##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933865.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1885202
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.365243088008604	33.0	31.0	34.0	31.0	34.0
2	32.58793169113973	34.0	31.0	34.0	31.0	34.0
3	32.63491392434339	34.0	31.0	34.0	31.0	34.0
4	36.034636076134014	37.0	35.0	37.0	35.0	37.0
5	36.06963603900272	37.0	35.0	37.0	35.0	37.0
6	35.71277295483455	37.0	35.0	37.0	35.0	37.0
7	36.02541902671438	37.0	35.0	37.0	35.0	37.0
8	36.03762832842316	37.0	35.0	37.0	35.0	37.0
9	37.87458744474067	39.0	38.0	39.0	35.0	39.0
10	37.55438621431549	39.0	37.0	39.0	35.0	39.0
11	37.483680263441265	39.0	37.0	39.0	35.0	39.0
12	37.22383118626015	39.0	37.0	39.0	34.0	39.0
13	37.11554623854632	39.0	37.0	39.0	34.0	39.0
14	38.02862823188178	40.0	38.0	41.0	33.0	41.0
15	38.170601877146325	40.0	37.0	41.0	33.0	41.0
16	38.33766620234861	40.0	38.0	41.0	33.0	41.0
17	38.35850375715706	40.0	37.0	41.0	34.0	41.0
18	38.297276896587206	40.0	37.0	41.0	34.0	41.0
19	38.30071631581125	40.0	37.0	41.0	34.0	41.0
20	38.184351066888325	40.0	37.0	41.0	34.0	41.0
21	38.10111065021149	40.0	36.0	41.0	34.0	41.0
22	38.151397038619734	40.0	36.0	41.0	34.0	41.0
23	38.119778676237345	40.0	36.0	41.0	34.0	41.0
24	38.07789987492057	40.0	36.0	41.0	34.0	41.0
25	37.97864632012909	40.0	36.0	41.0	34.0	41.0
26	37.92249955177217	40.0	36.0	41.0	34.0	41.0
27	37.92307614780803	40.0	36.0	41.0	34.0	41.0
28	37.84486490041916	40.0	36.0	41.0	34.0	41.0
29	37.81538105730845	40.0	36.0	41.0	34.0	41.0
30	37.7347875718358	40.0	36.0	41.0	33.0	41.0
31	37.617487675060815	40.0	35.0	41.0	33.0	41.0
32	37.53252012251207	40.0	35.0	41.0	33.0	41.0
33	37.443554589906014	40.0	35.0	41.0	33.0	41.0
34	37.325682340672245	40.0	35.0	41.0	32.0	41.0
35	37.237759667133815	40.0	35.0	41.0	32.0	41.0
36	37.14433095233296	40.0	35.0	41.0	32.0	41.0
37	37.06945356518824	39.0	35.0	41.0	32.0	41.0
38	37.030626426239735	39.0	35.0	41.0	32.0	41.0
39	36.98767399992149	39.0	35.0	41.0	32.0	41.0
40	36.87995716108937	39.0	35.0	41.0	31.0	41.0
41	36.706853164806745	39.0	35.0	41.0	31.0	41.0
42	36.67169194600897	39.0	35.0	41.0	31.0	41.0
43	36.573644097555594	39.0	35.0	41.0	31.0	41.0
44	36.49892637499854	39.0	35.0	41.0	31.0	41.0
45	36.51880435093958	39.0	35.0	41.0	31.0	41.0
46	36.47276631363642	39.0	35.0	41.0	31.0	41.0
47	36.3864227812192	39.0	35.0	41.0	31.0	41.0
48	36.30112157742247	38.0	35.0	40.0	31.0	41.0
49	36.29298929239413	38.0	35.0	40.0	31.0	41.0
50	36.19451443399699	38.0	35.0	40.0	31.0	41.0
51	35.22607656898306	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	25.0
10	38.0
11	41.0
12	25.0
13	25.0
14	42.0
15	61.0
16	110.0
17	213.0
18	354.0
19	604.0
20	1101.0
21	1749.0
22	2768.0
23	4066.0
24	6128.0
25	9535.0
26	13584.0
27	16185.0
28	18137.0
29	20943.0
30	25425.0
31	32664.0
32	44343.0
33	67646.0
34	131638.0
35	131884.0
36	133978.0
37	210467.0
38	393791.0
39	617322.0
40	299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.617308914376284	4.515325148180407	53.96689585519218	16.900470082251132
2	21.552968859570488	4.580676235225721	51.75206688726195	22.114288017941846
