##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933852.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 664550 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.06114062147318 28.0 16.0 31.0 16.0 34.0 2 25.922011887743587 30.0 16.0 33.0 16.0 34.0 3 26.95098186742909 31.0 16.0 33.0 16.0 34.0 4 31.265742231585282 33.0 28.0 35.0 19.0 37.0 5 33.358099465803924 35.0 32.0 35.0 28.0 37.0 6 31.720039124219397 35.0 30.0 37.0 17.0 37.0 7 33.26542171394176 35.0 32.0 35.0 28.0 37.0 8 32.127955759536526 35.0 32.0 37.0 17.0 37.0 9 34.33264765630878 35.0 32.0 39.0 28.0 39.0 10 33.57714242720638 35.0 32.0 37.0 27.0 39.0 11 34.40797080731322 35.0 32.0 39.0 27.0 39.0 12 34.45954555714393 37.0 33.0 39.0 27.0 39.0 13 34.767907606651114 37.0 34.0 39.0 30.0 39.0 14 34.695023700248285 37.0 33.0 39.0 27.0 40.0 15 35.21382740200136 37.0 33.0 39.0 27.0 40.0 16 34.68072680761418 36.0 32.0 39.0 27.0 40.0 17 34.17386803099842 36.0 32.0 38.0 27.0 40.0 18 34.5449281468663 36.0 32.0 39.0 27.0 40.0 19 34.3024542923783 36.0 32.0 39.0 27.0 40.0 20 34.22781431043563 36.0 32.0 39.0 26.0 40.0 21 34.0528854111805 36.0 32.0 39.0 25.0 40.0 22 35.318820254307425 37.0 34.0 39.0 29.0 40.0 23 35.7158362801896 37.0 34.0 39.0 30.0 40.0 24 35.987228951922354 38.0 34.0 39.0 30.0 40.0 25 34.03974268301858 37.0 32.0 39.0 25.0 40.0 26 34.74005266721842 37.0 33.0 39.0 27.0 40.0 27 35.194310435633135 37.0 34.0 39.0 29.0 40.0 28 35.455250921676324 37.0 34.0 39.0 29.0 40.0 29 35.94417425325408 38.0 34.0 40.0 30.0 40.0 30 34.198302610789256 37.0 33.0 39.0 24.0 40.0 31 35.027487773681436 37.0 33.0 39.0 27.0 40.0 32 35.11332029192687 37.0 34.0 39.0 29.0 40.0 33 35.533672409901435 38.0 34.0 39.0 30.0 40.0 34 35.540744864946205 38.0 35.0 40.0 29.0 40.0 35 35.5567225942367 38.0 35.0 40.0 28.0 41.0 36 34.51260702731172 38.0 33.0 40.0 23.0 41.0 37 34.72117673613724 38.0 34.0 40.0 22.0 41.0 38 33.85074411255737 37.0 33.0 40.0 18.0 41.0 39 32.42088480926943 37.0 30.0 40.0 15.0 40.0 40 31.5409886389286 35.0 28.0 39.0 10.0 40.0 41 30.907714995109473 35.0 25.0 39.0 9.0 40.0 42 31.588518546384773 36.0 28.0 39.0 12.0 40.0 43 30.994856669927017 36.0 25.0 39.0 9.0 40.0 44 30.372372281995336 35.0 23.0 40.0 9.0 40.0 45 30.42930404032804 35.0 23.0 40.0 9.0 41.0 46 27.7078985779851 33.0 15.0 39.0 7.0 40.0 47 27.163950041381387 33.0 14.0 39.0 7.0 41.0 48 27.352644646753443 33.0 14.0 39.0 8.0 40.0 49 27.35037544202844 32.0 14.0 39.0 8.0 41.0 50 26.94112105936348 32.0 13.0 39.0 8.0 41.0 51 25.25358814235197 28.0 12.0 38.0 8.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 4.0 11 10.0 12 10.0 13 16.0 14 22.0 15 46.0 16 123.0 17 336.0 18 837.0 19 1618.0 20 2440.0 21 3276.0 22 4263.0 23 5521.0 24 8113.0 25 12459.0 26 18971.0 27 25975.0 28 34534.0 29 43605.0 30 52869.0 31 60693.0 32 64152.0 33 59902.0 34 51569.0 35 50948.0 36 57384.0 37 62201.0 38 38599.0 39 4047.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.82965916785795 3.224437589346174 38.813633285682045 12.132269957113836 2 15.46189150553006 31.91798961703408 35.5533819878113 17.066736889624558 3 13.38469641110526 31.870589120457453 39.317583327063424 15.427131141373863 4 14.64840869761493 2.881047325257693 63.828455345722666 18.64208863140471 5 35.628921826800095 3.2802648408697612 39.9787826348657 21.112030697464448 6 15.119253630276127 26.513881573997445 42.223309006094354 16.14355578963208 7 56.59814912346701 1.16033406064254 36.341885486419386 5.899631329471071 8 52.457602889173124 21.421713941765105 15.277556241065382 10.843126927996389 9 50.03009555338199 2.845534572266948 15.793243548265743 31.331126326085325 10 39.27560003009555 15.642765781355806 25.526596945301332 19.555037243247313 11 29.68610337822587 20.731923858249942 28.629298021217366 20.952674742306822 12 19.720111353547512 15.745993529456022 33.66037168008427 30.873523436912198 13 