##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933863.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	868572
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.407110751900824	33.0	31.0	34.0	31.0	34.0
2	32.64047885494812	34.0	31.0	34.0	31.0	34.0
3	32.69482783235011	34.0	31.0	34.0	31.0	34.0
4	36.06701804801444	37.0	35.0	37.0	35.0	37.0
5	36.10453479964816	37.0	35.0	37.0	35.0	37.0
6	35.70146401219473	37.0	35.0	37.0	35.0	37.0
7	36.02645376549094	37.0	35.0	37.0	35.0	37.0
8	36.035888792178426	37.0	35.0	37.0	35.0	37.0
9	37.86660288381389	39.0	38.0	39.0	35.0	39.0
10	37.597150265032724	39.0	37.0	39.0	35.0	39.0
11	37.533465274036004	39.0	37.0	39.0	35.0	39.0
12	37.276291430071424	39.0	37.0	39.0	35.0	39.0
13	37.16429380638565	39.0	37.0	39.0	34.0	39.0
14	38.22509129928204	40.0	38.0	41.0	33.0	41.0
15	38.32891919150053	40.0	38.0	41.0	33.0	41.0
16	38.45222963669103	40.0	38.0	41.0	34.0	41.0
17	38.468070580216725	40.0	38.0	41.0	34.0	41.0
18	38.432385570798964	40.0	37.0	41.0	34.0	41.0
19	38.451904965851995	40.0	37.0	41.0	34.0	41.0
20	38.379707151508455	40.0	37.0	41.0	34.0	41.0
21	38.27014455911542	40.0	37.0	41.0	34.0	41.0
22	38.3131127874258	40.0	37.0	41.0	34.0	41.0
23	38.272277945869774	40.0	37.0	41.0	34.0	41.0
24	38.237636027871034	40.0	37.0	41.0	34.0	41.0
25	38.12966455285227	40.0	36.0	41.0	34.0	41.0
26	38.0651437071423	40.0	36.0	41.0	34.0	41.0
27	38.04726378469488	40.0	36.0	41.0	34.0	41.0
28	37.95749690296256	40.0	36.0	41.0	34.0	41.0
29	37.927879323763605	40.0	36.0	41.0	34.0	41.0
30	37.868503704931776	40.0	36.0	41.0	34.0	41.0
31	37.75145526220049	40.0	36.0	41.0	33.0	41.0
32	37.67011024992747	40.0	36.0	41.0	33.0	41.0
33	37.62353380030671	40.0	36.0	41.0	33.0	41.0
34	37.5491553952925	40.0	35.0	41.0	33.0	41.0
35	37.49816595515398	40.0	35.0	41.0	33.0	41.0
36	37.37768083705208	40.0	35.0	41.0	33.0	41.0
37	37.2893530991098	39.0	35.0	41.0	33.0	41.0
38	37.24851134966359	39.0	35.0	41.0	33.0	41.0
39	37.23044606549601	39.0	35.0	41.0	33.0	41.0
40	37.11179038698001	39.0	35.0	41.0	32.0	41.0
41	36.984171720939656	39.0	35.0	41.0	32.0	41.0
42	36.95806334995832	39.0	35.0	41.0	32.0	41.0
43	36.85079532842413	39.0	35.0	41.0	32.0	41.0
44	36.73000050657861	39.0	35.0	41.0	31.0	41.0
45	36.71816498804935	39.0	35.0	41.0	31.0	41.0
46	36.63911109269007	39.0	35.0	41.0	31.0	41.0
47	36.542353426083274	38.0	35.0	40.0	31.0	41.0
48	36.50030049322336	38.0	35.0	40.0	31.0	41.0
49	36.49422039853921	38.0	35.0	40.0	31.0	41.0
50	36.399712401505	38.0	35.0	40.0	31.0	41.0
51	35.3508517428607	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	20.0
11	17.0
12	19.0
13	14.0
14	11.0
15	13.0
16	34.0
17	64.0
18	124.0
19	258.0
20	421.0
21	712.0
22	1092.0
23	1606.0
24	2279.0
25	3319.0
26	4377.0
27	5819.0
28	6603.0
29	8091.0
30	10544.0
31	13960.0
32	19073.0
33	28960.0
34	57058.0
35	67729.0
36	64203.0
37	101545.0
38	187872.0
39	282522.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.301580064749956	5.048976941462539	55.14177293304412	16.507670060743383
2	20.79079224289984	4.991641452867465	52.60243249839968	21.615133805833022
3	22.694721911367164	5.1454571411466175	49.83236853133649	22.327452416149725
4	20.153539372671464	4.944207273547847	49.864950746742934	25.03730260703776
5	19.496599015395386	6.250028782875801	48.5793923819787	25.67397981975012
6	22.223834063267063	7.32178794619214	51.44259773513306	19.011780255407725
7	81.71596597633818	2.747613323938603	10.183957115817686	5.352463583905537
8	82.94902437564186	3.691115992686847	7.8518533869385605	5.508006244732734
9	76.29937414514859	6.148252534044386	10.88844678391659	6.663926536890437
10	39.796125134128204	27.22330445835233	17.692142965695417	15.288427441824052
11	32.098778224488015	23.81541196354476	24.690756782396853	19.395053029570374
12	27.801725130443995	21.103949931611886	30.085703890984284	21.00862104695984
13	26.281528762152128	22.302699142961092	31.353762267261665	20.06200982762511
