##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933866.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1435216
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.396643432068764	33.0	31.0	34.0	31.0	34.0
2	32.64811916812521	34.0	31.0	34.0	31.0	34.0
3	32.63608683292271	34.0	31.0	34.0	31.0	34.0
4	36.07191391400319	37.0	35.0	37.0	35.0	37.0
5	36.1141382203097	37.0	35.0	37.0	35.0	37.0
6	35.75117403930837	37.0	35.0	37.0	35.0	37.0
7	36.063285247656104	37.0	35.0	37.0	35.0	37.0
8	36.064011967536594	37.0	36.0	37.0	35.0	37.0
9	37.94234317343174	39.0	38.0	39.0	35.0	39.0
10	37.651232288380285	39.0	37.0	39.0	35.0	39.0
11	37.555351947024	39.0	37.0	39.0	35.0	39.0
12	37.32519913378892	39.0	37.0	39.0	35.0	39.0
13	37.231400709022196	39.0	37.0	39.0	34.0	39.0
14	38.22359561208905	40.0	38.0	41.0	33.0	41.0
15	38.35420661419605	40.0	38.0	41.0	33.0	41.0
16	38.513708041158964	40.0	38.0	41.0	34.0	41.0
17	38.51725384889801	40.0	38.0	41.0	34.0	41.0
18	38.392084536404276	40.0	38.0	41.0	34.0	41.0
19	38.318345809968676	40.0	37.0	41.0	34.0	41.0
20	38.15093198515067	40.0	36.0	41.0	34.0	41.0
21	38.07558235136732	40.0	36.0	41.0	34.0	41.0
22	38.12972820815821	40.0	36.0	41.0	34.0	41.0
23	38.080692383585465	40.0	36.0	41.0	34.0	41.0
24	38.04382058171035	40.0	36.0	41.0	34.0	41.0
25	37.950525217110176	40.0	35.0	41.0	34.0	41.0
26	37.88316741173454	40.0	35.0	41.0	34.0	41.0
27	37.85670240577028	40.0	35.0	41.0	34.0	41.0
28	37.75919443484465	40.0	35.0	41.0	34.0	41.0
29	37.70865082329071	40.0	35.0	41.0	34.0	41.0
30	37.604782834082116	40.0	35.0	41.0	33.0	41.0
31	37.41624884337967	40.0	35.0	41.0	33.0	41.0
32	37.20364321467988	40.0	35.0	41.0	32.0	41.0
33	36.97404362827616	39.0	35.0	41.0	32.0	41.0
34	36.74899945374076	39.0	35.0	41.0	31.0	41.0
35	36.59863811440229	39.0	35.0	41.0	31.0	41.0
36	36.442638599346715	39.0	35.0	41.0	30.0	41.0
37	36.31882309004359	39.0	35.0	41.0	30.0	41.0
38	36.249280944471074	39.0	35.0	41.0	30.0	41.0
39	36.19975251112028	39.0	35.0	41.0	30.0	41.0
40	36.0731304556248	39.0	35.0	41.0	29.0	41.0
41	35.902381941115486	39.0	35.0	41.0	29.0	41.0
42	35.85045874627931	39.0	35.0	41.0	29.0	41.0
43	35.779932776669156	39.0	35.0	41.0	28.0	41.0
44	35.69894845096487	39.0	35.0	41.0	28.0	41.0
45	35.73062939655076	39.0	35.0	41.0	28.0	41.0
46	35.664251234657364	38.0	35.0	41.0	28.0	41.0
47	35.55288890313374	38.0	35.0	40.0	28.0	41.0
48	35.451557117534925	38.0	35.0	40.0	27.0	41.0
49	35.43503277555434	38.0	35.0	40.0	27.0	41.0
50	35.347512151481034	38.0	35.0	40.0	27.0	41.0
51	34.38899440920391	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	26.0
10	40.0
11	21.0
12	13.0
13	25.0
14	19.0
15	51.0
16	99.0
17	190.0
18	323.0
19	594.0
20	1113.0
21	1785.0
22	2799.0
23	4563.0
24	7356.0
25	12390.0
26	17770.0
27	20186.0
28	19665.0
29	19502.0
30	21462.0
31	25750.0
32	33607.0
33	49535.0
34	94139.0
35	96165.0
36	100877.0
37	156180.0
38	289900.0
39	458805.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.11324567173164	4.611988718074492	52.16629413272985	20.10847147746402
2	24.237884750448714	5.489696324455692	49.55010256295916	20.722316362136432
3	18.91150879031449	5.692383585467275	53.546574174200956	21.84953345001728
