##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933860.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647796
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.50245447640924	30.0	16.0	31.0	16.0	34.0
2	26.474572241878615	30.0	16.0	33.0	16.0	34.0
3	27.536704456341194	31.0	25.0	33.0	16.0	34.0
4	31.53203786377193	35.0	28.0	35.0	19.0	37.0
5	33.35146867223632	35.0	32.0	37.0	28.0	37.0
6	31.983394463689187	35.0	31.0	37.0	17.0	37.0
7	33.178545406269876	35.0	32.0	35.0	28.0	37.0
8	32.38967051355674	35.0	32.0	37.0	19.0	37.0
9	34.31942926476854	35.0	32.0	39.0	28.0	39.0
10	33.26615014603363	35.0	31.0	37.0	27.0	39.0
11	33.787533421015254	35.0	32.0	39.0	27.0	39.0
12	33.877317859326084	35.0	32.0	39.0	26.0	39.0
13	34.02437650124422	35.0	32.0	38.0	27.0	39.0
14	34.04894133338273	36.0	32.0	39.0	25.0	40.0
15	34.56222483621387	36.0	32.0	39.0	27.0	40.0
16	34.467154783295975	36.0	32.0	39.0	27.0	40.0
17	33.913289060136215	36.0	32.0	38.0	25.0	40.0
18	34.219079772026994	36.0	32.0	39.0	27.0	40.0
19	34.00254092337711	36.0	32.0	39.0	25.0	40.0
20	33.99568382638979	36.0	32.0	39.0	25.0	40.0
21	33.729902623665474	36.0	32.0	39.0	25.0	40.0
22	34.88256796892849	37.0	33.0	39.0	27.0	40.0
23	35.42130547271054	37.0	34.0	39.0	29.0	40.0
24	35.73022834349085	37.0	34.0	39.0	30.0	40.0
25	33.79440904235284	36.0	32.0	39.0	24.0	40.0
26	34.63470290029577	36.0	33.0	39.0	27.0	40.0
27	34.92632557163058	37.0	33.0	39.0	27.0	40.0
28	35.079528123051084	37.0	34.0	39.0	27.0	40.0
29	35.508709532013164	37.0	34.0	40.0	29.0	40.0
30	33.849988267911506	36.0	32.0	39.0	24.0	40.0
31	34.51434093449172	37.0	33.0	39.0	25.0	40.0
32	34.86687321317205	37.0	33.0	39.0	27.0	40.0
33	35.37303873441639	37.0	34.0	40.0	29.0	40.0
34	35.53441052430086	38.0	34.0	40.0	30.0	41.0
35	35.530901703622746	38.0	34.0	40.0	29.0	41.0
36	35.44697404738529	38.0	34.0	40.0	29.0	41.0
37	35.68704808303849	38.0	35.0	40.0	30.0	41.0
38	35.32952194826766	37.0	34.0	40.0	30.0	41.0
39	35.371542893133025	37.0	34.0	40.0	29.0	41.0
40	34.95371382348764	37.0	33.0	40.0	27.0	41.0
41	34.27407856794423	37.0	33.0	39.0	24.0	40.0
42	35.33990639028336	37.0	34.0	40.0	29.0	41.0
43	35.42618046422022	38.0	34.0	40.0	30.0	40.0
44	35.984772984087584	38.0	35.0	40.0	31.0	41.0
45	36.17522800387776	38.0	35.0	40.0	31.0	41.0
46	35.94706512544072	38.0	35.0	40.0	31.0	41.0
47	35.531094665604606	38.0	34.0	40.0	28.0	41.0
48	35.67384176500009	38.0	34.0	40.0	30.0	41.0
49	35.967820116209424	38.0	35.0	40.0	31.0	41.0
50	35.68951490901457	38.0	35.0	40.0	29.0	41.0
51	35.24264428925155	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	10.0
11	11.0
12	23.0
13	17.0
14	29.0
15	53.0
16	153.0
17	390.0
18	1005.0
19	1905.0
20	2579.0
21	3048.0
22	3314.0
23	3701.0
24	4362.0
25	5991.0
26	7887.0
27	10584.0
28	14376.0
29	19331.0
30	26632.0
31	36247.0
32	48991.0
33	61253.0
34	69427.0
35	82611.0
36	93052.0
37	90261.0
38	54435.0
39	6111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52897517119587	3.4106416217451176	40.48033640220069	13.58004680485832
2	15.691977103903081	27.935028928860323	38.20678114715126	18.166212820085338
3	15.95980833472266	27.75796701430697	39.683480601917886	16.59874404905248
4	16.35839060444955	3.366800659466869	60.7455124761499	19.52929625993368