3	21.691786874828267	4.592929564046718	50.94318805093565	22.77209551018936
4	19.57334015134718	5.095952582269699	50.028166742874234	25.30254052350889
5	18.8774465547989	6.312692220780584	49.90229163771309	24.90756958670742
6	22.446400969233004	6.420426033921034	52.919262763353736	18.21391023349222
7	81.53327866191528	2.7363115464549685	10.65434897692661	5.076060814703146
8	82.67649832750018	3.1517046979580963	9.194558461109208	4.977238513432513
9	76.32513651057022	5.49909240495183	12.00417780163611	6.1715932828418385
10	40.72322223294904	23.762705535003676	19.87808203046676	15.63599020158052
11	31.552374758779166	22.923697301403244	25.867572811826	19.65635512799159
12	27.980343750961435	20.18775706794285	30.631200263950493	21.200698917145218
13	26.65512767332095	20.221281326881684	32.68259846955392	20.44099253024344
14	21.289760991129864	21.720377975410592	35.44484888091568	21.545012152543865
15	19.327583993651608	22.18828539328942	36.84814677684407	21.635983836214898
16	24.482469252631812	22.323231144460912	31.378069830182653	21.816229772724622
17	24.621128133749064	22.651418786952274	29.575133062663845	23.15232001663482
18	24.687168802070016	21.9903755671806	31.517683516143098	21.804772114606287
19	24.947300077127014	24.45260507892523	29.215012502638977	21.385082341308784
20	23.721224569038224	27.923745041645404	28.828528719999234	19.526501669317135
21	24.03312748448177	25.767000035009513	31.32651036865015	18.87336211185857
22	22.254697374604948	23.10150318109147	32.07847222737934	22.56532721692423
23	21.460246700353597	23.773844924840944	31.46050131497845	23.30540705982701
24	22.015306582530677	25.34773461942009	29.32858123426561	23.308377563783615
25	23.82736704077335	24.839672353413587	26.918812944183166	24.414147661629894
26	21.33352287977628	24.751936397266714	28.00405473790077	25.91048598505624
27	21.153011719699002	22.976795059627563	31.37451583437743	24.495677386296006
28	19.217304034262643	23.68255497288885	30.62451663004813	26.475624362800374
29	21.247696533315793	24.178682178355423	29.86284758874646	24.710773699582326
30	24.106488323267214	22.380625524479605	29.72148342724016	23.791402725013022
31	22.83930316220755	23.473611846369778	30.545798275198095	23.141286716224574
32	25.486976992385962	23.017109041895775	28.372980720368428	23.122933245349834
33	24.514932617300428	21.761540673094977	29.08632602766176	24.637200681942836
34	21.16733379234692	21.960988795895613	32.73214223197302	24.139535179784446
35	22.279257076960455	23.649402026944593	29.820411817937813	24.250929078157142
36	24.066970011701663	22.93467755709998	28.840835093533745	24.15751733766461
37	20.785040542074537	24.794372168075355	31.354040574962262	23.06654671488785
38	22.175660751473846	23.250877094337902	31.3105439098834	23.262918244304853
39	20.894843099041907	21.743346336360773	31.315848381234478	26.045962183362843
40	23.815219801379374	20.904072879192785	31.580488456939893	23.700218862487947
41	22.50177965013829	22.053392686831437	29.764131376902846	25.68069628612743
