##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933876.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1149996
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41219186849346	33.0	31.0	34.0	31.0	34.0
2	32.6358674291041	34.0	31.0	34.0	31.0	34.0
3	32.69833808117593	34.0	31.0	34.0	31.0	34.0
4	36.07014980921673	37.0	35.0	37.0	35.0	37.0
5	36.09775773133124	37.0	35.0	37.0	35.0	37.0
6	35.69513807004546	37.0	35.0	37.0	35.0	37.0
7	36.030367931714544	37.0	35.0	37.0	35.0	37.0
8	36.057731505153065	37.0	36.0	37.0	35.0	37.0
9	37.88310220209462	39.0	38.0	39.0	35.0	39.0
10	37.58888900483132	39.0	37.0	39.0	35.0	39.0
11	37.5162183172811	39.0	37.0	39.0	35.0	39.0
12	37.18901022264425	39.0	37.0	39.0	34.0	39.0
13	37.01707484199945	39.0	37.0	39.0	33.0	39.0
14	37.862648217906845	40.0	37.0	41.0	33.0	41.0
15	38.02287486217343	40.0	37.0	41.0	33.0	41.0
16	38.22432773679213	40.0	37.0	41.0	33.0	41.0
17	38.27021311378474	40.0	37.0	41.0	34.0	41.0
18	38.2383330028974	40.0	37.0	41.0	34.0	41.0
19	38.25664263180046	40.0	37.0	41.0	34.0	41.0
20	38.16105447323295	40.0	37.0	41.0	34.0	41.0
21	38.08744552154964	40.0	36.0	41.0	34.0	41.0
22	38.15275444436328	40.0	36.0	41.0	34.0	41.0
23	38.12976566875015	40.0	36.0	41.0	34.0	41.0
24	38.100411653605754	40.0	36.0	41.0	34.0	41.0
25	37.994344328154185	40.0	36.0	41.0	34.0	41.0
26	37.94189979791234	40.0	36.0	41.0	34.0	41.0
27	37.93719282501852	40.0	36.0	41.0	34.0	41.0
28	37.85811341952494	40.0	36.0	41.0	34.0	41.0
29	37.816727188616305	40.0	36.0	41.0	34.0	41.0
30	37.72575122000424	40.0	35.0	41.0	33.0	41.0
31	37.62219868590847	40.0	35.0	41.0	33.0	41.0
32	37.54888364829095	40.0	35.0	41.0	33.0	41.0
33	37.475814698485905	40.0	35.0	41.0	33.0	41.0
34	37.365427358008205	40.0	35.0	41.0	32.0	41.0
35	37.318410672732774	40.0	35.0	41.0	32.0	41.0
36	37.236473865996054	40.0	35.0	41.0	32.0	41.0
37	37.17796757553939	39.0	35.0	41.0	32.0	41.0
38	37.134519598329035	39.0	35.0	41.0	32.0	41.0
39	37.08232637330912	39.0	35.0	41.0	32.0	41.0
40	36.95681289326224	39.0	35.0	41.0	32.0	41.0
41	36.80107322112425	39.0	35.0	41.0	31.0	41.0
42	36.79364971704249	39.0	35.0	41.0	31.0	41.0
43	36.73281907067503	39.0	35.0	41.0	31.0	41.0
44	36.66157273590517	39.0	35.0	41.0	31.0	41.0
45	36.67787627087399	39.0	35.0	41.0	31.0	41.0
46	36.633238724308605	39.0	35.0	41.0	31.0	41.0
47	36.56084455945934	39.0	35.0	41.0	31.0	41.0
48	36.47106946458944	38.0	35.0	41.0	31.0	41.0
49	36.465889446571985	38.0	35.0	40.0	31.0	41.0
50	36.38379090014226	38.0	35.0	40.0	31.0	41.0
51	35.418303194098065	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	13.0
10	26.0
11	28.0
12	19.0
13	14.0
14	16.0
15	36.0
16	87.0
17	138.0
18	226.0
19	407.0
20	661.0
21	1051.0
22	1602.0
23	2387.0
24	3301.0
25	4983.0
26	6763.0
27	8329.0
28	9747.0
29	11595.0
30	14904.0
31	19565.0
32	27543.0
33	43219.0
34	86759.0
35	85166.0
36	78753.0
37	125294.0
38	238263.0
39	378891.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.00365566488927	5.126017829627234	52.811574996782596	16.0587515087009
