##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933875.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1573952
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49161283190339	33.0	31.0	34.0	31.0	34.0
2	32.73904731529297	34.0	31.0	34.0	31.0	34.0
3	32.7391044961981	34.0	31.0	34.0	31.0	34.0
4	36.13679133798235	37.0	35.0	37.0	35.0	37.0
5	36.180763454031634	37.0	35.0	37.0	35.0	37.0
6	35.83568495100232	37.0	35.0	37.0	35.0	37.0
7	36.134544128410525	37.0	36.0	37.0	35.0	37.0
8	36.15322894217867	37.0	37.0	37.0	35.0	37.0
9	38.019488523156994	39.0	39.0	39.0	37.0	39.0
10	37.73186984101167	39.0	38.0	39.0	35.0	39.0
11	37.661382303907615	39.0	37.0	39.0	35.0	39.0
12	37.51910922315293	39.0	37.0	39.0	35.0	39.0
13	37.47690717378929	39.0	37.0	39.0	35.0	39.0
14	38.632722598910256	40.0	38.0	41.0	35.0	41.0
15	38.713381983694546	40.0	38.0	41.0	35.0	41.0
16	38.819493860041476	40.0	38.0	41.0	35.0	41.0
17	38.8059311846867	40.0	38.0	41.0	35.0	41.0
18	38.694471623022814	40.0	38.0	41.0	35.0	41.0
19	38.62980700809173	40.0	37.0	41.0	35.0	41.0
20	38.49863528239743	40.0	37.0	41.0	35.0	41.0
21	38.42438142967511	40.0	37.0	41.0	34.0	41.0
22	38.44832688671573	40.0	37.0	41.0	35.0	41.0
23	38.406317981742774	40.0	37.0	41.0	35.0	41.0
24	38.37808205078681	40.0	37.0	41.0	34.0	41.0
25	38.29930391778148	40.0	37.0	41.0	34.0	41.0
26	38.24418088988737	40.0	37.0	41.0	34.0	41.0
27	38.241243697393564	40.0	37.0	41.0	34.0	41.0
28	38.16738502826007	40.0	37.0	41.0	34.0	41.0
29	38.13711091570772	40.0	37.0	41.0	34.0	41.0
30	38.03940018501199	40.0	36.0	41.0	34.0	41.0
31	37.910468044768834	40.0	36.0	41.0	34.0	41.0
32	37.722337784125564	40.0	36.0	41.0	33.0	41.0
33	37.52617042959379	40.0	36.0	41.0	33.0	41.0
34	37.34111459561664	40.0	36.0	41.0	33.0	41.0
35	37.21505293681129	40.0	36.0	41.0	32.0	41.0
36	37.0779795063636	40.0	36.0	41.0	32.0	41.0
37	36.98823216972309	40.0	35.0	41.0	31.0	41.0
38	36.93237595555646	40.0	35.0	41.0	31.0	41.0
39	36.867057572276664	40.0	35.0	41.0	31.0	41.0
40	36.78286631358517	40.0	35.0	41.0	31.0	41.0
41	36.62520203919814	40.0	35.0	41.0	31.0	41.0
42	36.572799551701706	39.0	35.0	41.0	30.0	41.0
43	36.46482675456431	39.0	35.0	41.0	30.0	41.0
44	36.38722083011426	39.0	35.0	41.0	30.0	41.0
45	36.413660645305576	39.0	35.0	41.0	30.0	41.0
46	36.37026986845851	39.0	35.0	41.0	30.0	41.0
47	36.27455030394828	39.0	35.0	41.0	30.0	41.0
48	36.18667024153214	39.0	35.0	41.0	30.0	41.0
49	36.16457808116131	39.0	35.0	41.0	30.0	41.0
50	36.09434658744358	39.0	35.0	41.0	30.0	41.0
51	35.1552639470581	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	18.0
10	40.0
11	28.0
12	33.0
13	23.0
14	26.0
15	44.0
16	74.0
17	149.0
18	244.0
19	456.0
20	818.0
21	1396.0
22	2310.0
23	3617.0
24	6113.0
25	11034.0
26	16481.0
27	18661.0
28	18052.0
29	18228.0
30	19310.0
31	22838.0
32	29897.0
33	43588.0
34	83696.0
35	94984.0
36	100995.0
37	160874.0
38	323524.0
39	596018.0
40	375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.418155064449234	4.199492741837108	54.14186709632822	19.240485097385434
