SRR2933846.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933846.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682141
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	34467	5.0527676829277235	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	28009	4.106042592367267	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC	20669	3.030018720469815	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAACCGATT	10492	1.5380984283307997	No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	9504	1.3932603376721235	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTA	6600	0.967541901161197	No Hit
GAACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT	5691	0.8342849938649046	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTC	4952	0.7259496203864011	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT	4667	0.6841694019271675	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	2792	0.4092995436427366	No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2766	0.4054880149411925	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAACCGATTCGT	2280	0.334241747673868	No Hit
TCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2216	0.32485952317775946	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATG	1997	0.2927547237301379	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1913	0.2804405540789954	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTAT	1500	0.21989588662754475	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTT	1432	0.20992727310042936	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAACCGAT	1245	0.18251358590086214	No Hit
TGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCTT	968	0.1419061455036422	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTG	925	0.13560246342031926	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGG	20	7.0312666E-4	45.000004	2
CCCTCGA	40	6.8084773E-9	45.000004	41
TGCGACG	20	7.0312666E-4	45.000004	1
GCCCTCG	20	7.0312666E-4	45.000004	13
GCGATAC	40	6.8084773E-9	45.000004	33
CCCGGTT	20	7.0312666E-4	45.000004	28
ACCGTAT	25	3.8891318E-5	45.0	12
TACGGCT	3945	0.0	44.14449	7
ACCGGTG	190	0.0	43.81579	17
ACGGCTG	3985	0.0	43.757843	8
TGATACC	3030	0.0	43.73762	4
GATACCT	3075	0.0	43.243904	5
GGCGACT	870	0.0	42.672417	10
CCGGTGA	185	0.0	42.567566	18
CGGCTGT	4195	0.0	42.42551	9
CGTTTTT	1215	0.0	41.666664	1
TTCGGGC	200	0.0	41.625	4
GAATGAC	650	0.0	41.538464	1
GCGACTG	895	0.0	41.480446	11
CTTTTCG	60	3.6379788E-12	41.249996	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content