923	22.326652107463687
19	25.853828651139395	22.43260651657729	28.605594240348807	23.107970591934507
20	25.21810219645777	27.306509335803064	28.462670127335855	19.01271834040331
21	25.15654954512019	25.200189040960147	30.28771576776928	19.35554564615039
22	22.65366247803733	22.24337499094814	31.03244567930878	24.07051685170575
23	21.290357005377757	23.401441437321107	30.31744398327597	24.990757574025164
24	23.559991920023478	23.24841735365524	28.63151114617516	24.560079580146127
25	23.28595875400664	24.388380079046563	26.782644820239575	25.54301634670722
26	21.90245334004124	24.604671903406167	26.6791677624182	26.813706994134396
27	21.796880061895667	23.555990044859115	29.884860334556762	24.762269558688452
28	19.39727948715017	23.81001383505414	30.00739394077987	26.785312737015822
29	22.506355358891977	24.321110463188468	27.89630950883652	25.276224669083035
30	25.565693639305277	22.252712699664986	27.245718946401553	24.935874714628188
31	24.287380372517408	24.796952476779595	27.85629075719289	23.059376393510103
32	27.18931156313244	25.500710809255384	24.780182714186076	22.5297949134261
33	26.27364441242944	22.030322779816828	26.601798175907188	25.09423463184654
34	22.169054452181403	22.824218586232785	30.404722974955884	24.602003986629924
35	22.42345937334446	25.19980790999211	26.43390998448797	25.942822732175458
36	25.829055138217143	24.169229772426696	26.057352588069836	23.944362501286317
37	22.109216890199978	26.52042671423181	28.26600654783003	23.104349847738177
38	23.38352828182348	26.774831635394868	25.551972924456027	24.289667158325614
39	23.60668046360771	22.572481581845967	28.547471767723543	25.273366186822777
40	24.654981191186728	21.883396791639512	28.620458348102158	24.8411636690716
41	20.33105035883481	23.21335330459606	27.96796213082702	28.48763420574212
42	21.397454807395466	22.894346684351145	29.726881548306444	25.98131695994695
43	22.58696455863128	21.71036333215183	30.04569760306734	25.65697450614955
44	22.833365729465616	21.67053514599222	28.309646043669982	27.18645308087218
45	22.452425326915087	21.73742363088228	25.722529032651494	30.08762200955114
46	23.907583362870984	21.645380502101936	28.40130804148229	26.045728093544785
47	19.478460383341528	23.304634171440334	32.14992167758607	25.066983767632074
48	20.199179043894855	24.256127633138576	29.53631606428917	26.008377258677402
49	23.332266166622837	20.783071686923776	31.1540264581118	24.730635688341586
50	21.743521726370833	21.61508059014319	29.076290985871474	27.5651066976145
51	20.464446197646897	22.78496209652523	25.687083852624276	31.063507853203596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	266.5
2	436.0
3	1046.0
4	1656.0
5	1154.5
6	653.0
7	670.5
8	688.0
9	787.0
10	886.0
11	891.5
12	897.0
13	869.5
14	842.0
15	872.5
16	903.0
17	821.5
18	740.0
19	794.0
20	848.0
21	980.0
22	1112.0
23	1283.5
24	1455.0
25	1815.0
26	2369.5
27	2564.0
28	3027.5
29	3491.0
30	4425.5
31	5360.0
32	6380.0
33	7400.0
34	8160.5
35	8921.0
36	10202.5
37	11484.0
38	13108.0
39	14732.0
40	17301.0
41	19870.0
42	22947.5
43	26025.0
44	29569.5
45	33114.0
46	40942.0
47	48770.0
48	55927.0
49	63084.0
50	62057.5
51	61031.0
52	52064.0
53	43097.0
54	38915.0
55	34733.0
56	32138.0
57	29543.0
58	27259.0
59	24975.0
60	22545.0
61	20115.0
62	18092.5
63	16070.0
64	13897.0
65	11724.0
66	10293.0
67	8862.0
68	7419.5
69	5977.0
70	4839.5
71	3702.0
72	3386.0
73	3070.0
74	2359.5
75	1406.0
76	1163.0
77	839.5
78	516.0
79	347.5
80	179.0
81	128.0
82	77.0
83	58.0
84	39.0
85	31.0
86	23.0
87	11.5
88	0.0
89	1.0
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	524754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.546609574597483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.59431172521558	21.258702688829917
2	12.497807821883526	7.635313121468531
3	4.960905309905702	4.546165129147109
4	2.5335800208879906	3.095691188962639
5	1.465349231497543	2.238072543249595
6	1.004127186176077	1.840360867161587
7	0.7015493108627234	1.500096702737607
8	0.5609347852525794	1.370772470553698
9	0.4729796462382457	1.3003132131333832
>10	5.847096157305906	42.67498028062291
>50	0.2919880045556785	5.532820620639627
>100	0.059911145643173784	3.121349970969809
>500	0.005045149106793581	1.152951896331192