2	23.055849225389338	4.215948135648355	52.022615683324524	20.705586955637784
3	19.114877709104217	4.532603281421543	54.60096623022811	21.75155277924613
4	18.07977625747164	4.562909161143415	53.71682236815354	23.640492213231408
5	18.003217378928962	5.517067864839588	52.35572622290896	24.12398853332249
6	20.124120684747695	6.335580754686293	54.97963089090392	18.5606676696621
7	80.11921583377384	2.149493758386533	13.020092099377873	4.711198308461757
8	82.13541454885537	2.7299434798519906	10.863990769731224	4.27065120156142
9	76.45690592851625	5.024549668604887	13.027462082706462	5.491082320172406
10	36.026448074655384	30.191136705566624	20.113129244093848	13.669285975684137
11	26.27488004716789	23.344612796324157	31.477961208473953	18.902545948033993
12	23.940946102549507	21.471684015776844	33.998940247224816	20.588429634448826
13	23.457449782458422	20.890344813564834	35.83698867563941	19.815216728337333
14	19.432867075997233	23.289782661733014	36.10211747245151	21.175232789818242
15	17.53560464359777	24.51624954255276	37.50832299841419	20.439822815435285
16	21.979196316024886	24.218972370186638	33.323506688895215	20.47832462489326
17	22.37628593502216	24.596048672386452	31.659288212092875	21.368377180498516
18	22.234667893302973	23.884400540804293	33.21791261741146	20.663018948481277
19	22.20715752449884	25.929253242792665	31.631269568576425	20.23231966413207
20	22.648530577806696	27.475806123693737	31.172615175049813	18.70304812344976
21	22.767339791810677	25.88801945675599	32.99389053795795	18.35075021347538
22	22.04870288293417	23.00298865530842	33.40298814703371	21.5453203147237
23	20.347761558166958	24.417199507990077	33.03982586508356	22.195213068759404
24	20.653997072337656	25.203373419265645	32.10301203594519	22.03961747245151
25	21.378923880779084	25.965340747367136	30.277098666287156	22.378636705566624
26	20.366885394217867	25.85263082991095	31.059015776847072	22.72146799902411
27	20.34738035213272	24.83214227625747	32.89585705281991	21.9246203187899
28	19.107507725775626	24.463007766437606	33.160159903224496	23.269324604562275
29	20.085873012645873	23.93281370715244	33.08823903143171	22.893074248769977
30	22.535820660350506	23.17656446956451	32.693563717317936	21.594051152767047
31	22.93735768308055	24.384606392062782	31.873335400317167	20.804700524539506
32	23.48991582970764	24.508244215833773	31.24847517586305	20.753364778595536
33	23.624799231488637	23.79164040580653	30.51147684300411	22.072083519700726
34	21.196707396413615	23.917819603139105	32.58860498922458	22.296868011222706
35	22.017126316431504	24.847835258000245	31.19034125564185	21.944697169926403
36	23.783253873053308	25.20845616638881	29.78496167608669	21.22332828447119
37	22.580358232017243	26.6005570690847	30.54603952344163	20.27304517545643
38	22.053722095718296	25.439530557475702	31.02826515675192	21.47848219005408
39	21.476893831578092	24.866577888016916	29.95104043833611	23.70548784206888
40	24.33860753059814	23.581405277924613	30.044626519741392	22.03536067173586
41	22.968743646566097	24.7899554751352	28.888174480543245	23.353126397755457