14	21.89720598868027	24.097599277895213	32.32386031324979	21.681334420174725
15	18.57128712415321	22.566465416799065	36.25329851756676	22.60894894148096
16	22.39722210709072	23.261053775622518	30.958746079772315	23.382978037514448
17	23.116333476096397	23.025609851572465	28.819487618758146	25.03856905357299
18	24.378289882704024	21.889146783456063	30.88241389314875	22.85014944069116
19	24.91629939717145	23.628438402343157	28.65531009519073	22.79995210529467
20	25.8212330123467	25.78577250936019	28.260524170707786	20.132470307585322
21	24.191431453005624	25.974588174613046	29.927052679570604	19.906927692810726
22	23.454589832506688	22.942254643253523	29.787743560695024	23.81541196354476
23	22.313176109752558	24.208701178486066	28.9927605310786	24.485362180682777
24	22.325495180595276	23.95782963300682	29.254799832368533	24.461875354029374
25	22.203686050206546	25.59476934554648	26.978765145549243	25.222779458697726
26	20.947601350262268	25.986446719442945	27.19843605366049	25.86751587663429
27	21.396153686740995	25.858075093371646	28.92851715229135	23.817254067596007
28	19.661352196478816	26.072219689329152	29.387316192555136	24.87911192163689
29	21.94820924459918	25.37947343455695	27.968435547081878	24.70388177376199
30	24.284112313084005	24.82603629866033	27.860327065574296	23.029524322681365
31	23.7040797999475	27.479817447488518	25.967795415924066	22.848307336639913
32	24.906628350902402	26.278189948559245	25.72509820717223	23.090083493366123
33	24.514490450993122	24.528306231377478	25.046858521803607	25.91034479582579
34	21.21585775272516	26.382383958957924	28.01955393450399	24.382204353812924
35	21.810627098271645	26.305591246321548	27.19267947850034	24.691102176906462
36	24.075609160783447	26.91659413381965	25.648075231529454	23.359721473867452
37	22.794656056147332	28.0510999663816	26.502696379805013	22.651547597666056
38	22.199426184588038	26.97070594032504	26.44892996780923	24.38093790727769
39	22.005659864697456	23.907286903100722	27.030804584996982	27.05624864720484
40	22.89159678184422	23.86480337841883	28.327760968578307	24.915838871158638
41	20.34016753936346	24.751546216088016	27.239422868800744	27.668863375747776
42	21.725890311914267	23.279820210644598	28.823747484376653	26.17054199306448
43	22.642912734925833	22.795577108172953	28.101872959294106	26.459637197607105
44	22.634508135192018	23.28580704881115	27.61095222963669	26.46873258636014
45	21.818801434999056	23.552451610229202	26.303979405276706	28.324767549495032
46	22.33228793928425	24.268109034138792	28.016560515420714	25.383042511156244
47	19.747470560874632	25.61249959703974	30.464831931031622	24.175197911054006
48	20.540150960427	25.06113482820077	29.095112437426028	25.3036017739462
49	22.97552764767918	22.318587290403098	29.558286474811528	25.14759858710619
50	21.880627052219044	23.017665777851462	28.14723477155607	26.954472398373426
51	20.08365455022727	23.24447483916129	26.405870785611324	30.26599982500012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	540.0
2	932.0
3	1774.0
4	2616.0
5	1803.0
6	990.0
7	1070.5
8	1151.0
9	1234.0
10	1317.0
11	1271.5
12	1226.0
13	1294.5
14	1363.0
15	1300.5
16	1238.0
17	1318.0
18	1398.0
19	1509.0
20	1620.0
21	1901.0
22	2182.0
23	2522.0
24	2862.0
25	3653.0
26	5281.5
27	6119.0
28	7796.5
29	9474.0
30	10414.5
31	11355.0
32	13388.5
33	15422.0
34	17387.5
35	19353.0
36	21665.0
37	23977.0
38	26328.5
39	28680.0
40	32280.5
41	35881.0
42	40718.0
43	45555.0
44	51044.5
45	56534.0
46	66659.0
47	76784.0
48	89550.0
49	102316.0
50	99286.0
51	96256.0
52	81953.5
53	67651.0
54	59889.0
55	52127.0
56	48031.0
57	43935.0
58	40933.0
59	37931.0
60	34446.0
61	30961.0
62	27072.0
63	23183.0
64	20722.5
65	18262.0
66	15957.5
67	13653.0
68	11848.5
69	10044.0
70	9154.5
71	8265.0
72	6550.5
73	4836.0
74	4134.5
75	2501.0
76	1569.0
77	1198.0
78	827.0
79	640.0
80	453.0
81	298.5
82	144.0
83	102.5
84	61.0
85	44.0
86	27.0
87	19.0