2	21.049986260821775	4.571146334422033	52.05392018754848	22.32494721720771
3	22.696513727004266	4.729668624934347	49.90599967304234	22.667817975019044
4	20.45311461952911	5.255583497681731	49.351302091485536	24.93999979130362
5	19.25650176174526	6.443674586694215	48.89477876444788	25.40504488711265
6	22.381295239287788	7.238633873509125	52.23600777741836	18.14406310978473
7	83.13820221983381	2.8892274407910987	9.054292362756044	4.9182779766190485
8	84.44733720812943	3.2992288668830154	7.520026156612719	4.733407768374846
9	78.34792468843371	6.134543076671571	9.939425876263916	5.578106358630812
10	40.847359469076416	28.201837223781652	17.031885328296795	13.918917978845144
11	33.70837811609779	23.43555977586009	24.648085732472115	18.207976375570002
12	30.73358516029621	20.848420342331625	28.305228887752655	20.11276560961951
13	28.689056309761078	21.319726329482886	30.5638454394624	19.42737192129364
14	22.30920803202794	23.221645988333872	33.9892486582562	20.479897321381987
15	20.571984598207298	23.107645591810755	35.94064675007565	20.379723059906297
16	26.405396192682407	23.177471921641466	29.976104264710486	20.441027620965638
17	26.942789366223884	23.26051568875022	27.550965394662242	22.245729550363652
18	26.984180814541965	22.454078101141224	29.8127993488673	20.748941735449513
19	26.548353211663343	24.86826041134056	27.58653073575908	20.996855641237016
20	25.295305374975218	28.545229722538167	27.910792733192114	18.2486721692945
21	25.231392109189944	25.399392693539802	31.30063061088908	18.06858458638117
22	23.15103704708538	22.83547073207211	32.570808941944144	21.44268327889836
23	22.108163854482974	24.268171367552583	31.826110699515475	21.797554078448968
24	23.09894990939099	25.36069690677185	29.168971022507904	22.371382161329258
25	24.681303239315618	25.434436293691455	26.127656096195118	23.756604370797813
26	20.82042024493998	24.969478154706625	27.110268209628558	27.099833390724836
27	20.85024643563978	24.188605873411735	30.47697557208895	24.484172118859544
28	19.277806183673682	25.30669671894511	28.77183920639724	26.643657890983967
29	21.80790194052849	26.767310494993023	27.23174689303267	24.193040671445814
30	24.670694506763503	23.82982201677223	28.19401110960386	23.305472366860407
31	24.38912831001151	24.781216630318713	28.989231266891363	21.84042379277841
32	26.53339663790135	25.29374015213966	25.829915930142366	22.342947279816624
33	25.852872531730547	25.06852197746775	26.213047697557208	22.8655577932445
34	21.576596788162743	24.661303169750155	30.896107464721617	22.86599257736549
35	21.028247054772365	27.74009648729213	27.21026855745585	24.021387900479656
36	23.61677779748799	27.552356703849405	25.310088035088818	23.520777463573786
37	22.57216546840163	28.635577862879526	26.326352439486744	22.465904229232102
38	24.674694520676592	27.570791550579305	25.512262651348355	22.24225127739575
39	22.68903544012327	25.93061193256324	26.69878851752528	24.681564109788205
40	23.71599553389751	24.814086309865427	28.628012619174324	22.841905537062736
