SRR2933849.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933849.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322783
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	29519	9.14515324536918	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	24746	7.666450835390959	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	20839	6.456040126028942	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	9556	2.9605028765455432	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTCAGT	9357	2.89885155042242	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTA	5911	1.8312612498179892	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	5816	1.8018297122215234	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTC	5117	1.5852755566433179	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCT	4367	1.3529213124606934	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	2828	0.8761304033979486	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2485	0.7698670623917616	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGT	2126	0.6586468308430121	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2107	0.652760523323719	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATG	1938	0.600403366967901	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTAT	1425	0.44147306394698604	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACAGTCAG	1169	0.36216281526598365	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTT	1119	0.34667253232047535	No Hit
TGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCTT	999	0.3094958532512555	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	805	0.24939355542268332	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	753	0.23328366115935475	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.1883618406173807	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACAGTCAGTCG	599	0.18557358968718923	No Hit
TCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	585	0.1812363104624469	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	562	0.1741107803075131	No Hit
TTCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	432	0.13383604464919155	No Hit
TGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	424	0.13135759937791025	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	404	0.12516148619970693	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGT	398	0.12330265224624593	No Hit
GAATCTGTCTCTTATACACATCTGCCGCACAGTCAGTCGTATGCCGTCTTC	361	0.11183984286656981	No Hit
GACTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	358	0.1109104258898393	No Hit
TTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	356	0.11029081457201897	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	336	0.10409470139381566	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	334	0.10347509007599533	No Hit
GAATGCTGCCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTT	330	0.10223586744035466	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCC	35	1.2083001E-7	45.000004	4
CGGCGAT	70	0.0	45.000004	31
TCGTTCG	30	2.1606338E-6	45.000004	1
TCTCTAC	35	1.2083001E-7	45.000004	25
CGACGAA	35	1.2083001E-7	45.000004	19
ACTGACG	35	1.2083001E-7	45.000004	43
GGATCGT	30	2.1606338E-6	45.000004	8
ATATGGA	70	0.0	45.000004	10
AGGCGAC	35	1.2083001E-7	45.000004	31
GCTTGCG	30	2.1606338E-6	45.000004	1
AATGCTC	60	0.0	45.000004	2
AATTATC	35	1.2083001E-7	45.000004	45
TAAGGCG	30	2.1606338E-6	45.000004	29
CCGGTGA	70	0.0	45.000004	18
TGGGCTC	30	2.1606338E-6	45.000004	6
CAGTCGA	30	2.1606338E-6	45.000004	41
TTGTTCG	35	1.2083001E-7	45.000004	1
CTCTACG	35	1.2083001E-7	45.000004	26
AGTCGAA	30	2.1606338E-6	45.000004	42
GTTTGCG	25	3.884552E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content