##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933846.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682141
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.707136794299124	30.0	16.0	33.0	16.0	34.0
2	26.764872658292052	31.0	16.0	33.0	16.0	34.0
3	27.840661974577102	31.0	25.0	34.0	16.0	34.0
4	31.74646590660875	35.0	28.0	35.0	19.0	37.0
5	33.422453715580794	35.0	33.0	37.0	28.0	37.0
6	32.04246922557066	35.0	32.0	37.0	17.0	37.0
7	33.16820862548945	35.0	32.0	35.0	28.0	37.0
8	32.56220488139549	35.0	32.0	37.0	25.0	37.0
9	34.320441081829124	35.0	32.0	39.0	28.0	39.0
10	33.277205152600416	35.0	31.0	37.0	27.0	39.0
11	33.92067182591283	35.0	32.0	38.0	27.0	39.0
12	34.23921007533633	35.0	32.0	39.0	27.0	39.0
13	34.47284505696037	35.0	33.0	38.0	27.0	39.0
14	34.41372384888168	37.0	32.0	39.0	26.0	40.0
15	34.993428044934994	37.0	33.0	39.0	27.0	40.0
16	34.79750374189501	36.0	32.0	39.0	27.0	40.0
17	34.17332047186726	36.0	32.0	38.0	27.0	40.0
18	34.425778541386606	36.0	32.0	39.0	27.0	40.0
19	34.27103194207649	36.0	32.0	39.0	27.0	40.0
20	34.3633075859683	36.0	32.0	39.0	27.0	40.0
21	34.00048083900543	36.0	32.0	39.0	25.0	40.0
22	35.08338012229143	37.0	33.0	39.0	27.0	40.0
23	35.61555895335422	37.0	34.0	39.0	29.0	40.0
24	35.870092840043334	38.0	34.0	40.0	30.0	40.0
25	33.80458438944441	36.0	32.0	39.0	24.0	40.0
26	34.65335612432034	37.0	33.0	39.0	27.0	40.0
27	35.01007709549785	37.0	33.0	39.0	27.0	40.0
28	35.039647228358945	37.0	33.0	39.0	27.0	40.0
29	35.5823649362815	38.0	34.0	40.0	29.0	40.0
30	33.35112242190397	36.0	31.0	39.0	23.0	40.0
31	34.25265450984474	36.0	32.0	39.0	25.0	40.0
32	34.57631926537182	37.0	33.0	39.0	27.0	40.0
33	35.28326548323587	38.0	34.0	39.0	28.0	40.0
34	35.62913825733976	38.0	35.0	40.0	30.0	41.0
35	35.49357684115161	38.0	34.0	40.0	28.0	41.0
36	35.550991657150064	38.0	35.0	40.0	28.0	41.0
37	35.81276305045438	38.0	35.0	40.0	30.0	41.0
38	35.42420262086577	38.0	34.0	40.0	29.0	41.0
39	35.4223818829245	38.0	34.0	40.0	29.0	41.0
40	34.93954182493062	37.0	33.0	40.0	26.0	41.0
41	34.515076794973474	37.0	33.0	39.0	25.0	40.0
42	35.137351368705296	37.0	34.0	40.0	27.0	41.0
43	35.30762701552905	38.0	34.0	40.0	28.0	41.0
44	35.97538778639607	38.0	35.0	40.0	30.0	41.0
45	36.20027970756779	38.0	35.0	40.0	31.0	41.0
46	35.90153355391334	38.0	35.0	40.0	30.0	41.0
47	35.48155733198855	38.0	34.0	40.0	28.0	41.0
48	35.7248999840209	38.0	35.0	40.0	29.0	41.0
49	35.89873208031771	38.0	35.0	40.0	30.0	41.0
50	35.57160323158995	38.0	35.0	40.0	28.0	41.0
51	35.27294943420788	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	9.0
11	12.0
12	16.0
13	23.0
14	38.0
15	51.0
16	132.0
17	377.0
18	1030.0
19	1948.0
20	2704.0
21	3470.0
22	3622.0
23	3926.0
24	4753.0
25	6170.0
26	8406.0
27	11190.0
28	14679.0
29	19460.0
30	26399.0
31	35315.0
32	47718.0
33	61708.0
34	74244.0
35	89316.0
36	99265.0
37	99485.0
38	59696.0
39	6970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.405076076646914	3.2921346173298485	40.920132348004294	14.382656958018943
2	16.173049266940414	25.670059415868568	40.28756518080573	17.869326136385293
3	15.99566658506086	25.644698090277522	41.348342937897	17.011292386764612
4	16.219227403132198	3.566124892067769	60.0950536619262	20.119594042873835
