##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933871.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	949965
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5268994120836	34.0	31.0	34.0	31.0	34.0
2	32.756067855131505	34.0	31.0	34.0	31.0	34.0
3	32.80923507708179	34.0	31.0	34.0	31.0	34.0
4	36.15239824625118	37.0	35.0	37.0	35.0	37.0
5	36.17075576468607	37.0	35.0	37.0	35.0	37.0
6	35.80421594479797	37.0	35.0	37.0	35.0	37.0
7	36.07995031395893	37.0	35.0	37.0	35.0	37.0
8	36.091326522556095	37.0	37.0	37.0	35.0	37.0
9	37.95176980204534	39.0	38.0	39.0	35.0	39.0
10	37.64816177438116	39.0	37.0	39.0	35.0	39.0
11	37.5679493455022	39.0	37.0	39.0	35.0	39.0
12	37.42055128346834	39.0	37.0	39.0	35.0	39.0
13	37.39760728026822	39.0	37.0	39.0	35.0	39.0
14	38.684834704436476	40.0	38.0	41.0	35.0	41.0
15	38.762192291294944	40.0	38.0	41.0	35.0	41.0
16	38.80892453932513	40.0	38.0	41.0	35.0	41.0
17	38.76170174690647	40.0	38.0	41.0	35.0	41.0
18	38.70370908401888	40.0	38.0	41.0	35.0	41.0
19	38.711095671945806	40.0	38.0	41.0	35.0	41.0
20	38.6660992773418	40.0	38.0	41.0	35.0	41.0
21	38.588095350881346	40.0	38.0	41.0	35.0	41.0
22	38.572081076671246	40.0	38.0	41.0	35.0	41.0
23	38.52633307542909	40.0	38.0	41.0	34.0	41.0
24	38.49616775354882	40.0	38.0	41.0	34.0	41.0
25	38.412664677119686	40.0	38.0	41.0	34.0	41.0
26	38.322605569678885	40.0	37.0	41.0	34.0	41.0
27	38.283604132783836	40.0	37.0	41.0	34.0	41.0
28	38.20823504023832	40.0	37.0	41.0	34.0	41.0
29	38.193321859226394	40.0	37.0	41.0	34.0	41.0
30	38.13757770023106	40.0	37.0	41.0	34.0	41.0
31	38.0891832857	40.0	37.0	41.0	34.0	41.0
32	37.993973462180186	40.0	37.0	41.0	34.0	41.0
33	37.92114446321707	40.0	37.0	41.0	34.0	41.0
34	37.88054717805393	40.0	37.0	41.0	33.0	41.0
35	37.81040143584237	40.0	37.0	41.0	33.0	41.0
36	37.69048543893722	40.0	36.0	41.0	33.0	41.0
37	37.64802176922308	40.0	36.0	41.0	33.0	41.0
38	37.6090624391425	40.0	36.0	41.0	33.0	41.0
39	37.575149610775135	40.0	36.0	41.0	33.0	41.0
40	37.473357439484616	40.0	36.0	41.0	33.0	41.0
41	37.3331891174938	40.0	35.0	41.0	33.0	41.0
42	37.2638876169122	39.0	35.0	41.0	33.0	41.0
43	37.179637144526374	39.0	35.0	41.0	32.0	41.0
44	37.08521787644808	39.0	35.0	41.0	32.0	41.0
45	37.09025911480949	39.0	35.0	41.0	32.0	41.0
46	36.98944382161448	39.0	35.0	41.0	32.0	41.0
47	36.89897733074376	39.0	35.0	41.0	32.0	41.0
48	36.826276757564756	39.0	35.0	41.0	32.0	41.0
49	36.78061612796261	39.0	35.0	41.0	32.0	41.0
50	36.69288026400972	39.0	35.0	41.0	31.0	41.0
51	35.71830014790019	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	17.0
10	24.0
11	26.0
12	14.0
13	22.0
14	15.0
15	35.0
16	50.0
17	80.0
18	136.0
19	263.0
20	492.0
21	737.0
22	1051.0
23	1555.0
24	2206.0
25	3261.0
26	4707.0
27	5915.0
28	6809.0
29	8321.0
30	10555.0
31	13273.0
32	17463.0
33	25384.0
34	50093.0
35	67403.0
36	64069.0
37	103532.0
38	200544.0
39	361689.0
40	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.396440921507637	4.60448542841052	55.06423920881296	16.93483444126889
