##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933873.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126305
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70382011796841	34.0	31.0	34.0	31.0	34.0
2	32.94127706741617	34.0	33.0	34.0	31.0	34.0
3	33.03212857764934	34.0	33.0	34.0	31.0	34.0
4	36.28418510747793	37.0	37.0	37.0	35.0	37.0
5	36.27838169510312	37.0	37.0	37.0	35.0	37.0
6	35.91219666679862	37.0	36.0	37.0	35.0	37.0
7	35.99943786865128	37.0	36.0	37.0	35.0	37.0
8	36.13049364633229	37.0	37.0	37.0	35.0	37.0
9	37.90081152765132	39.0	39.0	39.0	35.0	39.0
10	37.83666521515379	39.0	38.0	39.0	35.0	39.0
11	37.87287122441708	39.0	38.0	39.0	35.0	39.0
12	37.74497446656902	39.0	37.0	39.0	35.0	39.0
13	37.65588060646847	39.0	37.0	39.0	35.0	39.0
14	39.01654724674399	40.0	38.0	41.0	35.0	41.0
15	39.065611020941375	40.0	38.0	41.0	35.0	41.0
16	39.18770436641463	40.0	39.0	41.0	35.0	41.0
17	39.07986223823285	40.0	38.0	41.0	35.0	41.0
18	39.03950754126915	40.0	38.0	41.0	35.0	41.0
19	39.04656981117137	40.0	38.0	41.0	35.0	41.0
20	38.96285974426982	40.0	38.0	41.0	35.0	41.0
21	38.95400023752029	40.0	38.0	41.0	35.0	41.0
22	38.9607616483908	40.0	38.0	41.0	35.0	41.0
23	38.818019872530776	40.0	38.0	41.0	35.0	41.0
24	38.77442698230474	40.0	38.0	41.0	35.0	41.0
25	38.732607576897195	40.0	38.0	41.0	35.0	41.0
26	38.66846126439967	40.0	38.0	41.0	35.0	41.0
27	38.606484303867624	40.0	38.0	41.0	35.0	41.0
28	38.506907881714895	40.0	38.0	41.0	35.0	41.0
29	38.49202327698824	40.0	38.0	41.0	35.0	41.0
30	38.43921459958038	40.0	38.0	41.0	35.0	41.0
31	38.390024147895964	40.0	38.0	41.0	35.0	41.0
32	38.26477178258976	40.0	38.0	41.0	35.0	41.0
33	38.173651082696644	40.0	38.0	41.0	34.0	41.0
34	38.10928308459681	40.0	38.0	41.0	34.0	41.0
35	38.02612723170104	40.0	37.0	41.0	34.0	41.0
36	37.93380309568109	40.0	37.0	41.0	34.0	41.0
37	37.91065278492538	40.0	37.0	41.0	34.0	41.0
38	37.83110724041012	40.0	37.0	41.0	34.0	41.0
39	37.800419619175806	40.0	37.0	41.0	34.0	41.0
40	37.692688333795175	40.0	37.0	41.0	34.0	41.0
41	37.562487629151654	40.0	36.0	41.0	33.0	41.0
42	37.55818059459246	40.0	36.0	41.0	33.0	41.0
43	37.380840030085906	40.0	36.0	41.0	33.0	41.0
44	37.35692965440798	40.0	36.0	41.0	33.0	41.0
45	37.379098214639164	40.0	36.0	41.0	33.0	41.0
46	37.2681287359962	40.0	35.0	41.0	33.0	41.0
47	37.144887375796685	39.0	35.0	41.0	33.0	41.0
48	37.12484858081628	39.0	35.0	41.0	33.0	41.0
49	37.13077075333518	39.0	35.0	41.0	33.0	41.0
50	37.03183563596057	39.0	35.0	41.0	33.0	41.0
51	36.384276156921736	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	0.0
14	0.0
15	4.0
16	8.0
17	9.0
18	18.0
19	42.0
20	113.0
21	181.0
22	268.0
23	382.0
24	405.0
25	562.0
26	656.0
27	744.0
28	737.0
29	822.0
30	951.0
31	1131.0
32	1513.0
33	2305.0
34	5374.0
35	8177.0
36	7041.0
37	11968.0
38	27010.0
39	55835.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.844582558093503	4.65856458572503	54.88302125806579	14.613831598115674
2	20.281857408653657	8.698784687858755	50.52056529828589	20.498792605201693
3	20.37211511816634	8.665531847511975	48.20078381695103	22.76156921737065
4	18.718182178061042	5.258699180555006	51.40651597323939	24.616602668144573
5	22.05930089861842	6.036973991528443	46.84929337714263	25.0544317327105
6	21.43462254067535	10.26404338703931	50.17220220893868	18.12913186334666
7	80.68326669569691	2.642017338981038	12.113534697755433	4.561181267566605
8	81.60088674240924	6.526265785202486	7.292664581766359	4.5801828906219075
9	75.35251969439057	5.994220339654012	9.469142155892483	9.184117810062943
10	38.04283282530383	29.2506234907565	17.12046237282768	15.586081311111991
11	31.550611614742092	24.953881477376193	24.678357943074307	18.817148964807412
12	28.873757966826336	20.825778868611692	28.564981592177663	21.73548157238431
13	25.323621392660623	22.524048929179365	31.492815011282214	20.659514666877797