>1k	0.004414505468444384	2.732409306192419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	3032	0.5777945475403713	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	2653	0.5055702290978249	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	2638	0.5027117468375658	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	2350	0.4478288874405912	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	1258	0.23973137889372925	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	1162	0.22143709242807108	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	1091	0.20790694306284468	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.18351456110863376	No Hit
TCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	926	0.17646363819999467	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	765	0.14578259527321374	No Hit
GCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	763	0.1454014643051792	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	740	0.1410184581727819	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	692	0.1318713149399528	No Hit
TGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	577	0.10995628427796643	No Hit
GTGGAAGGAAAAGAGCAGATCTTGGATGTGTTTCCACTTTGGGCCTCAACC	559	0.10652610556565552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9056548401727287E-4	0.0	0.0	0.5286286526639149	0.0
2	1.9056548401727287E-4	0.0	0.0	2.189406845874448	0.0
3	1.9056548401727287E-4	0.0	0.0	3.1096475681938585	0.0
4	1.9056548401727287E-4	0.0	0.0	4.026267546316941	0.0
5	1.9056548401727287E-4	0.0	0.0	5.734687110531792	0.0
6	1.9056548401727287E-4	0.0	0.0	6.703140900307573	0.0
7	1.9056548401727287E-4	0.0	0.0	7.620332574882707	0.0
8	1.9056548401727287E-4	0.0	0.0	8.979445606893897	0.0
9	1.9056548401727287E-4	0.0	0.0	9.641279532885886	0.0
10	1.9056548401727287E-4	0.0	0.0	11.036028310408305	0.0
11	1.9056548401727287E-4	0.0	0.0	13.283367063424004	0.0
12	1.9056548401727287E-4	0.0	0.0	15.557575549686138	0.0
13	1.9056548401727287E-4	0.0	0.0	16.22893774987899	0.0
14	1.9056548401727287E-4	0.0	0.0	16.498206778795396	0.0
15	1.9056548401727287E-4	0.0	0.0	16.902396170396035	0.0
16	1.9056548401727287E-4	0.0	0.0	17.726210757802704	0.0
17	1.9056548401727287E-4	0.0	0.0	18.810528361860985	0.0
18	1.9056548401727287E-4	0.0	0.0	20.00156263696894	0.0
19	1.9056548401727287E-4	0.0	0.0	20.872828029895913	0.0
20	1.9056548401727287E-4	0.0	0.0	21.784493305434548	0.0
21	1.9056548401727287E-4	0.0	0.0	22.97781436635071	0.0
22	1.9056548401727287E-4	0.0	0.0	24.12387518723059	0.0
23	3.8113096803454573E-4	0.0	0.0	25.21867389290982	0.0
24	3.8113096803454573E-4	0.0	0.0	26.04306017676854	0.0
25	3.8113096803454573E-4	0.0	0.0	26.799795713801135	0.0
26	3.8113096803454573E-4	0.0	0.0	27.468489997217745	0.0
27	3.8113096803454573E-4	0.0	0.0	28.23246702264299	0.0
28	5.716964520518185E-4	0.0	0.0	28.903638657351827	0.0
29	5.716964520518185E-4	0.0	0.0	29.677334522461955	0.0
30	5.716964520518185E-4	0.0	0.0	30.60767521543428	0.0
31	5.716964520518185E-4	0.0	0.0	31.364791883434904	0.0
32	5.716964520518185E-4	0.0	0.0	32.02090884490637	0.0
33	5.716964520518185E-4	0.0	0.0	32.668831490565104	0.0
34	5.716964520518185E-4	0.0	0.0	33.42023119404521	0.0
35	5.716964520518185E-4	0.0	0.0	34.17563277268968	0.0
36	5.716964520518185E-4	0.0	0.0	34.85137797901493	0.0
37	5.716964520518185E-4	0.0	0.0	35.57895699699288	0.0
38	5.716964520518185E-4	0.0	0.0	36.351128338230865	0.0
39	5.716964520518185E-4	0.0	0.0	37.838110810017646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	20	7.0297765E-4	45.000004	44
GGTACGT	20	7.0297765E-4	45.000004	7
CTTCACG	20	7.0297765E-4	45.000004	1
ATTACGC	20	7.0297765E-4	45.000004	40
CATTACG	20	7.0297765E-4	45.000004	39
TGCATCA	40	6.8030204E-9	45.000004	35
GTTACGG	30	2.1632386E-6	45.000004	2
CCGTAGT	30	2.1632386E-6	45.000004	29
CTTATAG	20	7.0297765E-4	45.000004	21
CTAGTGA	20	7.0297765E-4	45.000004	35
TTAAGCG	20	7.0297765E-4	45.000004	24
CGTAGAT	20	7.0297765E-4	45.000004	22
TACGAAA	30	2.1632386E-6	45.000004	20
CCATATA	20	7.0297765E-4	45.000004	15
CATACGA	30	2.1632386E-6	45.000004	18
TCCGTAG	30	2.1632386E-6	45.000004	28
TAGGATC	20	7.0297765E-4	45.000004	19
CACGTGG	20	7.0297765E-4	45.000004	13
GTACGTC	20	7.0297765E-4	45.000004	8
ATCGACT	20	7.0297765E-4	45.000004	21
>>END_MODULE