##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933874.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432918
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37037730008916	33.0	31.0	34.0	31.0	34.0
2	32.596810481430666	34.0	31.0	34.0	31.0	34.0
3	32.65151830138733	34.0	31.0	34.0	31.0	34.0
4	36.05682138418823	37.0	35.0	37.0	35.0	37.0
5	36.08740916293617	37.0	35.0	37.0	35.0	37.0
6	35.701183595969674	37.0	35.0	37.0	35.0	37.0
7	36.03500431952472	37.0	35.0	37.0	35.0	37.0
8	36.03092964487501	37.0	35.0	37.0	35.0	37.0
9	37.86432072586494	39.0	38.0	39.0	35.0	39.0
10	37.558953427669906	39.0	37.0	39.0	35.0	39.0
11	37.50135591497697	39.0	37.0	39.0	35.0	39.0
12	37.28804300121501	39.0	37.0	39.0	35.0	39.0
13	37.14891041721527	39.0	37.0	39.0	33.0	39.0
14	38.266498505490645	40.0	38.0	41.0	33.0	41.0
15	38.34885590342744	40.0	38.0	41.0	34.0	41.0
16	38.47687090857853	40.0	38.0	41.0	34.0	41.0
17	38.461071149732746	40.0	37.0	41.0	34.0	41.0
18	38.430633514891966	40.0	37.0	41.0	34.0	41.0
19	38.45983303997524	40.0	37.0	41.0	34.0	41.0
20	38.37890778392213	40.0	37.0	41.0	34.0	41.0
21	38.27382090834754	40.0	37.0	41.0	34.0	41.0
22	38.22436812514148	40.0	37.0	41.0	34.0	41.0
23	38.08797047015832	40.0	36.0	41.0	34.0	41.0
24	37.995488753066404	40.0	36.0	41.0	34.0	41.0
25	37.81605292457232	40.0	36.0	41.0	33.0	41.0
26	37.68251031373147	40.0	36.0	41.0	33.0	41.0
27	37.64051621785188	40.0	36.0	41.0	33.0	41.0
28	37.55423659907881	39.0	36.0	41.0	33.0	41.0
29	37.539298435269494	39.0	36.0	41.0	33.0	41.0
30	37.47555195210178	39.0	36.0	41.0	33.0	41.0
31	37.31147700026333	39.0	35.0	41.0	32.0	41.0
32	37.20516356446255	39.0	35.0	41.0	32.0	41.0
33	37.06245986537866	39.0	35.0	41.0	31.0	41.0
34	37.10686088358534	39.0	35.0	41.0	31.0	41.0
35	37.09334562203466	39.0	35.0	41.0	31.0	41.0
36	37.069578996484324	39.0	35.0	41.0	32.0	41.0
37	37.01910061489705	39.0	35.0	41.0	31.0	41.0
38	37.019059036584295	39.0	35.0	41.0	32.0	41.0
39	37.035362354995634	39.0	35.0	41.0	32.0	41.0
40	36.94232626040035	39.0	35.0	41.0	31.0	41.0
41	36.81549854706896	39.0	35.0	41.0	31.0	41.0
42	36.78411385065994	39.0	35.0	41.0	31.0	41.0
43	36.647339680955746	39.0	35.0	41.0	31.0	41.0
44	36.53317487376362	39.0	35.0	41.0	31.0	41.0
45	36.58017222661105	39.0	35.0	40.0	31.0	41.0
46	36.50183637547988	39.0	35.0	40.0	31.0	41.0
47	36.45115703204764	38.0	35.0	40.0	31.0	41.0
48	36.46834504455809	38.0	35.0	40.0	31.0	41.0
49	36.54064973043394	39.0	35.0	40.0	31.0	41.0
50	36.47497216562952	38.0	35.0	40.0	31.0	41.0
51	35.43662079192826	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	15.0
11	8.0
12	9.0
13	4.0
14	9.0
15	7.0
16	12.0
17	27.0
18	53.0
19	123.0
20	171.0
21	316.0
22	499.0
23	780.0
24	1120.0
25	1650.0
26	2235.0
27	2903.0
28	3441.0
29	4513.0
30	5718.0
31	7518.0
32	10246.0
33	15093.0
34	27589.0
35	38341.0
36	34235.0
37	56814.0
38	101550.0
39	117837.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.342443603638564	4.564143787045122	54.1876290660125	15.905783543303812
2	20.365288576589563	5.963715992405028	52.1246517816307	21.54634364937471
3	20.09710845933872	6.260538947329517	51.681842750821176	21.960509842510593
