##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933861.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691393
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.40823670473956	30.0	16.0	31.0	16.0	34.0
2	26.347453618998166	30.0	16.0	33.0	16.0	34.0
3	27.344475573226806	31.0	25.0	33.0	16.0	34.0
4	31.512410452521216	35.0	28.0	35.0	19.0	37.0
5	33.42726929546582	35.0	33.0	35.0	28.0	37.0
6	31.87958657377208	35.0	30.0	37.0	17.0	37.0
7	33.256119168114225	35.0	32.0	35.0	28.0	37.0
8	32.4924565334043	35.0	32.0	37.0	25.0	37.0
9	34.383188721899124	35.0	32.0	39.0	28.0	39.0
10	33.518955210712285	35.0	32.0	37.0	27.0	39.0
11	34.27784631895319	35.0	32.0	39.0	27.0	39.0
12	34.417091292506576	37.0	33.0	39.0	27.0	39.0
13	34.70692211231528	37.0	34.0	39.0	29.0	39.0
14	34.55869816443036	37.0	33.0	39.0	27.0	40.0
15	35.07955533249541	37.0	33.0	39.0	27.0	40.0
16	34.65944838897703	36.0	32.0	39.0	27.0	40.0
17	34.13108174366822	36.0	32.0	38.0	27.0	40.0
18	34.47448846025343	36.0	32.0	39.0	27.0	40.0
19	34.240236739452094	36.0	32.0	39.0	27.0	40.0
20	34.353322929216816	36.0	32.0	39.0	27.0	40.0
21	34.086231709028006	36.0	32.0	39.0	25.0	40.0
22	35.259221600450104	37.0	34.0	39.0	28.0	40.0
23	35.70186276112139	37.0	34.0	39.0	30.0	40.0
24	35.96364151792107	38.0	34.0	39.0	30.0	40.0
25	33.93760712069691	36.0	32.0	39.0	24.0	40.0
26	34.8977195314387	37.0	33.0	39.0	27.0	40.0
27	35.25252063587569	37.0	34.0	39.0	29.0	40.0
28	35.446200641313986	38.0	34.0	39.0	28.0	40.0
29	35.970589809269114	38.0	34.0	40.0	30.0	40.0
30	34.161784976127905	37.0	33.0	39.0	24.0	40.0
31	34.75488615013458	37.0	33.0	39.0	26.0	40.0
32	35.27335393907662	37.0	34.0	39.0	29.0	40.0
33	35.59957506078309	38.0	34.0	39.0	30.0	40.0
34	35.791713251363554	38.0	35.0	40.0	30.0	40.0
35	35.952997788522595	38.0	35.0	40.0	30.0	41.0
36	35.830111094558376	38.0	35.0	40.0	30.0	41.0
37	36.1813917699485	38.0	35.0	40.0	30.0	41.0
38	35.71537605963613	38.0	35.0	40.0	30.0	41.0
39	35.67964095673517	38.0	35.0	40.0	30.0	41.0
40	35.23473624986079	37.0	34.0	40.0	28.0	41.0
41	34.53919840090947	37.0	33.0	39.0	26.0	40.0
42	35.61813903235931	38.0	35.0	40.0	30.0	40.0
43	35.66247271812124	38.0	35.0	40.0	30.0	40.0
44	36.255137092796716	38.0	35.0	40.0	31.0	41.0
45	36.52061851942383	39.0	35.0	40.0	31.0	41.0
46	36.25895981012246	38.0	35.0	40.0	31.0	41.0
47	35.77245502919468	38.0	35.0	40.0	29.0	41.0
48	36.05579460596216	38.0	35.0	40.0	30.0	41.0
49	36.37893643701918	39.0	35.0	40.0	31.0	41.0
50	36.00656645352209	38.0	35.0	40.0	30.0	41.0
51	35.58518960996134	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	13.0
12	6.0
13	14.0
14	22.0
15	39.0
16	147.0
17	378.0
18	894.0
19	1542.0
20	2242.0
21	2794.0
22	3079.0
23	3407.0
24	4225.0
25	5359.0
26	7418.0
27	9748.0
28	13256.0
29	18036.0
30	24898.0
31	34111.0
32	47145.0
33	61413.0
34	77601.0
35	95945.0
36	107711.0
37	103930.0
38	59723.0
39	6283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1907757237924	3.653638379329846	39.90176354114085	13.253822355736897
2	15.491189526072727	28.84379795572127	37.816119052405796	17.848893465800202
3	14.782186108334914	28.833094925751347	40.46482969888327	15.919889267030474
4	15.705394761011465	3.1325165282263487	61.98659807085116	19.17549063991102
5	34.378566170036436	3.4923697520802204	40.48565721666259	21.643406861220754
6	16.385326435182307	24.651247553851427	42.29418001050054	16.669246000465726
