##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933855.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388074
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.368182356973154	25.0	16.0	31.0	16.0	33.0
2	25.22712678509769	28.0	16.0	31.0	16.0	34.0
3	26.34076232883419	30.0	16.0	33.0	16.0	34.0
4	30.899392899292405	33.0	28.0	35.0	19.0	37.0
5	33.153560918793836	35.0	32.0	35.0	28.0	37.0
6	31.171722403459135	35.0	28.0	36.0	17.0	37.0
7	33.07730484392152	35.0	32.0	35.0	28.0	37.0
8	31.740273762220607	35.0	30.0	36.0	17.0	37.0
9	34.06710060452388	35.0	32.0	38.0	28.0	39.0
10	33.50849064869071	35.0	32.0	37.0	27.0	39.0
11	34.18029551065003	35.0	32.0	39.0	27.0	39.0
12	34.4034359426295	35.0	33.0	39.0	27.0	39.0
13	34.58236831119838	35.0	33.0	38.0	29.0	39.0
14	34.43102346459696	36.0	32.0	39.0	27.0	40.0
15	34.99678669532099	37.0	33.0	39.0	27.0	40.0
16	34.69825600272113	36.0	32.0	39.0	27.0	40.0
17	34.004161577431105	36.0	32.0	38.0	27.0	40.0
18	34.32980565562238	36.0	32.0	38.0	27.0	40.0
19	34.059117590974914	36.0	32.0	39.0	27.0	40.0
20	33.7913439189433	36.0	31.0	39.0	25.0	40.0
21	33.87337466565655	36.0	32.0	39.0	25.0	40.0
22	35.179981137618086	37.0	33.0	39.0	28.0	40.0
23	35.41732246942593	37.0	33.0	39.0	29.0	40.0
24	35.762751434004855	37.0	34.0	39.0	30.0	40.0
25	33.631503269994894	36.0	32.0	39.0	24.0	40.0
26	34.780178522652896	37.0	33.0	39.0	27.0	40.0
27	34.771917211665816	37.0	33.0	39.0	27.0	40.0
28	34.7829975726279	37.0	33.0	39.0	26.0	40.0
29	35.30206352396708	37.0	34.0	40.0	27.0	40.0
30	33.35826929915429	36.0	31.0	39.0	23.0	40.0
31	34.23358431639326	36.0	32.0	39.0	25.0	40.0
32	34.63931878971536	37.0	33.0	39.0	27.0	40.0
33	34.915858831047686	37.0	33.0	39.0	27.0	40.0
34	35.25124847322933	38.0	34.0	40.0	28.0	40.0
35	35.41608816875132	38.0	34.0	40.0	29.0	41.0
36	35.35241732246943	38.0	34.0	40.0	27.0	41.0
37	35.84783314522488	38.0	35.0	40.0	30.0	41.0
38	34.68642836160114	37.0	33.0	40.0	25.0	40.0
39	34.840844787334376	37.0	33.0	40.0	27.0	40.0
40	34.40692239109036	37.0	33.0	39.0	24.0	40.0
41	33.9915943866376	37.0	33.0	39.0	24.0	40.0
42	35.05107788720708	37.0	34.0	40.0	28.0	40.0
43	34.94959724176317	37.0	34.0	40.0	27.0	40.0
44	35.70723367192855	38.0	35.0	40.0	30.0	41.0
45	35.830857001499716	38.0	35.0	40.0	30.0	41.0
46	35.493766652751795	38.0	35.0	40.0	28.0	41.0
47	34.7063575503641	37.0	33.0	40.0	26.0	41.0
48	35.188958806825504	37.0	34.0	40.0	27.0	41.0
49	35.54921484046857	38.0	34.0	40.0	29.0	41.0
50	35.08277545004303	37.0	34.0	40.0	26.0	41.0
51	34.71459566989801	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	12.0
12	9.0
13	11.0
14	15.0
15	32.0
16	83.0
17	241.0
18	645.0
19	1213.0
20	1796.0
21	2119.0
22	2272.0
23	2347.0
24	2876.0
25	3685.0
26	5118.0
27	7155.0
28	9251.0
29	12561.0
30	16670.0
31	22444.0
32	30074.0
33	38526.0
34	45434.0
35	52294.0
36	54336.0
37	48035.0
38	26273.0
39	2541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.56844828563625	3.345753644923391	33.81107726876833	12.274720800672037
2	14.035982828017337	36.51777753727382	32.5551312378567	16.891108396852147
3	13.794018666542978	36.47654828718234	35.44349788957776	14.285935156696919
4	14.754917876487475	2.9589717425027184	63.903791544911535	18.382318836098268
5	39.02683508815329	3.8871452351871034	35.608672572756745	21.477347103902865