2	19.727884711542004	4.530798503102746	54.02704310158796	21.714273683767296
3	21.048459680093476	4.690172795839847	51.60716447448064	22.654203049586037
4	19.3254488323254	4.792071286836883	51.28831062197028	24.59416925886743
5	19.208181354049884	6.108119772833736	49.81509845099556	24.868600422120814
6	22.19850205007553	6.521082355665735	52.471091040196214	18.809324554062517
7	85.56978414994236	2.4568273567973553	7.917344323211909	4.05604417004837
8	87.55343617922765	2.8607369745201137	5.721052880895612	3.864773965356618
9	82.02607464485534	5.295984588905907	7.772286347391747	4.90565441884701
10	39.749464453953564	28.320201270573126	16.390498597316743	15.539835678156564
11	29.265078187091103	26.95109819835468	24.742279978736057	19.04154363581816
12	27.862500197375695	22.899791044933234	28.3234645486939	20.914244208997175
13	25.076081750380276	21.685114714752647	30.369118862273876	22.869684672593202
14	21.168779902417455	24.89375924376161	30.589863837088732	23.3475970167322
15	20.08810850926087	25.824635644471112	32.61635955008869	21.470896296179333
16	22.48293358176354	24.785544730595337	31.11504108046086	21.616480607180264
17	22.646097487802184	24.445111135673418	29.311606217071155	23.597185159453243
18	22.824946182227766	25.54473059533772	29.308132404878073	22.322190817556436
19	24.532588042717364	26.621717642228926	27.82228818956488	21.023406125488833
20	27.41300995299827	25.752527724705647	27.757970030474805	19.07649229182128
21	26.795408251882964	25.67926186754249	27.43637923502445	20.0889506455501
22	24.634381266678247	24.25710420910244	28.003136957677388	23.105377566541925
23	24.6344865337144	25.25566731405894	27.70017842762628	22.40966772460038
24	23.97772549514982	23.581289836993992	29.083282015653207	23.357702652202974
25	23.21380261378051	25.623365071344733	27.01036353970936	24.1524687751654
26	22.942529461611745	27.078260778028664	27.54164627117841	22.43756348918118
27	22.498092034969712	26.938571421052355	28.603790665971907	21.959545878006033
28	21.479949261288574	26.527503644871125	29.27802603253804	22.71452106130226
29	22.688730637444536	25.09071386840568	28.930750080266115	23.28980541388367
30	23.356334180732972	25.517045364829233	28.102298505734424	23.024321948703374
31	23.890669656250495	26.21549214971078	26.68035138136668	23.213486812672045
32	24.575642260504335	26.894464532903843	26.55382040390962	21.976072802682204
33	24.34868653055639	26.394551378208668	26.76824935655524	22.4885127346797
34	23.990673340596757	26.010642497355164	27.4245893269752	22.574094835072874
35	23.44581116146384	24.984604695962485	27.48448627054681	24.085097872026864
36	23.204960182743577	27.864395004026466	27.065523466654035	21.86512134657593
37	23.815508992436563	26.648771270520495	28.110930402699047	21.424789334343895
38	22.761996494607693	26.302863789718568	27.410273010058265	23.52486670561547
39	23.109482980951928	25.908112404141203	27.47732811208834	23.505076502818525
