FastQCFastQC Report
Sat 14 Jan 2017
SRR2933877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537881
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90850.5907479187271316No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC75820.49301603960254403No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC75700.49223574515843554No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG66110.4298772141667659No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC40210.2614636633133513TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC38660.25138486007695005TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT36660.23837995267514198Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC26970.17537117631338187TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG26570.17277019483302025No Hit
GAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT17570.11424811152488393No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT16350.106315118009781No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTC16330.10618506893576292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA207.034031E-445.044
CGTTTTT41550.042.4007231
CGAATAT1000.040.514
TTTCGCG4750.039.7894741
TACGGGA3450.039.1304324
CCGATGA4700.038.77659618
TACGAAT1050.038.5714312
GACCGAT5400.038.3333369
TTTGGGC29900.037.851174
TACGGCT10000.037.5749977
TTGGGAC32350.037.279755
TTTGGGA84200.036.823044
AATCGTT552.750312E-936.8181822
TTGGGAT45150.036.777415
TTACGGG3550.036.7605633
GCCGATT1600.036.56259
GGGACCG8100.036.388897
TTGGGCA16200.036.388895
TCTTGCG4050.036.111111
TCGCATA250.002107329436.020
Produced by FastQC (version 0.11.5)