##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933868.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1020669
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.439638119703844	33.0	31.0	34.0	31.0	34.0
2	32.65666538319475	34.0	31.0	34.0	31.0	34.0
3	32.7198082826068	34.0	31.0	34.0	31.0	34.0
4	36.086036707296884	37.0	35.0	37.0	35.0	37.0
5	36.105071281678974	37.0	35.0	37.0	35.0	37.0
6	35.727118194047236	37.0	35.0	37.0	35.0	37.0
7	36.053114182952555	37.0	35.0	37.0	35.0	37.0
8	36.07046260834805	37.0	36.0	37.0	35.0	37.0
9	37.905289569880146	39.0	38.0	39.0	35.0	39.0
10	37.59528407348514	39.0	37.0	39.0	35.0	39.0
11	37.52821629735007	39.0	37.0	39.0	35.0	39.0
12	37.21194628229132	39.0	37.0	39.0	34.0	39.0
13	37.042122372679096	39.0	37.0	39.0	33.0	39.0
14	37.88624421825293	40.0	37.0	41.0	33.0	41.0
15	38.07113765579243	40.0	37.0	41.0	33.0	41.0
16	38.276970300851694	40.0	37.0	41.0	33.0	41.0
17	38.306570494450206	40.0	37.0	41.0	34.0	41.0
18	38.287141080996875	40.0	37.0	41.0	34.0	41.0
19	38.2938533452079	40.0	37.0	41.0	34.0	41.0
20	38.19772227823124	40.0	37.0	41.0	34.0	41.0
21	38.11844976187187	40.0	36.0	41.0	34.0	41.0
22	38.17688692416444	40.0	36.0	41.0	34.0	41.0
23	38.157158687096405	40.0	36.0	41.0	34.0	41.0
24	38.12028679229015	40.0	36.0	41.0	34.0	41.0
25	38.020320005800116	40.0	36.0	41.0	34.0	41.0
26	37.944504045875796	40.0	36.0	41.0	34.0	41.0
27	37.94064383262351	40.0	36.0	41.0	34.0	41.0
28	37.86312898696835	40.0	36.0	41.0	34.0	41.0
29	37.820862591104465	40.0	36.0	41.0	34.0	41.0
30	37.74864231205219	40.0	36.0	41.0	34.0	41.0
31	37.629493988746596	40.0	35.0	41.0	33.0	41.0
32	37.562923925386194	40.0	35.0	41.0	33.0	41.0
33	37.52486947286535	40.0	35.0	41.0	33.0	41.0
34	37.444358553066664	40.0	35.0	41.0	33.0	41.0
35	37.376616709236785	40.0	35.0	41.0	33.0	41.0
36	37.320540743375176	40.0	35.0	41.0	32.0	41.0
37	37.23641552746287	40.0	35.0	41.0	32.0	41.0
38	37.18195712811891	39.0	35.0	41.0	32.0	41.0
39	37.147041793176825	39.0	35.0	41.0	32.0	41.0
40	37.032300383376004	39.0	35.0	41.0	32.0	41.0
41	36.877114911886224	39.0	35.0	41.0	32.0	41.0
42	36.87853750824214	39.0	35.0	41.0	31.0	41.0
43	36.81727572797841	39.0	35.0	41.0	32.0	41.0
44	36.72831740750429	39.0	35.0	41.0	31.0	41.0
45	36.76163575066941	39.0	35.0	41.0	32.0	41.0
46	36.714877203089344	39.0	35.0	41.0	32.0	41.0
47	36.64233752568168	39.0	35.0	41.0	31.0	41.0
48	36.57508163763179	39.0	35.0	41.0	31.0	41.0
49	36.573901039416306	39.0	35.0	41.0	31.0	41.0
50	36.49020593355927	39.0	35.0	41.0	31.0	41.0
51	35.53234202273215	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	12.0
10	18.0
11	20.0
12	11.0
13	7.0
14	23.0
15	38.0
16	67.0
17	114.0
18	200.0
19	332.0
20	614.0
21	960.0
22	1396.0
23	2063.0
24	2952.0
25	4280.0
26	5926.0
27	7341.0
28	8779.0
29	10479.0
30	12749.0
31	17144.0
32	23668.0
33	36963.0
34	75894.0
35	74937.0
36	68657.0
37	108128.0
38	207071.0
39	349612.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.64403249241429	4.459232131082653	55.55268162352339	15.344053752979663
