##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225795
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44233742183644	33.0	31.0	34.0	31.0	34.0
2	32.653967425222	34.0	31.0	34.0	31.0	34.0
3	32.71739809674538	34.0	31.0	34.0	31.0	34.0
4	36.0830244861498	37.0	35.0	37.0	35.0	37.0
5	36.097694149511135	37.0	35.0	37.0	35.0	37.0
6	35.706408494079355	37.0	35.0	37.0	35.0	37.0
7	36.02649219486129	37.0	35.0	37.0	35.0	37.0
8	36.04196949734662	37.0	36.0	37.0	35.0	37.0
9	37.892868709694525	39.0	38.0	39.0	35.0	39.0
10	37.599285361744826	39.0	37.0	39.0	35.0	39.0
11	37.531183436055784	39.0	37.0	39.0	35.0	39.0
12	37.23073515555211	39.0	37.0	39.0	34.0	39.0
13	37.078570234011394	39.0	37.0	39.0	33.0	39.0
14	37.98216014912771	40.0	37.0	41.0	33.0	41.0
15	38.138933508457775	40.0	37.0	41.0	33.0	41.0
16	38.348535440265294	40.0	37.0	41.0	33.0	41.0
17	38.36417182318414	40.0	37.0	41.0	34.0	41.0
18	38.338162580203054	40.0	37.0	41.0	34.0	41.0
19	38.343232759148144	40.0	37.0	41.0	34.0	41.0
20	38.24242797531398	40.0	37.0	41.0	34.0	41.0
21	38.1770279696034	40.0	37.0	41.0	34.0	41.0
22	38.232873359737965	40.0	37.0	41.0	34.0	41.0
23	38.2100408306446	40.0	37.0	41.0	34.0	41.0
24	38.1741392320902	40.0	36.0	41.0	34.0	41.0
25	38.06737831366583	40.0	36.0	41.0	34.0	41.0
26	37.99127341847536	40.0	36.0	41.0	34.0	41.0
27	37.969490004446094	40.0	36.0	41.0	34.0	41.0
28	37.860393458938894	40.0	36.0	41.0	34.0	41.0
29	37.82649545804967	40.0	36.0	41.0	34.0	41.0
30	37.75290403370874	40.0	36.0	41.0	33.0	41.0
31	37.633257600169685	40.0	35.0	41.0	33.0	41.0
32	37.55750594512133	40.0	35.0	41.0	33.0	41.0
33	37.49836636631737	40.0	35.0	41.0	33.0	41.0
34	37.370445302844274	40.0	35.0	41.0	32.0	41.0
35	37.316063452698046	40.0	35.0	41.0	32.0	41.0
36	37.21151987077774	39.0	35.0	41.0	32.0	41.0
37	37.08664009887461	39.0	35.0	41.0	32.0	41.0
38	37.01912065231136	39.0	35.0	41.0	32.0	41.0
39	37.00562084198418	39.0	35.0	41.0	32.0	41.0
40	36.881597657030746	39.0	35.0	41.0	32.0	41.0
41	36.73463181037612	39.0	35.0	41.0	31.0	41.0
42	36.711078116650825	39.0	35.0	41.0	31.0	41.0
43	36.60502531010487	39.0	35.0	41.0	31.0	41.0
44	36.5024624835311	39.0	35.0	41.0	31.0	41.0
45	36.53693562137225	39.0	35.0	41.0	31.0	41.0
46	36.4939871675117	38.0	35.0	41.0	31.0	41.0
47	36.389806615298646	38.0	35.0	40.0	31.0	41.0
48	36.279164950093616	38.0	35.0	40.0	31.0	41.0
49	36.238121382449755	38.0	35.0	40.0	31.0	41.0
50	36.11946287919269	38.0	35.0	40.0	31.0	41.0
51	35.116085479219606	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	12.0
10	26.0
11	20.0
12	13.0
13	22.0
14	24.0
15	42.0
16	85.0
17	156.0
18	217.0
19	406.0
20	786.0
21	1283.0
22	1850.0
23	2560.0
24	3830.0
25	5266.0
26	7188.0
27	8791.0
28	10375.0
29	12828.0
30	15906.0
31	21153.0
32	29114.0
33	45298.0
34	89531.0
35	88512.0
36	88250.0
37	142294.0
38	257417.0
39	392270.0
40	267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.68936486117173	4.432633515392052	55.52208974583841	15.355911877597803
2	20.221978389534957	5.2793493202370705	52.7462585505733	21.75241373965467
