SRR2933860.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933860.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647796
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	34674	5.352611007168924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	30495	4.707500509419632	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	23230	3.58600547085811	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTCTGCTT	10972	1.6937430919610494	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	10056	1.5523405516551507	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTA	7032	1.0855269251431006	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT	5834	0.9005921617299273	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC	5356	0.8268034998672421	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	5076	0.7835800159309412	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	3245	0.5009293049046305	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	3183	0.4913583906044495	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	2540	0.3920987471364442	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGT	2402	0.37079574433926726	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATG	2076	0.32047125947057403	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.26196518657108103	No Hit
TGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCTT	1658	0.25594477273709626	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTAT	1491	0.23016505196080247	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTCTGCT	1378	0.2127212888007953	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT	1357	0.20947952750557272	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1131	0.17459200118555843	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	782	0.12071701585066905	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	651	0.10049460015189968	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTGA	95	0.0	45.000004	18
TCGTTGA	20	7.031004E-4	45.0	24
GCGACGA	25	3.8889135E-5	45.0	33
ACGCCCA	20	7.031004E-4	45.0	19
CTCGTAC	20	7.031004E-4	45.0	29
GGTCGTC	20	7.031004E-4	45.0	9
GATCGAG	20	7.031004E-4	45.0	36
TAGGTAA	25	3.8889135E-5	45.0	40
GGCCGTT	20	7.031004E-4	45.0	39
CGTTGAT	20	7.031004E-4	45.0	25
TAGCCCG	20	7.031004E-4	45.0	30
CTACGGC	160	0.0	45.0	6
CGAGGGT	20	7.031004E-4	45.0	4
ATCGGGA	20	7.031004E-4	45.0	38
CCGTTTG	25	3.8889135E-5	45.0	1
TCGAATA	40	6.8066583E-9	45.0	44
CCGTTCG	20	7.031004E-4	45.0	1
CTTACCG	20	7.031004E-4	45.0	1
ACGGCTG	4030	0.0	43.995037	8
TACGGCT	4040	0.0	43.941833	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content