SRR2933857.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933857.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399226
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	27236	6.822200958855386	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	23923	5.992345187938661	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	18580	4.654005500643746	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCGATTT	8810	2.2067701001437783	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	8509	2.131374209094598	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	5513	1.3809220842329908	Illumina Paired End PCR Primer 2 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTA	5355	1.341345503549368	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTC	4425	1.1083947438293096	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT	3989	0.9991834199175406	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	2475	0.6199496024808003	TruSeq Adapter, Index 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2330	0.583629322739501	Illumina Paired End PCR Primer 2 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1901	0.4761713916428289	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGT	1883	0.47166266726115036	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATG	1772	0.44385886690746595	No Hit
TGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCTT	1524	0.3817386643154504	Illumina Paired End PCR Primer 2 (96% over 26bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTAT	1169	0.2928166001212346	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT	1083	0.271274916964326	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCCGATT	1056	0.26451183039180814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.24322063192277055	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	710	0.1778441283884316	TruSeq Adapter, Index 1 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	681	0.17058007244017173	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	544	0.13626367020184055	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	524	0.13125397644441994	TruSeq Adapter, Index 1 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGCCGATTTCG	518	0.12975106831719377	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTG	446	0.11171617079047957	TruSeq Adapter, Index 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGCTTTCGTATGC	427	0.10695696172093	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	419	0.10495308421796176	Illumina Paired End PCR Primer 2 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGG	20	7.0277473E-4	45.000004	10
CTAGATG	20	7.0277473E-4	45.000004	28
CAGAATC	20	7.0277473E-4	45.000004	13
CGGGTCT	20	7.0277473E-4	45.000004	6
CGGGTCC	40	6.7957444E-9	45.000004	6
CGGGTAC	20	7.0277473E-4	45.000004	6
TCGTTCA	20	7.0277473E-4	45.000004	16
CTCCGCG	20	7.0277473E-4	45.000004	1
CCTAGAT	20	7.0277473E-4	45.000004	27
AGCCCCC	40	6.7957444E-9	45.000004	36
GGATAAC	20	7.0277473E-4	45.000004	8
ATCACCG	20	7.0277473E-4	45.000004	14
GACCCCT	20	7.0277473E-4	45.000004	13
ACGGGAT	20	7.0277473E-4	45.000004	5
GCTCTAG	20	7.0277473E-4	45.000004	1
ACTAGCT	20	7.0277473E-4	45.000004	39
TCTCGTT	20	7.0277473E-4	45.000004	25
TATTCAA	20	7.0277473E-4	45.000004	10
GCGGGTT	20	7.0277473E-4	45.000004	5
CCGAGAC	20	7.0277473E-4	45.000004	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content