##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933850.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443930
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.971491000833463	25.0	16.0	31.0	16.0	33.0
2	24.71251773928322	28.0	16.0	31.0	16.0	34.0
3	25.86178226296939	30.0	16.0	33.0	16.0	34.0
4	30.514430202959925	32.0	28.0	35.0	19.0	37.0
5	33.0406865947334	35.0	32.0	35.0	28.0	37.0
6	30.983801500236524	35.0	28.0	35.0	17.0	37.0
7	33.025573851733384	35.0	32.0	35.0	28.0	37.0
8	31.52756290406145	35.0	28.0	36.0	17.0	37.0
9	34.05320883923141	35.0	32.0	37.0	28.0	39.0
10	33.399087694005814	34.0	32.0	37.0	27.0	39.0
11	34.23335886288379	35.0	32.0	38.0	27.0	39.0
12	34.35040434302705	35.0	33.0	39.0	27.0	39.0
13	34.557434730700784	35.0	33.0	37.0	29.0	39.0
14	34.38275629040614	36.0	32.0	38.0	27.0	40.0
15	34.97887054265312	37.0	33.0	39.0	27.0	40.0
16	34.47528213907598	36.0	32.0	38.0	27.0	40.0
17	33.92519766629874	36.0	32.0	38.0	27.0	40.0
18	34.31261685400852	36.0	32.0	38.0	27.0	40.0
19	34.0437704142545	36.0	32.0	39.0	27.0	40.0
20	33.6404613339941	35.0	31.0	39.0	25.0	40.0
21	33.77718784493051	36.0	32.0	39.0	25.0	40.0
22	35.0949699276913	37.0	33.0	39.0	28.0	40.0
23	35.280113981934086	37.0	33.0	39.0	29.0	40.0
24	35.58499087694006	37.0	34.0	39.0	29.0	40.0
25	33.75183700132904	36.0	32.0	39.0	24.0	40.0
26	34.73298943527133	37.0	33.0	39.0	27.0	40.0
27	34.87075214560854	37.0	33.0	39.0	27.0	40.0
28	34.9273534115739	37.0	33.0	39.0	27.0	40.0
29	35.32672042889645	37.0	34.0	40.0	27.0	40.0
30	33.87688824814723	37.0	32.0	39.0	24.0	40.0
31	34.44627981889037	37.0	32.0	39.0	25.0	40.0
32	34.81996936453945	37.0	33.0	39.0	27.0	40.0
33	35.18788097222535	37.0	34.0	39.0	27.0	40.0
34	35.28140472597031	38.0	34.0	40.0	28.0	40.0
35	35.346833960309056	38.0	34.0	40.0	27.0	40.0
36	35.226738449755594	38.0	34.0	40.0	27.0	41.0
37	35.86727637240105	38.0	35.0	40.0	30.0	41.0
38	35.254999662108894	37.0	34.0	40.0	29.0	40.0
39	35.25066114026986	37.0	34.0	40.0	29.0	40.0
40	34.78165476539094	37.0	33.0	39.0	27.0	40.0
41	34.190187642195845	36.0	33.0	39.0	25.0	40.0
42	35.00265131890163	37.0	34.0	40.0	27.0	40.0
43	35.152145608541886	37.0	34.0	40.0	28.0	40.0
44	35.916284098844415	38.0	35.0	40.0	31.0	40.0
45	36.052976820669926	38.0	35.0	40.0	31.0	41.0
46	35.76760074786566	38.0	35.0	40.0	30.0	41.0
47	35.03895433964814	37.0	34.0	40.0	26.0	41.0
48	35.492428986551936	37.0	34.0	40.0	29.0	41.0
49	35.76189939855383	38.0	35.0	40.0	30.0	41.0
50	35.312700200482055	38.0	34.0	40.0	28.0	41.0
51	35.11289392471786	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	5.0
12	6.0
13	6.0
14	17.0
15	34.0
16	77.0
17	228.0
18	631.0
19	1345.0
20	1991.0
21	2433.0
22	2589.0
23	2559.0
24	3052.0
25	4013.0
26	5536.0
27	7534.0
28	10073.0
29	13682.0
30	18767.0
31	25478.0
32	34695.0
33	44728.0
34	54131.0
35	62434.0
36	63788.0
37	54583.0
38	27226.0
39	2279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.22708535129412	3.100939337282905	33.44671457211722	10.225260739305748
2	13.458878652039735	39.29786227558399	31.111887009213163	16.13137206316311
3	11.450904421868312	39.36386367220057	35.245421575473614	13.939810330457506
4	13.803978104656139	2.7141666478949387	66.02302164755704	17.458833599891875
5	40.29666839366567	2.9184781384452503	35.34048160746063	21.444371860428447
6	13.656432320410875	32.185930214222964	38.02468857702791	16.13294888833825
7	50.767688599553985	1.033946793413376	42.38370914333341	5.814655463699232
8	44.530218728177864	27.222084562881538	16.13137206316311	12.116324645777487
