FastQCFastQC Report
Sat 14 Jan 2017
SRR2933855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388074
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGC294307.583605188701124No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCG231745.971541510124358No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTC179234.618449058684684No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGTTTCT88592.2828120410024892No Hit
GCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC88012.267866437844329Illumina PCR Primer Index 2 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTA57431.479872395470967No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCT52911.3633997639625433Illumina PCR Primer Index 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTC45751.178898869803182No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT39971.0299582038477197No Hit
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT28130.724861753170787Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC22720.5854553512989791Illumina PCR Primer Index 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC20340.5241268417879064Illumina PCR Primer Index 2 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGT20300.5230961105356196No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATG18610.4795477151264965No Hit
TGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCTT14000.36075593830042724Illumina PCR Primer Index 2 (96% over 25bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTAT12500.32210351633966716No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.28473950844426577No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT10550.2718553677906791No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGTTTC10370.2672170771553879No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG7790.2007349113828806Illumina PCR Primer Index 2 (95% over 22bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGC5770.14868298314239037No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCAGTTTCTCG5290.13631420811494713No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGC5280.13605652530187543No Hit
TCCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG4970.12806835809665168Illumina PCR Primer Index 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG4110.10590763617248256Illumina PCR Primer Index 2 (95% over 22bp)
GAATGCTACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCG4040.10410385648098044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAG302.1617725E-645.00000431
GTTTGCG302.1617725E-645.0000041
CTGTCGG302.1617725E-645.0000042
GGCACCG600.045.0000048
ATAGCAA302.1617725E-645.00000410
CATAGGG302.1617725E-645.00000423
CGAGATA302.1617725E-645.00000419
ACCCGCG302.1617725E-645.00000439
ACATAGG302.1617725E-645.00000422
TCGCCTG207.0275046E-445.01
CGGGTTG351.209155E-745.06
CGGGTAG207.0275046E-445.06
TGATAGT253.886014E-545.032
CAAACTG253.886014E-545.026
ACACGCG253.886014E-545.036
ACGCCGG207.0275046E-445.027
CGCGGGT207.0275046E-445.04
CGCGGAT253.886014E-545.042
CGACGAA207.0275046E-445.019
CGTGCGG253.886014E-545.02
Produced by FastQC (version 0.11.5)