FastQCFastQC Report
Sat 14 Jan 2017
SRR2933864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113391
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT91200.43153396602900274TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC81860.38733958836769916TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC70430.33325589065156425TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC67060.31730995352965924TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.22357434095252607No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC31860.15075298418513186No Hit
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG28240.13362411404231397No Hit
CGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG25820.12217332239987773No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG25800.12207868775820471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT27650.040.280291
TCCGATA700.038.5714314
GCCGATT3650.038.2191779
TACGGGA3100.037.7419364
TTTGGGC48950.037.507664
GGCACCG6850.037.4452558
TTCTTCG6100.037.25411
TGGGCGA22900.036.943236
CTTTGCG7500.036.8999981
TTGGGAC40550.036.8434035
GCGCGAC4350.036.724149
TTTGGGA98600.036.716534
TTTCGCG7850.036.4012761
TTTCTCG7400.036.1824341
CGGGTAC2550.036.176476
CGTATAG250.002107507336.031
CTAACGT250.002107507336.041
CTAGTCG250.002107507336.01
TTGGGAT56200.035.9919935
CGGGTAT3900.035.769236
Produced by FastQC (version 0.11.5)