42	21.684651120237465	25.489277944943684	30.345525149432767	22.480545785386084
43	21.413105355182367	24.697513011832637	31.595309132680033	22.294072500304964
44	21.722644654983124	23.776455698776076	30.75563930793315	23.74526033830765
45	21.26691284105233	22.629216138738663	30.20536839751149	25.898502622697517
46	22.052070202903266	23.12986673037043	30.837217399259952	23.98084566746635
47	20.368600821371935	24.158297076403855	32.794837453747	22.67826464847721
48	20.485885211238973	24.537470011791974	31.963808299109502	23.01283647785955
49	21.98453320050421	22.98418249095271	32.363185154312205	22.668099154230877
50	21.233430244378482	22.892311836701502	31.170327938844387	24.703929980075632
51	20.447764607815234	22.672800695319808	30.160894360183793	26.71854033668117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	872.0
2	1483.0
3	11894.0
4	22305.0
5	14813.5
6	7322.0
7	7298.5
8	7275.0
9	7459.0
10	7643.0
11	7408.0
12	7173.0
13	7003.5
14	6834.0
15	6567.0
16	6300.0
17	6111.0
18	5922.0
19	5995.0
20	6068.0
21	6310.0
22	6552.0
23	7140.0
24	7728.0
25	9358.5
26	13327.0
27	15665.0
28	18504.5
29	21344.0
30	24808.0
31	28272.0
32	31337.0
33	34402.0
34	40158.0
35	45914.0
36	50621.0
37	55328.0
38	59829.0
39	64330.0
40	69482.0
41	74634.0
42	81630.0
43	88626.0
44	97724.0
45	106822.0
46	126064.5
47	145307.0
48	157417.5
49	169528.0
50	165224.0
51	160920.0
52	136421.0
53	111922.0
54	98888.5
55	85855.0
56	76984.0
57	68113.0
58	61491.5
59	54870.0
60	48645.5
61	42421.0
62	36391.5
63	30362.0
64	26600.5
65	22839.0
66	19408.5
67	15978.0
68	13162.0
69	10346.0
70	8268.5
71	6191.0
72	5087.0
73	3983.0
74	3533.0
75	2293.0
76	1503.0
77	1204.0
78	905.0
79	671.5
80	438.0
81	272.0
82	106.0
83	72.0
84	38.0
85	39.5
86	41.0
87	21.0
88	1.0
89	3.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1573952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.15286819979122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91880978497962	24.174676900870757
2	10.646735909134415	7.059396647070354
3	3.8290371382377986	3.808306907283105
4	1.9428567351891153	2.576450930912057
5	1.2835970328618305	2.127746162605568
6	0.978069338497179	1.945548232167238
7	0.736079493456109	1.708220250178363
8	0.6417673997678514	1.7021144015541043
9	0.5207691757309846	1.5538492660970924
>10	6.308118535896096	43.66126592924832
>50	0.14305808662021688	3.05468371846216
>100	0.04587510058270516	2.774565754581017
>500	0.0025163517629150133	0.6184947651101329
>1k	0.0021292207224665495	1.6238214916317495
>5k	3.8713104044846357E-4	0.7240585634714286
>10k+	1.9356552022423178E-4	0.8868000787566845