41	20.249113909961423	25.803307141937886	27.160442297190603	26.787136650910092
42	20.65807185416297	25.47843644673547	28.751317395886595	25.112174303214967
43	21.18276933137159	24.1602579487233	29.072448947648518	25.584523772256603
44	23.075036782736635	23.183645856159497	27.661139690920667	26.080177670183204
45	21.728945144157024	22.828079402015312	26.52235312122825	28.920622332599415
46	21.152073572429817	23.66382143937892	28.514881790893188	26.669223197298074
47	18.959892034407076	25.170783202724184	31.36576127221312	24.50356349065562
48	20.248766082664634	27.05374627390008	28.596969032935764	24.100518610499517
49	23.423472777296617	24.050344522937472	29.52184181510197	23.004340884663947
50	21.347378599577738	24.917391017012232	27.637922218859888	26.097308164550135
51	19.919808416724926	25.470697289381878	26.360265600923828	28.249228692969368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	618.0
2	1054.0
3	2384.5
4	3715.0
5	2584.5
6	1454.0
7	1479.0
8	1504.0
9	1571.0
10	1638.0
11	1700.5
12	1763.0
13	1739.0
14	1715.0
15	1722.5
16	1730.0
17	1734.5
18	1739.0
19	1785.0
20	1831.0
21	2212.5
22	2594.0
23	3534.5
24	4475.0
25	5588.0
26	8244.5
27	9788.0
28	11326.0
29	12864.0
30	15469.5
31	18075.0
32	20558.5
33	23042.0
34	26907.0
35	30772.0
36	32711.5
37	34651.0
38	38083.5
39	41516.0
40	45653.0
41	49790.0
42	53707.0
43	57624.0
44	64550.0
45	71476.0
46	81541.0
47	91606.0
48	105218.0
49	118830.0
50	119435.0
51	120040.0
52	105397.5
53	90755.0
54	84756.0
55	78757.0
56	73216.5
57	67676.0
58	62630.5
59	57585.0
60	50567.5
61	43550.0
62	37887.5
63	32225.0
64	28321.5
65	24418.0
66	20212.5
67	16007.0
68	12797.5
69	9588.0
70	8197.5
71	6807.0
72	5730.5
73	4654.0
74	3699.0
75	2205.5
76	1667.0
77	1193.5
78	720.0
79	548.0
80	376.0
81	294.5
82	213.0
83	131.5
84	50.0
85	38.5
86	27.0
87	14.5
88	2.0
89	3.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1149996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.115098250647037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7390891856332	20.59575531800994
2	11.398062280516264	6.637114063284501
3	4.346725692159373	3.7966603678749564
4	2.195845578358428	2.55729039028618
5	1.3417347509705777	1.9532369550407904
6	0.931925217933348	1.6279856569431046
7	0.7043655624486207	1.4355370788544697
8	0.5594232976498209	1.3030131419820397
9	0.44412805382676135	1.1637748730731332
>10	7.0100094955552	48.70790104205018
>50	0.25819318699654165	4.66423973181968
>100	0.06567734253672743	3.473366326269181
>500	0.0027114499210376393	0.5656785554102127
>1k	0.002108905494140386	1.5184464991015958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	3267	0.28408794465372056	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	3027	0.26321830684628467	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	2960	0.2573921996250422	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	2559	0.22252251312178478	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.17573974170344941	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1777	0.15452227659922294	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1700	0.14782660113600393	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.695682419764938E-5	0.0	0.0	0.2771313987179086	0.0
