##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933877.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1537881
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4677871694884	33.0	31.0	34.0	31.0	34.0
2	32.69477677401567	34.0	31.0	34.0	31.0	34.0
3	32.738947942005915	34.0	31.0	34.0	31.0	34.0
4	36.12464813597411	37.0	35.0	37.0	35.0	37.0
5	36.145424125793866	37.0	35.0	37.0	35.0	37.0
6	35.72049462864812	37.0	35.0	37.0	35.0	37.0
7	36.0479809556136	37.0	35.0	37.0	35.0	37.0
8	36.02833899371928	37.0	36.0	37.0	35.0	37.0
9	37.90636336621624	39.0	38.0	39.0	35.0	39.0
10	37.64791228970252	39.0	37.0	39.0	35.0	39.0
11	37.58258018663343	39.0	37.0	39.0	35.0	39.0
12	37.39287240040029	39.0	37.0	39.0	35.0	39.0
13	37.326603943998265	39.0	37.0	39.0	35.0	39.0
14	38.48239818295434	40.0	38.0	41.0	34.0	41.0
15	38.56031838614301	40.0	38.0	41.0	34.0	41.0
16	38.66435049265841	40.0	38.0	41.0	35.0	41.0
17	38.610363220561275	40.0	38.0	41.0	35.0	41.0
18	38.53974397238798	40.0	38.0	41.0	35.0	41.0
19	38.51816818076301	40.0	37.0	41.0	34.0	41.0
20	38.3941156695479	40.0	37.0	41.0	34.0	41.0
21	38.35047900325188	40.0	37.0	41.0	34.0	41.0
22	38.361247066580574	40.0	37.0	41.0	34.0	41.0
23	38.298623235477905	40.0	37.0	41.0	34.0	41.0
24	38.270266685133635	40.0	37.0	41.0	34.0	41.0
25	38.200719691575614	40.0	37.0	41.0	34.0	41.0
26	38.1360293806868	40.0	37.0	41.0	34.0	41.0
27	38.115250789885565	40.0	37.0	41.0	34.0	41.0
28	38.03059534515349	40.0	36.0	41.0	34.0	41.0
29	38.00624950825194	40.0	36.0	41.0	34.0	41.0
30	37.926610056304746	40.0	36.0	41.0	34.0	41.0
31	37.84136744000349	40.0	36.0	41.0	34.0	41.0
32	37.67719283871769	40.0	36.0	41.0	33.0	41.0
33	37.57247992529981	40.0	36.0	41.0	33.0	41.0
34	37.45711859370133	40.0	36.0	41.0	33.0	41.0
35	37.35030538773806	40.0	36.0	41.0	33.0	41.0
36	37.26543341129776	40.0	35.0	41.0	33.0	41.0
37	37.18337959829142	40.0	35.0	41.0	32.0	41.0
38	37.15813642277914	40.0	35.0	41.0	32.0	41.0
39	37.09365549089949	40.0	35.0	41.0	32.0	41.0
40	36.981173445799776	39.0	35.0	41.0	32.0	41.0
41	36.82864408884693	39.0	35.0	41.0	31.0	41.0
42	36.785329294009095	39.0	35.0	41.0	31.0	41.0
43	36.72764472673764	39.0	35.0	41.0	31.0	41.0
44	36.65672311446725	39.0	35.0	41.0	31.0	41.0
45	36.666290174597385	39.0	35.0	41.0	31.0	41.0
46	36.59189170033312	39.0	35.0	41.0	31.0	41.0
47	36.48670735902193	39.0	35.0	41.0	31.0	41.0
48	36.43456873451197	39.0	35.0	41.0	31.0	41.0
49	36.43691742078873	39.0	35.0	41.0	31.0	41.0
50	36.359856191733954	39.0	35.0	41.0	31.0	41.0
51	35.47291695521305	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	19.0
10	32.0
11	24.0
12	20.0
13	12.0
14	26.0
15	31.0
16	71.0
17	128.0
18	224.0
19	454.0
20	893.0
21	1576.0
22	2508.0
23	3671.0
24	5504.0
25	8060.0
26	11259.0
27	13028.0
28	14141.0
29	15360.0
30	18222.0
31	22971.0
32	31152.0
33	46118.0
34	90952.0
35	107634.0
36	106345.0
37	169003.0
38	323012.0
39	545119.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.535850953357247	4.421408418466708	52.90233769713001	17.14040293104603
2	20.471870060167205	6.846303452607841	51.113447659474296	21.56837882775065
3	20.32634514634097	7.064915946032235	50.70580883696463	21.902930070662162
4	18.669910090572678	4.783334991459027	52.37479362837567	24.171961289592627