5	33.218059022987916	4.543342212240578	39.814789024556504	22.423809740215
6	17.752927913730446	22.657485769071204	43.1325781619929	16.45700815520545
7	65.05546507247035	1.8076907853361694	28.48912468243369	4.647719459759786
8	62.23815897299825	18.659338758409184	11.323758577772045	7.778743690820519
9	58.608704065581755	3.9356965788597957	11.897393647354432	25.558205708204024
10	41.82610340090978	20.46395100133257	20.257542062418178	17.452403535339467
11	32.42232910791171	22.270615605864478	26.548763378832234	18.75829190739158
12	24.905994508466726	16.872904575447013	29.68682427826505	28.534276637821215
13	24.71116675291472	19.992347623145363	38.14973737101274	17.14674825292718
14	20.542380534229725	27.988201852696143	25.84509654162409	25.624321071450034
15	14.278866099530743	23.52372896512598	40.76327328220998	21.434131653133296
16	16.26086102433368	24.725973075947643	26.417852027659972	32.5953138720587
17	16.93315604838296	27.99069400607792	33.82731722620397	21.248832719335155
18	15.919142816514476	24.61162721490132	31.789615343455385	27.679614625128824
19	17.206120142316617	27.75965672786125	28.17115523037026	26.86306789945187
20	20.72137578594455	25.53973445372731	38.17114057064448	15.567749189683658
21	22.277065885205552	32.45076897591554	27.26679674730004	18.005368391578866
22	17.736655618120007	21.731137697338234	34.01232296548661	26.519883719055155
23	19.54566577877594	30.608041445976713	28.651114652249316	21.195178122998033
24	21.915117255816615	23.270848695504302	26.14356855840655	28.67046549027254
25	16.200169759624476	34.838838304690675	22.979559944351678	25.98143199133317
26	16.92201465679383	23.75579242414691	30.35003027233373	28.97216264672553
27	20.754799960711935	30.609507418554227	24.26697706192708	24.36871555880676
28	14.32958875071283	28.71195251421627	32.895838250449685	24.062620484621213
29	20.487113368057337	21.87245745381087	25.421723661237195	32.2187055168946
30	17.19497875072749	33.61401821617525	26.826271987756193	22.364731045341067
31	22.998031198828393	26.71046015413236	21.54598536079784	28.745523286241408
32	22.234259485942058	31.956296425519064	20.90887367860897	24.900570409929912
33	19.214209378999357	23.25677535876014	23.20150819258775	34.32750706965276
34	25.679588237622426	24.586119292052523	23.21968625254896	26.514606217776088
35	17.736802215377757	30.054490200706308	23.65346753823623	28.555240045679703
36	17.069344900834285	29.59520099217024	25.03104196932892	28.304412137666553
37	17.06392080229747	30.2529828877021	28.321417419565748	24.361678890434675
38	18.07734764513495	30.596900054387582	21.793588129140456	29.532164171337012
39	24.3983282048726	22.84381088367361	25.402666017729477	27.355194893724317
40	19.067905315763163	26.00048963484089	25.93144232643984	29.0001627229561
41	22.998617587859403	26.337076938638788	27.35255614308479	23.311749330417026
42	17.920488579340635	27.43039928695094	30.533716636296603	24.115395497411825
43	20.450757248134916	21.585420023133047	29.276498553818055	28.687324174913986
44	21.052099199432377	22.59576832355774	29.696059905503407	26.656072571506478
45	19.171696174251366	21.818656260215995	26.418145222175475	32.59150234335716
46	26.183003220741753	25.64367190947326	25.003481684871602	23.169843184913383
