FastQCFastQC Report
Sat 14 Jan 2017
SRR2933852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664550
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGC158422.3838687833872543No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTGTGGCT135952.0457452411406214No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCG135732.042434730268603No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGC107281.6143254834098262No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTC80711.214506056730118No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTA53150.7997893311263261No Hit
GCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGC41780.6286961101497254Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCT40720.6127454668572718No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTC36870.5548115265969453No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATTCCG31220.4697915882928298No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCT27640.41592054773907156No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTT27390.4121586035663231No Hit
CCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGC24740.37228199533518924Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGCCG24620.37047626213227No Hit
CTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGCT23670.35618087427582573Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTAATCCG23090.34745316379504926No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCAATGC20990.3158528327439621No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.30983372206756454No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGT17060.25671507034835606No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTTGTGGC16010.24091490482281244No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGAATGC15880.23895869385298324No Hit
TCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGC15050.2264690391994583Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGCCGTCTTT14790.2225566172597999No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTAATCCG14590.21954706192160106No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATTCCG14130.21262508464374388No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTAAGCCG13310.2002859077571289No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGCCGTCTTC12950.19486870814837107No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGGAATCCG11250.16928748777368144No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCCATGC11010.1656760213678429No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCT10340.155594010984877No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCGAATCCG9950.14972537807538935No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGCATGC9490.14280340079753218No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTAT9450.1422014897298924No Hit
CGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTG9200.13843954555714394No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCCGATGCCGGCT8710.13106613497855693No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGGATGCCG8600.1294108795425476No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTT7910.11902791362576179No Hit
GCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTTC7770.11692122488902265No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATTC7320.1101497253780754No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGGCTTC6950.10458204800240765No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGCA6860.10322774810021819No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGAA6830.10277631479948839No Hit
GCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGGCGTCTTCTGC6800.10232488149875855No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGGA6780.10202392596493869No Hit
GAACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTTT6770.10187344819802874No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCC6740.10142201489729892No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATG6690.10066962606274923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGA207.0311356E-445.00000415
TAGAGCG207.0311356E-445.00000433
ACTTCCG207.0311356E-445.0000041
TACGGGA207.0311356E-445.0000044
TCGGCGA453.8380676E-1045.00000430
TGGTTCG207.0311356E-445.0000041
AAACGTA207.0311356E-445.00000424
GTCGTGA207.0311356E-445.0000049
GTTCCGT207.0311356E-445.00000442
TTAACCG207.0311356E-445.00000416
GACATGT207.0311356E-445.00000412
CGGTAGT800.045.00000412
CACCGGT1700.045.00000416
TATCCGG207.0311356E-445.0000042
TCTTGCG1000.045.01
GGTTCGT253.889024E-545.09
CGTCCGG351.2109012E-745.02
TCGTTAG302.1641135E-644.9999961
GGTACCC302.1641135E-644.9999968
TTACCGG302.1641135E-644.9999962
Produced by FastQC (version 0.11.5)