##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933853.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235104
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.033078977814075	25.0	16.0	31.0	16.0	33.0
2	24.73285864978903	28.0	16.0	31.0	16.0	34.0
3	25.960936436640807	30.0	16.0	33.0	16.0	34.0
4	30.602529093507552	32.0	28.0	35.0	19.0	37.0
5	33.04828076085477	35.0	32.0	35.0	28.0	37.0
6	31.029506090921465	35.0	28.0	36.0	17.0	37.0
7	32.96361610181026	35.0	32.0	35.0	28.0	37.0
8	31.18406322308425	35.0	28.0	36.0	17.0	37.0
9	34.04485249081257	35.0	32.0	37.0	28.0	39.0
10	33.53583945828229	35.0	32.0	37.0	28.0	39.0
11	34.26198618483735	35.0	32.0	38.0	27.0	39.0
12	34.28129253436777	35.0	33.0	39.0	27.0	39.0
13	34.55212161426432	35.0	33.0	38.0	27.0	39.0
14	34.259038553150944	36.0	32.0	39.0	25.0	40.0
15	35.01979974819654	37.0	33.0	39.0	27.0	40.0
16	34.851882571117464	36.0	33.0	38.0	27.0	40.0
17	33.9753640941881	36.0	32.0	38.0	27.0	40.0
18	34.398891554375936	36.0	32.0	38.0	27.0	40.0
19	34.14006141962706	36.0	32.0	39.0	27.0	40.0
20	33.532866305975226	35.0	31.0	39.0	25.0	40.0
21	33.51862154620934	35.0	32.0	39.0	25.0	40.0
22	34.96076204573295	37.0	33.0	39.0	27.0	40.0
23	35.07417993738941	37.0	33.0	39.0	27.0	40.0
24	35.553418912481284	37.0	34.0	39.0	29.0	40.0
25	33.77570777187968	36.0	32.0	39.0	24.0	40.0
26	34.502543555192595	36.0	33.0	39.0	27.0	40.0
27	34.523215257928406	37.0	33.0	39.0	27.0	40.0
28	34.641805328705594	37.0	33.0	39.0	25.0	40.0
29	35.21185092554784	37.0	34.0	40.0	27.0	40.0
30	33.435207397577244	36.0	31.0	39.0	23.0	40.0
31	34.51047621478154	37.0	33.0	39.0	25.0	40.0
32	34.47924748196543	37.0	33.0	39.0	26.0	40.0
33	34.73072767796379	37.0	33.0	39.0	26.0	40.0
34	34.77927640533551	37.0	34.0	40.0	25.0	40.0
35	35.26569518170682	38.0	34.0	40.0	27.0	40.0
36	34.618870797604465	37.0	33.0	40.0	25.0	41.0
37	35.06009680822104	38.0	34.0	40.0	27.0	41.0
38	34.6167270654689	37.0	33.0	40.0	25.0	40.0
39	34.620704028855315	37.0	33.0	40.0	25.0	40.0
40	34.39708384374575	37.0	33.0	39.0	25.0	40.0
41	33.84944535184429	36.0	32.0	39.0	24.0	40.0
42	34.61976401932762	37.0	33.0	39.0	26.0	40.0
43	34.70206376752416	37.0	34.0	40.0	26.0	40.0
44	35.234449435143596	38.0	35.0	40.0	29.0	40.0
45	35.545252313869604	38.0	35.0	40.0	30.0	41.0
46	35.3748341159657	38.0	35.0	40.0	29.0	41.0
47	34.58493262556146	37.0	33.0	40.0	24.0	41.0
48	35.0386382196815	37.0	34.0	40.0	27.0	41.0
49	35.54894004355519	38.0	35.0	40.0	29.0	41.0
50	35.01184582142371	38.0	34.0	40.0	26.0	41.0
51	34.401426602694976	37.0	33.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	4.0
11	6.0
12	1.0
13	6.0
14	12.0
15	22.0
16	104.0
17	296.0
18	617.0
19	1052.0
20	1481.0
21	1733.0
22	1733.0
23	1809.0
24	1960.0
25	2436.0
26	3265.0
27	4345.0
28	5742.0
29	7610.0
30	10143.0
31	13643.0
32	18495.0
33	23126.0
34	26648.0
35	30651.0
36	31811.0
37	28648.0
38	15990.0
39	1710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.5639546753777	2.976129712807949	31.253828093099223	12.206087518715123
2	13.187355383149585	40.963998911120186	30.52138628011433	15.327259425615896
3	11.753521845651287	40.773019599836665	34.257179801279435	13.216278753232613
4	13.862801143323805	2.681791887845379	66.70835034708044	16.747056621750374
5	42.11370287192051	3.777902545256567	33.94838029127535	20.160014291547572
6	14.17670477746019	34.24527017830407	36.49363685858174	15.084388185654007
7	50.07741254933986	1.3215428065877228	43.24851980400163	5.352524840070777