21.466255360770443 20.782634865698594 42.97329019637349 14.777819577157475 14 19.665638401926117 28.926190655330675 26.723798058836806 24.684372883906402 15 11.53201414491009 23.67556993454217 43.78797682642389 21.004439094123846 16 12.437890301707922 25.020088781882478 25.290798284553457 37.251222631856145 17 13.16138740501091 29.098638176209462 34.978406440448424 22.7615679783312 18 12.400722293281168 24.21744037318486 35.198705891204575 28.183131442329394 19 13.307501316680462 28.993905650440148 29.01346776013844 28.68512527274095 20 15.593409073809344 26.307426077797007 42.49446994206606 15.604694906327591 21 18.654427808291324 34.792716876081556 28.146113911669552 18.406741403957565 22 13.58182228575728 22.07674366112407 34.906779023399295 29.43465502971936 23 15.77834624934166 32.36852005116244 28.899556090587613 22.953577608908287 24 20.62026935520277 22.432774057632983 26.23128432774058 30.71567225942367 25 12.327289143029118 37.211195545858104 23.43856745165902 27.022947859453765 26 13.914227672861335 21.06688736739147 36.81288089684749 28.20600406289971 27 19.5261455120006 26.086524715973212 29.7062673989918 24.681062373034386 28 11.122263185614326 23.89917989617034 41.673613723572345 23.30494319464299 29 21.87495297569784 16.426905424723497 35.85659468813483 25.841546911443835 30 15.929124971785418 26.814987585584234 40.94484989842751 16.311037544202843 31 25.203521179745692 18.68873673914679 30.535098939131743 25.57264314197577 32 29.412685275750505 27.002783838687833 26.93401549920999 16.650515386351668 33 18.10563539237078 16.56730118125047 30.970581596569108 34.356481829809645 34 30.02979459784817 18.244375893461743 32.5555639154315 19.170265593258595 35 22.19050485290798 17.03724324731021 32.5874652020164 28.184786697765407 36 16.5465352494169 18.155594010984878 39.814159957866224 25.483710781732 37 23.23903393273644 17.020239259649387 41.24956737642013 18.491159431194042 38 20.350763674667068 19.43239786321571 35.76435181701903 24.452486645098187 39 31.320743360168535 19.04431570235498 29.099841998344743 20.535098939131743 40 25.396885110224964 16.685576706041683 35.99533518922579 21.92220299450756 41 26.758407945226093 19.8304115566925 24.17771424272064 29.233466255360767 42 27.18952674742307 15.720713264615153 32.952674742306826 24.137085245654955 43 21.570837408772853 16.34790459709578 28.93055451057106 33.15070348356031 44 20.438341735008652 15.734256263637047 35.040704235949136 28.78669776540516 45 20.81935144082462 13.530960800541719 32.035663230757656 33.614024527876005 46 28.223910917161987 19.709577909863818 28.385223083289446 23.68128808968475 47 14.757354600857722 18.857723271386654 44.46873824392446 21.916183883831163 48 21.238281543901888 19.87299676472801 31.58106989692273 27.30765179444737 49 22.933112632608534 12.726055225340454 41.37295914528629 22.96787299676473 50 23.540440899857046 13.358513279662928 32.618915055300576 30.482130765179445 51 20.255812203746896 12.931457377172523 31.806034158453087 35.00669626062749 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 156.0 1 223.0 2 290.0 3 1906.5 4 3523.0 5 2607.5 6 1692.0 7 1703.5 8 1715.0 9 1962.5 10 2210.0 11 2293.0 12 2376.0 13 2392.0 14 2408.0 15 2207.0 16 2006.0 17 2049.5 18 2093.0 19 2097.0 20 2101.0 21 2185.5 22 2270.0 23 2470.5 24 2671.0 25 2808.5 26 3301.5 27 3657.0 28 4143.0 29 4629.0 30 5150.5 31 5672.0 32 6677.5 33 7683.0 34 8838.5 35 9994.0 36 11004.0 37 12014.0 38 14112.0 39 16210.0 40 19060.0 41 21910.0 42 26676.5 43 31443.0 44 47990.0 45 64537.0 46 108899.0 47 153261.0 48 134805.0 49 116349.0 50 97285.5 51 78222.0 52 60762.0 53 43302.0 54 33631.5 55 23961.0 56 19187.0 57 14413.0 58 11995.0 59 9577.0 60 8124.5 61 6672.0 62 5824.0 63 4976.0 64 3900.0 65 2824.0 66 2412.0 67 2000.0 68 1531.5 69 1063.0 70 906.0 71 749.0 72 608.5 73 468.0 74 356.0 75 212.5 76 181.0 77 114.5 78 48.0 79 33.5 80 19.0 81 14.5 82 10.0 83 7.5 84 5.0 85 2.