4	19.80629126070064	4.74500944751662	50.74124892011882	24.707450371663917
5	19.270162016825356	6.109702068290069	49.42206145274625	25.198074462138326
6	21.6022433809636	7.191431171723052	52.374814630022314	18.831510817291033
7	81.40571655602216	2.301128620200592	10.761391302740934	5.531763521036316
8	81.48332940649269	4.021315815004227	8.405517904083453	6.089836874419636
9	75.05555324564929	5.9068922983105345	11.447433463150066	7.590120992890108
10	38.51930388664828	27.185517811687205	18.674437191338775	15.620741110325742
11	28.706822077160112	24.476228754637138	27.292697462336978	19.524251705865776
12	27.055470089023785	20.171256450413242	31.946927593678247	20.826345866884722
13	23.456405139079457	24.609510346070156	32.17838020133143	19.755704313518958
14	19.30827547018142	27.52599799500136	31.675975588910603	21.489750945906614
15	15.974849740597527	24.213361421793504	39.03025515224592	20.781533685363048
16	18.76845037628373	23.818598441275256	33.442591899620716	23.970359282820304
17	19.140807266041143	23.95465192022508	30.007299303794248	26.89724150993953
18	20.422574251936858	23.692015578007844	32.68193976688426	23.20347040317104
19	22.9378311828106	25.093897689631756	30.234132098919424	21.734139028638218
20	24.419174069916245	26.592333883091023	29.384086593766025	19.604405453226708
21	22.902258626344942	26.736934015217663	30.315671790038763	20.045135568398635
22	20.83142766066553	24.451512757612296	29.075945098147916	25.641114483574256
23	20.622843125025987	26.159919430469515	28.178315523956037	25.03892192054846
24	20.87854974845121	24.911415094775453	29.408571600164464	24.801463556608873
25	20.138455781464387	26.0924701675606	28.477217394518128	25.29185665645688
26	18.25357227003728	26.83926286271303	30.129031363907256	24.778133503342435
27	19.378727611233536	26.220438974586415	30.66793249529934	23.732900918880713
28	16.53015120646404	24.384294485329786	33.87824022101183	25.207314087194344
29	17.787895167214117	22.94406792972341	33.15408460724664	26.113952295815835
30	20.008177068174575	23.785335791073596	31.513358187924734	24.693128952827095
31	20.654257850216435	25.224869374800768	29.015194563404616	25.105678211578176
32	21.208866344203752	24.583639395913316	28.859506881210763	25.347987378672173
33	19.89522265186479	23.3619299728817	28.99325045389658	27.749596921356932
34	18.184506072743567	21.34722972941758	31.951547406206256	28.516716791632597
35	17.19632817300274	22.61467529647647	30.87582405905968	29.313172471461108
36	19.870737645466345	22.383684670076086	32.060574981867234	25.68500270259033
37	17.728299585602816	24.10826068678133	31.853145399359693	26.310294328256163
38	17.788357148466915	24.643465968151013	30.74208048637386	26.826096397008207
39	18.89618819268314	22.53613848350034	30.768875399036304	27.79879792478021
40	20.604825856166755	21.199395728521335	32.479129996904724	25.71664841840718
41	17.753939545133257	21.392503892192057	30.559828882143965	30.293727680530726
42	18.864311486239888	21.32828849805275	31.26758416143473	28.539815854272632
43	20.04836943716824	21.00813548986182	30.023468647642275	28.92002642532766
