##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933870.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1414170
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43258448418507	33.0	31.0	34.0	31.0	34.0
2	32.6561841928481	34.0	31.0	34.0	31.0	34.0
3	32.71373809372282	34.0	31.0	34.0	31.0	34.0
4	36.08283869690349	37.0	35.0	37.0	35.0	37.0
5	36.11281882659086	37.0	35.0	37.0	35.0	37.0
6	35.70327329811833	37.0	35.0	37.0	35.0	37.0
7	36.043830656851725	37.0	35.0	37.0	35.0	37.0
8	36.05105821789459	37.0	36.0	37.0	35.0	37.0
9	37.900175367883634	39.0	38.0	39.0	35.0	39.0
10	37.57347490047165	39.0	37.0	39.0	35.0	39.0
11	37.51300126576013	39.0	37.0	39.0	35.0	39.0
12	37.387945579385786	39.0	37.0	39.0	35.0	39.0
13	37.33685412644873	39.0	37.0	39.0	35.0	39.0
14	38.593323999236304	40.0	38.0	41.0	35.0	41.0
15	38.64618115219528	40.0	38.0	41.0	35.0	41.0
16	38.708107936103865	40.0	38.0	41.0	35.0	41.0
17	38.66710437924719	40.0	38.0	41.0	35.0	41.0
18	38.61424510490252	40.0	38.0	41.0	35.0	41.0
19	38.64279612776399	40.0	38.0	41.0	35.0	41.0
20	38.58824469476796	40.0	38.0	41.0	35.0	41.0
21	38.510420246505014	40.0	38.0	41.0	34.0	41.0
22	38.5002220383688	40.0	38.0	41.0	34.0	41.0
23	38.442390943097365	40.0	38.0	41.0	34.0	41.0
24	38.40000919267132	40.0	37.0	41.0	34.0	41.0
25	38.319735958194556	40.0	37.0	41.0	34.0	41.0
26	38.23638317882574	40.0	37.0	41.0	34.0	41.0
27	38.22866416343155	40.0	37.0	41.0	34.0	41.0
28	38.14420897063295	40.0	37.0	41.0	34.0	41.0
29	38.14103254912776	40.0	37.0	41.0	34.0	41.0
30	38.0860950239363	40.0	37.0	41.0	34.0	41.0
31	38.01950331289732	40.0	37.0	41.0	34.0	41.0
32	37.94881520609262	40.0	37.0	41.0	34.0	41.0
33	37.88613603739296	40.0	37.0	41.0	34.0	41.0
34	37.834534744761946	40.0	37.0	41.0	33.0	41.0
35	37.80656922435068	40.0	37.0	41.0	33.0	41.0
36	37.71693360769922	40.0	36.0	41.0	33.0	41.0
37	37.6366327952085	40.0	36.0	41.0	33.0	41.0
38	37.58616927243542	40.0	36.0	41.0	33.0	41.0
39	37.55691960655367	40.0	36.0	41.0	33.0	41.0
40	37.427872179440946	40.0	36.0	41.0	33.0	41.0
41	37.3024798998706	40.0	35.0	41.0	33.0	41.0
42	37.288147111026255	39.0	35.0	41.0	33.0	41.0
43	37.19315640976686	39.0	35.0	41.0	32.0	41.0
44	37.090219704844536	39.0	35.0	41.0	32.0	41.0
45	37.09115099316207	39.0	35.0	41.0	32.0	41.0
46	36.98077529575652	39.0	35.0	41.0	32.0	41.0
47	36.89667720288226	39.0	35.0	41.0	32.0	41.0
48	36.84680696097357	39.0	35.0	41.0	32.0	41.0
49	36.82705261743638	39.0	35.0	41.0	32.0	41.0
50	36.743375973185685	39.0	35.0	41.0	32.0	41.0
51	35.78280616898959	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	25.0
10	44.0
11	29.0
12	17.0
13	23.0
14	17.0
15	26.0
16	52.0
17	100.0
18	179.0
19	334.0
20	587.0
21	1017.0
22	1604.0
23	2296.0
24	3463.0
25	5063.0
26	6892.0
27	8673.0
28	10511.0
29	12632.0
30	15945.0
31	20539.0
32	27301.0
33	39095.0
34	74431.0
35	101738.0
36	98638.0
37	159195.0
38	307985.0
39	515394.0
40	316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.384904219436134	4.570171902953676	54.31793914451587	16.726984733094323
2	19.337703387852944	5.178585318596774	52.961312996315854	22.52239829723442