14	20.71493606745576	27.06939551086655	29.096235303432167	23.119433118245517
15	17.531372471398598	24.84066347333835	36.03499465579352	21.59296939946954
16	20.225644273781718	23.900083132100868	32.86726574561577	23.007006848501643
17	19.76406318039666	24.38224931712917	31.418391987648945	24.435295514825224
18	20.756897985036222	24.446379794940817	30.337674676378608	24.459047543644353
19	22.875578955702466	26.55397648549147	28.56656506076561	22.003879498040458
20	27.048018684929335	25.233363683147935	29.49289418471161	18.225723447211113
21	24.4305451090614	27.835002573136457	27.3868809627489	20.347571355053244
22	21.581093385059972	24.425794703297573	29.119195597957326	24.873916313685125
23	22.079094255967696	27.582439333359726	28.41059340485333	21.927873005819247
24	22.655476821978542	23.749653616246388	28.899093464233406	24.695776097541668
25	21.31902933375559	27.338585170816675	27.204782075135586	24.13760342029215
26	20.570840425953048	26.645817663592098	29.171450061359405	23.611891849095443
27	21.170975020783025	27.992557697636673	28.76370689996437	22.07276038161593
28	18.912948814377895	27.077312853806262	31.907683781322987	22.102054550492856
29	20.716519536043705	24.963382288903844	30.656743596848894	23.663354578203556
30	21.83761529630656	25.553224337912194	30.363801908079648	22.245358457701595
31	21.886702822532758	27.220616761015005	26.340999960413285	24.551680456038955
32	23.56755472863307	27.758996080915242	26.60464748030561	22.068801710146076
33	23.100431495190215	24.997426863544593	26.644234195004156	25.257907446261036
34	22.379161553382684	25.157357190926728	28.460472665373498	24.00300859031709
35	21.799612050195954	23.4986738450576	28.896718261351488	25.804995843394956
36	20.123510549859468	26.95934444400459	29.948933138038875	22.968211868097065
37	20.247021099718935	26.291120699893117	29.9536835438027	23.50817465658525
38	22.09730414472903	26.987055144293574	26.408297375400814	24.507343335576582
39	22.538300146470842	23.444835913067575	27.35204465381418	26.6648192866474
40	25.22782154309014	22.284945172400143	29.720121927081273	22.767111357428448
41	20.384782866869877	23.767863505007718	30.165868334586914	25.681485293535488
42	22.805906337833022	24.07980681683227	29.804837496536162	23.309449348798545
43	21.630972645580144	23.6158505205653	30.578361901745776	24.174814932108784
44	21.584260322235856	24.337120462372827	29.336922528799334	24.74169668659198
45	21.40295316891651	22.882704564348206	28.127944261905704	27.586398004829576
46	22.494754760302442	24.95942361743399	28.314001820988878	24.231819801274693
47	19.060211393056488	24.634812556905903	32.674874312180826	23.630101737856776
48	20.57717430030482	24.012509401844742	30.76679466371086	24.643521634139585
49	21.363366454217964	21.786152567198446	32.04782075135584	24.802660227227744
50	21.151973397727723	22.137682593721546	29.731206207196863	26.979137801353865
51	20.121927081271526	21.91362178852777	28.06381378409406	29.900637346106645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	97.5
2	152.0
3	325.5
4	499.0
5	356.0
6	213.0
7	218.0
8	223.0
9	221.0
10	219.0
11	212.0
12	205.0
13	219.0
14	233.0
15	219.5
16	206.0
17	227.5
18	249.0
19	272.5
20	296.0
21	342.5
22	389.0
23	495.0
24	601.0
25	698.5
26	1040.5
27	1285.0
28	1415.5
29	1546.0
30	1855.0
31	2164.0
32	2353.5
33	2543.0
34	2984.5
35	3426.0
36	3674.5
37	3923.0
38	4329.5
39	4736.0
40	5259.0
41	5782.0
42	6577.5
43	7373.0
44	8002.0
45	8631.0
46	11300.0
47	13969.0
48	13621.5
49	13274.0
50	12696.5
51	12119.0
52	10600.0
53	9081.0
54	7887.0
55	6693.0
56	6248.5
57	5804.0
58	5524.0
59	5244.0
60	4975.5
61	4707.0
62	4105.0
63	3503.0
64	2867.0
65	2231.0
66	1775.5
67	1320.0
68	1084.5
69	849.0
70	759.0
71	669.0
72	578.0
73	487.0
74	376.0
75	211.0
76	157.0
77	125.0
78	93.0
79	77.0
80	61.0
81	49.5
82	38.0
83	20.5
84	3.0
85	2.5
