SRR2933868.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933868.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1020669
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3216	0.3150874573441537	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3063	0.30009728913095235	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	3063	0.30009728913095235	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2752	0.2696270779263405	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.19800738535215626	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1539	0.15078345673278998	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1208	0.11835374641534131	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	1204	0.11796184659277396	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	3.8904975E-5	45.0	8
ACCGATA	20	7.032917E-4	45.0	10
GCGATCG	20	7.032917E-4	45.0	9
AACCCGA	55	6.184564E-11	40.909092	24
CATACGA	140	0.0	38.57143	18
TCTTGCG	205	0.0	38.414635	1
TACGGGA	130	0.0	38.07692	4
GTTTGCG	225	0.0	38.0	1
TTGGGCG	925	0.0	37.945946	5
TTATGGG	640	0.0	37.617188	3
TTTGGGC	2125	0.0	37.588234	4
GAATGCG	30	1.13983246E-4	37.499996	41
CCGATCA	30	1.13983246E-4	37.499996	10
CGAATAT	60	1.546141E-10	37.499996	14
TTACGCG	30	1.13983246E-4	37.499996	1
GACCGAT	465	0.0	37.258064	9
CGGGATA	260	0.0	37.211536	6
TGGGCGA	1205	0.0	37.157677	6
TTTGGGA	4580	0.0	36.94323	4
TTTCGCG	295	0.0	36.61017	1