6	14.67555157006138	30.772223854213372	38.63232270134046	15.91990187438478
7	53.65626143467483	1.696583641264295	39.27601436839366	5.3711405556672185
8	48.36809474481671	26.036786798394118	14.684312785705819	10.91080567108335
9	45.18854651432459	3.8098403912655834	15.465091709313173	35.536521385096655
10	39.97098491524812	19.321315006931666	22.639496590856385	18.068203486963828
11	33.493869725877026	19.401196678983908	28.333256028489412	18.771677566649657
12	22.50859372181594	14.481774094631437	31.097934929936045	31.911697253616577
13	23.041997144874433	20.10003246803445	42.191437715487254	14.666532671603868
14	19.402227410236193	30.891530996665583	23.508145353721197	26.198096239377026
15	9.998350829996342	22.728397161366132	45.44468323051789	21.828568778119635
16	11.033462690105496	24.668748743796286	24.541711116951923	39.7560774491463
17	13.013239742935626	29.192370527270572	35.84290624983895	21.951483479954852
18	11.504764555213697	23.823806799734072	33.434602678870526	31.23682596618171
19	13.145173343228352	27.471307018764463	28.639383210418636	30.744136427588554
20	16.521848925720352	24.789086617500786	43.83700015976334	14.852064297015518
21	19.454537021289752	35.80167699974747	26.462221122775553	18.28156485618722
22	13.448466014213784	21.342578992666347	35.95448290789901	29.25447208522086
23	17.28845529460876	33.6062194323763	27.83567051644789	21.269654756567046
24	21.478893200781293	22.658822801836763	24.19667383024887	31.665610167133075
25	13.1593458979473	39.65790029736597	20.427805006261693	26.754948798425044
26	18.332586053175426	21.003726093477017	30.390853290867202	30.272834562480348
27	21.530945129021784	28.895262243798864	21.3266026582559	28.247189968923454
28	14.280781500435483	29.03312254879224	34.234450130645186	22.45164582012709
29	29.874972299097596	16.865855481171117	24.311600364878863	28.947571854852427
30	15.549611672000701	30.58076552410107	30.857516865340116	23.01210593855811
31	24.570571592015956	25.226116668470443	22.573014425083876	27.630297314429725
32	29.22020027108232	29.29750511500384	23.499126455263685	17.98316815865015
33	15.62691651592222	18.334132150053854	27.725639955266264	38.31331137875766
34	30.949767312419795	20.123739286837047	30.514798723954712	18.41169467678845
35	17.922355014765227	16.700680797992135	29.05219107695955	36.32477311028309
36	24.38890520880038	25.936032818483074	22.826574313146462	26.84848765957008
37	20.557934826862915	18.73457124156733	33.134402201641954	27.573091729927796
38	19.221076392646765	28.436329153718106	27.499909811015428	24.842684642619705
39	30.216144343604572	19.515865530800827	32.2634342934595	18.004555832135107
40	17.433273035555075	17.287424563356474	43.8264351644274	21.45286723666105
41	16.532156238243225	22.575333570401522	31.63288444987296	29.259625741482292
42	18.563985219313842	15.817601797595305	42.01930559635533	23.59910738673552
43	25.3915490344625	16.64888655256472	22.898725500806545	35.06083891216624
44	21.00836438411231	16.045651087163787	33.70001597633441	29.245968552389495
45	17.710282059607188	17.029484067471667	29.80823245051202	35.45200142240913
46	33.19676144240532	22.13366522879657	25.410617562629806	19.25895576616831
47	12.751434004854744	16.657905451022227	49.43258244561604	21.158078098506987