2	20.77901846729939	4.376639243476582	52.43952740800396	22.404814881220062
3	21.297893832378566	4.602177591364096	50.83989030723966	23.260038269017674
4	19.72196667087959	4.9604720041463	50.13172732786045	25.18583399711366
5	19.292150540478843	5.90671412573518	49.615007411805394	25.186127921980585
6	21.84674953388415	6.806908018172394	52.85552906965921	18.490813378284244
7	83.12959441307612	2.5335343779423103	9.382963526863264	4.953907682118297
8	84.79644233341074	2.97148242966133	7.420231240490306	4.811843996437631
9	78.67663267915455	5.424873293888616	10.134823336458734	5.7636706904980946
10	41.58635169677927	25.853533319812787	18.03356426030378	14.526550723104158
11	33.136305697537594	22.921828722142045	25.593703737450635	18.348161842869725
12	29.883439195272903	20.66487764397665	29.12766038745176	20.32402277329869
13	27.917963610141978	20.941264993842275	31.70910451870293	19.43166687731282
14	21.268795270552943	22.714219791136987	34.88329713158723	21.133687806722847
15	19.68414833800184	22.8467799061204	37.17385361953777	20.295218136339987
16	25.69177666804811	22.35896260197968	30.75864947402145	21.190611255950753
17	25.62652534759065	23.038516894311474	28.634454460750742	22.700503297347133
18	26.019110994847498	22.442829164009094	30.73082458661917	20.80723525452424
19	25.996380805138593	24.79687342321556	28.274984348500837	20.931761423145016
20	24.511668327342164	28.915054733708967	27.8395836456285	18.73369329332036
21	24.0370776422131	26.22289890258252	31.29721780518464	18.442805650019743
22	22.410105528824722	23.399064731073445	32.1296130283177	22.061216711784134
23	21.131434382743084	24.64138716861196	31.72634811089589	22.500830337749065
24	22.611835962491266	26.064865299132233	28.805028858523187	22.51826987985331
25	24.192367946905414	25.129106497797032	26.31078243779325	24.367743117504304
26	20.40994680939658	25.166630905807857	27.97684655848272	26.446575726312837
27	20.21105764944365	23.75108874669457	31.752213499185338	24.285640104676443
28	18.823634302599572	24.875939212418523	29.268254448797798	27.03217203618411
29	20.504590616546597	26.595301708977154	27.842620869253405	25.05748680522285
30	24.001904633137677	23.5970721164256	29.23268953989981	23.168333710536913
31	22.835414811265945	24.544293987570896	29.355942034097243	23.264349167065916
32	24.357553722117554	25.142235141853043	26.197327439160002	24.302883696869408
33	24.247625821887407	23.78880910461668	26.63694106512493	25.326624008370977
34	19.670627794123266	24.13044777493977	30.671941638278422	25.526982792658544
35	19.017820664681693	27.272308652462257	27.680864217488725	26.02900646536732
36	21.639336552790375	26.190959067043284	26.997586876842544	25.172117503323797
37	19.472914333638037	27.898172669102323	28.2934036401615	24.33550935709814
38	21.38176039440798	27.432889604759232	26.476262137872318	24.70908786296047
39	20.20576700183899	25.132535621244497	26.933217330985855	27.72848004593066
40	22.507100734910143	23.317941467802	29.280403343297383	24.894554453990473