47	14.734636973880766	21.881839678306978	39.3298452959139	24.053678051898363
48	22.05936895744428	24.826538794765305	25.807567643639658	27.306524604150756
49	19.005015092187687	20.603804785227688	35.879825431985466	24.51135469059916
50	22.47585176671685	19.24821994279775	30.29842803760513	27.977500252880272
51	19.286335229813194	19.00985280169349	26.973895426312154	34.72991654218116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	324.5
2	562.0
3	2043.0
4	3524.0
5	2474.0
6	1424.0
7	1469.0
8	1514.0
9	1569.0
10	1624.0
11	1657.5
12	1691.0
13	1633.0
14	1575.0
15	1540.5
16	1506.0
17	1454.0
18	1402.0
19	1495.0
20	1588.0
21	1763.0
22	1938.0
23	2232.0
24	2526.0
25	2817.5
26	4111.5
27	5114.0
28	6147.5
29	7181.0
30	8165.5
31	9150.0
32	10627.0
33	12104.0
34	13398.0
35	14692.0
36	15938.5
37	17185.0
38	18755.5
39	20326.0
40	21804.5
41	23283.0
42	25122.5
43	26962.0
44	31427.5
45	35893.0
46	96543.5
47	157194.0
48	120067.5
49	82941.0
50	76092.0
51	69243.0
52	55861.5
53	42480.0
54	36854.5
55	31229.0
56	28617.5
57	26006.0
58	24129.5
59	22253.0
60	20741.5
61	19230.0
62	16143.5
63	13057.0
64	10593.0
65	8129.0
66	6814.5
67	5500.0
68	4472.5
69	3445.0
70	2893.5
71	2342.0
72	1803.5
73	1265.0
74	1070.5
75	679.0
76	482.0
77	342.5
78	203.0
79	183.0
80	163.0
81	115.0
82	67.0
83	46.5
84	26.0
85	36.5
86	47.0
87	24.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	682141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.785578314217812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75768049103746	21.231574880498144
2	10.558152719493151	6.078450639208838
3	3.7526510426443047	3.240666914219063
4	1.87585601273021	2.1599040064256734
5	1.1117029420958973	1.6000506050923902
6	0.8193227096560337	1.4150806814052543
7	0.6469086072692136	1.3035146863682667
8	0.5458311186658651	1.2569651530154702
9	0.511248401020852	1.3244922797043934
>10	6.262554931429038	33.47538180649469
>50	0.09092817064249938	1.8106300124972718
>100	0.054246919985572004	3.1065503351991977
>500	0.003616461332371467	0.7016470142883725
>1k	0.005683010665155162	4.128385145537351
>5k	0.0015499119995877714	3.2412879771969223
>10k+	0.0020665493327836956	13.925417862848736
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	34467	5.0527676829277235	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	28009	4.106042592367267	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC	20669	3.030018720469815	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAACCGATT	10492	1.5380984283307997	No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	9504	1.3932603376721235	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTA	6600	0.967541901161197	No Hit
GAACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT	5691	0.8342849938649046	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTC	4952	0.7259496203864011	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT	4667	0.6841694019271675	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	2792	0.4092995436427366	No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2766	0.4054880149411925	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAACCGATTCGT	2280	0.334241747673868	No Hit
TCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2216	0.32485952317775946	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATG	1997	0.2927547237301379	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1913	0.2804405540789954	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTAT	1500	0.21989588662754475	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTT	1432	0.20992727310042936	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAACCGAT	1245	0.18251358590086214	No Hit
TGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCTT	968	0.1419061455036422	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTG	925	0.13560246342031926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4647133070728779	0.0
2	0.0	0.0	0.0	2.7731803248888425	0.0
3	0.0	0.0	0.0	3.369098177649489	0.0
4	0.0	0.0	0.0	5.053940460989737	0.0
5	0.0	0.0	0.0	9.502434247464967	0.0
6	0.0	0.0	0.0	10.293473050293121	0.0
7	0.0	0.0	0.0	11.61885885762621	0.0
8	0.0	0.0	0.0	13.242423487226247	0.0
9	0.0	0.0	0.0	13.62372295463841	0.0
10	0.0	0.0	0.0	18.837601023835248	0.0
11	0.0	0.0	0.0	20.382149731507123	0.0
12	1.465972577516965E-4	0.0	0.0	27.12972831130221	0.0
13	1.465972577516965E-4	0.0	0.0	27.859636057647904	0.0
14	1.465972577516965E-4	0.0	0.0	28.240642330544564	0.0
15	1.465972577516965E-4	0.0	0.0	29.55884487224782	0.0
16	1.465972577516965E-4	0.0	0.0	30.42391529024058	0.0
17	1.465972577516965E-4	0.0	0.0	31.197802213911785	0.0
18	1.465972577516965E-4	0.0	0.0	31.914662804317583	0.0
19	1.465972577516965E-4	0.0	0.0	34.25479482980791	0.0
20	1.465972577516965E-4	0.0	0.0	35.058440996802716	0.0
21	1.465972577516965E-4	0.0	0.0	35.84537507641382	0.0
22	1.465972577516965E-4	0.0	0.0	36.71718896826316	0.0
23	1.465972577516965E-4	0.0	0.0	37.481547070180504	0.0
24	1.465972577516965E-4	0.0	0.0	38.10238645675894	0.0
25	1.465972577516965E-4	0.0	0.0	38.68012624955838	0.0
26	1.465972577516965E-4	0.0	0.0	39.20127950086566	0.0
27	1.465972577516965E-4	0.0	0.0	39.70747983188227	0.0
28	1.465972577516965E-4	0.0	0.0	40.2032717575985	0.0
29	1.465972577516965E-4	0.0	0.0	40.68543013834383	0.0
30	1.465972577516965E-4	0.0	0.0	41.24645784375957	0.0
31	1.465972577516965E-4	0.0	0.0	41.75573671718897	0.0
32	1.465972577516965E-4	0.0	0.0	42.260177881112554	0.0
33	1.465972577516965E-4	0.0	0.0	42.75728918214856	0.0
34	1.465972577516965E-4	0.0	0.0	43.25674603930859	0.0
35	1.465972577516965E-4	0.0	0.0	43.732747335228346	0.0
36	1.465972577516965E-4	0.0	0.0	44.251701627669355	0.0
37	1.465972577516965E-4	0.0	0.0	44.759074736747976	0.0
38	1.465972577516965E-4	0.0	0.0	45.24724360506112	0.0
39	1.465972577516965E-4	0.0	0.0	45.742742336261855	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGG	20	7.0312666E-4	45.000004	2
CCCTCGA	40	6.8084773E-9	45.000004	41
TGCGACG	20	7.0312666E-4	45.000004	1
GCCCTCG	20	7.0312666E-4	45.000004	13
GCGATAC	40	6.8084773E-9	45.000004	33
CCCGGTT	20	7.0312666E-4	45.000004	28
ACCGTAT	25	3.8891318E-5	45.0	12
TACGGCT	3945	0.0	44.14449	7
ACCGGTG	190	0.0	43.81579	17
ACGGCTG	3985	0.0	43.757843	8
TGATACC	3030	0.0	43.73762	4
GATACCT	3075	0.0	43.243904	5
GGCGACT	870	0.0	42.672417	10
CCGGTGA	185	0.0	42.567566	18
CGGCTGT	4195	0.0	42.42551	9
CGTTTTT	1215	0.0	41.666664	1
TTCGGGC	200	0.0	41.625	4
GAATGAC	650	0.0	41.538464	1
GCGACTG	895	0.0	41.480446	11
CTTTTCG	60	3.6379788E-12	41.249996	1
>>END_MODULE