7	60.00885169505621	1.4227798083000551	32.995127228652876	5.573241267990854
8	56.561608231497864	20.072520259823285	13.554953550296286	9.81091795838257
9	53.5737272434057	3.3007276614024152	14.37749586703944	28.74804922815244
10	39.75380138358358	19.19096664270538	22.864275455493473	18.19095651821757
11	30.162150904044445	21.860215535881906	28.52502122526551	19.452612334808133
12	20.923121871352475	17.620947854548717	31.81171923927491	29.644211034823897
13	22.098430270482925	20.907935139638383	40.98031076392154	16.013323825957162
14	19.200946494974637	28.91640499686864	26.06751876284544	25.81512974531128
15	11.937783576055876	24.208084258880262	42.029786243135234	21.82434592192863
16	13.194087877661476	25.906828677756355	24.98810372682396	35.91097971775821
17	14.635091764018437	29.018228417123108	34.283251349088	22.06342846977045
18	13.370687872165323	24.870659668235	33.783390922384235	27.975261537215445
19	14.399335833599705	27.75729577823322	29.35045625281135	28.492912135355724
20	16.807806847914282	26.31990778037961	41.15720002950565	15.715085342200455
21	20.75505537371654	33.443352767528744	28.129298387458363	17.672293471296356
22	15.853935460729282	22.025100051634887	34.43410621744796	27.68685827018787
23	17.311138527581274	31.403847016096485	28.945910647056017	22.33910380926622
24	21.297149378139498	22.618395037265344	25.982328429706403	30.102127154888752
25	13.532535041575485	36.251741050314365	23.599023999375174	26.616699908734976
26	15.216526635357894	21.881476960281635	31.67532792492837	31.226668479432103
27	19.261548786290867	27.204498743840333	27.855937216604737	25.678015253264064
28	11.46815197724015	24.760158115572477	38.14675589715256	25.62493401003481
29	18.656104415289136	17.964891168987826	28.70567101489312	34.67333340082992
30	14.40815860154789	27.395273021277333	38.64864122141821	19.547927155756568
31	19.38796024836815	23.115073482086164	27.81428218104609	29.682684088499595
32	19.736821171171822	28.303005671159532	23.85965724269699	28.10051591497166
33	18.112130148844436	20.160747939305143	29.304896057669083	32.42222585418134
34	20.503823440503446	24.898429691940763	25.917531707726287	28.6802151598295
35	15.904847170856517	19.126459191805527	29.9809225722563	34.98777106508165
36	20.61186618898369	20.655546122104216	30.70945178791223	28.02313590099987
37	14.434771540932582	20.143391674489038	39.34853260012757	26.073304184450812
38	18.250690996292988	21.0679020470268	34.47330244882433	26.20810450785588
39	19.946253433286135	26.34955806610712	27.45891265893638	26.245275841670367
40	16.476880732087253	19.877262280642125	35.48647440746435	28.159382579806273
41	22.392474323575737	28.873882148069185	23.412733423682333	25.320910104672738
42	18.003508858203656	17.547473000160547	32.1517573941304	32.2972607475054
43	28.254552765214573	17.44376931788433	25.089493240458033	29.212184676443066
44	19.768351718921075	17.974726385716952	34.77052848380009	27.486393411561878
45	17.076105774863212	15.825442259322845	31.834860925696383	35.26359104011756
46	27.283035842133202	21.977081052310336	27.131313160532432	23.60856994502403
47	13.011268554931855	17.79913883999404	46.24866031330951	22.940932291764597
48	19.519289318809996	24.59961266602352	27.587204383035406	28.293893632131073