40	24.100466859305342	25.322511882016705	28.81821961861753	21.758801640060422
41	21.8683846246967	25.88432205397041	29.194759806940258	23.052533514392636
42	22.792734469164653	25.634944445321672	28.086718984383634	23.48560210113004
43	22.876421762907054	25.740316748511788	27.68417783813088	23.69908365045028
44	22.78999752622465	24.837546646455397	27.818077508118723	24.554378319201234
45	23.516129541614692	24.51574531693273	26.56076802829578	25.407357113156802
46	22.811787802708523	25.563573394809282	27.5652260872769	24.059412715205298
47	21.982388824851444	25.14945287457959	29.824993552394037	23.04316474817493
48	22.16881674588011	23.72645307985031	29.887311637797183	24.217418536472398
49	22.735890269641512	24.373845352197186	29.22760312222029	23.66266125594101
50	22.124920391803908	24.682698836272916	28.078087087419014	25.11429368450417
51	21.368997805182296	24.227839973051637	27.837867710915663	26.5652945108504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	590.0
2	1042.0
3	1999.5
4	2957.0
5	2105.0
6	1253.0
7	1326.0
8	1399.0
9	1393.5
10	1388.0
11	1376.0
12	1364.0
13	1336.5
14	1309.0
15	1380.0
16	1451.0
17	1447.5
18	1444.0
19	1613.0
20	1782.0
21	2151.0
22	2520.0
23	3240.5
24	3961.0
25	4689.0
26	7161.0
27	8905.0
28	10800.5
29	12696.0
30	14515.0
31	16334.0
32	18419.0
33	20504.0
34	24011.5
35	27519.0
36	30019.0
37	32519.0
38	35043.0
39	37567.0
40	41232.5
41	44898.0
42	48454.0
43	52010.0
44	56657.5
45	61305.0
46	67706.0
47	74107.0
48	81132.5
49	88158.0
50	87519.5
51	86881.0
52	79379.0
53	71877.0
54	64780.0
55	57683.0
56	53974.5
57	50266.0
58	49215.0
59	48164.0
60	44143.0
61	40122.0
62	35917.0
63	31712.0
64	26697.5
65	21683.0
66	17578.5
67	13474.0
68	11177.5
69	8881.0
70	7564.5
71	6248.0
72	5213.5
73	4179.0
74	3079.5
75	1607.0
76	1234.0
77	1106.5
78	979.0
79	647.0
80	315.0
81	247.0
82	179.0
83	136.0
84	93.0
85	62.5
86	32.0
87	29.5
88	27.0
89	17.0
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	949965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.83345963261455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.76721740718742	20.692971660552107
2	10.300636949460975	5.940059993450018
3	3.6172007416136536	3.1288923469914214
4	1.802574316492905	2.0789781515754373
5	1.176725378177848	1.6964531845182034
6	0.8295218494831419	1.4350790856866362
7	0.6563779207796495	1.3247952397777647
8	0.5731092285293298	1.3219777444703438
9	0.4989713170576485	1.29483623953928
>10	8.586437737305683	55.537409543480955
>50	0.15689487126092042	2.8527752428429176
>100	0.031378968014272	1.537673381447625
>500	0.001107492988738956	0.18159149859819804
>1k	0.0018458216478982599	0.9765066870690907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	2670	0.2810629865310827	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2159	0.22727153105640735	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1574	0.16569031490633865	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.15074239577247583	No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1339	0.14095256141015722	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0526703615396356E-4	0.0	0.0	0.24137731390103845	0.0