3	21.076037534384128	5.2144367367431075	51.160610110524196	22.548915618348573
4	19.82675350205421	4.650572420571784	50.97138250705361	24.551291570320398
5	19.32207584660967	6.094033956313597	48.85084537219712	25.733044824879613
6	21.91815694011328	7.407454549311611	51.86285948648324	18.811529024091868
7	82.95806020492586	2.597636776342307	9.671326643897126	4.772976374834709
8	84.04583607345651	3.6468034253307593	7.208609997383625	5.098750503829101
9	78.11486596378087	5.577900818147747	9.739918114512399	6.567315103558978
10	38.53886025018208	27.452852203059035	18.093298542608032	15.914989004150845
11	29.15314283289845	27.033737103742833	24.644137550648082	19.168982512710635
12	28.004553907946004	21.901327280312834	29.555286846701602	20.538831965039563
13	25.329203702525156	22.517943387287243	30.368555407058555	21.784297503129043
14	20.27196164534674	26.101812370507083	29.77103177128634	23.85519421285984
15	18.278495513269267	26.2288833732861	34.10721483272874	21.385406280715895
16	20.587199558751777	24.251681198158636	32.96562648055043	22.195492762539157
17	20.364171209967687	24.33582949716088	29.863736325901414	25.436262966970023
18	20.867929598280263	25.448637716823296	30.31707644766895	23.36635623722749
19	23.733850951441482	26.25129934873459	28.067841914338448	21.94700778548548
20	26.463367204791506	25.884299624514735	28.182113890126363	19.4702192805674
21	25.42155469285871	26.13342101727515	27.546900301943893	20.898123987922244
22	22.99051740596958	25.617217166253	26.737238097258466	24.655027330518962
23	22.975526280433044	26.343155349074017	27.06647715621177	23.614841214281167
24	23.306957437931793	23.504812009871515	28.86032089494191	24.327909657254786
25	22.3823868417517	25.607388079226684	26.980348897233007	25.029876181788612
26	20.745949921155166	26.779524385328497	27.529646365005622	24.944879328510716
27	21.472736658251836	27.103177128633753	27.55913362608456	23.864952587029848
28	19.09409759788427	27.41339442924118	29.541922116860064	23.95058585601448
29	20.291690532255668	25.060919125706242	29.611574280319903	25.03581606171818
30	21.032478414900613	25.203759095441143	28.93697363117588	24.82678885848236
31	20.545973963526308	28.548547911495785	26.56201163933615	24.343466485641756
32	22.724707779121324	27.40165609509465	26.22690341330957	23.646732712474456
33	22.207796799536123	26.48578318024	26.5307565568496	24.775663463374276
34	20.952219322995113	26.340609686247056	27.699710784417714	25.007460206340117
35	21.023851446431475	24.027167879392152	27.28639413931847	27.6625865348579
36	20.620222462645934	26.583791199077904	26.280008768394183	26.515977569881983
37	20.5378419850513	28.110269628121088	28.094429948308903	23.257458438518707
38	20.972655338467085	26.623036834326847	28.402384437514584	24.00192338969148
39	21.20134071575553	24.68953520439551	30.38142514690596	23.727698932943
40	22.46540373505307	24.101557804224385	30.61746466125006	22.81557379947248
41	20.078915547635713	25.737782586252006	28.009433095031007	26.17386877108127
42	21.4496842670966	25.160907104520675	26.460185126257805	26.92922350212492
43	21.456119137020302	24.656795151926573	27.678638353239005	26.208447357814123