5	34.29104224169337	4.048188009805556	39.112313135616766	22.5484566128843
6	17.155246404732356	23.71873244045965	42.76500626740517	16.361014887402824
7	63.42922771983772	1.5327356142983286	30.30861567530519	4.729420990558756
8	60.14161865772558	19.63287825179532	12.06274814910867	8.162754941370432
9	56.51485961629896	3.841178395667772	12.15259124786198	27.491370740171288
10	42.824747296988555	22.137061667562012	19.431117203564085	15.607073831885346
11	34.43537780412352	18.748494896541505	27.357841048725216	19.45828625060976
12	26.39797096616836	15.451314920129178	29.80892132708445	28.341792786618008
13	26.27401218902247	19.06942926476854	38.921975436711556	15.734583109497436
14	20.133035708772514	27.510203829600677	26.78945223496286	25.567308226663947
15	13.11277006958981	21.219643221014024	43.65818868903173	22.009398020364436
16	18.14537292604462	24.51435328405856	24.278630927020235	33.06164286287658
17	19.300829273413235	26.901370184440783	32.66167126688032	21.13612927526567
18	17.070034393543647	23.196191393586872	32.86729155474872	26.866482658120766
19	18.331542646141685	26.510815133159205	27.68942691835084	27.46821530234827
20	19.02064847575471	27.57503905550513	38.43524813367171	14.969064335068447
21	21.8730896763796	33.06658268961216	28.40662801252246	16.653699621485778
22	17.257439070324608	21.22890539614323	34.99450444275667	26.519151090775487
23	18.10400187713416	30.64884624171807	30.58416538539911	20.66298649574866
24	22.82153640961043	22.62980938443584	25.655144520805933	28.893509685147794
25	17.624375575026708	35.33813114005026	22.17596280310468	24.86153048181835
26	18.595977746080557	20.6497107113968	30.974102958338733	29.78020858418391
27	20.636434927044935	26.205317723480846	28.502182785938786	24.656064563535434
28	13.98233394463689	24.523306720016798	36.25570395618374	25.238655379162577
29	25.33930434889996	19.496569907810485	27.7612087756022	27.402916967687357
30	18.920771353944758	26.037209244885734	36.79985674502467	18.242162656144835
31	25.58907433821759	18.599373876961263	29.091565863327347	26.719985921493805
32	26.314611390005492	24.973911540052733	24.327102976863085	24.384374093078684
33	20.55106854627074	17.57791032979518	30.811088676064685	31.059932447869393
34	32.26586764969219	19.143989774558655	26.998777392882943	21.591365182866213
35	17.476952620886824	19.686444497959236	32.21739559984933	30.619207281304604
36	25.418032837498224	21.214394655107473	34.29320341589019	19.074369091504114
37	17.762999462793843	19.963074795151563	37.58837658769119	24.68554915436341
38	18.896998437779793	21.065736744283694	34.82871150794386	25.208553309992652
39	25.796392691526343	20.829088169732447	27.801190498243272	25.573328640497934
40	20.700807044192928	17.840647364293698	34.430592346973434	27.027953244539948
41	25.04770020191542	20.723345003673995	28.719843901475155	25.509110892935432
42	17.696003062692576	17.378310455760765	34.065817016468145	30.85986946507851
43	23.508172325855668	17.082538329968077	32.08417464757424	27.325114696602014
44	22.137679145903956	16.590408091436192	34.817133789032354	26.4547789736275
45	19.213147348856737	15.23288195666537	31.200100031491395	34.353870662986495
