FastQCFastQC Report
Sat 14 Jan 2017
SRR2933850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443930
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC357678.05690086274863No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG292566.590228189128916No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC240025.406708264816525No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC118482.6688892392944834No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCTATT118062.659428288243642No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTA73691.6599463879440453No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT71571.612191111211227No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC58021.3069628094519405No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT53591.207172301939495No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT37170.8372941679994593No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC32740.7375036604870138No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC30830.6944788592796162No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGT26700.6014461739463429No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATG21840.4919694546437502No Hit
TGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCTT17050.3840695605162976Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTAT16080.3622192688036402No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTCCTAT14060.3167165994638794No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT12920.29103687518302435No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.24193003401437163No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG10670.24035320883923142No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC8770.1975536683711396No Hit
TCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG7490.16872029374000405No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT7170.1615119500822202No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCTCCTATTCG7160.1612866893429144No Hit
TTTCCCGGGTTCATTAGGAAGTACTGCCTAGCCCTGCTATTCAGCATTATA7000.15768251751402249No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC6620.14912260942040412No Hit
TGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG6550.1475457842452639No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC5550.12501971031468925No Hit
GCACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT4790.10789989412745252No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG4730.10654832969161805No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC4510.10159259342689163No Hit
GACTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC4460.1004662897303629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTCG551.8189894E-1245.00000412
ATGGGCG253.8869235E-545.05
ACCGGTC253.8869235E-545.017
CCGGGAT351.2096825E-745.05
GACGTGT207.0286036E-445.026
TATGGGA351.2096825E-745.04
TAGCGGT207.0286036E-445.03
CCTCGCT207.0286036E-445.026
CGTTAGG207.0286036E-445.02
AGCGGTA207.0286036E-445.04
ACGATAC207.0286036E-445.03
GTTAAAT207.0286036E-445.010
AACGCTT207.0286036E-445.019
ACGTGTA207.0286036E-445.027
GCCCTCG207.0286036E-445.024
GTCGTTG207.0286036E-445.01
TCGTCCG207.0286036E-445.01
GGTATAT207.0286036E-445.08
AACGATA207.0286036E-445.02
CGGGATC351.2096825E-745.06
Produced by FastQC (version 0.11.5)