##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353031
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.967620407273017	25.0	16.0	31.0	16.0	33.0
2	24.832816381564225	28.0	16.0	31.0	16.0	34.0
3	26.00125767992046	30.0	16.0	33.0	16.0	34.0
4	30.61044214247474	32.0	28.0	35.0	19.0	37.0
5	33.037268681787154	35.0	32.0	35.0	28.0	37.0
6	31.03786353039818	35.0	28.0	36.0	17.0	37.0
7	32.97621172078373	35.0	32.0	35.0	28.0	37.0
8	31.456169571510717	35.0	28.0	36.0	17.0	37.0
9	34.057439714925884	35.0	32.0	37.0	28.0	39.0
10	33.39027167585849	35.0	32.0	37.0	27.0	39.0
11	34.028632046477505	35.0	32.0	37.0	27.0	39.0
12	34.27165603020698	35.0	33.0	39.0	27.0	39.0
13	34.412915579651646	35.0	33.0	37.0	27.0	39.0
14	34.3689024476604	36.0	32.0	38.0	27.0	40.0
15	34.930079794692254	37.0	33.0	39.0	27.0	40.0
16	34.594270758092065	36.0	32.0	38.0	27.0	40.0
17	33.981466216847814	36.0	32.0	38.0	27.0	40.0
18	34.395775441816724	36.0	32.0	38.0	27.0	40.0
19	34.042415538578766	36.0	32.0	39.0	27.0	40.0
20	33.662672683135476	35.0	31.0	39.0	25.0	40.0
21	33.85965255175891	36.0	32.0	39.0	25.0	40.0
22	35.12586430086876	37.0	33.0	39.0	27.0	40.0
23	35.293056417141834	37.0	33.0	39.0	28.0	40.0
24	35.691964728309976	37.0	34.0	39.0	30.0	40.0
25	33.68515512801992	36.0	32.0	39.0	24.0	40.0
26	34.77080199755829	37.0	33.0	39.0	27.0	40.0
27	34.74760573434061	37.0	33.0	39.0	27.0	40.0
28	34.88658786338877	37.0	33.0	39.0	27.0	40.0
29	35.30772085170991	37.0	34.0	40.0	27.0	40.0
30	33.39763930079795	36.0	31.0	39.0	23.0	40.0
31	34.151663734912795	36.0	32.0	39.0	25.0	40.0
32	34.70767722947843	37.0	33.0	39.0	27.0	40.0
33	35.11116304233906	37.0	34.0	39.0	27.0	40.0
34	35.27779713396274	38.0	34.0	40.0	28.0	40.0
35	35.42154938234886	38.0	34.0	40.0	29.0	40.0
36	35.42138792344015	38.0	34.0	40.0	28.0	41.0
37	35.63765788273551	38.0	35.0	40.0	30.0	41.0
38	35.358192340049456	37.0	34.0	40.0	30.0	41.0
39	35.23754571128315	37.0	34.0	40.0	29.0	40.0
40	34.85090827717679	37.0	33.0	40.0	26.0	40.0
41	34.39029716937039	37.0	33.0	39.0	25.0	40.0
42	35.241188450872585	37.0	34.0	40.0	30.0	40.0
43	35.239137639470755	38.0	34.0	40.0	29.0	40.0
44	35.89784183258694	38.0	35.0	40.0	30.0	41.0
45	36.12607391418884	38.0	35.0	40.0	31.0	41.0
46	35.78788548314454	38.0	35.0	40.0	30.0	41.0
47	35.045287807586305	37.0	34.0	40.0	26.0	41.0
48	35.5213281553178	37.0	34.0	40.0	29.0	41.0
49	35.80811600114438	38.0	35.0	40.0	30.0	41.0
50	35.39137922731998	38.0	34.0	40.0	28.0	41.0
51	35.03890876438613	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	9.0
11	3.0
12	6.0
13	7.0
14	11.0
15	25.0
16	59.0
17	189.0
18	597.0
19	1194.0
20	1852.0
21	2092.0
22	2252.0
23	2291.0
24	2321.0
25	2938.0
26	4080.0
27	5580.0
28	7711.0
29	10739.0
30	14615.0
31	20419.0
32	27523.0
33	35943.0
34	43204.0
35	49998.0
36	49850.0
37	42241.0
38	22908.0
39	2372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.95985055136801	3.124088252872977	30.546042698799823	12.370018496959192
2	12.835699981021499	39.271055516371085	31.08452232240228	16.808722180205137
3	12.968832765394541	39.479535791474405	33.644920701014925	13.906710742116132
4	14.56500987165433	3.0926462548614713	64.80025833425394	17.542085539230264
