FastQCFastQC Report
Sat 14 Jan 2017
SRR2933875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573952
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138190.8779810311877363No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC60420.3838744764770463No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT52410.3329834709063555Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTG37750.2398421298743545No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC32390.20578772414914814No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC31440.19975196194038955No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT29790.18926879599886148No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC28710.18240708738258854No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT22160.14079209531167405No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT17670.11226517708290977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78350.043.7077261
CTATGCG501.0822987E-940.51
TTTCGCG3800.039.671051
TTATCCG403.4593177E-739.3751
CGCCGTA356.249762E-638.57142636
TACGGGA3100.038.467744
TTTGGGA93850.038.0234454
TTTGGGC32400.037.9166684
TATGCGG2150.037.674422
TCGTTTG5050.037.4257431
TTTTGCG13550.037.195571
GTTTTTT96550.037.053342
TTGGGAC35550.037.0253145
TTGGGAT55550.036.9396935
CGGGATC6750.036.6666686
ACACGCG3500.036.64285736
TGGGACG4000.036.56256
TTGGGAA40750.036.5521475
TGGGCGA18250.036.4931536
GTTGGGA29800.036.241614