4	17.567460228982952	4.487199139362994	54.283188035807854	23.6621525958462
5	18.435622233865843	5.255515546092017	52.34445546872387	23.96440675131827
6	20.908281401545135	6.740866879967894	54.34039196887437	18.0104597496126
7	78.8716820325303	2.012031638443273	14.241340676246642	4.8749456527797905
8	80.29321022062184	3.267034369739468	11.898278725989677	4.5414766836490115
9	75.06744629379828	4.483645667272382	13.870455736279416	6.578452302649914
10	39.24301289840693	26.71716313084581	20.284333507987647	13.755490462759612
11	29.35586002385704	22.00128761106342	30.185630594976644	18.457221770102898
12	26.126520328647395	19.977132361958063	33.29429159095216	20.60205571844238
13	25.142278235471178	19.560540016276295	36.06725398824985	19.229927760002678
14	19.97971037112184	22.12621654162161	37.29459537797795	20.599477709278606
15	17.61233152361735	22.200003344444323	39.288371924504744	20.899293207433583
16	22.9943785465045	23.32345793246452	33.16065316997581	20.521510351055174
17	23.94357365023801	23.47151926957336	31.62680739345158	20.958099686737047
18	23.779277822989712	22.751697305492694	32.75994693481678	20.70907793670082
19	23.702843335079876	24.57344399727985	31.040623850347266	20.683088817293008
20	22.596738051972665	27.934331835765487	30.697539603794834	18.771390508467018
21	22.812594062496515	25.690279372582246	33.198835575969056	18.298290988952186
22	21.957809834895933	21.971326964024925	34.33894270966879	21.731920491410353
23	20.64448835575969	24.10975072741664	33.29268904471522	21.95307187210845
24	21.471402214022138	25.16269328101136	31.183180789511823	22.182723715454678
25	23.19142205772511	25.418125215995364	29.041691285492917	22.348761440786614
26	21.267391110466995	24.56738219194881	29.822270654730715	24.34295604285348
27	21.962060066219998	23.492073666960234	32.5640182383697	21.981848028450074
28	20.056702266418437	23.72911115818107	31.6242293842878	24.589957191112696
29	22.30514431277243	23.89981717037714	30.312301423618464	23.482737093231957
30	25.726580528645165	22.200212372214356	30.684440530205908	21.38876656893457
31	24.60723682010234	22.76751374009208	30.955758575712643	21.66949086409293
32	26.33443328390988	23.529280610026643	28.73741652824383	21.398869577819646
33	25.93191547474387	23.17240053065183	28.862972542112132	22.03271145249217
34	23.146063031627296	23.210164881105005	31.57880068226664	22.06497140500106
35	23.456538946054113	26.22699301011137	28.193317242840106	22.123150800994416
36	25.734105528366463	25.942924270632435	27.764531610572902	20.5584385904282
37	23.047610951940335	27.4262550027313	28.883387587652308	20.642746457676058
38	22.670246151101995	26.066041627183644	29.098964894482783	22.16474732723158
39	22.312878340263765	24.73153866735042	28.680212595177313	24.275370397208505
40	24.655800938674037	24.22506438055317	30.12982018037703	20.989314500395757
41	22.773296841729746	25.404538410943022	28.02797627674162	23.794188470585613
42	21.72822766747305	25.81959788631119	30.20451277020323	22.24766167601253
43	22.022608443607094	24.149396327800137	30.904825475747206	22.923169752845567