5	41.295806883814734	3.713271639034532	33.73584756012928	21.255073917021452
6	14.914837507187753	32.33908636918571	37.34601210658554	15.400064017040997
7	53.270109423818305	1.3055510705858693	40.24037549110418	5.183964014491646
8	47.35306531154488	27.964682988179508	14.31913911242922	10.363112587846393
9	44.34483090720078	3.3099076285085447	13.923989677960291	38.42127178633038
10	41.80029515821557	17.05714229062037	22.25272001608924	18.889842535074823
11	36.09569697845232	18.236642107916868	26.462831875954233	19.20482903767658
12	23.152074463715653	13.729105942537625	30.270146247779937	32.84867334596678
13	22.996847302361548	19.569952780350732	43.20810353764967	14.22509637963805
14	20.245814106976443	31.291019768802176	22.631440298444048	25.83172582577734
15	10.01328495231297	21.344017947432377	46.82138395778274	21.821313142471908
16	12.169186275426238	23.642682937192486	23.12488138435435	41.06324940302693
17	13.127175800425459	28.382210060872843	35.74841869410901	22.74219544459268
18	12.284756862711774	23.32514708340061	33.4690154688965	30.921080584991117
19	13.228016803056954	27.10866751078517	28.01227087706179	31.651044809096085
20	17.769544317637827	24.75929875846597	43.783407122887226	13.687749801008978
21	19.786647631511116	36.77977288113509	26.28862621129589	17.144953276057908
22	14.011517402154485	20.054046245230587	36.2478082661296	29.68662808648532
23	17.3882746840929	34.32758029748096	27.872339822848417	20.411805195577728
24	22.08899501743473	22.26178437587634	22.991465338737957	32.657755267950975
25	12.27682554789806	42.17788239559699	19.94980610767893	25.595485948826024
26	14.152864762584588	24.25367743909175	30.85224810285782	30.741209695465837
27	21.410301078375554	32.70789250802337	20.995889879359037	24.885916534242035
28	10.814914270984701	28.12614189688724	37.90545306219567	23.153490769932386
29	20.315496372839778	25.699726086377684	23.8149057731474	30.169871767635136
30	16.754052760239187	36.44637439771578	29.747529253804906	17.052043588240124
31	22.23487455775838	19.863977950944818	24.914525919820072	32.986621571476725
32	24.954182493888638	31.838563752191735	20.506980973342284	22.700272780577343
33	25.701425653837767	18.245989728947315	19.37393599995468	36.67864861726024
34	23.869291931870006	25.977038843614302	24.02225300327733	26.131416221238364
35	18.003801365885717	26.48719234288207	26.271064014208385	29.237942277023834
36	24.338655812095823	29.8990740189955	26.10875532177061	19.65351484713807
37	14.24039248677878	27.812288439258875	31.612521280000905	26.334797793961435
38	26.525149349490558	20.092853035569114	28.973942798224517	24.40805481671581
39	22.16179315697488	21.15111704071314	22.86059864431169	33.82649115800029
40	26.64723494537307	19.008245734793828	32.52830487974144	21.816214440091663
41	24.229600233407265	23.079276324175495	20.459109823216657	32.23201361920058
42	17.89757839963063	16.28355583503998	42.264843597304484	23.554022168024904
43	33.89957255878379	19.50565247811099	20.417187159201315	26.177587803903908
44	22.226376720457978	19.361472505247416	31.488169594171616	26.92398118012299
45	17.2101033620277	14.941180802819016	31.402341437437503	36.44637439771578