44	18.933839664786404	22.436119542268976	30.861040658970058	27.769000133974565
45	19.135494481633934	21.720279591054194	29.249650049201005	29.894575878110867
46	20.470389311601735	22.03211693669471	32.127331272896946	25.370162478806613
47	16.69854337310992	21.518624774206664	34.85879543008144	26.924036422601972
48	17.200024023025147	21.367094923288015	33.039975237804846	28.392905815881992
49	19.073127012505832	19.40552252389598	34.00020327175123	27.521147191846957
50	18.723407204135654	19.759631154167764	31.84944954933729	29.667512092359296
51	17.317367261236537	20.509195736837	29.407185656406064	32.766251345520395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	192.5
2	336.0
3	766.5
4	1197.0
5	813.5
6	430.0
7	513.0
8	596.0
9	618.5
10	641.0
11	682.0
12	723.0
13	691.5
14	660.0
15	614.0
16	568.0
17	583.5
18	599.0
19	813.0
20	1027.0
21	1089.5
22	1152.0
23	1352.5
24	1553.0
25	1979.5
26	2683.0
27	2960.0
28	3898.5
29	4837.0
30	5595.0
31	6353.0
32	7198.0
33	8043.0
34	9274.5
35	10506.0
36	11610.5
37	12715.0
38	14052.0
39	15389.0
40	18184.0
41	20979.0
42	25009.5
43	29040.0
44	31353.5
45	33667.0
46	41020.5
47	48374.0
48	52918.0
49	57462.0
50	53693.5
51	49925.0
52	41503.0
53	33081.0
54	27661.5
55	22242.0
56	19290.0
57	16338.0
58	15202.5
59	14067.0
60	12355.5
61	10644.0
62	9128.5
63	7613.0
64	6604.5
65	5596.0
66	4760.5
67	3925.0
68	3433.0
69	2941.0
70	2191.5
71	1442.0
72	1349.5
73	1257.0
74	1069.0
75	590.5
76	300.0
77	251.0
78	202.0
79	166.5
80	131.0
81	93.0
82	55.0
83	31.5
84	8.0
85	4.5
86	1.0
87	1.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432918.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.79483830213054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48004232158637	23.113815914637424
2	12.281484445760057	8.05538593017665
3	4.99276912761227	4.912111686597413
4	2.4756947210572187	3.2476003225003867
5	1.5387125628517797	2.52309148460905
6	0.9651320405473719	1.8990809525973817
7	0.6535542770160703	1.5003244785485501
8	0.5579543905858391	1.4638419215381104
9	0.41302649922172463	1.2190623530824356
>10	5.364906970656587	41.06948719206721
>50	0.21853993352884057	4.478200143079318
>100	0.05037771194983014	2.966385836527677
>500	0.004257271432380011	1.0676010525563997
>1k	0.00354772619365001	2.4840107314820328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	2808	0.648621678932269	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2729	0.6303734194466388	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2102	0.48554229669360016	TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1998	0.4615192715479606	TruSeq Adapter, Index 16 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	1038	0.23976827020359515	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	971	0.22429189823476964	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	842	0.19449410742912052	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	756	0.1746289135586878	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	727	0.16793018539307675	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	666	0.15383975718265352	TruSeq Adapter, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	626	0.1446001321266383	TruSeq Adapter, Index 27 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	491	0.11341639756258691	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6198125280076137E-4	0.0	0.0	0.6920479166955404	0.0