9	42.505575203297816	2.9396526479399903	16.24220034690154	38.312571801860656
10	41.229022593652154	13.76951321154236	25.35670038069065	19.64476381411484
11	32.44362849998873	18.4986371725272	29.027098866938484	20.030635460545582
12	19.61119996395828	14.57707296195346	32.40037843804203	33.41134863604622
13	21.713107922420203	20.896988263915482	44.57910030860721	12.810803505057104
14	19.252359606244227	31.456986461829565	23.964138490302524	25.32651544162368
15	8.653616561169555	22.879057509066744	46.72988984749848	21.737436082265223
16	9.536413398508774	24.658842610321447	23.89498344333566	41.90976054783412
17	11.930935057328858	29.393372829049625	35.68468001711982	22.991012096501702
18	10.760029734417587	23.72513684589913	34.37050886401009	31.14432455567319
19	11.40810488140022	28.583560471245466	28.666681684049287	31.341652963305027
20	14.344829139729237	25.887865203973597	45.436217421665575	14.331088234631586
21	18.479490009686213	36.71772576757597	27.01506994345955	17.787714279278262
22	11.883855562813956	21.725497263082016	36.133624670556166	30.25702250354786
23	15.031198612393846	34.870587705268846	27.801680445115224	22.296533237222086
24	21.407429099182306	22.79188160295542	23.256143986664565	32.54454531119771
25	11.393237672606041	40.72488905908589	20.1216407992251	27.76023246908296
26	17.519653999504424	20.054287838172684	31.52524046583921	30.900817696483678
27	20.565404455657426	27.545784245263892	21.724596220124795	30.164215078953887
28	11.82010677359043	23.790687721037102	38.07965219741851	26.30955330795396
29	25.45153515193837	14.639244926001846	24.955736264726422	34.953483657333365
30	13.367873313360215	28.619827450273693	29.76415200594688	28.248147230419214
31	21.159867546685287	18.328340053612056	29.06066271709504	31.451129682607622
32	21.048814002207557	32.52292028022436	18.344108305363456	28.084157412204625
33	12.600860496024147	15.995314576622441	25.80789764152006	45.595927285833355
34	27.935935845741444	20.265582411641475	30.450521478611492	21.347960264005586
35	13.635257810916135	23.51789696573784	23.416980154528865	39.42986506881716
36	23.76568377897416	18.268645957696034	35.30511567138963	22.660554591940173
37	11.280382042213862	17.537674858648884	35.387561101975535	35.794381997161715
38	15.542315229878584	21.231725722523823	29.59655801590341	33.62940103169419
39	19.0318293424639	18.95659225553578	34.33491766719978	27.676660734800528
40	15.064987723289708	24.552970062847745	27.05561687653459	33.32642533732796
41	15.594125199918906	22.75764197058095	38.35694816750389	23.29128466199626
42	16.113351204018652	24.437636564323203	34.73768386907846	24.711328362579685
43	24.534723942963982	16.071002185029172	30.84382672943933	28.55044714256752
44	19.181177212623613	15.069943459554432	33.898137093685946	31.850742234136014
45	16.347847633635933	14.49935800689298	31.051066609600614	38.10172774987047
46	29.938278557430227	21.44932759669317	26.59203027504336	22.02036357083324
47	10.623746987137611	15.27830964341225	48.846214493275966	25.251728876174173
48	19.306422183677608	21.92124884553871	29.141756583245105	29.630572387538578
49	16.093753519699053	15.496587299799518	43.75261865609443	24.657040524407
50	23.987340346451017	12.508503592908793	33.690446692046045	29.81370936859415
51	18.33825152614151	13.551460815894398	27.844479985583316	40.26580767238078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	159.0
2	255.0
3	976.0
4	1697.0
5	1167.0
