##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933849.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322783
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.368888696120923	25.0	16.0	31.0	16.0	33.0
2	24.088093239111107	25.0	16.0	31.0	16.0	34.0
3	25.320131481521642	28.0	16.0	31.0	16.0	34.0
4	30.278007206079625	32.0	28.0	35.0	19.0	37.0
5	32.93986672160554	35.0	32.0	35.0	28.0	37.0
6	30.792535542454218	35.0	28.0	35.0	17.0	37.0
7	33.00493520414644	33.0	32.0	35.0	28.0	37.0
8	31.066047468423058	35.0	28.0	35.0	17.0	37.0
9	34.00564465910534	35.0	32.0	37.0	28.0	39.0
10	33.57720511922871	35.0	32.0	37.0	28.0	39.0
11	34.216129721825496	35.0	32.0	38.0	27.0	39.0
12	34.48273298160064	35.0	34.0	39.0	27.0	39.0
13	34.62445048221251	35.0	33.0	38.0	30.0	39.0
14	34.44838173014068	36.0	33.0	38.0	27.0	40.0
15	35.13190595539418	37.0	33.0	39.0	27.0	40.0
16	34.746235086730096	36.0	32.0	38.0	27.0	40.0
17	34.09638363854354	36.0	32.0	38.0	27.0	40.0
18	34.42645368560302	36.0	32.0	38.0	27.0	40.0
19	34.11302949659679	36.0	32.0	39.0	27.0	40.0
20	33.552377293723644	35.0	31.0	38.0	25.0	40.0
21	33.88143737433508	36.0	32.0	39.0	25.0	40.0
22	35.30804286471097	37.0	34.0	39.0	29.0	40.0
23	35.3368857715555	37.0	33.0	39.0	28.0	40.0
24	35.79407837463559	37.0	34.0	39.0	30.0	40.0
25	33.58933401077504	36.0	32.0	39.0	24.0	40.0
26	34.834195109407865	37.0	33.0	39.0	27.0	40.0
27	34.8087166920191	37.0	33.0	39.0	27.0	40.0
28	34.75968375038338	37.0	33.0	39.0	27.0	40.0
29	35.206724022020985	37.0	33.0	39.0	27.0	40.0
30	33.5304740336387	36.0	31.0	39.0	23.0	40.0
31	34.229652738836926	36.0	32.0	39.0	25.0	40.0
32	34.49960809584148	36.0	33.0	39.0	27.0	40.0
33	34.75074895518041	37.0	33.0	39.0	27.0	40.0
34	34.904632524017686	37.0	34.0	39.0	26.0	40.0
35	35.136060449280166	37.0	34.0	40.0	27.0	40.0
36	35.356149487426535	38.0	34.0	40.0	29.0	40.0
37	35.783857885948144	38.0	35.0	40.0	30.0	40.0
38	34.80095296220681	37.0	33.0	39.0	26.0	40.0
39	35.02708940681511	37.0	34.0	39.0	28.0	40.0
40	34.18982722138403	37.0	33.0	39.0	24.0	40.0
41	34.0717509906036	36.0	33.0	39.0	25.0	40.0
42	34.869894015484086	37.0	33.0	39.0	28.0	40.0
43	34.95142866879606	37.0	34.0	40.0	28.0	40.0
44	35.72066062958706	38.0	35.0	40.0	30.0	40.0
45	35.98139617018244	38.0	35.0	40.0	31.0	40.0
46	35.72995170129778	38.0	35.0	40.0	30.0	41.0
47	34.7653934686771	37.0	33.0	40.0	25.0	41.0
48	35.36172908734351	38.0	34.0	40.0	28.0	41.0
49	35.79739949129911	38.0	35.0	40.0	30.0	41.0
50	35.28944523100659	38.0	34.0	40.0	28.0	41.0
51	34.90991161244551	37.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	8.0
13	9.0
14	14.0
15	26.0
16	64.0
17	187.0
18	489.0
19	1080.0
20	1504.0
21	1743.0
22	1774.0
23	1936.0
24	2292.0
25	2975.0
26	4084.0
27	5575.0
28	7711.0
29	10416.0
30	13998.0
31	18940.0
32	25966.0
33	34146.0
34	41149.0
35	46482.0
36	45432.0
37	35835.0
38	17344.0
39	1596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.14680450953117	2.7820548170132877	28.326151005474266	10.744989667981276
2	11.965623964087328	44.70898405430274	27.58540567502006	15.739986306589875
3	11.03465795906228	44.71363113918639	31.184418014579457	13.067292887171877
4	13.492656056855534	2.6063950084112237	67.26190660598607	16.639042328747177
5	43.61444066137312	3.0791584439081365	31.945300712862824	21.36110018185592
6	13.260921423990732	35.80083213799983	35.59976826536714	15.3384781726423