46	32.18159311788483	23.350357334058483	23.604442669340653	20.863606878716034
47	15.805410856270413	17.106429746962732	47.12617305562401	19.961986341142847
48	21.834059898422517	23.602459840637223	23.540425628344252	31.023054632596004
49	18.083114514022846	13.539320909495201	44.17090850378579	24.206656072696163
50	29.033710920570716	14.368143307528234	28.928904260532363	27.669241511368693
51	20.526809260376567	15.54962595352816	25.573958094331662	38.34960669176361
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	125.5
2	209.0
3	663.5
4	1118.0
5	857.5
6	597.0
7	692.0
8	787.0
9	821.5
10	856.0
11	957.5
12	1059.0
13	963.0
14	867.0
15	789.5
16	712.0
17	744.0
18	776.0
19	798.5
20	821.0
21	888.0
22	955.0
23	1117.0
24	1279.0
25	1322.5
26	1761.0
27	2156.0
28	2199.0
29	2242.0
30	2672.5
31	3103.0
32	3598.0
33	4093.0
34	4652.0
35	5211.0
36	5592.5
37	5974.0
38	6627.5
39	7281.0
40	7890.0
41	8499.0
42	9724.5
43	10950.0
44	13496.5
45	16043.0
46	66567.0
47	117091.0
48	83036.5
49	48982.0
50	43989.0
51	38996.0
52	28719.0
53	18442.0
54	15443.5
55	12445.0
56	11292.0
57	10139.0
58	9199.0
59	8259.0
60	7557.0
61	6855.0
62	5814.0
63	4773.0
64	4006.0
65	3239.0
66	2980.0
67	2721.0
68	2124.5
69	1528.0
70	1192.5
71	857.0
72	715.5
73	574.0
74	600.5
75	457.0
76	287.0
77	217.0
78	147.0
79	101.5
80	56.0
81	33.0
82	10.0
83	8.0
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.00649801292238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76337258062759	19.44333500457467
2	10.566272015335853	5.495834643416584
3	3.936347496487349	3.071118400367107
4	1.9398547015063554	2.0179530976033266
5	1.0957292699131913	1.4248040540349147
6	0.8277875200139417	1.291671269661871
7	0.6458921044319308	1.1758174211329884
8	0.6121270871682042	1.2735425500876694
9	0.487958959166113	1.1421093331747072
>10	4.939495267451612	23.087490900232556
>50	0.09149230484364619	1.6426319501686821
>100	0.07079761684329765	3.887760564936224
>500	0.004356776421126009	0.7752860230404696
>1k	0.010891941052815022	6.088417164498302
>5k	0.004356776421126009	7.705555602765762
>10k+	0.0032675823158445066	20.476672020304164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	29665	8.402944783885834	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	23902	6.770510238477641	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	18722	5.303216997940691	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCGCTGT	8549	2.4216003693726615	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	8026	2.273454739102232	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTA	5371	1.5213961380162082	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	5257	1.4891043562746615	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTC	3998	1.1324784509009125	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT	3929	1.1129334251099763	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	2597	0.7356294489719033	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	2230	0.6316725726635904	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATG	1985	0.5622735680435996	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGT	1845	0.5226169939750334	No Hit