6	637.0
7	812.0
8	987.0
9	1235.5
10	1484.0
11	1558.0
12	1632.0
13	1615.0
14	1598.0
15	1519.0
16	1440.0
17	1410.0
18	1380.0
19	1309.5
20	1239.0
21	1353.0
22	1467.0
23	1464.0
24	1461.0
25	1658.5
26	2120.5
27	2385.0
28	2395.5
29	2406.0
30	2682.5
31	2959.0
32	3517.0
33	4075.0
34	4864.5
35	5654.0
36	6369.0
37	7084.0
38	7453.0
39	7822.0
40	9009.0
41	10196.0
42	12105.0
43	14014.0
44	18106.0
45	22198.0
46	88262.5
47	154327.0
48	113398.5
49	72470.0
50	63592.5
51	54715.0
52	39196.0
53	23677.0
54	19013.5
55	14350.0
56	11825.0
57	9300.0
58	7896.5
59	6493.0
60	5691.0
61	4889.0
62	3970.0
63	3051.0
64	2468.0
65	1885.0
66	1486.0
67	1087.0
68	842.5
69	598.0
70	506.5
71	415.0
72	379.0
73	343.0
74	284.5
75	158.5
76	91.0
77	56.0
78	21.0
79	11.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.077999055328743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6238851307419	20.477434901305184
2	11.194218168316187	6.0623405797361904
3	4.068329315351794	3.304866520735863
4	2.017785462739417	2.18550371415656
5	1.1902636985520332	1.611497965249202
6	0.7765238293603511	1.2616026910715905
7	0.5982521703912723	1.1339630193292267
8	0.46269776728820367	1.0023143764426157
9	0.3999437572734139	0.9746709013470717
>10	3.43047739256378	16.589103849230018
>50	0.12132175907091546	2.2885037912661734
>100	0.09287389832325252	5.254881391409526
>500	0.00669361429356775	1.275771195784558
>1k	0.009203719653655655	5.229053311793217
>5k	0.003346807146783875	5.819700711439877
>10k+	0.004183508933479843	25.52879107970312
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	35767	8.05690086274863	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	29256	6.590228189128916	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	24002	5.406708264816525	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	11848	2.6688892392944834	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCTATT	11806	2.659428288243642	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTA	7369	1.6599463879440453	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	7157	1.612191111211227	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC	5802	1.3069628094519405	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT	5359	1.207172301939495	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	3717	0.8372941679994593	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3274	0.7375036604870138	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3083	0.6944788592796162	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGT	2670	0.6014461739463429	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATG	2184	0.4919694546437502	No Hit
TGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCTT	1705	0.3840695605162976	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTAT	1608	0.3622192688036402	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTCCTAT	1406	0.3167165994638794	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT	1292	0.29103687518302435	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1074	0.24193003401437163	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1067	0.24035320883923142	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	877	0.1975536683711396	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	749	0.16872029374000405	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	717	0.1615119500822202	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCTCCTATTCG	716	0.1612866893429144	No Hit