46	27.986742739998395	21.726284200581663	27.370344985149647	22.916628074270296
47	14.033893386189481	17.89760974133832	46.05894448252227	22.00955238994992
48	22.127182014090856	22.71980685277464	28.12582973652199	27.027181396612516
49	20.052763524319385	14.781350919116512	39.33831020876943	25.827575347794674
50	23.751304422997364	15.923531482133264	32.460218957820054	27.864945137049318
51	20.21068361027237	16.009206602078432	28.765691668364735	35.01441811928447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	240.5
2	428.0
3	1765.0
4	3102.0
5	2119.5
6	1137.0
7	1187.0
8	1237.0
9	1357.5
10	1478.0
11	1509.5
12	1541.0
13	1460.5
14	1380.0
15	1338.0
16	1296.0
17	1351.5
18	1407.0
19	1415.0
20	1423.0
21	1628.5
22	1834.0
23	2051.0
24	2268.0
25	2431.5
26	3355.0
27	4115.0
28	4613.0
29	5111.0
30	6230.0
31	7349.0
32	8276.5
33	9204.0
34	10501.5
35	11799.0
36	12761.0
37	13723.0
38	15408.5
39	17094.0
40	18976.0
41	20858.0
42	22802.5
43	24747.0
44	28433.0
45	32119.0
46	96392.5
47	160666.0
48	121074.5
49	81483.0
50	74829.5
51	68176.0
52	53921.0
53	39666.0
54	35020.5
55	30375.0
56	28036.5
57	25698.0
58	23935.5
59	22173.0
60	20053.0
61	17933.0
62	15386.0
63	12839.0
64	10608.5
65	8378.0
66	6936.5
67	5495.0
68	4351.5
69	3208.0
70	2572.5
71	1937.0
72	1558.0
73	1179.0
74	950.5
75	499.5
76	277.0
77	227.0
78	177.0
79	114.0
80	51.0
81	47.0
82	43.0
83	28.0
84	13.0
85	9.0
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	647796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.851446403595187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19468411416594	20.385778216337723
2	11.056068826020994	6.1585501668476645
3	3.8971599895988156	3.25624627729641
4	1.9784745771987478	2.2041351459090626
5	1.1754685809796628	1.6369250091132583
6	0.8907605287089093	1.488538147426458
7	0.660346545441121	1.2874124492706833
8	0.5797296909431845	1.2917048332701526
9	0.4936775991134638	1.2374671673127768
>10	5.89718511679662	30.709108228832644
>50	0.09215569009344746	1.7370403752891912
>100	0.06911676757008542	3.685204947917336
>500	0.0039334745771593335	0.6820454054879072
>1k	0.006181174335536095	3.4678205816431955
>5k	0.0022476997583767617	3.6462353962958374
>10k+	0.002809624697970952	17.125787651749704
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	34674	5.352611007168924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	30495	4.707500509419632	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	23230	3.58600547085811	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTCTGCTT	10972	1.6937430919610494	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	10056	1.5523405516551507	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTA	7032	1.0855269251431006	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT	5834	0.9005921617299273	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC	5356	0.8268034998672421	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	5076	0.7835800159309412	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	3245	0.5009293049046305	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	3183	0.4913583906044495	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	2540	0.3920987471364442	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGT	2402	0.37079574433926726	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATG	2076	0.32047125947057403	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.26196518657108103	No Hit
TGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCTT	1658	0.25594477273709626	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTAT	1491	0.23016505196080247	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTCTGCT	1378	0.2127212888007953	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT	1357	0.20947952750557272	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1131	0.17459200118555843	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	782	0.12071701585066905	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	651	0.10049460015189968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5455421151103125	0.0
2	0.0	0.0	0.0	3.1622609586968737	0.0
3	0.0	0.0	0.0	3.8546085496051226	0.0
4	0.0	0.0	0.0	5.678793941302509	0.0
5	0.0	0.0	0.0	10.83103322650958	0.0
6	0.0	0.0	0.0	11.70167768865507	0.0
7	0.0	0.0	0.0	13.21419088725463	0.0
8	0.0	0.0	0.0	15.031584017190598	0.0
9	0.0	0.0	0.0	15.453630463911479	0.0
10	0.0	0.0	0.0	21.324923278316014	0.0
11	0.0	0.0	0.0	22.995819671625018	0.0
12	0.0	0.0	0.0	30.26338538675756	0.0
13	0.0	0.0	0.0	31.060549926211337	0.0
14	0.0	0.0	0.0	31.46762252314	0.0
15	0.0	0.0	0.0	32.939382151171046	0.0
16	0.0	0.0	0.0	33.82963155067336	0.0
17	0.0	0.0	0.0	34.608426109454214	0.0
18	0.0	0.0	0.0	35.31374074554335	0.0
19	0.0	0.0	0.0	37.814836769600305	0.0
20	0.0	0.0	0.0	38.67591031744561	0.0
21	0.0	0.0	0.0	39.478940901147894	0.0
22	0.0	0.0	0.0	40.39882926106367	0.0
23	0.0	0.0	0.0	41.15863636082965	0.0
24	1.5436958548678905E-4	0.0	0.0	41.75712724376193	0.0
25	1.5436958548678905E-4	0.0	0.0	42.30915288146268	0.0
26	1.5436958548678905E-4	0.0	0.0	42.84496971268733	0.0
27	1.5436958548678905E-4	0.0	0.0	43.419996418625615	0.0
28	1.5436958548678905E-4	0.0	0.0	43.89391104607006	0.0
29	1.5436958548678905E-4	0.0	0.0	44.4508765105064	0.0
30	1.5436958548678905E-4	0.0	0.0	45.063106286547	0.0
31	1.5436958548678905E-4	0.0	0.0	45.596761943574826	0.0
32	1.5436958548678905E-4	0.0	0.0	46.05678330832546	0.0
33	1.5436958548678905E-4	0.0	0.0	46.5506116122977	0.0
34	3.087391709735781E-4	0.0	0.0	47.11575866476483	0.0
35	3.087391709735781E-4	0.0	0.0	47.67627462966736	0.0
36	3.087391709735781E-4	0.0	0.0	48.142625147422955	0.0
37	3.087391709735781E-4	0.0	0.0	48.63243984217254	0.0
38	3.087391709735781E-4	0.0	0.0	49.21997048453525	0.0
39	3.087391709735781E-4	0.0	0.0	50.20469407035548	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTGA	95	0.0	45.000004	18
TCGTTGA	20	7.031004E-4	45.0	24
GCGACGA	25	3.8889135E-5	45.0	33
ACGCCCA	20	7.031004E-4	45.0	19
CTCGTAC	20	7.031004E-4	45.0	29
GGTCGTC	20	7.031004E-4	45.0	9
GATCGAG	20	7.031004E-4	45.0	36
TAGGTAA	25	3.8889135E-5	45.0	40
GGCCGTT	20	7.031004E-4	45.0	39
CGTTGAT	20	7.031004E-4	45.0	25
TAGCCCG	20	7.031004E-4	45.0	30
CTACGGC	160	0.0	45.0	6
CGAGGGT	20	7.031004E-4	45.0	4
ATCGGGA	20	7.031004E-4	45.0	38
CCGTTTG	25	3.8889135E-5	45.0	1
TCGAATA	40	6.8066583E-9	45.0	44
CCGTTCG	20	7.031004E-4	45.0	1
CTTACCG	20	7.031004E-4	45.0	1
ACGGCTG	4030	0.0	43.995037	8
TACGGCT	4040	0.0	43.941833	7
>>END_MODULE