5	20.460880913412677	5.783997591491149	49.25329072925668	24.50183076583949
6	20.99778851549632	8.098741059938968	52.286945478876454	18.616524945688255
7	81.14821627941303	2.3501818411177458	12.130587477184514	4.371014402284702
8	82.39064010804476	4.6235046794908055	8.842231616100335	4.143623596364088
9	76.88592290300745	5.0513661330102915	10.794463290722755	7.268247673259505
10	39.59669181165513	28.47782110579427	17.845724083983093	14.07976299856751
11	29.591626400222125	22.759303223071225	27.650318847817225	19.99875152888943
12	25.8699470245097	20.597367416594654	31.75343215762468	21.77925340127097
13	25.010907866083265	20.704137706363497	33.89755124096078	20.387403186592458
14	21.080889873793875	23.596819259747665	33.33983578703424	21.98245507942422
15	18.750150369241833	23.148345027996314	36.277579344565666	21.82392525819618
16	23.455260842678985	23.87284841935104	30.214561464768728	22.457329273201243
17	23.746635793016495	24.56347402692406	29.604046086790852	22.085844093268594
18	23.5546183352288	23.325211768660903	30.930936788997325	22.18923310711297
19	23.59499857271141	24.780330857849208	29.58739980531654	22.03727076412284
20	24.41515305800644	26.931212493034245	29.738646878399567	18.914987570559752
21	25.18816475396991	25.901548949496096	30.164817693956813	18.745468602577184
22	23.793388435125994	22.001247170619834	31.7305435205975	22.474820873656675
23	22.61416845646705	24.264231107608456	30.857394037640102	22.264206398284394
24	22.754816530017603	24.224371066421917	29.93469585748182	23.086116546078664
25	22.472805113009393	26.500489959886366	28.09346106753383	22.93324385957041
26	21.13479521497437	25.618627188969757	28.771471914927098	24.475105681128774
27	21.39476331393651	25.298251295126217	30.300263804546645	23.006721586390626
28	18.762700104884576	25.644571979236364	30.582795417850928	25.00993249802813
29	20.48032324997838	24.838137671250244	30.375042022107042	24.306497056664334
30	21.615131469860152	25.635793666740142	29.999200198194785	22.749874665204914
31	22.128630238620545	25.357943820100516	27.91041699585339	24.603008945425557
32	23.220327190465323	26.669813854257907	26.522013081636352	23.58784587364042
33	22.612282744893786	24.74294174906901	26.612722310763964	26.032053195273235
34	20.44560014721555	24.70795854815815	29.15076003930083	25.695681265325472
35	19.85205617339703	27.133828950354417	28.258168219777737	24.75594665647082
36	20.934909788208582	27.12277477906288	26.944802621269137	24.997512811459405
37	20.135758228367475	28.33073560307982	27.11841813508327	24.415088033469427
38	20.6523781749043	26.89115737823668	26.11983632023544	26.33662812662358
39	20.75063025032496	25.717789607908543	26.52454903857971	27.007031103186783
40	22.362523498242062	24.15011304515759	27.826925490333775	25.66043796626657
41	19.692876106798902	25.706280264857945	27.00547051429857	27.595373114044584
42	19.890615723843393	25.339866998812	29.03768236944211	25.731834907902496
43	20.936080229874744	24.07474960676411	28.67523560015372	26.313934563207425
44	21.479815408344336	23.74962692171891	28.291135660041316	26.47942200989543
45	20.668829382767587	23.315588137183564	27.670866601512078	28.344715878536764
