FastQCFastQC Report
Sat 14 Jan 2017
SRR2933871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949965
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC26700.2810629865310827TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT21590.22727153105640735TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC15740.16569031490633865TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.15074239577247583No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC13390.14095256141015722TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTG207.0326694E-445.01
TCGTTTG4200.042.321431
TTTTGCG7100.040.8802831
CGGGCGA3000.040.5000046
TATTGCG451.927765E-840.01
CGTTTGG8000.039.3752
GGGCGAT15350.039.1368067
TGGGCGA10700.039.112156
TTTTTCG4400.038.8636361
CATACGA2650.038.20754618
TTTCTCG3200.037.968751
TTGGGAT25850.037.775635
TTGGGAC18200.037.7060435
TTTGGGC22700.037.5660824
GGTACGC301.13977265E-437.5000048
CAACGCG301.13977265E-437.50000417
AAGCGTA301.13977265E-437.50000425
TACGGGT301.13977265E-437.5000044
ATCGACG301.13977265E-437.50000416
GCGATGT2350.037.3404279
Produced by FastQC (version 0.11.5)