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13819	0.8779810311877363	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	6042	0.3838744764770463	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	5241	0.3329834709063555	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTG	3775	0.2398421298743545	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	3239	0.20578772414914814	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	3144	0.19975196194038955	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT	2979	0.18926879599886148	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	2871	0.18240708738258854	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT	2216	0.14079209531167405	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	1767	0.11226517708290977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3456903387142683	0.0
2	0.0	0.0	0.0	1.1676976172081486	0.0
3	0.0	0.0	0.0	1.7889363843370065	0.0
4	0.0	0.0	0.0	2.408586792989875	0.0
5	0.0	0.0	0.0	3.588927743666897	0.0
6	0.0	0.0	0.0	4.805864473630708	0.0
7	0.0	0.0	0.0	5.780354165819542	0.0
8	0.0	0.0	0.0	7.0767723539218474	0.0
9	6.35343390395641E-5	0.0	0.0	7.9008127312649945	0.0
10	6.35343390395641E-5	0.0	0.0	9.071115256373766	0.0
11	6.35343390395641E-5	0.0	0.0	10.721356178587403	0.0
12	1.270686780791282E-4	0.0	0.0	12.158121721628106	0.0
13	1.270686780791282E-4	0.0	0.0	12.725292766234295	0.0
14	1.270686780791282E-4	0.0	0.0	13.005097995364535	0.0
15	1.270686780791282E-4	0.0	0.0	13.292527345179522	0.0
16	1.906030171186923E-4	0.0	0.0	13.812174704184118	0.0
17	1.906030171186923E-4	0.0	0.0	14.508955800431018	0.0
18	1.906030171186923E-4	0.0	0.0	15.263997885577197	0.0
19	1.906030171186923E-4	0.0	0.0	15.853088277152034	0.0
20	1.906030171186923E-4	0.0	0.0	16.460031817996992	0.0
21	1.906030171186923E-4	0.0	0.0	17.304784389867034	0.0
22	1.906030171186923E-4	0.0	0.0	18.215930346033424	0.0
23	1.906030171186923E-4	0.0	0.0	19.07351685438946	0.0
24	1.906030171186923E-4	0.0	0.0	19.722647196356686	0.0
25	1.906030171186923E-4	0.0	0.0	20.314215427154068	0.0
26	1.906030171186923E-4	0.0	0.0	20.83144848127516	0.0
27	1.906030171186923E-4	0.0	0.0	21.39976314398406	0.0
28	1.906030171186923E-4	0.0	0.0	21.956895763021997	0.0
29	1.906030171186923E-4	0.0	0.0	22.57737211808238	0.0
30	1.906030171186923E-4	0.0	0.0	23.289782661733014	0.0
31	1.906030171186923E-4	0.0	0.0	23.90403265156752	0.0
32	1.906030171186923E-4	0.0	0.0	24.48664254056032	0.0
33	1.906030171186923E-4	0.0	0.0	25.061120034156062	0.0
34	1.906030171186923E-4	0.0	0.0	25.64893973895011	0.0
35	1.906030171186923E-4	0.0	0.0	26.286062090838858	0.0
36	1.906030171186923E-4	0.0	0.0	26.86428811043793	0.0
37	1.906030171186923E-4	0.0	0.0	27.451281868824463	0.0
38	1.906030171186923E-4	0.0	0.0	28.10778219412028	0.0
39	1.906030171186923E-4	0.0	0.0	29.13487831903387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7835	0.0	43.707726	1
CTATGCG	50	1.0822987E-9	40.5	1
TTTCGCG	380	0.0	39.67105	1
TTATCCG	40	3.4593177E-7	39.375	1
CGCCGTA	35	6.249762E-6	38.571426	36
TACGGGA	310	0.0	38.46774	4
TTTGGGA	9385	0.0	38.023445	4
TTTGGGC	3240	0.0	37.916668	4
TATGCGG	215	0.0	37.67442	2
TCGTTTG	505	0.0	37.425743	1
TTTTGCG	1355	0.0	37.19557	1
GTTTTTT	9655	0.0	37.05334	2
TTGGGAC	3555	0.0	37.025314	5
TTGGGAT	5555	0.0	36.939693	5
CGGGATC	675	0.0	36.666668	6
ACACGCG	350	0.0	36.642857	36
TGGGACG	400	0.0	36.5625	6
TTGGGAA	4075	0.0	36.552147	5
TGGGCGA	1825	0.0	36.493153	6
GTTGGGA	2980	0.0	36.24161	4
>>END_MODULE