SRR2933847.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933847.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425008
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	29755	7.0010446862176705	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCG	24767	5.827419719158228	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTC	17888	4.2088619508338665	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACCGCAT	9436	2.2201935022399577	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	8437	1.9851391032639387	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATACCGCATCGTA	5609	1.3197398637202125	No Hit
GAACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCT	4555	1.0717445318676355	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTC	4311	1.0143338478334525	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCT	4137	0.9733934420057976	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	2330	0.5482249745887137	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	2313	0.5442250498814141	No Hit
TCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	2026	0.4766969092346497	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATACCGCATCGT	1959	0.46093250009411585	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATACCGCATCGTATG	1773	0.41716861800248467	No Hit
TGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCTT	1337	0.3145823137446825	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATACCGCATCGTAT	1287	0.30281782931144824	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATACCGCA	1155	0.27175959040770997	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT	1082	0.25458344313518805	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1024	0.24093664119263636	No Hit
CGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	741	0.1743496593005308	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCATACCGCATCG	560	0.131762225652223	No Hit
TCCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	540	0.12705643187892934	No Hit
TGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	525	0.12352708654895907	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	516	0.12140947935097691	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	447	0.10517449083311374	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	125	0.0	45.000004	17
GTTTGCG	80	0.0	45.0	1
GATACGT	20	7.028264E-4	45.0	5
CCGGGCT	45	3.8380676E-10	45.0	5
CGAGTGA	20	7.028264E-4	45.0	16
TTTCGTC	40	6.7975634E-9	45.0	16
GGCCGGT	35	1.2095188E-7	45.0	9
GGTCCGT	20	7.028264E-4	45.0	8
AAGCGCA	20	7.028264E-4	45.0	35
CATTAGT	20	7.028264E-4	45.0	21
TACGCTC	20	7.028264E-4	45.0	17
TCGCTCG	20	7.028264E-4	45.0	1
TTGCCCG	25	3.8866427E-5	45.0	1
GGCGCGT	20	7.028264E-4	45.0	9
ATCGGAC	20	7.028264E-4	45.0	27
CACCGGT	140	0.0	45.0	16
TAAGCGC	20	7.028264E-4	45.0	34
TGTGTCG	20	7.028264E-4	45.0	1
TTATTCC	20	7.028264E-4	45.0	37
CGGAGAC	20	7.028264E-4	45.0	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content