7	47.09851510147685	1.305521046647438	45.86827682994458	5.727687021931143
8	39.281808521514456	30.796541329623928	16.932738093394015	12.988912055467605
9	37.060501947128564	3.1882100358445147	16.84785134285263	42.90343667417429
10	39.657292980113574	17.137519633933636	24.324701114990567	18.880486270962226
11	35.44083796234622	17.576824058268247	28.01789437485865	18.96444360452688
12	21.579822977046497	12.66206708531738	31.90905345077033	33.84905648686579
13	21.76849462332279	20.269035234197588	45.185155352047666	12.777314790431962
14	18.583382644067374	33.77470312872735	21.099624205735743	26.542290021469533
15	7.650650746786542	21.947252488513953	49.22687997818968	21.175216786509825
16	8.063621690113791	24.660220643590275	23.41511170043032	43.861045965865614
17	9.806278521483474	29.58706003723864	36.98428975503666	23.62237168624122
18	9.35055439722662	23.86092204360205	33.872911522601875	32.915612036569456
19	10.67807164565668	28.27379384911845	27.963988190208283	33.08414631501659
20	14.26809962110768	25.063897417150223	46.627920305592305	14.040082656149796
21	18.676634147399337	38.06706053292769	25.88085493969633	17.37545037997664
22	10.870151154180983	21.037972879612617	35.86465210373533	32.22722386247107
23	14.991805640321825	36.0619983084611	27.454048075642152	21.49214797557492
24	21.76911423464061	21.556277746969325	22.55075391207096	34.12385410631911
25	9.934228258613372	44.18448307376783	18.625826019338067	27.25546264828073
26	12.660208251363919	24.957324270485124	30.009634955992105	32.37283252215885
27	20.967646995040013	30.49014353296177	18.7760817639095	29.766127708088714
28	9.054070381649591	32.67644206789081	34.23972142275151	24.02976612770809
29	22.96062679880911	24.636365607854195	21.84408720409687	30.55892038923983
30	12.252813809897052	33.53987043927344	29.54647549592141	24.660840254908095
31	23.241620531440628	28.754612231747025	15.102406260552756	32.90136097625959
32	29.71655880266309	37.79071388517983	16.225761579761016	16.26696573239607
33	18.043391380586957	18.376432463915386	22.804174941059475	40.77600121443818
34	24.456988131345206	32.21947872099832	18.47649969174337	24.847033455913106
35	18.111238819888285	26.097409095274532	18.53102548771156	37.26032659712562
36	22.398949139204976	38.08936654036923	18.192098096863837	21.31958622356196
37	21.52560698673722	19.55865085831658	29.08672389809872	29.82901825684748
38	19.78666782327446	32.95619657788669	23.17191425818584	24.08522134065301
39	32.907557089437795	21.397967055266232	22.676225203929576	23.018250651366397
40	15.926489313253796	26.348351678991765	35.534399271337094	22.190759736417345
41	24.121468602745498	25.840890009696913	16.730125192466765	33.30751619509082
42	19.84336225885502	26.278025794419165	31.623722438914072	22.25488950781175
43	34.94359987979541	19.963566854512166	19.342096702738374	25.750736562954057
44	21.219828801392886	16.08294117100343	31.485858920699044	31.21137110690464
45	16.448202042858515	18.785375933676804	26.389555831626822	38.37686619183786
46	37.86475743765936	23.88446727367922	21.697858933091272	16.55291635557015
47	12.09915020307761	15.587252116747162	52.18800246605304	20.12559521412218
48	21.366986489375215	23.200726184464486	22.34535276021352	33.08693456594678
49	17.54057679617576	15.864528181471762	43.22439533680522	23.370499685547255
50	29.3999374192569	13.31668644259456	29.38382752499357	27.899548613154966