3	21.72557401523093	5.127529480867519	50.701136813251814	22.44575969064974
4	19.879343609657408	4.957843685118638	50.18204512173732	24.980767583486635
5	19.47560562736836	6.165060226220534	48.9522310011054	25.407103145305697
6	22.041369070684738	7.202590971573549	51.83729742738387	18.91874253035785
7	83.12009756933256	2.5960295155388953	9.574521025130629	4.70935188999792
8	84.85668484534527	3.4644455231094926	7.111874334615495	4.566995296929748
9	78.62970561961828	5.456948347806934	9.807349516028372	6.105996516546405
10	42.47627050200074	26.160410182779337	16.545833520286834	14.817485794933082
11	33.453554631891954	23.47121663899755	24.65714087592134	18.418087853189157
12	31.66916164611538	19.30575667220049	28.55722204773229	20.467859633951843
13	27.890960560289447	19.949828478660788	31.122414433082202	21.036796527967564
14	21.5932517264306	22.338971850921236	33.73647306441942	22.33130335822874
15	19.719202639919402	22.574818791070285	36.68199005543341	21.023988513576903
16	25.324952377844586	22.291900358542822	30.769908508355805	21.61323875525679
17	25.523925289302046	22.494952255475017	28.231637427139123	23.749485028083814
18	25.80643582328203	22.813765760180125	29.519291561802746	21.86050685473509
19	26.37357796368887	24.81875028043025	27.505904331474675	21.3017674244062
20	25.6086050277575	27.786212213298306	27.710016764630303	18.895165994313896
21	25.307657479431718	25.27755456662819	30.015051456401764	19.399736497538335
22	23.0553232799938	22.448288661644074	31.094024694178064	23.40236336418406
23	22.762044224360515	23.894125853017837	30.12355247002965	23.220277452591993
24	23.57816763814504	24.964125322749727	28.071006979144148	23.386700059961086
25	24.934838207041143	25.12442945190672	25.469022144812143	24.47171019623999
26	22.59366370396355	25.35578950803356	26.222818660542753	25.827728127460137
27	22.933606353427777	24.61333257192271	29.348545229830435	23.104515844819076
28	20.935148209937225	25.85016254757117	28.157073572661005	25.057615669830604
29	23.164069032750174	26.16171545813125	26.789879221240092	23.88433628787848
30	25.865173214118187	24.332779950970597	27.04595792934381	22.756088905567406
31	24.845671584563487	25.608115549500525	27.334668521245398	22.21154434469059
32	27.62566334501283	26.133733617774585	24.437773037090217	21.80283000012237
33	27.800651821878862	24.416562312621604	24.77559461410758	23.007191251391955
34	23.40954237861959	25.128508437381452	28.944644088122402	22.51730509587655
35	24.26490563267104	27.228043840935882	25.425213840813516	23.081836685579564
36	26.563250788263943	26.87513001766201	24.245163342973335	22.316455851100713
37	23.94601054825644	29.07468214505688	25.7886514466122	21.190655860074482
38	23.540885710905986	27.94398737146097	25.417300608992534	23.097826308640514
39	22.858471440983198	24.638785441285044	26.558192846275276	25.944550271456485
40	24.407751703996183	22.78260231115317	29.547110242740427	23.26253574211022
41	22.36719843040639	24.269800415240724	27.79534914076171	25.56765201359118
42	22.993893758744324	24.334329965450994	28.78890842269711	23.882867853107577