8	45.19319109840752	29.438461276711582	14.683289097590855	10.685058527290051
9	42.3416870831632	3.8417040969103033	14.363855995644482	39.45275282428202
10	41.70409691030352	18.652170954130938	21.629151354294272	18.014580781271267
11	35.027051857901185	18.209388185654007	28.423165918061795	18.34039403838301
12	22.409231659180616	13.764121410099362	30.292976725193952	33.533670205526064
13	23.51129712807949	20.607475840479104	42.800207567714715	13.081019463726692
14	17.969069007758268	33.85693140057166	22.202089288144823	25.97191030352525
15	8.997720157887573	21.885633591942288	46.2135905811896	22.90305566898054
16	10.915169456921191	23.769906084115966	24.133149584864572	41.181774874098274
17	12.331989247311828	30.136024908125762	35.44941812984892	22.082567714713488
18	10.61445147679325	23.385395399482782	33.91988226487002	32.08027085885396
19	12.481710221859263	28.24367088607595	27.680090513134616	31.594528378930175
20	16.985249081257656	24.000017013747108	44.80655369538587	14.208180209609363
21	19.829522253981217	37.28009731863345	25.677572478562677	17.212807948822647
22	13.135888798148903	20.617258745066014	36.69992854226215	29.54692391452293
23	16.365948686538722	35.215904450796245	26.867258745066014	21.550888117599023
24	20.753368721927316	22.757588131210017	24.531696610861577	31.95734653600109
25	12.24054035660814	41.369351435960255	21.09704641350211	25.293061793929496
26	13.946593847829044	20.166819790390637	37.289880223220365	28.59670613855996
27	24.709915611814345	26.98210153804274	23.911120185109567	24.396862665033346
28	11.578280250442358	26.604821695930315	40.867020552606505	20.949877501020826
29	25.85621682319314	15.867020552606506	33.44562406424391	24.831138559956443
30	20.69764870014972	31.637062746699335	32.936913025724785	14.72837552742616
31	24.447478562678647	21.258677011024908	26.715411052130122	27.578433374166327
32	36.44684905403566	28.515465496120868	21.227201578875732	13.81048387096774
33	23.342861031713625	17.968643664080577	25.845157887573162	32.84333741663264
34	27.16925275622703	25.341550973186333	30.72342452701783	16.765771743568802
35	34.395841840206884	17.748315639036342	23.244606642166872	24.611235878589902
36	24.176109296311417	20.42415271539404	37.79348713760719	17.606250850687356
37	34.179341908261875	18.26936164420852	32.06410779910168	15.48718864842793
38	23.590836395807813	18.71852456785082	35.177623519804	22.51301551653736
39	30.691949094868654	29.036086157615355	26.072716755138153	14.199247992377842
40	26.858326527834492	18.753828093099223	34.14361303933578	20.2442323397305
41	28.107560909214648	30.219817612631005	20.253589900639717	21.419031577514634
42	30.915679869334422	15.523768204709404	32.99603579692392	20.56451612903226
43	32.925428746427116	15.955917381244046	25.93235334149993	25.18630053082891
44	26.740931672791618	17.349768613039334	32.24062542534368	23.66867428882537
45	19.906934803321082	14.099292228120323	32.296770790798966	33.69700217775963
46	37.254151354294265	23.36370287192051	24.05999047230162	15.322155301483598
47	14.602899142507145	16.673472165509732	50.16035456648973	18.563274125493397
48	23.400707771879677	25.9897747379883	24.218643664080577	26.390873826051447
49	24.09061521709541	13.232867156662584	39.93721927317272	22.73929835306928
50	29.921226350891523	13.800275622703145	29.292143051585683	26.986354974819655
51	21.89158840342997	14.781968830815298	28.18199605281067	35.14444671294406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	80.5
2	121.0
