##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933845.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881224
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.029505551369457	28.0	16.0	31.0	16.0	34.0
2	25.98228146305593	30.0	16.0	33.0	16.0	34.0
3	27.05788426098245	31.0	23.0	33.0	16.0	34.0
4	31.328649696331468	35.0	28.0	35.0	19.0	37.0
5	33.35437641280764	35.0	32.0	35.0	28.0	37.0
6	31.651931858415114	35.0	28.0	37.0	17.0	37.0
7	33.19886771127432	35.0	32.0	35.0	28.0	37.0
8	32.275652955434715	35.0	32.0	37.0	19.0	37.0
9	34.264938313073635	35.0	32.0	39.0	28.0	39.0
10	33.38694248000508	35.0	32.0	37.0	27.0	39.0
11	34.07607259902136	35.0	32.0	38.0	27.0	39.0
12	34.24648443528547	35.0	32.0	39.0	27.0	39.0
13	34.56183898758999	35.0	33.0	38.0	27.0	39.0
14	34.44935339936271	37.0	32.0	39.0	27.0	40.0
15	35.00939261754105	37.0	33.0	39.0	27.0	40.0
16	34.608882645048254	36.0	32.0	39.0	27.0	40.0
17	34.10062481276044	36.0	32.0	38.0	27.0	40.0
18	34.446983967753944	36.0	32.0	39.0	27.0	40.0
19	34.23581404954926	36.0	32.0	39.0	27.0	40.0
20	34.13350861982878	36.0	32.0	39.0	25.0	40.0
21	33.88912921118808	36.0	32.0	39.0	25.0	40.0
22	35.06843776383757	37.0	33.0	39.0	27.0	40.0
23	35.47571446079544	37.0	34.0	39.0	29.0	40.0
24	35.7380189372963	38.0	34.0	39.0	30.0	40.0
25	33.67009069203744	36.0	32.0	39.0	24.0	40.0
26	34.60166768040816	37.0	33.0	39.0	27.0	40.0
27	34.898875881728145	37.0	33.0	39.0	27.0	40.0
28	35.03414341869944	37.0	33.0	39.0	27.0	40.0
29	35.51724306192296	38.0	34.0	40.0	28.0	40.0
30	33.47716471634908	36.0	31.0	39.0	23.0	40.0
31	34.48025700616415	37.0	33.0	39.0	25.0	40.0
32	34.88838138770619	37.0	33.0	39.0	27.0	40.0
33	35.32678410937514	38.0	34.0	39.0	28.0	40.0
34	35.59449016368143	38.0	35.0	40.0	29.0	40.0
35	35.58192695614282	38.0	35.0	40.0	28.0	41.0
36	35.56492219912304	38.0	35.0	40.0	28.0	41.0
37	35.64697398164371	38.0	35.0	40.0	29.0	41.0
38	35.436158116438044	38.0	34.0	40.0	29.0	41.0
39	35.335738699808445	38.0	34.0	40.0	29.0	41.0
40	34.95087060724629	37.0	33.0	40.0	27.0	40.0
41	34.39606501865587	37.0	33.0	39.0	25.0	40.0
42	35.41743642933012	38.0	35.0	40.0	29.0	40.0
43	35.509839722930835	38.0	35.0	40.0	30.0	40.0
44	36.03560388732036	38.0	35.0	40.0	30.0	41.0
45	36.37124386081178	39.0	35.0	40.0	31.0	41.0
46	36.114532740824124	38.0	35.0	40.0	31.0	41.0
47	35.58556053852369	38.0	35.0	40.0	28.0	41.0
48	35.99457799606002	38.0	35.0	40.0	30.0	41.0
49	36.20846912930197	39.0	35.0	40.0	31.0	41.0
50	35.83654439733825	38.0	35.0	40.0	29.0	41.0
51	35.41421590878142	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	9.0
11	16.0
12	14.0
13	23.0
14	31.0
15	51.0
16	163.0
17	457.0
18	1223.0
19	2462.0
20	3685.0
21	4430.0
22	4812.0
23	4970.0
24	5929.0
25	7961.0
26	10701.0
27	13828.0
28	18241.0
29	24283.0
30	33410.0
31	45445.0
32	62454.0
33	81292.0
34	101681.0
35	123197.0
36	133071.0
37	123899.0
38	67264.0
39	6209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26315216108504	3.238904069793832	39.69796555699799	10.799978212123138
2	15.697484408050622	30.841534048096737	36.042708777790885	17.41827276606175
3	13.984753025337485	30.932543825406476	38.90248109447768	16.180222054778355
4	14.250406253120659	3.185115248790319	63.39738817826115	19.167090319827874