41	19.559622169381065	24.15543138862844	28.27400459894442	28.010941843046083
42	19.47154268425905	24.023263173467598	29.616261491237612	26.88893265103574
43	19.96474861096007	22.865885022470557	30.252608828131354	26.916757538438024
44	20.89844993822679	22.839137859580333	28.537361279709682	27.725050922483195
45	20.124251838744982	22.502397937039333	27.26486255583348	30.108487668382207
46	20.12062676538623	23.31480626922146	29.382003372297973	27.182563593094333
47	17.503715700192718	24.811569666561834	31.962369779037083	25.722344854208366
48	18.604758251695703	25.893801026581585	29.559142092098416	25.942298629624293
49	21.536168924499517	22.685807054000858	30.108389693426567	25.66963432807306
50	19.680523264643092	23.6132379841065	28.57625733709949	28.12998141415092
51	18.442511725152816	23.7399196017514	27.588865734141038	30.228702938954743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	543.5
2	982.0
3	2294.0
4	3606.0
5	2482.0
6	1358.0
7	1387.0
8	1416.0
9	1480.0
10	1544.0
11	1549.0
12	1554.0
13	1543.5
14	1533.0
15	1502.5
16	1472.0
17	1571.0
18	1670.0
19	1775.0
20	1880.0
21	2072.5
22	2265.0
23	2997.0
24	3729.0
25	4643.0
26	7242.5
27	8928.0
28	10111.0
29	11294.0
30	13226.5
31	15159.0
32	18428.5
33	21698.0
34	23453.0
35	25208.0
36	27818.5
37	30429.0
38	33581.0
39	36733.0
40	40477.5
41	44222.0
42	49330.0
43	54438.0
44	59698.5
45	64959.0
46	75060.5
47	85162.0
48	98893.0
49	112624.0
50	111721.0
51	110818.0
52	96683.5
53	82549.0
54	74635.5
55	66722.0
56	61204.0
57	55686.0
58	51619.0
59	47552.0
60	41191.0
61	34830.0
62	30953.0
63	27076.0
64	22261.5
65	17447.0
66	15249.0
67	13051.0
68	10796.0
69	8541.0
70	7576.0
71	6611.0
72	5686.5
73	4762.0
74	3710.5
75	2037.5
76	1416.0
77	1121.5
78	827.0
79	571.0
80	315.0
81	223.5
82	132.0
83	109.0
84	86.0
85	49.0
86	12.0
87	27.0
88	42.0
89	24.5
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1020669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.665337675009198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40841306723327	21.897630995432475
2	11.251151162747945	6.900406992764763
3	4.211396150631487	3.874316551270454
4	2.0899321995476825	2.563539064680176
5	1.2727431041659278	1.9514548531393794
6	0.9237634357782828	1.6996510613980629
7	0.6975522567978547	1.4973472850469662
8	0.5389653640261503	1.3222043906396923
9	0.4781280640841033	1.3195762683333718
>10	6.875788388029032	47.73400113323941
>50	0.18492242962334504	3.594058327061462
>100	0.06271831347586437	3.4076723541354874
>500	0.0019397416538861174	0.44711262668721785
>1k	0.0025863222051814897	1.791028096171015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3216	0.3150874573441537	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3063	0.30009728913095235	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	3063	0.30009728913095235	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2752	0.2696270779263405	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.19800738535215626	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1539	0.15078345673278998	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1208	0.11835374641534131	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	1204	0.11796184659277396	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.939248669255165E-4	0.0	0.0	0.3145975825659445	0.0
