SRR2933874.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933874.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432918
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	2808	0.648621678932269	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2729	0.6303734194466388	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2102	0.48554229669360016	TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1998	0.4615192715479606	TruSeq Adapter, Index 16 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	1038	0.23976827020359515	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	971	0.22429189823476964	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	842	0.19449410742912052	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	756	0.1746289135586878	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	727	0.16793018539307675	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	666	0.15383975718265352	TruSeq Adapter, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	626	0.1446001321266383	TruSeq Adapter, Index 27 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	491	0.11341639756258691	TruSeq Adapter, Index 27 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	35	1.2095734E-7	45.000004	5
GTAGCGG	35	1.2095734E-7	45.000004	2
TTTAGCG	25	3.8867627E-5	45.0	1
TCGTTCG	45	3.8380676E-10	45.0	1
GGTACAT	20	7.0284103E-4	45.0	8
GGTCGCA	20	7.0284103E-4	45.0	23
TTAGCGA	20	7.0284103E-4	45.0	29
GCAACGA	20	7.0284103E-4	45.0	13
CAACCCG	20	7.0284103E-4	45.0	23
TTCCGTA	20	7.0284103E-4	45.0	1
AGGAACG	20	7.0284103E-4	45.0	10
CGTTCTA	20	7.0284103E-4	45.0	29
AAACGGC	20	7.0284103E-4	45.0	16
TGACCGT	20	7.0284103E-4	45.0	16
GGAACGA	25	3.8867627E-5	45.0	8
TCGTCGT	20	7.0284103E-4	45.0	31
CTGCAAC	20	7.0284103E-4	45.0	27
AACGAGG	20	7.0284103E-4	45.0	10
GTATCAC	20	7.0284103E-4	45.0	36
TCCAACG	25	3.8867627E-5	45.0	15