44	23.028798452637094	23.02886812856044	29.61024681999086	24.332086598811607
45	22.32151815475859	21.992578120645252	28.74403574096164	26.94186798363452
46	23.068583404867283	22.725638510161534	29.408813725599494	24.79696435937169
47	20.988617741162304	23.747784305637616	32.0828363117468	23.180761641453273
48	21.065888340152284	24.953177779511932	30.229665778530894	23.751268101804886
49	22.83210332103321	22.41530194758141	31.17739768787416	23.57519704351122
50	21.42855152116476	23.06593571978016	30.087526894906414	25.417985864148672
51	21.00352838875821	23.21336997357889	28.788001248592547	26.995100389070352
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	787.0
2	1345.0
3	12194.0
4	23043.0
5	15065.0
6	7087.0
7	7123.0
8	7159.0
9	7428.0
10	7697.0
11	7635.5
12	7574.0
13	7507.0
14	7440.0
15	7106.0
16	6772.0
17	6612.0
18	6452.0
19	6268.0
20	6084.0
21	6218.5
22	6353.0
23	6524.0
24	6695.0
25	7578.0
26	9777.0
27	11093.0
28	13241.5
29	15390.0
30	17630.0
31	19870.0
32	22643.5
33	25417.0
34	28746.5
35	32076.0
36	34772.5
37	37469.0
38	41255.0
39	45041.0
40	49820.0
41	54599.0
42	61374.0
43	68149.0
44	76387.5
45	84626.0
46	110118.5
47	135611.0
48	143628.0
49	151645.0
50	150216.0
51	148787.0
52	130868.0
53	112949.0
54	100266.0
55	87583.0
56	81404.0
57	75225.0
58	67964.5
59	60704.0
60	53880.5
61	47057.0
62	41827.5
63	36598.0
64	31676.5
65	26755.0
66	22999.0
67	19243.0
68	16320.5
69	13398.0
70	11122.0
71	8846.0
72	7595.5
73	6345.0
74	5286.0
75	3080.0
76	1933.0
77	1496.0
78	1059.0
79	899.5
80	740.0
81	495.0
82	250.0
83	154.5
84	59.0
85	59.5
86	60.0
87	38.5
88	17.0
89	10.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1435216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.764169887038236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89089287648659	24.94864660186637
2	9.824759691779231	6.634497106651188
3	3.564730955542324	3.610805447535455
4	1.8887275624303752	2.550852731529232
5	1.2671990770837955	2.139296245967767
6	0.9567819396615076	1.9382968773348668
7	0.8132815728793257	1.9221844034886655
8	0.6620090037260614	1.7881747574844535
9	0.5987077315694752	1.8193382605255544
>10	6.356019184786943	41.88638188186817
>50	0.11704510902302495	2.640720715666221
>100	0.05296413129793836	3.131689414207115
>500	0.0029192815838772106	0.6783529963571313
>1k	0.0037533620364135563	3.252926153678105
>5k	0.0	0.0
>10k+	2.0852011313408645E-4	1.0578364058397403
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15025	1.0468807482636762	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	4755	0.33130901550707353	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	4269	0.2974465167612401	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	3986	0.27772823045451	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTG	3761	0.2620511477018093	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT	3538	0.24651341679579938	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	3489	0.2430992965518779	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	3033	0.2113270755064046	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	2911	0.2028266128582736	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	2863	0.19948216853769746	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	2703	0.18833402080244366	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	2212	0.15412314243988362	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	1806	0.12583471756167713	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.967592334533617E-5	0.0	0.0	0.29222082251033993	0.0