48	20.60560614728119	22.57662198446688	28.64530991511928	28.17246195313265
49	17.949669392950828	13.361884589021681	45.501115766580604	23.187330251446888
50	24.79449795657529	13.788865010281546	30.698526569674854	30.718110463468307
51	19.414338502450565	14.5358874853765	27.942866566685737	38.1069074454872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	145.0
2	221.0
3	1025.5
4	1830.0
5	1292.0
6	754.0
7	825.5
8	897.0
9	1007.0
10	1117.0
11	1228.5
12	1340.0
13	1302.5
14	1265.0
15	1182.5
16	1100.0
17	1078.5
18	1057.0
19	1050.0
20	1043.0
21	1067.0
22	1091.0
23	1152.5
24	1214.0
25	1469.0
26	2113.5
27	2503.0
28	2782.0
29	3061.0
30	3475.0
31	3889.0
32	4445.5
33	5002.0
34	5586.0
35	6170.0
36	6631.0
37	7092.0
38	7674.0
39	8256.0
40	9131.5
41	10007.0
42	11544.0
43	13081.0
44	15817.5
45	18554.0
46	69910.5
47	121267.0
48	88235.5
49	55204.0
50	49429.5
51	43655.0
52	32159.5
53	20664.0
54	17522.5
55	14381.0
56	12705.5
57	11030.0
58	9950.5
59	8871.0
60	7981.0
61	7091.0
62	6158.0
63	5225.0
64	4178.5
65	3132.0
66	2517.0
67	1902.0
68	1609.0
69	1316.0
70	1121.5
71	927.0
72	725.0
73	523.0
74	370.5
75	186.0
76	154.0
77	121.0
78	88.0
79	61.0
80	34.0
81	34.5
82	35.0
83	27.5
84	20.0
85	10.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	388074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.251975917537756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84183119799302	19.12241998399063
2	11.090488283034286	5.822944626399015
3	4.23506384182827	3.33536381954732
4	2.2125020638051898	2.3233020358606575
5	1.3600301681164695	1.7851739610259192
6	0.9942881414234529	1.5661217006245116
7	0.7434675482241276	1.3662244520015447
8	0.6635247892761754	1.393506943101398
9	0.5855806113972384	1.3835383297359605
>10	5.045386530017123	21.957178267910653
>50	0.12291197131935605	2.213294062679081
>100	0.08194131421290404	4.398256282431845
>500	0.003997137278678246	0.6330068239000383
>1k	0.011991411836034737	6.670837349753035
>5k	0.003997137278678246	7.527350934516244
>10k+	0.002997852959008684	18.50148042652217
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGC	29430	7.583605188701124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCG	23174	5.971541510124358	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTC	17923	4.618449058684684	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGTTTCT	8859	2.2828120410024892	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	8801	2.267866437844329	Illumina PCR Primer Index 2 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTA	5743	1.479872395470967	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCT	5291	1.3633997639625433	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTC	4575	1.178898869803182	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCT	3997	1.0299582038477197	No Hit
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT	2813	0.724861753170787	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	2272	0.5854553512989791	Illumina PCR Primer Index 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	2034	0.5241268417879064	Illumina PCR Primer Index 2 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGT	2030	0.5230961105356196	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATG	1861	0.4795477151264965	No Hit
TGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCTT	1400	0.36075593830042724	Illumina PCR Primer Index 2 (96% over 25bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTAT	1250	0.32210351633966716	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1105	0.28473950844426577	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT	1055	0.2718553677906791	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGTTTC	1037	0.2672170771553879	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG	779	0.2007349113828806	Illumina PCR Primer Index 2 (95% over 22bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGC	577	0.14868298314239037	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCAGTTTCTCG	529	0.13631420811494713	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGC	528	0.13605652530187543	No Hit
TCCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG	497	0.12806835809665168	Illumina PCR Primer Index 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG	411	0.10590763617248256	Illumina PCR Primer Index 2 (95% over 22bp)
GAATGCTACCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCG	404	0.10410385648098044	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8178852486896829	0.0
2	0.0	0.0	0.0	4.532640681931796	0.0
3	0.0	0.0	0.0	5.405154686992687	0.0
4	0.0	0.0	0.0	8.18194468065369	0.0
5	0.0	0.0	0.0	14.854641125146236	0.0
6	0.0	0.0	0.0	15.944639424439668	0.0
7	0.0	0.0	0.0	17.83525822394698	0.0
8	0.0	0.0	0.0	20.16110329473245	0.0
9	0.0	0.0	0.0	20.645289300494234	0.0
10	0.0	0.0	0.0	28.037951524709204	0.0
11	0.0	0.0	0.0	29.926766544525012	0.0
12	0.0	0.0	0.0	39.77823817107046	0.0
13	0.0	0.0	0.0	40.771090049835855	0.0
14	0.0	0.0	0.0	41.286455675979326	0.0
15	0.0	0.0	0.0	43.25824456160423	0.0
16	0.0	0.0	0.0	44.359323221859746	0.0
17	0.0	0.0	0.0	45.256317094162455	0.0
18	0.0	0.0	0.0	45.96314105041822	0.0
19	0.0	0.0	0.0	49.21973644201879	0.0
20	0.0	0.0	0.0	50.12626457840515	0.0
21	0.0	0.0	0.0	50.91425862077851	0.0
22	0.0	0.0	0.0	51.80661420244593	0.0
23	0.0	0.0	0.0	52.471435860171	0.0
24	0.0	0.0	0.0	53.0311229301628	0.0
25	0.0	0.0	0.0	53.48309858429062	0.0
26	0.0	0.0	0.0	53.96857300411777	0.0
27	0.0	0.0	0.0	54.49553435684947	0.0
28	0.0	0.0	0.0	54.90447698119431	0.0
29	0.0	0.0	0.0	55.347433736864616	0.0
30	0.0	0.0	0.0	55.85738802393358	0.0
31	0.0	0.0	0.0	56.32508232965878	0.0
32	0.0	0.0	0.0	56.77551188690817	0.0
33	0.0	0.0	0.0	57.19347340971052	0.0
34	0.0	0.0	0.0	57.57973994650505	0.0
35	0.0	0.0	0.0	57.98017903801852	0.0
36	0.0	0.0	0.0	58.38113349515814	0.0
37	0.0	0.0	0.0	58.7944567273252	0.0
38	0.0	0.0	0.0	59.20494544854847	0.0
39	0.0	0.0	0.0	59.63321428387369	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATAG	30	2.1617725E-6	45.000004	31
GTTTGCG	30	2.1617725E-6	45.000004	1
CTGTCGG	30	2.1617725E-6	45.000004	2
GGCACCG	60	0.0	45.000004	8
ATAGCAA	30	2.1617725E-6	45.000004	10
CATAGGG	30	2.1617725E-6	45.000004	23
CGAGATA	30	2.1617725E-6	45.000004	19
ACCCGCG	30	2.1617725E-6	45.000004	39
ACATAGG	30	2.1617725E-6	45.000004	22
TCGCCTG	20	7.0275046E-4	45.0	1
CGGGTTG	35	1.209155E-7	45.0	6
CGGGTAG	20	7.0275046E-4	45.0	6
TGATAGT	25	3.886014E-5	45.0	32
CAAACTG	25	3.886014E-5	45.0	26
ACACGCG	25	3.886014E-5	45.0	36
ACGCCGG	20	7.0275046E-4	45.0	27
CGCGGGT	20	7.0275046E-4	45.0	4
CGCGGAT	25	3.886014E-5	45.0	42
CGACGAA	20	7.0275046E-4	45.0	19
CGTGCGG	25	3.886014E-5	45.0	2
>>END_MODULE