2	4.6198125280076137E-4	0.0	0.0	2.4210127553023897	0.0
3	4.6198125280076137E-4	0.0	0.0	3.4299798114192526	0.0
4	4.6198125280076137E-4	0.0	0.0	4.49646353350981	0.0
5	4.6198125280076137E-4	0.0	0.0	6.337458825920844	0.0
6	4.6198125280076137E-4	0.0	0.0	7.3572824414785245	0.0
7	4.6198125280076137E-4	0.0	0.0	8.373872188266601	0.0
8	4.6198125280076137E-4	0.0	0.0	10.081354898618214	0.0
9	4.6198125280076137E-4	0.0	0.0	10.985221219722904	0.0
10	4.6198125280076137E-4	0.0	0.0	12.710490208307347	0.0
11	4.6198125280076137E-4	0.0	0.0	15.301050083387617	0.0
12	4.6198125280076137E-4	0.0	0.0	17.786740214082112	0.0
13	4.6198125280076137E-4	0.0	0.0	18.576959146997815	0.0
14	4.6198125280076137E-4	0.0	0.0	18.855302851810272	0.0
15	4.6198125280076137E-4	0.0	0.0	19.303424667027013	0.0
16	4.6198125280076137E-4	0.0	0.0	20.299918229318255	0.0
17	4.6198125280076137E-4	0.0	0.0	21.578913327697162	0.0
18	4.6198125280076137E-4	0.0	0.0	23.121237740172504	0.0
19	4.6198125280076137E-4	0.0	0.0	24.107105733649327	0.0
20	4.6198125280076137E-4	0.0	0.0	25.125081424195805	0.0
21	4.6198125280076137E-4	0.0	0.0	26.450736629107592	0.0
22	4.6198125280076137E-4	0.0	0.0	27.75929852766575	0.0
23	4.6198125280076137E-4	0.0	0.0	28.988399650742174	0.0
24	4.6198125280076137E-4	0.0	0.0	29.92067781889411	0.0
25	4.6198125280076137E-4	0.0	0.0	30.702581089259397	0.0
26	4.6198125280076137E-4	0.0	0.0	31.429739581167798	0.0
27	4.6198125280076137E-4	0.0	0.0	32.14881340115218	0.0
28	4.6198125280076137E-4	0.0	0.0	32.83370060842931	0.0
29	4.6198125280076137E-4	0.0	0.0	33.629925297631424	0.0
30	4.6198125280076137E-4	0.0	0.0	34.479046840279224	0.0
31	4.6198125280076137E-4	0.0	0.0	35.292595826461366	0.0
32	4.6198125280076137E-4	0.0	0.0	36.022526205886564	0.0
33	4.6198125280076137E-4	0.0	0.0	36.73513228833174	0.0
34	4.6198125280076137E-4	0.0	0.0	37.45559205207453	0.0
35	4.6198125280076137E-4	0.0	0.0	38.24650395686943	0.0
36	4.6198125280076137E-4	0.0	0.0	38.94501961110418	0.0
37	4.6198125280076137E-4	0.0	0.0	39.631523752766114	0.0
38	4.6198125280076137E-4	0.0	0.0	40.33535219140807	0.0
39	4.6198125280076137E-4	0.0	0.0	41.301124000388064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	35	1.2095734E-7	45.000004	5
GTAGCGG	35	1.2095734E-7	45.000004	2
TTTAGCG	25	3.8867627E-5	45.0	1
TCGTTCG	45	3.8380676E-10	45.0	1
GGTACAT	20	7.0284103E-4	45.0	8
GGTCGCA	20	7.0284103E-4	45.0	23
TTAGCGA	20	7.0284103E-4	45.0	29
GCAACGA	20	7.0284103E-4	45.0	13
CAACCCG	20	7.0284103E-4	45.0	23
TTCCGTA	20	7.0284103E-4	45.0	1
AGGAACG	20	7.0284103E-4	45.0	10
CGTTCTA	20	7.0284103E-4	45.0	29
AAACGGC	20	7.0284103E-4	45.0	16
TGACCGT	20	7.0284103E-4	45.0	16
GGAACGA	25	3.8867627E-5	45.0	8
TCGTCGT	20	7.0284103E-4	45.0	31
CTGCAAC	20	7.0284103E-4	45.0	27
AACGAGG	20	7.0284103E-4	45.0	10
GTATCAC	20	7.0284103E-4	45.0	36
TCCAACG	25	3.8867627E-5	45.0	15
>>END_MODULE