TTTCCCGGGTTCATTAGGAAGTACTGCCTAGCCCTGCTATTCAGCATTATA	700	0.15768251751402249	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	662	0.14912260942040412	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	655	0.1475457842452639	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC	555	0.12501971031468925	No Hit
GCACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	479	0.10789989412745252	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	473	0.10654832969161805	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	451	0.10159259342689163	No Hit
GACTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	446	0.1004662897303629	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.252607393057464E-4	0.0	0.0	0.9341562859009304	0.0
2	2.252607393057464E-4	0.0	0.0	5.325389137927151	0.0
3	2.252607393057464E-4	0.0	0.0	6.424436284999888	0.0
4	2.252607393057464E-4	0.0	0.0	9.647241682247202	0.0
5	2.252607393057464E-4	0.0	0.0	17.536773815691664	0.0
6	2.252607393057464E-4	0.0	0.0	18.861757484288063	0.0
7	2.252607393057464E-4	0.0	0.0	21.12269952469984	0.0
8	2.252607393057464E-4	0.0	0.0	23.887324578199266	0.0
9	2.252607393057464E-4	0.0	0.0	24.46264050638614	0.0
10	2.252607393057464E-4	0.0	0.0	32.726555988556754	0.0
11	2.252607393057464E-4	0.0	0.0	35.1861780010362	0.0
12	2.252607393057464E-4	0.0	0.0	45.817583853310204	0.0
13	2.252607393057464E-4	0.0	0.0	46.934877120266705	0.0
14	2.252607393057464E-4	0.0	0.0	47.50343522627441	0.0
15	2.252607393057464E-4	0.0	0.0	49.642060685243166	0.0
16	2.252607393057464E-4	0.0	0.0	50.93145315702926	0.0
17	2.252607393057464E-4	0.0	0.0	52.1167751672561	0.0
18	2.252607393057464E-4	0.0	0.0	53.09981303358638	0.0
19	2.252607393057464E-4	0.0	0.0	56.87270515621832	0.0
20	2.252607393057464E-4	0.0	0.0	57.894713130448494	0.0
21	4.505214786114928E-4	0.0	0.0	58.8631090487239	0.0
22	6.757822179172393E-4	0.0	0.0	59.93805329669092	0.0
23	6.757822179172393E-4	0.0	0.0	60.96524226792512	0.0
24	6.757822179172393E-4	0.0	0.0	61.72166783051382	0.0
25	6.757822179172393E-4	0.0	0.0	62.335052823643366	0.0
26	6.757822179172393E-4	0.0	0.0	62.92118126731692	0.0
27	6.757822179172393E-4	0.0	0.0	63.48568468001712	0.0
28	6.757822179172393E-4	0.0	0.0	64.06257743337913	0.0
29	6.757822179172393E-4	0.0	0.0	64.64149753339491	0.0
30	6.757822179172393E-4	0.0	0.0	65.25443200504584	0.0
31	6.757822179172393E-4	0.0	0.0	65.78807469646115	0.0
32	6.757822179172393E-4	0.0	0.0	66.22620683441083	0.0
33	6.757822179172393E-4	0.0	0.0	66.69249656477372	0.0
34	6.757822179172393E-4	0.0	0.0	67.14639695447481	0.0
35	6.757822179172393E-4	0.0	0.0	67.6559367467844	0.0
36	6.757822179172393E-4	0.0	0.0	68.14475255107787	0.0
37	6.757822179172393E-4	0.0	0.0	68.565539612101	0.0
38	6.757822179172393E-4	0.0	0.0	69.01561056923389	0.0
39	6.757822179172393E-4	0.0	0.0	69.46432996193093	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTTCG	55	1.8189894E-12	45.000004	12
ATGGGCG	25	3.8869235E-5	45.0	5
ACCGGTC	25	3.8869235E-5	45.0	17
CCGGGAT	35	1.2096825E-7	45.0	5
GACGTGT	20	7.0286036E-4	45.0	26
TATGGGA	35	1.2096825E-7	45.0	4
TAGCGGT	20	7.0286036E-4	45.0	3
CCTCGCT	20	7.0286036E-4	45.0	26
CGTTAGG	20	7.0286036E-4	45.0	2
AGCGGTA	20	7.0286036E-4	45.0	4
ACGATAC	20	7.0286036E-4	45.0	3
GTTAAAT	20	7.0286036E-4	45.0	10
AACGCTT	20	7.0286036E-4	45.0	19
ACGTGTA	20	7.0286036E-4	45.0	27
GCCCTCG	20	7.0286036E-4	45.0	24
GTCGTTG	20	7.0286036E-4	45.0	1
TCGTCCG	20	7.0286036E-4	45.0	1
GGTATAT	20	7.0286036E-4	45.0	8
AACGATA	20	7.0286036E-4	45.0	2
CGGGATC	35	1.2096825E-7	45.0	6
>>END_MODULE