SRR2933853.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933853.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235104
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	18041	7.673625289233701	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	17353	7.380988838981898	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	11003	4.680056485640397	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGT	7286	3.099054035660814	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	5239	2.2283755274261603	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTA	3890	1.6545869062202259	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	3110	1.3228188376207977	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTC	2937	1.2492343813801552	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	2741	1.1658670205526065	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1795	0.7634919014563767	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	1793	0.7626412141009936	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGT	1286	0.5469919695113652	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1244	0.529127535048319	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATG	1048	0.44576017422077036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	1048	0.44576017422077036	No Hit
TGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCTT	991	0.42151558459235067	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGTAGG	892	0.37940656050088467	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTAT	838	0.3564380019055397	No Hit
GAATCTTTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	646	0.2747720157887573	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	559	0.2377671158295903	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.236065741118824	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	465	0.19778481012658228	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCG	355	0.15099700558050905	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	327	0.13908738260514497	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	311	0.13228188376207975	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	303	0.12887913434054715	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	290	0.12334966653055669	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	272	0.11569348033210833	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	267	0.11356676194365047	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	259	0.11016401252211788	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	240	0.10208248264597795	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	35	1.2064265E-7	45.000004	34
TGGGACG	65	0.0	45.000004	6
CGTTTTT	370	0.0	45.000004	1
TAATCAC	20	7.0218305E-4	45.0	45
CTTGTCG	20	7.0218305E-4	45.0	1
AGCGTTA	25	3.8813116E-5	45.0	25
GGGTGGC	25	3.8813116E-5	45.0	7
AATTTTC	25	3.8813116E-5	45.0	36
TACCATG	20	7.0218305E-4	45.0	25
CGAAAGG	20	7.0218305E-4	45.0	11
TCTCTCG	40	6.7757355E-9	45.0	1
CGAAAAA	20	7.0218305E-4	45.0	22
CTTTTCG	20	7.0218305E-4	45.0	1
TTTGTCG	40	6.7757355E-9	45.0	1
TTTCGCG	55	1.8189894E-12	45.0	1
CATTGGG	20	7.0218305E-4	45.0	3
GATCCAC	25	3.8813116E-5	45.0	35
GCGTTTG	25	3.8813116E-5	45.0	1
TACGGGG	20	7.0218305E-4	45.0	4
GCTTGCG	25	3.8813116E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content