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	126305.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.52994734966945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25303574862079	38.67701199477455
2	11.153510523435536	11.940936621669767
3	5.669195840913461	9.104152646371876
4	3.7789708775199298	8.09152448438304
5	2.48776086731449	6.658485412295634
6	1.6550561299196878	5.315704049720914
7	1.0856221620742188	4.0679308024227065
8	0.6315540370649747	2.7045643482047423
9	0.37863661238555857	1.8241558133090534
>10	0.8652438212716865	6.589604528720161
>50	0.019227640472704144	0.767982265151815
>100	0.017748591205573056	1.9927952179248642
>500	0.0029580985342621763	1.256482324531887
>1k	0.0014790492671310882	1.008669490518982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	1274	1.008669490518982	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	958	0.7584814536241637	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	629	0.4980008709077234	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	331	0.2620640513043822	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	301	0.23831202248525396	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGCGTT	299	0.23672855389731207	No Hit
CTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGCT	270	0.21376825937215468	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	250	0.19793357349273585	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	195	0.15438818732433396	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTA	168	0.13301136138711847	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCT	163	0.12905268991726376	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCT	160	0.12667748703535092	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTC	159	0.12588575274137997	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22326907089980602	0.0
2	0.0	0.0	0.0	0.9405803412374807	0.0
3	0.0	0.0	0.0	1.4077035746803372	0.0
4	0.0	0.0	0.0	2.096512410435058	0.0
5	0.0	0.0	0.0	3.493923439293773	0.0
6	0.0	0.0	0.0	4.2793238589129485	0.0
7	0.0	0.0	0.0	5.039388781125054	0.0
8	0.0	0.0	0.0	6.229365424963382	0.0
9	0.0	0.0	0.0	6.792288507976723	0.0
10	0.0	0.0	0.0	8.580024543763113	0.0
11	0.0	0.0	0.0	10.344800285024347	0.0
12	0.0	0.0	0.0	13.046989430347175	0.0
13	0.0	0.0	0.0	13.703337160049088	0.0
14	0.0	0.0	0.0	13.927397965242864	0.0
15	0.0	0.0	0.0	14.344641938165552	0.0
16	0.0	0.0	0.0	15.077787894382645	0.0
17	0.0	0.0	0.0	15.982740192391434	0.0
18	0.0	0.0	0.0	17.02070385178734	0.0
19	0.0	0.0	0.0	17.97078500455247	0.0
20	0.0	0.0	0.0	18.802106013221962	0.0
21	0.0	0.0	0.0	19.88044812161039	0.0
22	0.0	0.0	0.0	21.075175171212543	0.0
23	0.0	0.0	0.0	22.102846284786825	0.0
24	0.0	0.0	0.0	22.907248327461303	0.0
25	0.0	0.0	0.0	23.578639008748663	0.0
26	0.0	0.0	0.0	24.18906614940026	0.0
27	0.0	0.0	0.0	24.86758243933336	0.0
28	0.0	0.0	0.0	25.499386405922174	0.0
29	0.0	0.0	0.0	26.16206800997585	0.0
30	0.0	0.0	0.0	26.84929337714263	0.0
31	0.0	0.0	0.0	27.62994339099798	0.0
32	0.0	0.0	0.0	28.34250425557183	0.0
33	0.0	0.0	0.0	29.027354419856696	0.0
34	0.0	0.0	0.0	29.654407980681682	0.0
35	0.0	0.0	0.0	30.342425082142434	0.0
36	0.0	0.0	0.0	31.032025652191123	0.0
37	0.0	0.0	0.0	31.815050868928388	0.0
38	0.0	0.0	0.0	32.5204861248565	0.0
39	0.0	0.0	0.0	33.20771149202328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACCG	35	1.2004784E-7	45.000004	8
TTGTGCG	25	3.8710496E-5	45.0	1
CGAAAAA	20	7.009435E-4	45.0	22
CCGATGA	50	2.1827873E-11	45.0	18
ATAGCAA	20	7.009435E-4	45.0	10
TGCGGGT	25	3.8710496E-5	45.0	4
TACGAAA	20	7.009435E-4	45.0	20
GCGATTC	40	6.73208E-9	45.0	9
CGATGAA	50	2.1827873E-11	45.0	19
ACATACG	20	7.009435E-4	45.0	17
AACACAC	20	7.009435E-4	45.0	27
TCTTATG	30	2.1501346E-6	44.999996	1
CGGGAAC	30	2.1501346E-6	44.999996	6
TGGGCCG	30	2.1501346E-6	44.999996	6
GGACCAA	30	2.1501346E-6	44.999996	8
TGCGGGA	210	0.0	42.857147	4
TGGGCGA	155	0.0	42.09677	6
GTGCGGG	75	0.0	42.0	3
CGGGAAT	65	0.0	41.53846	6
TACGGCT	180	0.0	41.25	7
>>END_MODULE