43	23.53117772547612	23.935976243988595	28.502318903242386	24.0305271272929
44	23.7390428252685	23.54047781235851	27.516836012546957	25.20364334982603
45	22.620666587806284	22.7913313400691	25.989990169645004	28.598011902479612
46	22.775994354684105	23.941442084524738	27.93403464690262	25.348528913888536
47	21.347615221142195	24.628424818179223	30.55527229267537	23.468687668003213
48	21.216108729436815	26.4815079193503	28.530790221856016	23.771593129356866
49	23.857822882292716	24.088775039872083	28.416007570597042	23.63739450723816
50	21.679236740237968	23.897389041397624	27.802528155197237	26.62084606316717
51	20.669279936694146	24.408893819929105	26.493908035193485	28.42791820818326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	669.0
2	1209.0
3	2862.0
4	4515.0
5	3043.0
6	1571.0
7	1559.5
8	1548.0
9	1606.5
10	1665.0
11	1696.5
12	1728.0
13	1740.0
14	1752.0
15	1787.5
16	1823.0
17	1758.5
18	1694.0
19	2086.5
20	2479.0
21	2624.5
22	2770.0
23	3486.0
24	4202.0
25	5119.0
26	8049.5
27	10063.0
28	11561.5
29	13060.0
30	16261.5
31	19463.0
32	21600.0
33	23737.0
34	26269.0
35	28801.0
36	30406.0
37	32011.0
38	35920.0
39	39829.0
40	44493.0
41	49157.0
42	53319.5
43	57482.0
44	63527.5
45	69573.0
46	85495.5
47	101418.0
48	113676.0
49	125934.0
50	125583.5
51	125233.0
52	110891.5
53	96550.0
54	88092.5
55	79635.0
56	77180.0
57	74725.0
58	69923.0
59	65121.0
60	58725.5
61	52330.0
62	45507.0
63	38684.0
64	33297.5
65	27911.0
66	24517.0
67	21123.0
68	18274.0
69	15425.0
70	12704.5
71	9984.0
72	8272.5
73	6561.0
74	5260.5
75	3266.0
76	2572.0
77	1954.5
78	1337.0
79	940.5
80	544.0
81	406.0
82	268.0
83	185.5
84	103.0
85	79.5
86	56.0
87	33.0
88	10.0
89	10.5
90	11.0
91	7.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1225795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.850332464272505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42229104688789	20.60556842062749
2	10.960976889945137	6.324556548162496
3	4.272083430547084	3.697530818591795
4	2.144565057247209	2.4748565957137423
5	1.3261116682314225	1.9129381256613787
6	0.8868232568942058	1.5351087479068068
7	0.7128511165538353	1.439619419707421
8	0.5547790535389613	1.2804448111050775
9	0.4698359629717376	1.2199431361865627
>10	6.833762761729127	47.13695160117153
>50	0.3359366788577334	5.998731202379488
>100	0.07369869200895267	3.716620162500734
>500	0.003142192293367147	0.6111680144742102
>1k	0.003142192293367147	2.045962395811319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	3365	0.2745157224495124	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3290	0.2683972442374133	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3220	0.26268666457278744	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2661	0.2170836069652756	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	0.2124335635240803	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	2078	0.16952263632989203	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1813	0.1479040133138086	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	1701	0.13876708585040728	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1633	0.13321966560477078	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	1427	0.11641424544887197	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.157970949465449E-5	0.0	0.0	0.28699741800219447	0.0