49	17.75140911174976	15.268016887645667	40.275646412387744	26.704927588216833
50	25.38006604058762	15.330499440983639	30.862042282753805	28.427392235674937
51	18.982402193831874	15.797238328996677	28.2648218885641	36.95553758860734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	239.5
2	388.0
3	1821.5
4	3255.0
5	2309.5
6	1364.0
7	1472.5
8	1581.0
9	1748.5
10	1916.0
11	2059.5
12	2203.0
13	2110.0
14	2017.0
15	1929.5
16	1842.0
17	1756.5
18	1671.0
19	1741.0
20	1811.0
21	2089.5
22	2368.0
23	2817.5
24	3267.0
25	3443.0
26	4202.0
27	4785.0
28	5783.5
29	6782.0
30	7397.0
31	8012.0
32	9051.0
33	10090.0
34	11568.5
35	13047.0
36	14278.0
37	15509.0
38	17133.5
39	18758.0
40	20846.5
41	22935.0
42	25089.0
43	27243.0
44	32997.5
45	38752.0
46	112565.0
47	186378.0
48	142816.5
49	99255.0
50	89658.5
51	80062.0
52	59946.0
53	39830.0
54	33375.0
55	26920.0
56	23099.5
57	19279.0
58	16770.0
59	14261.0
60	12392.5
61	10524.0
62	9122.0
63	7720.0
64	6599.5
65	5479.0
66	4329.0
67	3179.0
68	2582.5
69	1986.0
70	1712.5
71	1439.0
72	1172.5
73	906.0
74	652.5
75	344.5
76	290.0
77	178.0
78	66.0
79	59.0
80	52.0
81	48.0
82	44.0
83	29.0
84	14.0
85	8.0
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	691393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.464986171589818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92466851751406	20.757996809188093
2	11.338998711534348	6.455288830470001
3	4.336004823812965	3.702729520493484
4	2.039833425518012	2.3225532099886763
5	1.2665624499145274	1.8026341311135972
6	0.8847893343801585	1.5111309700740825
7	0.7075513137370042	1.4098306852849825
8	0.5844461358163763	1.3309000939241806
9	0.5636557148055671	1.444000691473024
>10	5.177246266491168	25.14263711630253
>50	0.08554499671046976	1.701152157237047
>100	0.07369237668430446	4.282438223538011
>500	0.006183975665815759	1.1767390364191932
>1k	0.006183975665815759	4.491323337955978
>5k	0.002061325221938586	4.097170035679941
>10k+	0.002576656527423233	18.371475150857165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	39325	5.687792615777134	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	33613	4.861634410530624	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	25103	3.6307859639886435	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	14052	2.0324186099656782	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTACGT	13148	1.9016680816843676	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTA	8158	1.1799367364147453	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	7223	1.044702506389275	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTC	6481	0.9373829356097039	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT	6069	0.8777930930744163	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	4888	0.7069785201759347	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	4885	0.7065446135555321	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	4057	0.5867863863244205	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGT	2797	0.4045456057553374	No Hit
TGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCTT	2417	0.34958410050434413	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATG	2363	0.3417737813370977	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTAT	1917	0.27726633043724774	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1810	0.26179032764288906	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTTCTACG	1653	0.23908254784182079	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1408	0.20364684050894355	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT	1333	0.19279917499887908	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1090	0.15765273874627023	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	947	0.13696985650708063	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	904	0.13075052828131034	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTTCTACGTCG	812	0.11744405858896459	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC	737	0.10659639307890013	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	693	0.10023242931299564	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8215298679622154	0.0
2	0.0	0.0	0.0	4.392292082795168	0.0
3	0.0	0.0	0.0	5.382756261634121	0.0
4	0.0	0.0	0.0	7.699528343503623	0.0
5	0.0	0.0	0.0	13.40467722409686	0.0
6	0.0	0.0	0.0	14.482645904717	0.0
7	0.0	0.0	0.0	16.275981966840856	0.0
8	0.0	0.0	0.0	18.482686402668236	0.0
9	0.0	0.0	0.0	19.014655919281797	0.0
10	0.0	0.0	0.0	25.25539020499195	0.0
11	0.0	0.0	0.0	27.279419953629844	0.0
12	0.0	0.0	0.0	35.03824886858849	0.0
13	1.4463554013419285E-4	0.0	0.0	35.94598151847068	0.0
14	1.4463554013419285E-4	0.0	0.0	36.41821655700882	0.0
15	1.4463554013419285E-4	0.0	0.0	38.04290757933621	0.0
16	1.4463554013419285E-4	0.0	0.0	39.10858223904494	0.0
17	1.4463554013419285E-4	0.0	0.0	40.149090314770326	0.0
18	1.4463554013419285E-4	0.0	0.0	41.06998479880473	0.0
19	1.4463554013419285E-4	0.0	0.0	43.95531918894174	0.0
20	1.4463554013419285E-4	0.0	0.0	44.907744220725405	0.0
21	1.4463554013419285E-4	0.0	0.0	45.83427949082505	0.0
22	1.4463554013419285E-4	0.0	0.0	46.87999444599526	0.0
23	1.4463554013419285E-4	0.0	0.0	47.78309875859316	0.0
24	1.4463554013419285E-4	0.0	0.0	48.496441242535	0.0
25	1.4463554013419285E-4	0.0	0.0	49.11128692364545	0.0
26	1.4463554013419285E-4	0.0	0.0	49.701689198473225	0.0
27	1.4463554013419285E-4	0.0	0.0	50.29426100640301	0.0
28	1.4463554013419285E-4	0.0	0.0	50.85819497738623	0.0
29	1.4463554013419285E-4	0.0	0.0	51.438906671025016	0.0
30	1.4463554013419285E-4	0.0	0.0	52.08383654448338	0.0
31	1.4463554013419285E-4	0.0	0.0	52.67626371687304	0.0
32	1.4463554013419285E-4	0.0	0.0	53.20577442930432	0.0
33	1.4463554013419285E-4	0.0	0.0	53.73774394591788	0.0
34	1.4463554013419285E-4	0.0	0.0	54.246137869489566	0.0
35	1.4463554013419285E-4	0.0	0.0	54.76653654289239	0.0
36	1.4463554013419285E-4	0.0	0.0	55.28216224347079	0.0
37	1.4463554013419285E-4	0.0	0.0	55.800246748231466	0.0
38	1.4463554013419285E-4	0.0	0.0	56.34132830387348	0.0
39	1.4463554013419285E-4	0.0	0.0	56.88327767275631	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	20	7.0313335E-4	45.0	1
ATAACCG	20	7.0313335E-4	45.0	13
CTTACGA	20	7.0313335E-4	45.0	24
TACGGCT	4280	0.0	44.106308	7
ACGGCTG	4310	0.0	43.85151	8
TGATACC	3860	0.0	43.251293	4
GATACCT	3885	0.0	43.030888	5
TTTCGCG	110	0.0	42.954544	1
GGCGACT	880	0.0	42.954544	10
TTTCTCG	200	0.0	42.75	1
CGGCTGT	4505	0.0	42.60267	9
CGTTTTT	1195	0.0	42.552303	1
CGGGCCA	80	0.0	42.1875	6
CCGGTGA	160	0.0	42.1875	18
ATGATAC	13255	0.0	42.148247	3
GAATCTG	2920	0.0	42.071915	1
TACGGCC	305	0.0	42.049183	7
ACGGCCT	300	0.0	42.0	8
AATGATA	13230	0.0	41.92177	2
GAATGAT	13945	0.0	41.91825	1
>>END_MODULE