42	21.92396358586507	21.431125152636163	32.19400361340588	24.450907648092883
43	20.976054555426952	21.316177258458243	32.752723580815214	24.95504460529959
44	22.06278160112285	20.857181352449235	30.64196834079319	26.438068705634727
45	21.499711967205638	20.496901658283832	28.983154059883237	29.020232314627293
46	22.032705248562223	20.82079267898082	30.81484106212491	26.33166101033205
47	19.53345052678705	22.11916813158484	33.622020345830315	24.7253609957978
48	19.60071122351875	23.234114964868485	32.28216392726084	24.883009884351914
49	22.46634578151307	20.363653337944687	32.62271098800022	24.547289892542018
50	20.856863084168168	20.642880709865572	31.18222874790075	27.3180274580655
51	20.016157419735393	20.783820513663787	29.50898630491587	29.691035761684955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	985.5
2	1706.0
3	7773.0
4	13840.0
5	9039.0
6	4238.0
7	4143.5
8	4049.0
9	4258.5
10	4468.0
11	4473.0
12	4478.0
13	4372.0
14	4266.0
15	4149.5
16	4033.0
17	4050.0
18	4067.0
19	4083.0
20	4099.0
21	4672.0
22	5245.0
23	5926.5
24	6608.0
25	7518.5
26	10950.0
27	13471.0
28	16282.5
29	19094.0
30	22361.0
31	25628.0
32	29203.0
33	32778.0
34	37441.5
35	42105.0
36	46221.5
37	50338.0
38	56284.0
39	62230.0
40	68863.0
41	75496.0
42	82837.0
43	90178.0
44	101768.0
45	113358.0
46	135092.0
47	156826.0
48	185654.0
49	214482.0
50	215057.5
51	215633.0
52	188468.5
53	161304.0
54	143164.0
55	125024.0
56	114439.0
57	103854.0
58	95024.5
59	86195.0
60	76656.0
61	67117.0
62	57570.5
63	48024.0
64	42561.5
65	37099.0
66	32118.5
67	27138.0
68	22401.5
69	17665.0
70	14666.0
71	11667.0
72	9790.0
73	7913.0
74	6570.0
75	3991.0
76	2755.0
77	2182.5
78	1610.0
79	1174.5
80	739.0
81	510.0
82	281.0
83	184.5
84	88.0
85	64.0
86	40.0
87	42.5
88	45.0
89	24.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1885202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.78491436222757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.9291061814047	20.82832439040258
2	11.949798311124603	7.118474386854759
3	4.6976035240978185	4.197531560188559
4	2.4051780627194024	2.865520904960233
5	1.3873995346234298	2.066178816247662
6	0.9783656933669298	1.7484323035125242
7	0.7473100694429741	1.5580996494272525
8	0.5588040329299597	1.3315144212868997
9	0.4678097883850693	1.2540307036374982
>10	6.54194820236929	44.22355980301514
>50	0.24922774579205473	4.709412245106448
>100	0.07987604575480439	4.3586746258978
>500	0.004327318850917156	0.8681736194304599
>1k	0.0025242693297016752	1.458484421408662
>5k	7.212198084861929E-4	1.413588148623566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8691	0.46101160512242195	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	6452	0.3422444915717255	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	6042	0.32049615903229467	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	5137	0.2724906933050145	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	4806	0.2549328931329375	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	2929	0.15536796587315313	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2546	0.1350518405985141	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2370	0.12571597102061213	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	2167	0.11494789417791833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1217885404322718E-4	0.0	0.0	0.3579987714844351	0.0