3	532.0
4	943.0
5	691.5
6	440.0
7	578.0
8	716.0
9	818.0
10	920.0
11	952.0
12	984.0
13	924.5
14	865.0
15	814.0
16	763.0
17	700.0
18	637.0
19	652.5
20	668.0
21	764.5
22	861.0
23	898.0
24	935.0
25	984.5
26	1239.5
27	1445.0
28	1551.5
29	1658.0
30	1787.5
31	1917.0
32	2133.0
33	2349.0
34	2778.0
35	3207.0
36	3549.5
37	3892.0
38	4418.0
39	4944.0
40	5541.0
41	6138.0
42	7081.0
43	8024.0
44	10060.5
45	12097.0
46	45235.5
47	78374.0
48	56681.0
49	34988.0
50	30556.5
51	26125.0
52	18449.0
53	10773.0
54	9175.5
55	7578.0
56	6851.5
57	6125.0
58	5443.5
59	4762.0
60	4257.0
61	3752.0
62	3085.0
63	2418.0
64	2036.5
65	1655.0
66	1390.5
67	1126.0
68	904.5
69	683.0
70	603.0
71	523.0
72	437.0
73	351.0
74	260.0
75	101.0
76	33.0
77	39.0
78	45.0
79	33.0
80	21.0
81	11.0
82	1.0
83	2.5
84	4.0
85	2.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	235104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.33132571117463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.31408910283332	20.094511365183067
2	10.021645720931735	5.277664352797061
3	3.500468452169418	2.7651592486729277
4	1.6767356960553097	1.7660269497754184
5	1.0289794204115918	1.3547196134476656
6	0.7947533357025168	1.2556145365455287
7	0.6736019125771331	1.2415781951817069
8	0.5072206248182729	1.0684633183612358
9	0.4409911801763965	1.0450694160881993
>10	4.815365231156915	22.58277187967878
>50	0.1227667754337221	2.287923642302981
>100	0.06946014925855329	3.4061521709541314
>500	0.009692113850030692	1.9059650197359466
>1k	0.016153523083384486	8.886280114332381
>5k	0.003230704616676897	5.327429563086975
>10k+	0.004846056925015346	19.734670613855997
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	18041	7.673625289233701	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	17353	7.380988838981898	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	11003	4.680056485640397	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGT	7286	3.099054035660814	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	5239	2.2283755274261603	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTA	3890	1.6545869062202259	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	3110	1.3228188376207977	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTC	2937	1.2492343813801552	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	2741	1.1658670205526065	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1795	0.7634919014563767	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	1793	0.7626412141009936	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGT	1286	0.5469919695113652	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1244	0.529127535048319	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATG	1048	0.44576017422077036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	1048	0.44576017422077036	No Hit
TGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCTT	991	0.42151558459235067	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGTAGG	892	0.37940656050088467	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTAT	838	0.3564380019055397	No Hit
GAATCTTTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	646	0.2747720157887573	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	559	0.2377671158295903	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.236065741118824	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	465	0.19778481012658228	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCG	355	0.15099700558050905	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	327	0.13908738260514497	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	311	0.13228188376207975	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	303	0.12887913434054715	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	290	0.12334966653055669	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	272	0.11569348033210833	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	267	0.11356676194365047	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	259	0.11016401252211788	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGT	240	0.10208248264597795	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.253436776915748E-4	0.0	0.0	0.9021539403838301	0.0
2	4.253436776915748E-4	0.0	0.0	4.6911154212603785	0.0
3	8.506873553831496E-4	0.0	0.0	5.668980536273309	0.0
4	8.506873553831496E-4	0.0	0.0	8.204879542670477	0.0
5	8.506873553831496E-4	0.0	0.0	14.827480604328297	0.0
6	8.506873553831496E-4	0.0	0.0	16.06565605008847	0.0
7	8.506873553831496E-4	0.0	0.0	18.043078807676604	0.0
8	8.506873553831496E-4	0.0	0.0	20.374812848781815	0.0
9	8.506873553831496E-4	0.0	0.0	20.83290798965564	0.0
10	8.506873553831496E-4	0.0	0.0	29.526507417993738	0.0
11	8.506873553831496E-4	0.0	0.0	31.143664080577107	0.0
12	8.506873553831496E-4	0.0	0.0	40.71815026541446	0.0
13	8.506873553831496E-4	0.0	0.0	41.605842520756774	0.0
14	8.506873553831496E-4	0.0	0.0	42.12093371444127	0.0
15	8.506873553831496E-4	0.0	0.0	44.22595957533687	0.0
16	8.506873553831496E-4	0.0	0.0	45.21318225125902	0.0
17	8.506873553831496E-4	0.0	0.0	46.03919967333606	0.0
18	8.506873553831496E-4	0.0	0.0	46.74612086565945	0.0
19	8.506873553831496E-4	0.0	0.0	50.790288553150944	0.0
20	8.506873553831496E-4	0.0	0.0	51.72391792568395	0.0
21	8.506873553831496E-4	0.0	0.0	52.299833265278345	0.0
22	8.506873553831496E-4	0.0	0.0	53.0441847012386	0.0
23	8.506873553831496E-4	0.0	0.0	53.5945794201715	0.0
24	8.506873553831496E-4	0.0	0.0	54.028429971416905	0.0
25	8.506873553831496E-4	0.0	0.0	54.38146522390091	0.0
26	8.506873553831496E-4	0.0	0.0	54.82254661766707	0.0
27	8.506873553831496E-4	0.0	0.0	55.25937457465632	0.0
28	8.506873553831496E-4	0.0	0.0	55.62134204437185	0.0
29	8.506873553831496E-4	0.0	0.0	55.98671226350891	0.0
30	8.506873553831496E-4	0.0	0.0	56.39206478834899	0.0
31	8.506873553831496E-4	0.0	0.0	56.785082346536	0.0
32	8.506873553831496E-4	0.0	0.0	57.139393630053085	0.0
33	8.506873553831496E-4	0.0	0.0	57.46988566761944	0.0
34	8.506873553831496E-4	0.0	0.0	57.77740914659044	0.0
35	8.506873553831496E-4	0.0	0.0	58.11938546345447	0.0
36	8.506873553831496E-4	0.0	0.0	58.43541581597931	0.0
37	8.506873553831496E-4	0.0	0.0	58.76803457193412	0.0
38	8.506873553831496E-4	0.0	0.0	59.09044507962434	0.0
39	8.506873553831496E-4	0.0	0.0	59.40137130801688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	35	1.2064265E-7	45.000004	34
TGGGACG	65	0.0	45.000004	6
CGTTTTT	370	0.0	45.000004	1
TAATCAC	20	7.0218305E-4	45.0	45
CTTGTCG	20	7.0218305E-4	45.0	1
AGCGTTA	25	3.8813116E-5	45.0	25
GGGTGGC	25	3.8813116E-5	45.0	7
AATTTTC	25	3.8813116E-5	45.0	36
TACCATG	20	7.0218305E-4	45.0	25
CGAAAGG	20	7.0218305E-4	45.0	11
TCTCTCG	40	6.7757355E-9	45.0	1
CGAAAAA	20	7.0218305E-4	45.0	22
CTTTTCG	20	7.0218305E-4	45.0	1
TTTGTCG	40	6.7757355E-9	45.0	1
TTTCGCG	55	1.8189894E-12	45.0	1
CATTGGG	20	7.0218305E-4	45.0	3
GATCCAC	25	3.8813116E-5	45.0	35
GCGTTTG	25	3.8813116E-5	45.0	1
TACGGGG	20	7.0218305E-4	45.0	4
GCTTGCG	25	3.8813116E-5	45.0	1
>>END_MODULE