2	8.157970949465449E-5	0.0	0.0	1.0923523101334236	0.0
3	8.157970949465449E-5	0.0	0.0	1.5842779583861901	0.0
4	8.157970949465449E-5	0.0	0.0	2.1634938957982373	0.0
5	8.157970949465449E-5	0.0	0.0	3.2728963652160434	0.0
6	8.157970949465449E-5	0.0	0.0	4.128585938105474	0.0
7	8.157970949465449E-5	0.0	0.0	4.888419352338686	0.0
8	8.157970949465449E-5	0.0	0.0	6.108525487540739	0.0
9	8.157970949465449E-5	0.0	0.0	6.825447974579762	0.0
10	8.157970949465449E-5	0.0	0.0	8.01863280564858	0.0
11	8.157970949465449E-5	0.0	0.0	9.72927773404199	0.0
12	1.6315941898930897E-4	0.0	0.0	11.35703767758883	0.0
13	1.6315941898930897E-4	0.0	0.0	11.916021847046203	0.0
14	1.6315941898930897E-4	0.0	0.0	12.141834482927406	0.0
15	1.6315941898930897E-4	0.0	0.0	12.446616277599436	0.0
16	1.6315941898930897E-4	0.0	0.0	13.011147867302444	0.0
17	1.6315941898930897E-4	0.0	0.0	13.74299944117899	0.0
18	1.6315941898930897E-4	0.0	0.0	14.52591991319919	0.0
19	1.6315941898930897E-4	0.0	0.0	15.14094934307939	0.0
20	1.6315941898930897E-4	0.0	0.0	15.808597685583642	0.0
21	1.6315941898930897E-4	0.0	0.0	16.696592823432955	0.0
22	1.6315941898930897E-4	0.0	0.0	17.671470351894076	0.0
23	1.6315941898930897E-4	0.0	0.0	18.53507315660449	0.0
24	1.6315941898930897E-4	0.0	0.0	19.185997658662338	0.0
25	1.6315941898930897E-4	0.0	0.0	19.78193743652079	0.0
26	1.6315941898930897E-4	0.0	0.0	20.306413388861923	0.0
27	1.6315941898930897E-4	0.0	0.0	20.9278876157922	0.0
28	1.6315941898930897E-4	0.0	0.0	21.481487524422924	0.0
29	1.6315941898930897E-4	0.0	0.0	22.11715662080527	0.0
30	1.6315941898930897E-4	0.0	0.0	22.90040341166345	0.0
31	1.6315941898930897E-4	0.0	0.0	23.502706406862487	0.0
32	1.6315941898930897E-4	0.0	0.0	24.097259329659526	0.0
33	1.6315941898930897E-4	0.0	0.0	24.66521726716131	0.0
34	1.6315941898930897E-4	0.0	0.0	25.306596943208284	0.0
35	1.6315941898930897E-4	0.0	0.0	26.00214554635971	0.0
36	1.6315941898930897E-4	0.0	0.0	26.621825019681104	0.0
37	1.6315941898930897E-4	0.0	0.0	27.255046724778612	0.0
38	1.6315941898930897E-4	0.0	0.0	28.03038028381581	0.0
39	1.6315941898930897E-4	0.0	0.0	29.586023764169376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGA	20	7.0334703E-4	45.0	14
CGTTCAT	100	0.0	40.500004	17
ACATACG	305	0.0	39.09836	17
CGTTTGG	785	0.0	38.694267	2
TTTGGGA	5670	0.0	38.650795	4
TTTGGGC	2360	0.0	38.61229	4
TCGATTG	35	6.248627E-6	38.57143	1
GCAATCG	35	6.248627E-6	38.57143	45
TCGTTTG	410	0.0	38.414635	1
CGGGTAT	130	0.0	38.076927	6
TTGGGAC	1900	0.0	37.776314	5
TTGGGCA	1085	0.0	37.53456	5
CCGAACG	30	1.1399662E-4	37.499996	37
TATGTCG	30	1.1399662E-4	37.499996	1
CATACGA	320	0.0	37.265625	18
TCTTGCG	315	0.0	37.142857	1
CGTTTTT	1380	0.0	36.847824	1
TACGAAA	325	0.0	36.692307	20
TTGGGAT	3185	0.0	36.66405	5
GTTGGGA	1960	0.0	36.6199	4
>>END_MODULE