2	2.1217885404322718E-4	0.0	0.0	1.26957217316765	0.0
3	2.1217885404322718E-4	0.0	0.0	1.9432930794684071	0.0
4	2.1217885404322718E-4	0.0	0.0	2.6611471874101555	0.0
5	2.1217885404322718E-4	0.0	0.0	3.92838539318333	0.0
6	2.1217885404322718E-4	0.0	0.0	5.045294880866878	0.0
7	2.1217885404322718E-4	0.0	0.0	6.015429646266023	0.0
8	2.1217885404322718E-4	0.0	0.0	7.470605272007987	0.0
9	2.1217885404322718E-4	0.0	0.0	8.342395138558096	0.0
10	2.1217885404322718E-4	0.0	0.0	9.65700227349642	0.0
11	2.65223567554034E-4	0.0	0.0	11.799955654619504	0.0
12	2.65223567554034E-4	0.0	0.0	13.620078909315819	0.0
13	2.65223567554034E-4	0.0	0.0	14.275552434168858	0.0
14	2.65223567554034E-4	0.0	0.0	14.582522191255897	0.0
15	2.65223567554034E-4	0.0	0.0	14.941528812297038	0.0
16	2.65223567554034E-4	0.0	0.0	15.645060847590868	0.0
17	2.65223567554034E-4	0.0	0.0	16.541410416496483	0.0
18	2.65223567554034E-4	0.0	0.0	17.45123334263384	0.0
19	2.65223567554034E-4	0.0	0.0	18.148028699311798	0.0
20	2.65223567554034E-4	0.0	0.0	18.977011482058685	0.0
21	2.65223567554034E-4	0.0	0.0	20.084744234304864	0.0
22	2.65223567554034E-4	0.0	0.0	21.213588782528344	0.0
23	2.65223567554034E-4	0.0	0.0	22.26069142723167	0.0
24	2.65223567554034E-4	0.0	0.0	23.089833344119093	0.0
25	3.182682810648408E-4	0.0	0.0	23.853518084534176	0.0
26	3.182682810648408E-4	0.0	0.0	24.501724483636238	0.0
27	3.182682810648408E-4	0.0	0.0	25.20626436848677	0.0
28	3.182682810648408E-4	0.0	0.0	25.877120860257946	0.0
29	3.713129945756476E-4	0.0	0.0	26.623513024068508	0.0
30	3.713129945756476E-4	0.0	0.0	27.544369250616114	0.0
31	3.713129945756476E-4	0.0	0.0	28.253683159682623	0.0
32	3.713129945756476E-4	0.0	0.0	28.9358381754316	0.0
33	3.713129945756476E-4	0.0	0.0	29.626692524196347	0.0
34	3.713129945756476E-4	0.0	0.0	30.397220032654324	0.0
35	3.713129945756476E-4	0.0	0.0	31.17734863425776	0.0
36	3.713129945756476E-4	0.0	0.0	31.888306929443104	0.0
37	3.713129945756476E-4	0.0	0.0	32.601758326163456	0.0
38	3.713129945756476E-4	0.0	0.0	33.47821612750252	0.0
39	3.713129945756476E-4	0.0	0.0	35.09119977593913	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4200	0.0	42.857143	1
TTTCGCG	530	0.0	40.33019	1
GTTACCG	70	0.0	38.571426	38
ATAACGC	35	6.2504187E-6	38.571426	11
TTGGGAC	3090	0.0	37.936893	5
CACGACG	30	1.1401992E-4	37.499996	26
GCGATGT	500	0.0	37.350002	9
CGCGACC	365	0.0	36.986298	10
TTTGGGA	7800	0.0	36.980766	4
TTTGGGC	4580	0.0	36.894108	4
TTGTTCG	465	0.0	36.774193	1
TGGGCGA	2420	0.0	36.446278	6
GGGCGAT	3550	0.0	36.316902	7
TAGTTCG	25	0.0021074496	36.000004	1
CGTTAGA	25	0.0021074496	36.000004	2
CCGTTGA	25	0.0021074496	36.000004	20
TTCGGGT	420	0.0	35.892857	4
TCAAGCG	240	0.0	35.625	17
GTTGGGA	2875	0.0	35.452175	4
TTGGGAG	4705	0.0	35.435707	5
>>END_MODULE