##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933867.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368111
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36468891176846	33.0	31.0	34.0	31.0	34.0
2	32.59949037111089	34.0	31.0	34.0	31.0	34.0
3	32.658947980364616	34.0	31.0	34.0	31.0	34.0
4	36.02559282390366	37.0	35.0	37.0	35.0	37.0
5	36.07640086821638	37.0	35.0	37.0	35.0	37.0
6	35.71970682756017	37.0	35.0	37.0	35.0	37.0
7	36.030675529935266	37.0	35.0	37.0	35.0	37.0
8	36.038366144994306	37.0	36.0	37.0	35.0	37.0
9	37.911130066746175	39.0	38.0	39.0	35.0	39.0
10	37.536430587513	39.0	37.0	39.0	35.0	39.0
11	37.438066235456155	39.0	37.0	39.0	35.0	39.0
12	37.21995539388934	39.0	37.0	39.0	34.0	39.0
13	37.07523817544165	39.0	37.0	39.0	33.0	39.0
14	38.25549358753202	40.0	37.0	41.0	33.0	41.0
15	38.350962073939655	40.0	37.0	41.0	34.0	41.0
16	38.45247493283276	40.0	37.0	41.0	34.0	41.0
17	38.45472153779702	40.0	37.0	41.0	34.0	41.0
18	38.42171790574039	40.0	37.0	41.0	35.0	41.0
19	38.439318575103705	40.0	37.0	41.0	34.0	41.0
20	38.3897357047195	40.0	37.0	41.0	34.0	41.0
21	38.269853386614365	40.0	37.0	41.0	34.0	41.0
22	38.27244499621038	40.0	37.0	41.0	34.0	41.0
23	38.216029947488664	40.0	37.0	41.0	34.0	41.0
24	38.20563362681365	40.0	37.0	41.0	34.0	41.0
25	38.10276791511256	40.0	36.0	41.0	34.0	41.0
26	38.01226532214468	40.0	36.0	41.0	34.0	41.0
27	38.0224388839236	40.0	36.0	41.0	34.0	41.0
28	37.975683964891026	40.0	36.0	41.0	34.0	41.0
29	37.973728033120445	40.0	36.0	41.0	34.0	41.0
30	37.91955687279109	40.0	36.0	41.0	34.0	41.0
31	37.84387046298535	40.0	36.0	41.0	34.0	41.0
32	37.723295419044796	40.0	36.0	41.0	33.0	41.0
33	37.64624800671537	40.0	36.0	41.0	33.0	41.0
34	37.652066903732845	40.0	36.0	41.0	33.0	41.0
35	37.60812092004857	40.0	36.0	41.0	33.0	41.0
36	37.4946551447797	40.0	35.0	41.0	33.0	41.0
37	37.46181722360918	40.0	35.0	41.0	33.0	41.0
38	37.42298654481936	40.0	35.0	41.0	33.0	41.0
39	37.402199336613144	40.0	35.0	41.0	33.0	41.0
40	37.30020021134929	39.0	35.0	41.0	33.0	41.0
41	37.23806949534244	39.0	35.0	41.0	33.0	41.0
42	37.191711195807784	39.0	35.0	41.0	33.0	41.0
43	37.09000817688143	39.0	35.0	41.0	32.0	41.0
44	36.970734370882695	39.0	35.0	41.0	32.0	41.0
45	37.02613885485628	39.0	35.0	41.0	32.0	41.0
46	36.96451070465159	39.0	35.0	41.0	32.0	41.0
47	36.91058131922165	39.0	35.0	41.0	32.0	41.0
48	36.88975336243687	39.0	35.0	41.0	32.0	41.0
49	36.87207662906026	39.0	35.0	41.0	32.0	41.0
50	36.816074499267884	39.0	35.0	41.0	32.0	41.0
51	35.89133169071285	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	12.0
11	8.0
12	5.0
13	5.0
14	4.0
15	10.0
16	14.0
17	33.0
18	50.0
19	82.0
20	157.0
21	242.0
22	372.0
23	602.0
24	970.0
25	1383.0
26	1883.0
27	2304.0
28	2691.0
29	3301.0
30	4195.0
31	5543.0
32	7434.0
33	10847.0
34	21880.0
35	33810.0
36	25445.0
37	38823.0
38	79117.0
39	126834.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.942837894004796	4.869726794363657	55.033943565935274	18.15349174569627
2	18.71419218659589	4.875159938170824	54.36322196294052	22.04742591229276