5	35.11354661243906	3.676363784917342	39.861488111989686	21.34860149065391
6	15.530444018773887	26.11696912476283	43.19979936996723	15.152787486496056
7	59.61628371446987	1.373430591994771	34.18154748395414	4.828738209581219
8	56.034334062621994	21.557628934300475	13.191084219222354	9.216952783855183
9	53.28179895236625	2.978130418599584	13.735667662251597	30.004402966782568
10	40.85658130055469	16.200421232286004	23.92592575780959	19.01707170934972
11	32.63914736775212	20.557202255045258	26.53944967454359	20.26420070265903
12	21.043798171634002	15.490726534910534	32.21598594681942	31.249489346636043
13	23.169364429475365	20.067201982696794	41.71481938757909	15.048614200248744
14	21.003059381042732	29.10258912603379	25.94209871723875	23.95225277568473
15	13.078513522101076	22.75403302679001	42.85085290459633	21.31660054651258
16	14.201723965756722	23.909925285738925	25.40489137835556	36.48345937014879
17	15.185015387688033	28.11067333617786	34.46921554565014	22.235095730483962
18	14.91992955253148	23.57323450110301	33.12517589171426	28.38166005465126
19	15.007648452606828	28.71347126269825	28.18284567828384	28.096034606411084
20	17.47194810853994	25.76200829754977	41.41671130155329	15.349332292356992
21	19.646196653745246	34.156241772806915	28.093538078853957	18.104023494593882
22	14.681057256724737	21.636042595299266	35.36501502455675	28.317885123419245
23	17.088163735894618	32.431935580510746	28.602943179032803	21.876957504561837
24	21.28176263923815	23.224401514257444	25.586570497399073	29.907265349105334
25	13.845287917714453	37.30186649478453	21.934718073951686	26.918127513549333
26	15.173327099579675	22.001783882418092	31.671629460840826	31.1532595571614
27	20.16774395613374	29.863689595380972	24.79290169128394	25.17566475720135
28	11.354774722431527	25.582598749012735	37.35599575136401	25.706630777191723
29	18.373875427814042	20.020335351738037	29.36880974644358	32.23697947400434
30	14.698079035523318	32.794272511869856	30.952402567338154	21.555245885268672
31	19.149387669877353	20.597714088585874	31.79373235408931	28.45916588744746
32	19.634735322687536	29.441209045600207	26.713979646491698	24.210075985220556
33	17.586220983541075	19.88631721333055	24.705750183835214	37.82171161929316
34	20.37041660236217	19.95236171506904	31.412898423102416	28.26432325946638
35	15.759330204352128	24.60532168892359	24.73037502383049	34.90497308289379
36	19.876898495728668	21.80512559803183	27.689781485751634	30.628194420487866
37	13.679268835165633	26.729980118562363	38.90361587973092	20.687135166541086
38	17.87218686735722	21.189958512251142	29.563084981798042	31.37476963859359
39	20.01432098989587	19.679559340190462	39.25721496464009	21.048904705273575
40	15.761259339282635	19.566080814866595	28.08480023240402	36.58785961344675
41	21.730797163944697	22.407923524552213	30.183699036794277	25.677580274708816
42	18.016645030094505	17.704011692827248	33.204837816491604	31.07450546058664
43	28.034529245685547	18.169273646655107	23.648243806342087	30.14795330131726
44	19.341620291775985	20.096933356331647	32.964830735431626	27.59661561646074
45	16.892072844134976	15.513989632601927	31.018674026127297	36.5752634971358
46	27.675937105662126	21.970236852378054	28.560502210561673	21.793323831398144