2	8.695682419764938E-5	0.0	0.0	1.1015690489358223	0.0
3	8.695682419764938E-5	0.0	0.0	1.601136003951318	0.0
4	8.695682419764938E-5	0.0	0.0	2.171051029742712	0.0
5	8.695682419764938E-5	0.0	0.0	3.139576137656131	0.0
6	8.695682419764938E-5	0.0	0.0	3.8596655988368656	0.0
7	8.695682419764938E-5	0.0	0.0	4.506798284515773	0.0
8	8.695682419764938E-5	0.0	0.0	5.522801818441107	0.0
9	8.695682419764938E-5	0.0	0.0	6.043499281736632	0.0
10	8.695682419764938E-5	0.0	0.0	6.950893742239104	0.0
11	8.695682419764938E-5	0.0	0.0	8.583247246077377	0.0
12	8.695682419764938E-5	0.0	0.0	10.086730736454735	0.0
13	8.695682419764938E-5	0.0	0.0	10.596123812604565	0.0
14	8.695682419764938E-5	0.0	0.0	10.80682019763547	0.0
15	8.695682419764938E-5	0.0	0.0	11.108125593480326	0.0
16	8.695682419764938E-5	0.0	0.0	11.684910208383334	0.0
17	8.695682419764938E-5	0.0	0.0	12.467347712513783	0.0
18	8.695682419764938E-5	0.0	0.0	13.293959283336637	0.0
19	8.695682419764938E-5	0.0	0.0	13.900483132115243	0.0
20	1.7391364839529876E-4	0.0	0.0	14.626485657341417	0.0
21	1.7391364839529876E-4	0.0	0.0	15.587097694252849	0.0
22	2.6087047259294813E-4	0.0	0.0	16.628318707195504	0.0
23	2.6087047259294813E-4	0.0	0.0	17.561365430836283	0.0
24	2.6087047259294813E-4	0.0	0.0	18.236932998027818	0.0
25	2.6087047259294813E-4	0.0	0.0	18.867804757581766	0.0
26	2.6087047259294813E-4	0.0	0.0	19.421893641369188	0.0
27	2.6087047259294813E-4	0.0	0.0	20.035026208786814	0.0
28	2.6087047259294813E-4	0.0	0.0	20.616158664899704	0.0
29	2.6087047259294813E-4	0.0	0.0	21.29363928222359	0.0
30	2.6087047259294813E-4	0.0	0.0	22.14233788639265	0.0
31	2.6087047259294813E-4	0.0	0.0	22.767731366022144	0.0
32	2.6087047259294813E-4	0.0	0.0	23.381298717560757	0.0
33	3.478272967905975E-4	0.0	0.0	24.002779140101357	0.0
34	3.478272967905975E-4	0.0	0.0	24.686955432888464	0.0
35	3.478272967905975E-4	0.0	0.0	25.436871084768992	0.0
36	4.347841209882469E-4	0.0	0.0	26.05356888197872	0.0
37	4.347841209882469E-4	0.0	0.0	26.69617981279935	0.0
38	4.347841209882469E-4	0.0	0.0	27.55296540161879	0.0
39	4.347841209882469E-4	0.0	0.0	29.232971245117373	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTA	25	3.8908078E-5	45.0	8
CCGTACT	20	7.0332875E-4	45.0	33
CGTAGTA	20	7.0332875E-4	45.0	43
CGCGTAA	25	3.8908078E-5	45.0	13
GCTAACG	20	7.0332875E-4	45.0	9
CGATAAG	20	7.0332875E-4	45.0	24
TACCGCG	25	3.8908078E-5	45.0	1
CGAATAT	95	0.0	42.631577	14
AACCCGC	55	6.184564E-11	40.909092	21
TTTCTCG	340	0.0	39.705883	1
TATTGCG	40	3.4582263E-7	39.375	1
CGATAAC	40	3.4582263E-7	39.375	16
AGACACG	115	0.0	39.130436	24
CGGGCAA	190	0.0	39.078945	6
TACGAAT	110	0.0	38.863636	12
CGTTTTT	1170	0.0	38.653847	1
TTACGGG	175	0.0	38.57143	3
TGGGCGA	1225	0.0	38.571426	6
CCGTTGT	35	6.2482923E-6	38.571426	39
TTTCGCG	270	0.0	38.333332	1
>>END_MODULE