3	21.00806550198174	4.012105044402368	52.37550630108853	22.60432315252736
4	19.374319159166664	4.551616224454038	51.847133065841554	24.226931550537746
5	17.608819079027793	5.936252923710511	51.04329943957122	25.41162855769048
6	21.05587716748481	6.518957597029157	53.1347881481401	19.290377087345938
7	81.4990587078354	2.2933300010051316	10.504711893966766	5.702899397192695
8	82.58731741241093	2.838002667673609	7.999760941672484	6.574918978242976
9	76.75347924946551	5.093029004838215	11.56960808017147	6.583883665524801
10	40.217760403791246	23.03734471395856	20.10290374370774	16.64199113854245
11	29.291708207578694	26.65364523200882	25.380659637989627	18.673986922422856
12	27.14588806093814	22.720864087191092	30.096085148229747	20.03716270364102
13	22.929768466576657	26.576766247137414	30.494062932104722	19.99940235418121
14	18.497409748689932	29.15397801206701	30.31721410118144	22.03139813806162
15	15.42714018325994	26.17199703350348	38.25802543254616	20.142837350690417
16	16.749295728733994	25.70556163765821	35.13315277185414	22.411989861753657
17	17.775888251098173	24.848211544887274	30.238976830358233	27.136923373656312
18	18.39173510164054	24.436379244304028	33.690109776670624	23.481775877384813
19	21.862699022849085	25.790861995430724	31.04009388472499	21.306345096995198
20	24.66375631263396	25.83595708903021	29.62204335105444	19.87824324728139
21	21.74235488752034	27.238523162850335	30.66466364764976	20.354458301979566
22	20.53266541885464	25.473294739901824	28.112987658613843	25.881052182629695
23	19.863030444621323	26.809304802084156	28.390621307160067	24.937043446134453
24	20.6861517314071	24.007160883537846	30.62418672628636	24.682500658768685
25	18.909785363653896	26.699283639989023	29.23466019760344	25.15627079875364
26	17.690859550516013	30.260166091206187	28.735625938915167	23.313348419362637
27	20.028741330739912	29.247156428359922	28.692432445648187	22.031669795251975
28	17.03073257794524	25.872087495347873	33.224217695206065	23.872962231500825
29	18.27302090945394	25.39424249750755	31.844199168185682	24.48853742485283
30	20.607914460583903	27.370276900174133	28.432456514475252	23.589352124766712
31	20.50604301419952	28.656573696520887	25.287481221696716	25.549902067582874
32	20.65735606922912	29.063244510487323	25.387722724938943	24.89167669534461
33	21.45711483764408	29.135505323122647	25.15110931213683	24.256270527096447
34	18.156751631980573	27.226841903664926	27.776676056950212	26.83973040740429
35	17.664508803051255	26.65364523200882	27.297201115967738	28.384644848972187
36	22.05557562800351	27.997261695521185	26.568073217045946	23.379089459429355
37	19.52373061386375	27.342024552376863	28.882049164518307	24.252195669241072
38	17.389320069218254	28.162700924449418	27.893760305994658	26.554218700337668
39	18.756570708291793	27.69898210050773	29.506045730771426	24.038401460429053
40	19.998587382610136	26.340967805906367	28.77800446061107	24.88244035087243
41	17.399099728071153	25.823732515464087	26.020140664093166	30.757027092371597