51	19.738647946143384	14.086863310645231	25.611943627762308	40.562545115449076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	98.0
1	123.0
2	148.0
3	585.0
4	1022.0
5	760.5
6	499.0
7	653.5
8	808.0
9	1020.0
10	1232.0
11	1291.0
12	1350.0
13	1296.5
14	1243.0
15	1116.5
16	990.0
17	944.5
18	899.0
19	934.0
20	969.0
21	1000.0
22	1031.0
23	1101.5
24	1172.0
25	1166.0
26	1294.5
27	1429.0
28	1515.0
29	1601.0
30	1955.5
31	2310.0
32	2575.5
33	2841.0
34	3016.0
35	3191.0
36	3508.0
37	3825.0
38	4277.5
39	4730.0
40	5531.5
41	6333.0
42	7712.5
43	9092.0
44	11425.0
45	13758.0
46	67745.0
47	121732.0
48	86348.0
49	50964.0
50	44903.5
51	38843.0
52	27033.0
53	15223.0
54	12642.5
55	10062.0
56	8475.5
57	6889.0
58	6255.5
59	5622.0
60	5128.0
61	4634.0
62	3841.0
63	3048.0
64	2435.0
65	1822.0
66	1386.5
67	951.0
68	703.0
69	455.0
70	382.0
71	309.0
72	275.0
73	241.0
74	191.0
75	97.5
76	54.0
77	43.0
78	32.0
79	28.0
80	24.0
81	14.0
82	4.0
83	2.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	322783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.22117335795256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63644368546042	17.331457976411397
2	10.710569149078102	4.974239659461619
3	4.1785628518824875	2.9109339711199165
4	2.117298609814019	1.9666463227617317
5	1.3274808549243537	1.5412831530780742
6	0.8792059129599487	1.224971575330795
7	0.6203805000400245	1.0084174197525893
8	0.5029751580969661	0.9343738672730596
9	0.42426021293059746	0.8866637958008942
>10	4.341329348667182	18.324694918877388
>50	0.1280785548469728	2.1553179690380224
>100	0.1013955225871868	4.813140716828333
>500	0.009339061290925101	1.5214555909078236
>1k	0.012007364516903702	6.061037910918481
>5k	0.0066707580649465	11.077720945650793
>10k+	0.0040024548389679	23.267644206789083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	29519	9.14515324536918	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	24746	7.666450835390959	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	20839	6.456040126028942	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	9556	2.9605028765455432	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTCAGT	9357	2.89885155042242	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTA	5911	1.8312612498179892	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	5816	1.8018297122215234	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTC	5117	1.5852755566433179	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCT	4367	1.3529213124606934	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	2828	0.8761304033979486	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2485	0.7698670623917616	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGT	2126	0.6586468308430121	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2107	0.652760523323719	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATG	1938	0.600403366967901	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTAT	1425	0.44147306394698604	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACAGTCAG	1169	0.36216281526598365	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTT	1119	0.34667253232047535	No Hit
TGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCTT	999	0.3094958532512555	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	805	0.24939355542268332	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	753	0.23328366115935475	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.1883618406173807	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACAGTCAGTCG	599	0.18557358968718923	No Hit
TCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	585	0.1812363104624469	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	562	0.1741107803075131	No Hit
TTCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	432	0.13383604464919155	No Hit
TGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	424	0.13135759937791025	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	404	0.12516148619970693	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGT	398	0.12330265224624593	No Hit
GAATCTGTCTCTTATACACATCTGCCGCACAGTCAGTCGTATGCCGTCTTC	361	0.11183984286656981	No Hit
GACTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	358	0.1109104258898393	No Hit
TTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	356	0.11029081457201897	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	336	0.10409470139381566	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	334	0.10347509007599533	No Hit
GAATGCTGCCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTT	330	0.10223586744035466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0016016952565656	0.0
2	0.0	0.0	0.0	5.749063612395944	0.0
3	0.0	0.0	0.0	6.897513189975928	0.0
4	0.0	0.0	0.0	10.262932062717057	0.0
5	0.0	0.0	0.0	19.181927177081818	0.0
6	0.0	0.0	0.0	20.476914831326308	0.0
7	0.0	0.0	0.0	22.753057007339297	0.0
8	0.0	0.0	0.0	25.68072048404036	0.0
9	0.0	0.0	0.0	26.23899028139648	0.0
10	0.0	0.0	0.0	35.48483036591146	0.0
11	0.0	0.0	0.0	37.399739143635195	0.0
12	0.0	0.0	0.0	49.08932626563357	0.0
13	0.0	0.0	0.0	50.25388573747688	0.0
14	0.0	0.0	0.0	50.909434511730794	0.0
15	0.0	0.0	0.0	53.215627836658065	0.0
16	0.0	0.0	0.0	54.53075285873172	0.0
17	0.0	0.0	0.0	55.44808741476472	0.0
18	0.0	0.0	0.0	56.26349590901627	0.0
19	0.0	0.0	0.0	60.24976532221338	0.0
20	0.0	0.0	0.0	61.21016286483489	0.0
21	0.0	0.0	0.0	61.87872347676303	0.0
22	0.0	0.0	0.0	62.68793585783638	0.0
23	0.0	0.0	0.0	63.30971581526908	0.0
24	0.0	0.0	0.0	63.81531865061047	0.0
25	0.0	0.0	0.0	64.21775620153478	0.0
26	0.0	0.0	0.0	64.63537422974568	0.0
27	0.0	0.0	0.0	65.03378430710416	0.0
28	0.0	0.0	0.0	65.33615463020047	0.0
29	0.0	0.0	0.0	65.70203511337338	0.0
30	0.0	0.0	0.0	66.09517849453039	0.0
31	0.0	0.0	0.0	66.47809828894334	0.0
32	0.0	0.0	0.0	66.799986368551	0.0
33	0.0	0.0	0.0	67.10173708032951	0.0
34	0.0	0.0	0.0	67.40968390528622	0.0
35	0.0	0.0	0.0	67.73962693202554	0.0
36	0.0	0.0	0.0	68.0921857718653	0.0
37	0.0	0.0	0.0	68.41717190806207	0.0
38	0.0	0.0	0.0	68.72078145379403	0.0
39	0.0	0.0	0.0	69.04483817301407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCC	35	1.2083001E-7	45.000004	4
CGGCGAT	70	0.0	45.000004	31
TCGTTCG	30	2.1606338E-6	45.000004	1
TCTCTAC	35	1.2083001E-7	45.000004	25
CGACGAA	35	1.2083001E-7	45.000004	19
ACTGACG	35	1.2083001E-7	45.000004	43
GGATCGT	30	2.1606338E-6	45.000004	8
ATATGGA	70	0.0	45.000004	10
AGGCGAC	35	1.2083001E-7	45.000004	31
GCTTGCG	30	2.1606338E-6	45.000004	1
AATGCTC	60	0.0	45.000004	2
AATTATC	35	1.2083001E-7	45.000004	45
TAAGGCG	30	2.1606338E-6	45.000004	29
CCGGTGA	70	0.0	45.000004	18
TGGGCTC	30	2.1606338E-6	45.000004	6
CAGTCGA	30	2.1606338E-6	45.000004	41
TTGTTCG	35	1.2083001E-7	45.000004	1
CTCTACG	35	1.2083001E-7	45.000004	26
AGTCGAA	30	2.1606338E-6	45.000004	42
GTTTGCG	25	3.884552E-5	45.0	1
>>END_MODULE