44	20.951441481575767	24.50504536229732	27.99712905803404	26.546384098092872
45	21.2008457257614	22.96385865914282	27.600571359878938	28.234724255216843
46	20.612726899877668	24.91440208744352	28.315902614254295	26.156968398424517
47	19.506141411570038	25.186575871359175	29.899729169760352	25.407553547310435
48	19.733695383157613	23.294016985228083	30.112009164386176	26.860278467228127
49	19.91203320675732	23.346061647468126	31.455836285595083	25.286068860179466
50	19.908002573947968	23.03400581259679	30.197218156232985	26.86077345722226
51	18.997503836172456	23.13201383143469	28.086439395546503	29.78404293684635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	770.0
2	1322.0
3	2777.0
4	4232.0
5	2995.5
6	1759.0
7	1868.0
8	1977.0
9	1961.0
10	1945.0
11	1993.0
12	2041.0
13	1899.5
14	1758.0
15	1850.5
16	1943.0
17	2129.0
18	2315.0
19	2642.5
20	2970.0
21	3570.5
22	4171.0
23	4487.5
24	4804.0
25	6987.5
26	10706.5
27	12242.0
28	15236.0
29	18230.0
30	19889.5
31	21549.0
32	26076.5
33	30604.0
34	35080.5
35	39557.0
36	42542.5
37	45528.0
38	49588.0
39	53648.0
40	59972.5
41	66297.0
42	72936.0
43	79575.0
44	88122.0
45	96669.0
46	112114.0
47	127559.0
48	138932.5
49	150306.0
50	146019.0
51	141732.0
52	122597.0
53	103462.0
54	90106.5
55	76751.0
56	71902.5
57	67054.0
58	66917.0
59	66780.0
60	62639.5
61	58499.0
62	51393.0
63	44287.0
64	35649.5
65	27012.0
66	21748.5
67	16485.0
68	13746.0
69	11007.0
70	8992.0
71	6977.0
72	6096.0
73	5215.0
74	4324.5
75	2549.5
76	1665.0
77	1216.0
78	767.0
79	514.0
80	261.0
81	262.5
82	264.0
83	152.0
84	40.0
85	27.0
86	14.0
87	12.5
88	11.0
89	22.5
90	34.0
91	30.5
92	27.0
93	14.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1414170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.96554278614287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.31574767208255	18.691367596050252
2	12.401188721485466	6.6880957013649756
3	5.095256455046142	4.121890678347522
4	2.5428701800714517	2.7427949856129423
5	1.4760998142607746	1.990191634903323
6	0.9514573529243564	1.5393938375683183
7	0.6815486091492152	1.286482972659453
8	0.49460079434252874	1.0669743105522969
9	0.4061628676756955	0.9857161967806307
>10	5.750864558390626	40.4031066282574
>50	0.7900885385506689	12.91298066356952
>100	0.08722154777215584	4.3288518363031265
>500	0.003446444124275302	0.5787005687787882
>1k	0.0026511108648271556	1.4623002018987168
>5k	7.953332594481466E-4	1.201152187352835
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	6029	0.426327810659256	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	5558	0.3930220553398814	Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	5215	0.3687675456274705	Illumina PCR Primer Index 10 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	4628	0.32725909897678496	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	2310	0.16334669806317487	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	2171	0.15351761103686262	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2108	0.1490627010896851	No Hit
TCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1982	0.14015288119533012	Illumina PCR Primer Index 10 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	1958	0.13845577264402442	No Hit
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1510	0.1067764130196511	Illumina PCR Primer Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.828514252176188E-4	0.0	0.0	0.4134580708118543	0.0
2	2.828514252176188E-4	0.0	0.0	1.627597813558483	0.0
3	2.828514252176188E-4	0.0	0.0	2.342575503652319	0.0
4	2.828514252176188E-4	0.0	0.0	3.0763628135231267	0.0
5	2.828514252176188E-4	0.0	0.0	4.37726723095526	0.0
6	2.828514252176188E-4	0.0	0.0	5.167978390151113	0.0
7	2.828514252176188E-4	0.0	0.0	5.886916000197996	0.0
8	2.828514252176188E-4	0.0	0.0	6.985723074312141	0.0
9	2.828514252176188E-4	0.0	0.0	7.5061696967125595	0.0
10	2.828514252176188E-4	0.0	0.0	8.56523614558363	0.0
11	2.828514252176188E-4	0.0	0.0	10.298478966460893	0.0
12	2.828514252176188E-4	0.0	0.0	11.998698883444	0.0
13	2.828514252176188E-4	0.0	0.0	12.55160270688814	0.0
14	2.828514252176188E-4	0.0	0.0	12.756457851602	0.0
15	2.828514252176188E-4	0.0	0.0	13.093828889030314	0.0
16	2.828514252176188E-4	0.0	0.0	13.798482502103708	0.0
17	2.828514252176188E-4	0.0	0.0	14.741721292348162	0.0
18	2.828514252176188E-4	0.0	0.0	15.720387223601122	0.0
19	2.828514252176188E-4	0.0	0.0	16.447527524979318	0.0
20	2.828514252176188E-4	0.0	0.0	17.200902296046443	0.0
21	2.828514252176188E-4	0.0	0.0	18.232037166677273	0.0
22	2.828514252176188E-4	0.0	0.0	19.373342667430364	0.0
23	2.828514252176188E-4	0.0	0.0	20.442662480465575	0.0
24	2.828514252176188E-4	0.0	0.0	21.25494106083427	0.0
25	2.828514252176188E-4	0.0	0.0	21.97274726518028	0.0
26	2.828514252176188E-4	0.0	0.0	22.595373964940567	0.0
27	3.535642815220235E-4	0.0	0.0	23.200393163481053	0.0
28	3.535642815220235E-4	0.0	0.0	23.839566671616566	0.0
29	3.535642815220235E-4	0.0	0.0	24.512045935071455	0.0
30	3.535642815220235E-4	0.0	0.0	25.2480253434877	0.0
31	3.535642815220235E-4	0.0	0.0	25.961376637886534	0.0
32	3.535642815220235E-4	0.0	0.0	26.64729134403926	0.0
33	3.535642815220235E-4	0.0	0.0	27.307890847635008	0.0
34	3.535642815220235E-4	0.0	0.0	27.947276494339437	0.0
35	3.535642815220235E-4	0.0	0.0	28.60957310648649	0.0
36	3.535642815220235E-4	0.0	0.0	29.29089147697943	0.0
37	3.535642815220235E-4	0.0	0.0	29.968037788950408	0.0
38	3.535642815220235E-4	0.0	0.0	30.60721129708592	0.0
39	3.535642815220235E-4	0.0	0.0	31.254233932271227	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	45	3.8562575E-10	45.000004	22
CGCATAG	35	1.2121927E-7	45.000004	24
TATCTCG	50	2.1827873E-11	45.000004	1
TCGAACA	25	3.891262E-5	45.000004	18
CGTATTA	20	7.033839E-4	45.0	25
CCCTCGT	20	7.033839E-4	45.0	14
TAACGCC	20	7.033839E-4	45.0	31
TTAACGG	20	7.033839E-4	45.0	2
CTATTCG	20	7.033839E-4	45.0	1
ATAGTCG	30	2.165858E-6	44.999996	27
TATTGCG	100	0.0	42.750004	1
TTCCGCA	55	6.184564E-11	40.909092	1
CGATAAC	55	6.184564E-11	40.909092	16
TTCTTCG	405	0.0	40.0	1
CGAATAT	130	0.0	39.80769	14
GCGATCG	40	3.458972E-7	39.375	9
TACGGGA	270	0.0	39.166664	4
TTTCGCG	370	0.0	38.91892	1
TACGGGT	35	6.249309E-6	38.57143	4
TCTTGCG	415	0.0	38.493973	1
>>END_MODULE