2	6.967592334533617E-5	0.0	0.0	0.9957386205281993	0.0
3	6.967592334533617E-5	0.0	0.0	1.6060997090333442	0.0
4	6.967592334533617E-5	0.0	0.0	2.2991661185494032	0.0
5	6.967592334533617E-5	0.0	0.0	3.771488054759702	0.0
6	6.967592334533617E-5	0.0	0.0	5.117766245638287	0.0
7	6.967592334533617E-5	0.0	0.0	6.288182405993244	0.0
8	6.967592334533617E-5	0.0	0.0	7.844533505758018	0.0
9	6.967592334533617E-5	0.0	0.0	8.907230688621086	0.0
10	6.967592334533617E-5	0.0	0.0	10.374466282427175	0.0
11	6.967592334533617E-5	0.0	0.0	12.097342838987302	0.0
12	6.967592334533617E-5	0.0	0.0	13.730198102585256	0.0
13	1.3935184669067234E-4	0.0	0.0	14.297917526003054	0.0
14	1.3935184669067234E-4	0.0	0.0	14.620168671475234	0.0
15	1.3935184669067234E-4	0.0	0.0	14.930226530361981	0.0
16	1.3935184669067234E-4	0.0	0.0	15.404162160956957	0.0
17	1.3935184669067234E-4	0.0	0.0	16.026716536047537	0.0
18	1.3935184669067234E-4	0.0	0.0	16.67964961371668	0.0
19	1.3935184669067234E-4	0.0	0.0	17.246114870514266	0.0
20	1.3935184669067234E-4	0.0	0.0	17.82477341389728	0.0
21	1.3935184669067234E-4	0.0	0.0	18.59566782979008	0.0
22	1.3935184669067234E-4	0.0	0.0	19.47086710293085	0.0
23	1.3935184669067234E-4	0.0	0.0	20.226920547151092	0.0
24	1.3935184669067234E-4	0.0	0.0	20.819165895586448	0.0
25	1.3935184669067234E-4	0.0	0.0	21.36026911628633	0.0
26	1.3935184669067234E-4	0.0	0.0	21.843889700226306	0.0
27	1.3935184669067234E-4	0.0	0.0	22.40004292036878	0.0
28	1.3935184669067234E-4	0.0	0.0	22.90874683671308	0.0
29	1.3935184669067234E-4	0.0	0.0	23.488520194869622	0.0
30	1.3935184669067234E-4	0.0	0.0	24.158384521911685	0.0
31	1.3935184669067234E-4	0.0	0.0	24.712795843970525	0.0
32	1.3935184669067234E-4	0.0	0.0	25.25564096275404	0.0
33	2.787036933813447E-4	0.0	0.0	25.7753536749869	0.0
34	2.787036933813447E-4	0.0	0.0	26.33694161715031	0.0
35	2.787036933813447E-4	0.0	0.0	26.95287677952308	0.0
36	2.787036933813447E-4	0.0	0.0	27.47579458422983	0.0
37	2.787036933813447E-4	0.0	0.0	28.055985997926445	0.0
38	2.787036933813447E-4	0.0	0.0	28.74898273151916	0.0
39	2.787036933813447E-4	0.0	0.0	30.042585924348668	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8220	0.0	44.041973	1
GTACGGG	180	0.0	41.250004	3
TACGGGA	160	0.0	39.375004	4
TTTCGCG	450	0.0	39.0	1
TGGGACG	365	0.0	38.219177	6
TTGGGAC	3000	0.0	38.025	5
TGGGCGA	1435	0.0	37.787457	6
CGTTCTG	490	0.0	37.65306	1
GTTTTTT	9780	0.0	37.638035	2
CGTTGAT	30	1.14006325E-4	37.500004	25
GCGATGT	300	0.0	37.500004	9
TAAGCGC	30	1.14006325E-4	37.500004	32
TTTGGGA	7490	0.0	37.429905	4
TTACGGG	295	0.0	37.372883	3
TTTGGGC	2900	0.0	37.318966	4
TGGGACT	1965	0.0	37.213737	6
TCGTTTG	375	0.0	37.2	1
TTGTTCG	375	0.0	37.2	1
TACGGGG	310	0.0	37.016132	4
TTGGGAT	4525	0.0	36.745857	5
>>END_MODULE