2	1.0526703615396356E-4	0.0	0.0	0.878453416704826	0.0
3	1.0526703615396356E-4	0.0	0.0	1.2462564410267747	0.0
4	1.0526703615396356E-4	0.0	0.0	1.6611138305095452	0.0
5	1.0526703615396356E-4	0.0	0.0	2.333349123388756	0.0
6	1.0526703615396356E-4	0.0	0.0	2.854420952350876	0.0
7	1.0526703615396356E-4	0.0	0.0	3.322332928055244	0.0
8	1.0526703615396356E-4	0.0	0.0	4.069413083639923	0.0
9	1.0526703615396356E-4	0.0	0.0	4.453532498565736	0.0
10	1.0526703615396356E-4	0.0	0.0	5.124820388119562	0.0
11	2.1053407230792713E-4	0.0	0.0	6.389288026400973	0.0
12	2.1053407230792713E-4	0.0	0.0	7.58354255156769	0.0
13	2.1053407230792713E-4	0.0	0.0	7.937029258972699	0.0
14	2.1053407230792713E-4	0.0	0.0	8.069981525635155	0.0
15	2.1053407230792713E-4	0.0	0.0	8.272410036159227	0.0
16	2.1053407230792713E-4	0.0	0.0	8.757480538756692	0.0
17	2.1053407230792713E-4	0.0	0.0	9.351081355628892	0.0
18	2.1053407230792713E-4	0.0	0.0	9.996894622433459	0.0
19	2.1053407230792713E-4	0.0	0.0	10.517966451395578	0.0
20	2.1053407230792713E-4	0.0	0.0	11.062618096456186	0.0
21	2.1053407230792713E-4	0.0	0.0	11.86054223050323	0.0
22	2.1053407230792713E-4	0.0	0.0	12.716994836651876	0.0
23	2.1053407230792713E-4	0.0	0.0	13.490918086455817	0.0
24	2.1053407230792713E-4	0.0	0.0	14.075571205254931	0.0
25	2.1053407230792713E-4	0.0	0.0	14.583589921733958	0.0
26	2.1053407230792713E-4	0.0	0.0	15.037711915702157	0.0
27	2.1053407230792713E-4	0.0	0.0	15.463411809908786	0.0
28	2.1053407230792713E-4	0.0	0.0	15.925849899733148	0.0
29	2.1053407230792713E-4	0.0	0.0	16.425341986283705	0.0
30	2.1053407230792713E-4	0.0	0.0	16.97862552830894	0.0
31	2.1053407230792713E-4	0.0	0.0	17.525382514092623	0.0
32	2.1053407230792713E-4	0.0	0.0	18.056770512597833	0.0
33	2.1053407230792713E-4	0.0	0.0	18.55573626396762	0.0
34	2.1053407230792713E-4	0.0	0.0	19.057754759385872	0.0
35	2.1053407230792713E-4	0.0	0.0	19.546614875284877	0.0
36	2.1053407230792713E-4	0.0	0.0	20.069160442753155	0.0
37	3.158011084618907E-4	0.0	0.0	20.59117967504066	0.0
38	3.158011084618907E-4	0.0	0.0	21.092250767133525	0.0
39	4.2106814461585426E-4	0.0	0.0	21.615954271999495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTG	20	7.0326694E-4	45.0	1
TCGTTTG	420	0.0	42.32143	1
TTTTGCG	710	0.0	40.880283	1
CGGGCGA	300	0.0	40.500004	6
TATTGCG	45	1.927765E-8	40.0	1
CGTTTGG	800	0.0	39.375	2
GGGCGAT	1535	0.0	39.136806	7
TGGGCGA	1070	0.0	39.11215	6
TTTTTCG	440	0.0	38.863636	1
CATACGA	265	0.0	38.207546	18
TTTCTCG	320	0.0	37.96875	1
TTGGGAT	2585	0.0	37.77563	5
TTGGGAC	1820	0.0	37.706043	5
TTTGGGC	2270	0.0	37.566082	4
GGTACGC	30	1.13977265E-4	37.500004	8
CAACGCG	30	1.13977265E-4	37.500004	17
AAGCGTA	30	1.13977265E-4	37.500004	25
TACGGGT	30	1.13977265E-4	37.500004	4
ATCGACG	30	1.13977265E-4	37.500004	16
GCGATGT	235	0.0	37.340427	9
>>END_MODULE