42	20.199070389094594	25.97015574106723	26.283376481550402	27.54739738828777
43	21.620109151859086	25.901154814716215	27.371635186125925	25.107100847298774
44	19.126024487179137	26.128803540236508	28.321892037999408	26.423279934584947
45	19.093697281526495	25.81639777132441	27.769069655620182	27.320835291528915
46	20.635080179619735	25.73082575636153	28.500914126445554	25.133179937573182
47	17.642776227822587	25.26357538894518	30.031702394114816	27.061945989117415
48	17.781593052095698	24.093276212881438	30.447881209743798	27.67724952527906
49	19.33248395185148	22.765687523600217	31.676858338924944	26.22497018562336
50	19.82065192292542	23.29161584413397	28.891013851800135	27.99671838114047
51	17.57540524461372	23.486394049620902	27.010331122949328	31.927869582816054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	219.0
2	375.0
3	803.0
4	1231.0
5	828.5
6	426.0
7	478.0
8	530.0
9	604.0
10	678.0
11	703.0
12	728.0
13	725.5
14	723.0
15	744.0
16	765.0
17	671.0
18	577.0
19	711.5
20	846.0
21	889.5
22	933.0
23	1090.5
24	1248.0
25	1595.0
26	2509.5
27	3077.0
28	3647.0
29	4217.0
30	4911.0
31	5605.0
32	6493.5
33	7382.0
34	8349.5
35	9317.0
36	10098.0
37	10879.0
38	12613.0
39	14347.0
40	16810.5
41	19274.0
42	22553.5
43	25833.0
44	28779.5
45	31726.0
46	35885.0
47	40044.0
48	45319.5
49	50595.0
50	47393.0
51	44191.0
52	35740.0
53	27289.0
54	22263.5
55	17238.0
56	14312.5
57	11387.0
58	10805.5
59	10224.0
60	9291.5
61	8359.0
62	7152.5
63	5946.0
64	4840.0
65	3734.0
66	3099.5
67	2465.0
68	1980.5
69	1496.0
70	1203.0
71	910.0
72	760.5
73	611.0
74	504.0
75	331.5
76	266.0
77	179.5
78	93.0
79	93.5
80	94.0
81	58.5
82	23.0
83	17.0
84	11.0
85	9.0
86	7.0
87	6.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.778635069889965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.54650841560041	22.796396197399975
2	12.898162774158154	8.455683412923396
3	5.12737710231677	5.042052687076539
4	2.7154460791025277	3.5603446431546124
5	1.5250441509664918	2.499443284500041
6	0.9826618872672224	1.9326189239892981
7	0.6652717890376615	1.5264690836610832
8	0.5556232815237926	1.4570058225122509
9	0.404464513408607	1.193201521536721
>10	5.282113502835303	40.72857259673307
>50	0.245834204245226	4.996300227998408
>100	0.04567865281583591	2.7043669408236357
>500	0.0024915628808637774	0.6095306604091122
>1k	0.003322083841151703	2.498013997281828
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	2686	0.7296712133025093	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	2530	0.6872926916066078	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	2162	0.5873228455547376	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	1798	0.4884396282643007	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	837	0.22737706832993307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.20863272219520743	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	634	0.17223065868718945	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	496	0.13474196641773814	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	487	0.13229705170451303	No Hit
TGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	438	0.11898584937695424	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.71657190358343E-4	0.0	0.0	0.712828467500292	0.0
2	2.71657190358343E-4	0.0	0.0	2.665228694605703	0.0
3	2.71657190358343E-4	0.0	0.0	3.7543023707523004	0.0
4	2.71657190358343E-4	0.0	0.0	4.77899329278397	0.0
5	2.71657190358343E-4	0.0	0.0	6.37905414399461	0.0
6	2.71657190358343E-4	0.0	0.0	7.503171597697434	0.0
7	2.71657190358343E-4	0.0	0.0	8.568611098282854	0.0
8	2.71657190358343E-4	0.0	0.0	10.404470390724537	0.0
9	2.71657190358343E-4	0.0	0.0	11.395204163961413	0.0
10	2.71657190358343E-4	0.0	0.0	13.00069815897922	0.0
11	2.71657190358343E-4	0.0	0.0	15.57003186538843	0.0
12	2.71657190358343E-4	0.0	0.0	17.90302381618588	0.0
13	2.71657190358343E-4	0.0	0.0	18.629978457584805	0.0
14	2.71657190358343E-4	0.0	0.0	18.87365495733624	0.0
15	2.71657190358343E-4	0.0	0.0	19.25696325293186	0.0
16	2.71657190358343E-4	0.0	0.0	20.228952680034013	0.0
17	2.71657190358343E-4	0.0	0.0	21.597018290678626	0.0
18	2.71657190358343E-4	0.0	0.0	23.29188750132433	0.0
19	2.71657190358343E-4	0.0	0.0	24.24757749700498	0.0
20	2.71657190358343E-4	0.0	0.0	25.312202026019325	0.0
21	2.71657190358343E-4	0.0	0.0	26.694665467752934	0.0
22	2.71657190358343E-4	0.0	0.0	28.040998503168883	0.0
23	2.71657190358343E-4	0.0	0.0	29.29007826443654	0.0
24	2.71657190358343E-4	0.0	0.0	30.27755215138912	0.0
25	5.43314380716686E-4	0.0	0.0	31.1397920735865	0.0
26	5.43314380716686E-4	0.0	0.0	31.870549915650443	0.0
27	5.43314380716686E-4	0.0	0.0	32.58337838315074	0.0
28	5.43314380716686E-4	0.0	0.0	33.312506282072526	0.0
29	0.001086628761433372	0.0	0.0	34.10900516420319	0.0
30	0.001086628761433372	0.0	0.0	34.93049650784682	0.0
31	0.001086628761433372	0.0	0.0	35.79409471599599	0.0
32	0.001086628761433372	0.0	0.0	36.55201827709577	0.0
33	0.001086628761433372	0.0	0.0	37.28440606230186	0.0
34	0.001086628761433372	0.0	0.0	37.979033498048146	0.0
35	0.001086628761433372	0.0	0.0	38.78884358250637	0.0
36	0.001086628761433372	0.0	0.0	39.527751140281055	0.0
37	0.001086628761433372	0.0	0.0	40.24057960778135	0.0
38	0.001086628761433372	0.0	0.0	40.921352526819355	0.0
39	0.001086628761433372	0.0	0.0	41.66542157121086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	7.027031E-4	45.000004	26
AATCCGA	20	7.027031E-4	45.000004	23
TCGCAAG	20	7.027031E-4	45.000004	31
GATCGGA	20	7.027031E-4	45.000004	9
TCCGAAA	20	7.027031E-4	45.000004	25
TATATCG	20	7.027031E-4	45.000004	19
CACGAGT	40	6.7939254E-9	45.000004	36
TACGGGA	20	7.027031E-4	45.000004	4
CCAGATG	20	7.027031E-4	45.000004	12
GACCCGT	20	7.027031E-4	45.000004	40
AGAACGT	20	7.027031E-4	45.000004	24
ATATCGG	20	7.027031E-4	45.000004	20
TACGCGG	20	7.027031E-4	45.000004	2
TGTTACG	20	7.027031E-4	45.000004	1
GAACGTA	20	7.027031E-4	45.000004	25
CGGTGAC	20	7.027031E-4	45.000004	23
GCCTATA	20	7.027031E-4	45.000004	11
TACTCAC	20	7.027031E-4	45.000004	30
CTTTCCG	20	7.027031E-4	45.000004	1
CCGAAAC	20	7.027031E-4	45.000004	26
>>END_MODULE