88	11.0
89	7.0
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	868572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.11994461282895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6829665107704	20.988470917620187
2	12.276680606912388	7.395458798191848
3	4.959973539000821	4.481823848274057
4	2.4306625306513836	2.9284568318279325
5	1.534757022885277	2.31133982617274
6	0.9533856787473285	1.7229554303120318
7	0.7101005284288147	1.4971732010071526
8	0.5621149684580498	1.3544697372798837
9	0.42834693839692556	1.1611607453631225
>10	6.058774207315673	44.05588989944415
>50	0.33848225284179767	6.2170121547854205
>100	0.05757289165500215	3.0473471804510015
>500	0.0038639524600672507	0.833295364040221
>1k	0.00231837147604035	2.0051460652303024
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	4201	0.4836674449556283	Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	4066	0.468124692023229	Illumina PCR Primer Index 7 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3258	0.3750984374352385	Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3005	0.34597016712489004	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1417	0.16314134003859207	No Hit
TCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1282	0.1475985871061927	Illumina PCR Primer Index 7 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	998	0.1149012401965525	Illumina PCR Primer Index 7 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5078450606282496	0.0
2	0.0	0.0	0.0	1.7985843430366164	0.0
3	0.0	0.0	0.0	2.5422187222245247	0.0
4	0.0	0.0	0.0	3.3163629497612175	0.0
5	0.0	0.0	0.0	4.651658123909129	0.0
6	0.0	0.0	0.0	5.581114749266613	0.0
7	0.0	0.0	0.0	6.4694694279806395	0.0
8	0.0	0.0	0.0	7.869007980915802	0.0
9	0.0	0.0	0.0	8.619089724283077	0.0
10	0.0	0.0	0.0	9.972805938943461	0.0
11	0.0	0.0	0.0	11.999005263812327	0.0
12	0.0	0.0	0.0	14.042934840174448	0.0
13	0.0	0.0	0.0	14.670286401127367	0.0
14	0.0	0.0	0.0	14.903657958119764	0.0
15	0.0	0.0	0.0	15.2591840400105	0.0
16	0.0	0.0	0.0	16.03125590048954	0.0
17	0.0	0.0	0.0	17.06444601023289	0.0
18	0.0	0.0	0.0	18.25502088485468	0.0
19	0.0	0.0	0.0	19.037224317615582	0.0
20	0.0	0.0	0.0	19.92362176077516	0.0
21	0.0	0.0	0.0	21.065841404051707	0.0
22	0.0	0.0	0.0	22.243751813321175	0.0
23	1.1513150320295842E-4	0.0	0.0	23.29732019913145	0.0
24	1.1513150320295842E-4	0.0	0.0	24.114523608866048	0.0
25	1.1513150320295842E-4	0.0	0.0	24.845723785708035	0.0
26	1.1513150320295842E-4	0.0	0.0	25.525920706631116	0.0
27	1.1513150320295842E-4	0.0	0.0	26.23052550623322	0.0
28	1.1513150320295842E-4	0.0	0.0	26.93098557172002	0.0
29	1.1513150320295842E-4	0.0	0.0	27.701445591154215	0.0
30	1.1513150320295842E-4	0.0	0.0	28.531543729247545	0.0
31	1.1513150320295842E-4	0.0	0.0	29.250539966750022	0.0
32	1.1513150320295842E-4	0.0	0.0	29.97701975196069	0.0
33	1.1513150320295842E-4	0.0	0.0	30.66044035497345	0.0
34	1.1513150320295842E-4	0.0	0.0	31.360670157453843	0.0
35	1.1513150320295842E-4	0.0	0.0	32.13205122891367	0.0
36	1.1513150320295842E-4	0.0	0.0	32.816853409964864	0.0
37	1.1513150320295842E-4	0.0	0.0	33.50867861271144	0.0
38	1.1513150320295842E-4	0.0	0.0	34.29134257148515	0.0
39	1.1513150320295842E-4	0.0	0.0	35.49849638256817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTTC	30	2.1648866E-6	45.000004	13
ATGATCG	25	3.8900158E-5	45.0	27
GTCGAAC	20	7.0323347E-4	45.0	45
ATTACCG	25	3.8900158E-5	45.0	8
ATCGTGA	25	3.8900158E-5	45.0	30
TTATACG	20	7.0323347E-4	45.0	1
AACGGTA	20	7.0323347E-4	45.0	25
ATCCCGC	20	7.0323347E-4	45.0	21
AATTCGT	20	7.0323347E-4	45.0	31
GCGATAT	20	7.0323347E-4	45.0	9
ATGCGAG	20	7.0323347E-4	45.0	42
AGGGCGT	25	3.8900158E-5	45.0	10
TAGCCGT	55	6.002665E-11	40.909092	44
CTATCGG	50	1.0804797E-9	40.5	2
TCTAGCG	50	1.0804797E-9	40.5	1
TGCGTTG	150	0.0	40.5	1
TTATCCG	45	1.9274012E-8	40.000004	1
GCGATGT	270	0.0	39.166668	9
CGGGTAC	115	0.0	39.130432	6
CGTTCGG	75	0.0	39.0	2
>>END_MODULE