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1592	0.45095189940826724	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTAT	1180	0.33424826714934386	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	1134	0.32121824995538634	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATCGCTG	1004	0.2843942883202891	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	807	0.22859182338094952	No Hit
TGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCTT	803	0.22745877840756193	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.16910696227810024	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	530	0.15012845897385782	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAATCGCTGTCG	499	0.14134736043010387	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	491	0.13908127048332866	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCGCTGTCGTATGCCGTCTTC	489	0.13851474799663485	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	397	0.1124547136087199	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	391	0.11075514614863852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7053204959337849	0.0
2	0.0	0.0	0.0	4.403862550314279	0.0
3	0.0	0.0	0.0	5.268092603765675	0.0
4	0.0	0.0	0.0	7.871546691367047	0.0
5	0.0	0.0	0.0	15.247102945633669	0.0
6	0.0	0.0	0.0	16.254663188218597	0.0
7	0.0	0.0	0.0	18.0828312527795	0.0
8	0.0	0.0	0.0	20.21748798264175	0.0
9	0.0	0.0	0.0	20.608955020947167	0.0
10	0.0	0.0	0.0	28.73685313754316	0.0
11	0.0	0.0	0.0	30.256832969342636	0.0
12	0.0	0.0	0.0	40.727018307174156	0.0
13	0.0	0.0	0.0	41.75723944922684	0.0
14	0.0	0.0	0.0	42.28013970444522	0.0
15	0.0	0.0	0.0	44.278264515014264	0.0
16	0.0	0.0	0.0	45.308485657066946	0.0
17	0.0	0.0	0.0	46.15685308089091	0.0
18	0.0	0.0	0.0	46.83158136254323	0.0
19	0.0	0.0	0.0	50.124493316450966	0.0
20	0.0	0.0	0.0	50.9618135517844	0.0
21	0.0	0.0	0.0	51.68441298356235	0.0
22	0.0	0.0	0.0	52.50530406678167	0.0
23	0.0	0.0	0.0	53.09363766921319	0.0
24	0.0	0.0	0.0	53.6179542306483	0.0
25	0.0	0.0	0.0	54.06664004010979	0.0
26	0.0	0.0	0.0	54.52637303806181	0.0
27	0.0	0.0	0.0	55.003668233101344	0.0
28	0.0	0.0	0.0	55.38465460540292	0.0
29	0.0	0.0	0.0	55.78660230971218	0.0
30	0.0	0.0	0.0	56.24520226269081	0.0
31	0.0	0.0	0.0	56.68737306355533	0.0
32	0.0	0.0	0.0	57.06411051720671	0.0
33	0.0	0.0	0.0	57.44368058329155	0.0
34	0.0	0.0	0.0	57.80115627239534	0.0
35	0.0	0.0	0.0	58.18894091453725	0.0
36	0.0	0.0	0.0	58.563412278241856	0.0
37	0.0	0.0	0.0	58.94921409168033	0.0
38	0.0	0.0	0.0	59.36079267826338	0.0
39	0.0	0.0	0.0	59.83950417951964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1612123E-6	45.000004	6
CACGCCG	30	2.1612123E-6	45.000004	26
GACTTAT	35	1.2087366E-7	45.000004	23
CTTCTTG	65	0.0	45.000004	1
CGACTGA	35	1.2087366E-7	45.000004	41
AACTACT	30	2.1612123E-6	45.000004	35
CAATCTA	30	2.1612123E-6	45.000004	43
GTTTGCG	40	6.7921064E-9	45.0	1
GAGTGCT	25	3.8852966E-5	45.0	9
AGGTAAC	20	7.026639E-4	45.0	14
GATACCG	20	7.026639E-4	45.0	27
ACCGGTG	90	0.0	45.0	17
CGGCCTC	20	7.026639E-4	45.0	6
GCAACGA	20	7.026639E-4	45.0	9
TTTCGCA	20	7.026639E-4	45.0	14
AAGACGC	20	7.026639E-4	45.0	38
ACGGCTA	25	3.8852966E-5	45.0	30
TTCTTCG	25	3.8852966E-5	45.0	1
TGATTGG	25	3.8852966E-5	45.0	2
GCGATCA	20	7.026639E-4	45.0	33
>>END_MODULE