47	12.537107477780904	18.202295897524355	44.05372527302933	25.20687135166541
48	19.11999673181847	23.252771145588408	29.259870362132673	28.36736176046045
49	17.431776710575292	15.081182537016696	40.42502246874801	27.06201828366
50	25.282901963632405	14.89825515419462	30.516985465670476	29.301857416502504
51	18.27730520276343	15.49322306246766	27.92990204533694	38.29956968943197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	243.5
2	421.0
3	2392.5
4	4364.0
5	3102.5
6	1841.0
7	1941.0
8	2041.0
9	2175.0
10	2309.0
11	2429.5
12	2550.0
13	2433.0
14	2316.0
15	2294.5
16	2273.0
17	2232.5
18	2192.0
19	2216.0
20	2240.0
21	2292.0
22	2344.0
23	2718.5
24	3093.0
25	3388.5
26	4154.0
27	4624.0
28	5548.0
29	6472.0
30	7329.5
31	8187.0
32	9565.5
33	10944.0
34	12741.5
35	14539.0
36	15859.0
37	17179.0
38	19761.0
39	22343.0
40	25622.0
41	28901.0
42	33377.0
43	37853.0
44	44804.0
45	51755.0
46	151308.5
47	250862.0
48	189558.0
49	128254.0
50	114957.0
51	101660.0
52	77053.0
53	52446.0
54	43901.0
55	35356.0
56	29957.0
57	24558.0
58	21345.0
59	18132.0
60	15740.0
61	13348.0
62	11249.5
63	9151.0
64	7205.5
65	5260.0
66	4297.0
67	3334.0
68	2587.0
69	1840.0
70	1506.5
71	1173.0
72	906.0
73	639.0
74	496.0
75	261.0
76	169.0
77	126.5
78	84.0
79	59.0
80	34.0
81	23.0
82	12.0
83	18.5
84	25.0
85	13.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	881224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.34043888443679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28416566974701	23.28098354254083
2	11.731258675956841	7.353255911426884
3	4.297462534737862	4.0405308578432635
4	2.073475166148672	2.5993448689231946
5	1.224640280079658	1.9190381926628042
6	0.8759022852765603	1.6470697224269133
7	0.6769047315643583	1.4850143959125202
8	0.5140808063209933	1.2889214473732062
9	0.4737430062726726	1.3362582361516242
>10	3.67880540404393	19.885801215837354
>50	0.09395612312811297	2.0100524040955827
>100	0.05982362439767295	3.743470943442801
>500	0.007707338112583625	1.6242603531220086
>1k	0.004404193207190643	3.5437884866040616
>5k	0.0011010483017976607	2.391703528253376
>10k+	0.002569112704194542	21.85050589338356
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	54874	6.227020598621917	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	43676	4.956288072045246	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	35217	3.996373226330649	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT	17542	1.9906402912312875	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	16948	1.9232340471889102	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA	10936	1.2410011529418172	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	10771	1.2222771962633792	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	8482	0.9625248517970459	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	7232	0.8206766951422114	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	5079	0.5763574301199241	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	4353	0.4939720207347962	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGT	3938	0.4468784327253911	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	3934	0.4464245186240956	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATG	3548	0.40262180784908264	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2501	0.28380979183499316	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTAT	2478	0.2811997857525442	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	2192	0.248744927509918	No Hit
TGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCTT	2048	0.23240401986328105	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATTCTCG	2010	0.2280918359009741	No Hit
CGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1668	0.18928218024021135	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCATTCTCGTCG	1094	0.12414550670431128	No Hit
TCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1045	0.11858505896344175	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	977	0.11086851924141876	No Hit
TGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	974	0.11052808366544715	No Hit
TTCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	968	0.10984721251350393	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	915	0.10383285067133896	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6487567292765517	0.0
2	0.0	0.0	0.0	3.710974735141122	0.0
3	0.0	0.0	0.0	4.518147485769793	0.0
4	0.0	0.0	0.0	6.896203462456764	0.0
5	0.0	0.0	0.0	12.774844988334408	0.0
6	0.0	0.0	0.0	13.83144353762494	0.0
7	0.0	0.0	0.0	15.481648252884623	0.0
8	0.0	0.0	0.0	17.643187203253657	0.0
9	0.0	0.0	0.0	18.14532967781177	0.0
10	0.0	0.0	0.0	24.52327671511443	0.0
11	0.0	0.0	0.0	26.63397728613837	0.0
12	0.0	0.0	0.0	35.02106161430011	0.0
13	0.0	0.0	0.0	36.032495710511746	0.0
14	0.0	0.0	0.0	36.51194248000508	0.0
15	0.0	0.0	0.0	38.20118380797618	0.0
16	0.0	0.0	0.0	39.35287736148811	0.0
17	0.0	0.0	0.0	40.536798816192025	0.0
18	0.0	0.0	0.0	41.61416393561682	0.0
19	0.0	0.0	0.0	44.69862373244487	0.0
20	0.0	0.0	0.0	45.734682668651786	0.0
21	0.0	0.0	0.0	46.862205296269735	0.0
22	0.0	0.0	0.0	48.11841257160495	0.0
23	0.0	0.0	0.0	49.39958512251142	0.0
24	0.0	0.0	0.0	50.3057111472225	0.0
25	0.0	0.0	0.0	51.08746470817862	0.0
26	0.0	0.0	0.0	51.822351638175995	0.0
27	0.0	0.0	0.0	52.544188537761116	0.0
28	0.0	0.0	0.0	53.28077764563834	0.0
29	0.0	0.0	0.0	54.007721078863035	0.0
30	0.0	0.0	0.0	54.740678873929895	0.0
31	0.0	0.0	0.0	55.44730965112162	0.0
32	0.0	0.0	0.0	56.14588345301535	0.0
33	0.0	0.0	0.0	56.831066788920864	0.0
34	0.0	0.0	0.0	57.48561092298893	0.0
35	0.0	0.0	0.0	58.1225658856318	0.0
36	0.0	0.0	0.0	58.7691665229272	0.0
37	0.0	0.0	0.0	59.38978057792343	0.0
38	0.0	0.0	0.0	60.03649469374415	0.0
39	0.0	0.0	0.0	60.78397774005247	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGA	25	3.8900616E-5	45.000004	4
CCGTTCG	25	3.8900616E-5	45.000004	1
GTACGAG	25	3.8900616E-5	45.000004	1
CTCACGT	20	7.03239E-4	45.0	13
GTTTACG	20	7.03239E-4	45.0	1
CGCGCCA	20	7.03239E-4	45.0	41
TTCGTCG	40	6.8121153E-9	45.0	1
GTTAGCG	20	7.03239E-4	45.0	1
CCTCACG	20	7.03239E-4	45.0	12
AACGCAG	40	6.8121153E-9	45.0	42
AGACACG	20	7.03239E-4	45.0	24
CAGTCGA	20	7.03239E-4	45.0	26
ACGAGCG	20	7.03239E-4	45.0	42
TTTTACG	45	3.8380676E-10	45.0	1
TACGGCT	6430	0.0	44.300156	7
ACGGCTG	6435	0.0	44.26573	8
GCGACTG	1465	0.0	43.924915	11
TCGGGAA	205	0.0	43.90244	5
GGCGACT	1490	0.0	43.640938	10
CGTTTTT	1815	0.0	43.512398	1
>>END_MODULE