2	2.939248669255165E-4	0.0	0.0	1.2686777006061711	0.0
3	2.939248669255165E-4	0.0	0.0	1.860838332505445	0.0
4	2.939248669255165E-4	0.0	0.0	2.4867023491455114	0.0
5	2.939248669255165E-4	0.0	0.0	3.567758009697561	0.0
6	2.939248669255165E-4	0.0	0.0	4.480492696456932	0.0
7	2.939248669255165E-4	0.0	0.0	5.286140756699773	0.0
8	2.939248669255165E-4	0.0	0.0	6.641820217915896	0.0
9	2.939248669255165E-4	0.0	0.0	7.45618804921086	0.0
10	2.939248669255165E-4	0.0	0.0	8.686067667382863	0.0
11	2.939248669255165E-4	0.0	0.0	10.519081112486026	0.0
12	2.939248669255165E-4	0.0	0.0	12.056308166506478	0.0
13	2.939248669255165E-4	0.0	0.0	12.603400318810506	0.0
14	2.939248669255165E-4	0.0	0.0	12.84872960773767	0.0
15	2.939248669255165E-4	0.0	0.0	13.149512721558116	0.0
16	2.939248669255165E-4	0.0	0.0	13.755389847247246	0.0
17	2.939248669255165E-4	0.0	0.0	14.54790926343408	0.0
18	2.939248669255165E-4	0.0	0.0	15.404308350699393	0.0
19	2.939248669255165E-4	0.0	0.0	15.999506206223565	0.0
20	2.939248669255165E-4	0.0	0.0	16.71227400851794	0.0
21	2.939248669255165E-4	0.0	0.0	17.610606376797964	0.0
22	2.939248669255165E-4	0.0	0.0	18.601819003026446	0.0
23	2.939248669255165E-4	0.0	0.0	19.533952731002902	0.0
24	2.939248669255165E-4	0.0	0.0	20.20517915210514	0.0
25	2.939248669255165E-4	0.0	0.0	20.81742464991099	0.0
26	2.939248669255165E-4	0.0	0.0	21.38587534254494	0.0
27	3.9189982256735534E-4	0.0	0.0	22.002627688310316	0.0
28	3.9189982256735534E-4	0.0	0.0	22.591555146673407	0.0
29	3.9189982256735534E-4	0.0	0.0	23.275126412186516	0.0
30	3.9189982256735534E-4	0.0	0.0	24.07607167455855	0.0
31	3.9189982256735534E-4	0.0	0.0	24.687925272541833	0.0
32	3.9189982256735534E-4	0.0	0.0	25.28880567549323	0.0
33	3.9189982256735534E-4	0.0	0.0	25.87959465801352	0.0
34	3.9189982256735534E-4	0.0	0.0	26.58354471430013	0.0
35	3.9189982256735534E-4	0.0	0.0	27.281126398470022	0.0
36	3.9189982256735534E-4	0.0	0.0	27.88151692664321	0.0
37	3.9189982256735534E-4	0.0	0.0	28.510026267085607	0.0
38	3.9189982256735534E-4	0.0	0.0	29.322826499090304	0.0
39	3.9189982256735534E-4	0.0	0.0	30.90139898439161	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	3.8904975E-5	45.0	8
ACCGATA	20	7.032917E-4	45.0	10
GCGATCG	20	7.032917E-4	45.0	9
AACCCGA	55	6.184564E-11	40.909092	24
CATACGA	140	0.0	38.57143	18
TCTTGCG	205	0.0	38.414635	1
TACGGGA	130	0.0	38.07692	4
GTTTGCG	225	0.0	38.0	1
TTGGGCG	925	0.0	37.945946	5
TTATGGG	640	0.0	37.617188	3
TTTGGGC	2125	0.0	37.588234	4
GAATGCG	30	1.13983246E-4	37.499996	41
CCGATCA	30	1.13983246E-4	37.499996	10
CGAATAT	60	1.546141E-10	37.499996	14
TTACGCG	30	1.13983246E-4	37.499996	1
GACCGAT	465	0.0	37.258064	9
CGGGATA	260	0.0	37.211536	6
TGGGCGA	1205	0.0	37.157677	6
TTTGGGA	4580	0.0	36.94323	4
TTTCGCG	295	0.0	36.61017	1
>>END_MODULE