FastQCFastQC Report
Sat 14 Jan 2017
SRR2933870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414170
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC60290.426327810659256Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT55580.3930220553398814Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC52150.3687675456274705Illumina PCR Primer Index 10 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC46280.32725909897678496Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC23100.16334669806317487No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC21710.15351761103686262No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.1490627010896851No Hit
TCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG19820.14015288119533012Illumina PCR Primer Index 10 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG19580.13845577264402442No Hit
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG15100.1067764130196511Illumina PCR Primer Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA453.8562575E-1045.00000422
CGCATAG351.2121927E-745.00000424
TATCTCG502.1827873E-1145.0000041
TCGAACA253.891262E-545.00000418
CGTATTA207.033839E-445.025
CCCTCGT207.033839E-445.014
TAACGCC207.033839E-445.031
TTAACGG207.033839E-445.02
CTATTCG207.033839E-445.01
ATAGTCG302.165858E-644.99999627
TATTGCG1000.042.7500041
TTCCGCA556.184564E-1140.9090921
CGATAAC556.184564E-1140.90909216
TTCTTCG4050.040.01
CGAATAT1300.039.8076914
GCGATCG403.458972E-739.3759
TACGGGA2700.039.1666644
TTTCGCG3700.038.918921
TACGGGT356.249309E-638.571434
TCTTGCG4150.038.4939731
Produced by FastQC (version 0.11.5)