46	21.554138454145672	24.43121411864767	28.3068065734605	25.707840853746163
47	18.457214830016106	24.848346523560664	31.266398375426967	25.42804027099626
48	19.392202647669098	25.535655879746223	29.309680007750927	25.762461464833752
49	20.879573907213885	23.62978670001125	30.26196435224832	25.228675040526543
50	20.319387520881005	23.61079953520461	28.760872915394625	27.308940028519764
51	19.64222199246886	23.444401744998476	27.617611505701678	29.295764756830987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	776.0
2	1338.0
3	7849.5
4	14361.0
5	9238.0
6	4115.0
7	3982.5
8	3850.0
9	4018.0
10	4186.0
11	4145.0
12	4104.0
13	3983.5
14	3863.0
15	3681.5
16	3500.0
17	3343.0
18	3186.0
19	3489.0
20	3792.0
21	4380.5
22	4969.0
23	5948.5
24	6928.0
25	7948.5
26	11195.5
27	13422.0
28	16199.0
29	18976.0
30	22291.5
31	25607.0
32	29198.0
33	32789.0
34	36591.0
35	40393.0
36	44705.5
37	49018.0
38	53459.5
39	57901.0
40	63093.0
41	68285.0
42	72770.0
43	77255.0
44	85816.0
45	94377.0
46	120562.5
47	146748.0
48	147950.0
49	149152.0
50	147235.0
51	145318.0
52	127639.5
53	109961.0
54	99327.5
55	88694.0
56	83653.5
57	78613.0
58	73555.5
59	68498.0
60	62848.0
61	57198.0
62	51163.5
63	45129.0
64	39654.5
65	34180.0
66	29345.5
67	24511.0
68	20696.5
69	16882.0
70	13762.0
71	10642.0
72	8812.0
73	6982.0
74	5861.0
75	3742.5
76	2745.0
77	2065.0
78	1385.0
79	1014.5
80	644.0
81	414.0
82	184.0
83	180.0
84	176.0
85	114.0
86	52.0
87	46.5
88	41.0
89	23.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1537881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.287255257815087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5586669578988	21.250442003627747
2	10.526945433757296	6.166106760070817
3	3.7039675559961425	3.254371298373736
4	1.858847747098263	2.1776219381872535
5	1.1535922513711339	1.689277536467199
6	0.8290193715026977	1.4567821168123762
7	0.6265482993744533	1.2844915982590692
8	0.5503413216461092	1.289438941277833
9	0.4777171606649382	1.2591921982889434
>10	7.487315339530741	49.941251431235095
>50	0.17446003120319978	3.180016049723867
>100	0.04628708296635866	2.5329722020389194
>500	0.0017975562828167308	0.3926037394015277
>1k	0.0035951125656334616	2.095422103590923
>5k	8.987781414083654E-4	2.030010082644708
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9085	0.5907479187271316	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	7582	0.49301603960254403	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	7570	0.49223574515843554	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	6611	0.4298772141667659	No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	4021	0.2614636633133513	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3866	0.25138486007695005	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	3666	0.23837995267514198	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	2697	0.17537117631338187	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	2657	0.17277019483302025	No Hit
GAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	1757	0.11424811152488393	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	1635	0.106315118009781	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTC	1633	0.10618506893576292	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9507361102712108E-4	0.0	0.0	0.24787353507846185	0.0
