SRR2933854.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353031
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	29665	8.402944783885834	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	23902	6.770510238477641	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	18722	5.303216997940691	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCGCTGT	8549	2.4216003693726615	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	8026	2.273454739102232	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTA	5371	1.5213961380162082	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	5257	1.4891043562746615	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTC	3998	1.1324784509009125	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT	3929	1.1129334251099763	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	2597	0.7356294489719033	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	2230	0.6316725726635904	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATG	1985	0.5622735680435996	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGT	1845	0.5226169939750334	No Hit
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1592	0.45095189940826724	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTAT	1180	0.33424826714934386	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	1134	0.32121824995538634	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATCGCTG	1004	0.2843942883202891	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	807	0.22859182338094952	No Hit
TGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCTT	803	0.22745877840756193	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.16910696227810024	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	530	0.15012845897385782	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAATCGCTGTCG	499	0.14134736043010387	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	491	0.13908127048332866	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCGCTGTCGTATGCCGTCTTC	489	0.13851474799663485	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	397	0.1124547136087199	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	391	0.11075514614863852	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1612123E-6	45.000004	6
CACGCCG	30	2.1612123E-6	45.000004	26
GACTTAT	35	1.2087366E-7	45.000004	23
CTTCTTG	65	0.0	45.000004	1
CGACTGA	35	1.2087366E-7	45.000004	41
AACTACT	30	2.1612123E-6	45.000004	35
CAATCTA	30	2.1612123E-6	45.000004	43
GTTTGCG	40	6.7921064E-9	45.0	1
GAGTGCT	25	3.8852966E-5	45.0	9
AGGTAAC	20	7.026639E-4	45.0	14
GATACCG	20	7.026639E-4	45.0	27
ACCGGTG	90	0.0	45.0	17
CGGCCTC	20	7.026639E-4	45.0	6
GCAACGA	20	7.026639E-4	45.0	9
TTTCGCA	20	7.026639E-4	45.0	14
AAGACGC	20	7.026639E-4	45.0	38
ACGGCTA	25	3.8852966E-5	45.0	30
TTCTTCG	25	3.8852966E-5	45.0	1
TGATTGG	25	3.8852966E-5	45.0	2
GCGATCA	20	7.026639E-4	45.0	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content