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 664550.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.695081601741634 #Duplication Level Percentage of deduplicated Percentage of total 1 77.780249038207 29.31932834498999 2 10.627078993031892 8.01177219660983 3 3.6710067588563793 4.151366980069089 4 1.779255298449902 2.6827669466160087 5 1.0554521757326718 1.9892677945489408 6 0.7509984798325282 1.6985369388042644 7 0.5844086877189879 1.5420533220633794 8 0.44293701498011795 1.3357237539285922 9 0.396662640441522 1.3457007539824926 >10 2.704701216631755 16.9689455993348 >50 0.09795786419630968 2.597740407945891 >100 0.08338561991004792 6.817294781780893 >500 0.013762675130784607 3.520415076551596 >1k 0.009714829504083254 7.777047331690287 >5k 8.095691253402709E-4 2.0424877000138553 >10k+ 0.0016191382506805419 8.199552071070114 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGC 15842 2.3838687833872543 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTGTGGCT 13595 2.0457452411406214 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCG 13573 2.042434730268603 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGC 10728 1.6143254834098262 No Hit GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTC 8071 1.214506056730118 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTA 5315 0.7997893311263261 No Hit GCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGC 4178 0.6286961101497254 Illumina Single End Adapter 1 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCT 4072 0.6127454668572718 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTC 3687 0.5548115265969453 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATTCCG 3122 0.4697915882928298 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCT 2764 0.41592054773907156 No Hit GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTT 2739 0.4121586035663231 No Hit CCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGC 2474 0.37228199533518924 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGCCG 2462 0.37047626213227 No Hit CTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGCT 2367 0.35618087427582573 Illumina Single End Adapter 1 (95% over 22bp) GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTAATCCG 2309 0.34745316379504926 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCAATGC 2099 0.3158528327439621 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.30983372206756454 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGT 1706 0.25671507034835606 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTTGTGGC 1601 0.24091490482281244 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGAATGC 1588 0.23895869385298324 No Hit TCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTGC 1505 0.2264690391994583 Illumina Single End Adapter 1 (95% over 21bp) GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGCCGTCTTT 1479 0.2225566172597999 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTAATCCG 1459 0.21954706192160106 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATTCCG 1413 0.21262508464374388 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTAAGCCG 1331 0.2002859077571289 No Hit GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATGCCGTCTTC 1295 0.19486870814837107 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGGAATCCG 1125 0.16928748777368144 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCCATGC 1101 0.1656760213678429 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCT 1034 0.155594010984877 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCGAATCCG 995 0.14972537807538935 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGCATGC 949 0.14280340079753218 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTAT 945 0.1422014897298924 No Hit CGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTG 920 0.13843954555714394 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCCGATGCCGGCT 871 0.13106613497855693 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGGATGCCG 860 0.1294108795425476 No Hit GAATGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTT 791 0.11902791362576179 No Hit GCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTCTTC 777 0.11692122488902265 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTGTGGCTCCTATTC 732 0.1101497253780754 No Hit GAATCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGGCTTC 695 0.10458204800240765 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGCA 686 0.10322774810021819 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGAA 683 0.10277631479948839 No Hit GCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGGCGTCTTCTGC 680 0.10232488149875855 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGGA 678 0.10202392596493869 No Hit GAACTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCCGTCTTTT 677 0.10187344819802874 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATGCC 674 0.10142201489729892 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTTGTGGCTCGTATG 669 0.10066962606274923 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.9377774433827402 0.0 2 0.0 0.0 0.0 4.796027386953577 0.0 3 0.0 0.0 0.0 5.936498382364006 0.0 4 0.0 0.0 0.0 8.664058385373561 0.0 5 0.0 0.0 0.0 15.253630276126703 0.0 6 0.0 0.0 0.0 16.577232713866525 0.0 7 0.0 0.0 0.0 18.672936573621246 0.0 8 0.0 0.0 0.0 21.260251297870738 0.0 9 0.0 0.0 0.0 21.85042509969152 0.0 10 0.0 0.0 0.0 28.907681890000752 0.0 11 0.0 0.0 0.0 31.48401173726582 0.0 12 0.0 0.0 0.0 40.06500639530509 0.0 13 0.0 0.0 0.0 41.143631028515536 0.0 14 0.0 0.0 0.0 41.667143179595215 0.0 15 0.0 0.0 0.0 43.48717177037093 0.0 16 0.0 0.0 0.0 44.72500188097209 0.0 17 0.0 0.0 0.0 46.106538258972236 0.0 18 0.0 0.0 0.0 47.31051087201866 0.0 19 0.0 0.0 0.0 50.60477014521104 0.0 20 1.5047776690993905E-4 0.0 0.0 51.719509442479875 0.0 21 1.5047776690993905E-4 0.0 0.0 53.00308479422166 0.0 22 1.5047776690993905E-4 0.0 0.0 54.38973741629674 0.0 23 1.5047776690993905E-4 0.0 0.0 55.77488526070273 0.0 24 1.5047776690993905E-4 0.0 0.0 56.748175457076215 0.0 25 1.5047776690993905E-4 0.0 0.0 57.56301256489354 0.0 26 1.5047776690993905E-4 0.0 0.0 58.30366413362426 0.0 27 1.5047776690993905E-4 0.0 0.0 59.025505981491236 0.0 28 1.5047776690993905E-4 0.0 0.0 59.79128733729591 0.0 29 1.5047776690993905E-4 0.0 0.0 60.563689714844635 0.0 30 1.5047776690993905E-4 0.0 0.0 61.321947182303816 0.0 31 1.5047776690993905E-4 0.0 0.0 62.05176435181702 0.0 32 1.5047776690993905E-4 0.0 0.0 62.75163644571514 0.0 33 1.5047776690993905E-4 0.0 0.0 63.425626363704765 0.0 34 1.5047776690993905E-4 0.0 0.0 64.05116244074938 0.0 35 1.5047776690993905E-4 0.0 0.0 64.65894214129862 0.0 36 1.5047776690993905E-4 0.0 0.0 65.2611541644722 0.0 37 1.5047776690993905E-4 0.0 0.0 65.84816793318787 0.0 38 1.5047776690993905E-4 0.0 0.0 66.46061244451133 0.0 39 1.5047776690993905E-4 0.0 0.0 67.0340832142051 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTGA 20 7.0311356E-4 45.000004 15 TAGAGCG 20 7.0311356E-4 45.000004 33 ACTTCCG 20 7.0311356E-4 45.000004 1 TACGGGA 20 7.0311356E-4 45.000004 4 TCGGCGA 45 3.8380676E-10 45.000004 30 TGGTTCG 20 7.0311356E-4 45.000004 1 AAACGTA 20 7.0311356E-4 45.000004 24 GTCGTGA 20 7.0311356E-4 45.000004 9 GTTCCGT 20 7.0311356E-4 45.000004 42 TTAACCG 20 7.0311356E-4 45.000004 16 GACATGT 20 7.0311356E-4 45.000004 12 CGGTAGT 80 0.0 45.000004 12 CACCGGT 170 0.0 45.000004 16 TATCCGG 20 7.0311356E-4 45.000004 2 TCTTGCG 100 0.0 45.0 1 GGTTCGT 25 3.889024E-5 45.0 9 CGTCCGG 35 1.2109012E-7 45.0 2 TCGTTAG 30 2.1641135E-6 44.999996 1 GGTACCC 30 2.1641135E-6 44.999996 8 TTACCGG 30 2.1641135E-6 44.999996 2 >>END_MODULE