SRR2933845.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933845.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881224
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	54874	6.227020598621917	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	43676	4.956288072045246	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	35217	3.996373226330649	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT	17542	1.9906402912312875	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	16948	1.9232340471889102	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA	10936	1.2410011529418172	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	10771	1.2222771962633792	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	8482	0.9625248517970459	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	7232	0.8206766951422114	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	5079	0.5763574301199241	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	4353	0.4939720207347962	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGT	3938	0.4468784327253911	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	3934	0.4464245186240956	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATG	3548	0.40262180784908264	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2501	0.28380979183499316	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTAT	2478	0.2811997857525442	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	2192	0.248744927509918	No Hit
TGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCTT	2048	0.23240401986328105	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATTCTCG	2010	0.2280918359009741	No Hit
CGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1668	0.18928218024021135	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCATTCTCGTCG	1094	0.12414550670431128	No Hit
TCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1045	0.11858505896344175	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	977	0.11086851924141876	No Hit
TGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	974	0.11052808366544715	No Hit
TTCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	968	0.10984721251350393	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	915	0.10383285067133896	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGA	25	3.8900616E-5	45.000004	4
CCGTTCG	25	3.8900616E-5	45.000004	1
GTACGAG	25	3.8900616E-5	45.000004	1
CTCACGT	20	7.03239E-4	45.0	13
GTTTACG	20	7.03239E-4	45.0	1
CGCGCCA	20	7.03239E-4	45.0	41
TTCGTCG	40	6.8121153E-9	45.0	1
GTTAGCG	20	7.03239E-4	45.0	1
CCTCACG	20	7.03239E-4	45.0	12
AACGCAG	40	6.8121153E-9	45.0	42
AGACACG	20	7.03239E-4	45.0	24
CAGTCGA	20	7.03239E-4	45.0	26
ACGAGCG	20	7.03239E-4	45.0	42
TTTTACG	45	3.8380676E-10	45.0	1
TACGGCT	6430	0.0	44.300156	7
ACGGCTG	6435	0.0	44.26573	8
GCGACTG	1465	0.0	43.924915	11
TCGGGAA	205	0.0	43.90244	5
GGCGACT	1490	0.0	43.640938	10
CGTTTTT	1815	0.0	43.512398	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content