2	1.9507361102712108E-4	0.0	0.0	0.9819355333735185	0.0
3	1.9507361102712108E-4	0.0	0.0	1.5122106326822427	0.0
4	1.9507361102712108E-4	0.0	0.0	2.1328048138965237	0.0
5	1.9507361102712108E-4	0.0	0.0	3.5577525179126344	0.0
6	1.9507361102712108E-4	0.0	0.0	4.490139354085264	0.0
7	1.9507361102712108E-4	0.0	0.0	5.312049501879534	0.0
8	1.9507361102712108E-4	0.0	0.0	6.373379994941091	0.0
9	1.9507361102712108E-4	0.0	0.0	6.942734840992248	0.0
10	1.9507361102712108E-4	0.0	0.0	8.233601949695718	0.0
11	1.9507361102712108E-4	0.0	0.0	9.631889593538121	0.0
12	1.9507361102712108E-4	0.0	0.0	11.356925535850953	0.0
13	1.9507361102712108E-4	0.0	0.0	11.835701201848517	0.0
14	1.9507361102712108E-4	0.0	0.0	12.055939308698138	0.0
15	1.9507361102712108E-4	0.0	0.0	12.392701385867957	0.0
16	1.9507361102712108E-4	0.0	0.0	12.914003099069435	0.0
17	1.9507361102712108E-4	0.0	0.0	13.575757812210437	0.0
18	1.9507361102712108E-4	0.0	0.0	14.251947972567448	0.0
19	1.9507361102712108E-4	0.0	0.0	14.89289483386556	0.0
20	1.9507361102712108E-4	0.0	0.0	15.495477218328336	0.0
21	2.6009814803616146E-4	0.0	0.0	16.2655628101264	0.0017556624992440897
22	2.6009814803616146E-4	0.0	0.0	17.105484754672176	0.0017556624992440897
23	2.6009814803616146E-4	0.0	0.0	17.862175291846377	0.0017556624992440897
24	2.6009814803616146E-4	0.0	0.0	18.46911432028876	0.0017556624992440897
25	2.6009814803616146E-4	0.0	0.0	19.010118468203977	0.0017556624992440897
26	2.6009814803616146E-4	0.0	0.0	19.493250778181146	0.0017556624992440897
27	2.6009814803616146E-4	0.0	0.0	20.018649037214193	0.0017556624992440897
28	2.6009814803616146E-4	0.0	0.0	20.52493008236658	0.0017556624992440897
29	2.6009814803616146E-4	0.0	0.0	21.0853765668475	0.0017556624992440897
30	2.6009814803616146E-4	0.0	0.0	21.749797286005876	0.0017556624992440897
31	2.6009814803616146E-4	0.0	0.0	22.277796526519282	0.0017556624992440897
32	2.6009814803616146E-4	0.0	0.0	22.802804638330272	0.0017556624992440897
33	2.6009814803616146E-4	0.0	0.0	23.326317185790057	0.0017556624992440897
34	2.6009814803616146E-4	0.0	0.0	23.86335483694772	0.0017556624992440897
35	2.6009814803616146E-4	0.0	0.0	24.439732332995856	0.0017556624992440897
36	2.6009814803616146E-4	0.0	0.0	24.97286851193298	0.0017556624992440897
37	2.6009814803616146E-4	0.0	0.0	25.521155407993206	0.0017556624992440897
38	3.251226850452018E-4	0.0	0.0	26.126923994769427	0.0017556624992440897
39	3.251226850452018E-4	0.0	0.0	27.047151242521366	0.0017556624992440897
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTA	20	7.034031E-4	45.0	44
CGTTTTT	4155	0.0	42.400723	1
CGAATAT	100	0.0	40.5	14
TTTCGCG	475	0.0	39.789474	1
TACGGGA	345	0.0	39.130432	4
CCGATGA	470	0.0	38.776596	18
TACGAAT	105	0.0	38.57143	12
GACCGAT	540	0.0	38.333336	9
TTTGGGC	2990	0.0	37.85117	4
TACGGCT	1000	0.0	37.574997	7
TTGGGAC	3235	0.0	37.27975	5
TTTGGGA	8420	0.0	36.82304	4
AATCGTT	55	2.750312E-9	36.81818	22
TTGGGAT	4515	0.0	36.77741	5
TTACGGG	355	0.0	36.760563	3
GCCGATT	160	0.0	36.5625	9
GGGACCG	810	0.0	36.38889	7
TTGGGCA	1620	0.0	36.38889	5
TCTTGCG	405	0.0	36.11111	1
TCGCATA	25	0.0021073294	36.0	20
>>END_MODULE