SRR2933844.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933844.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	581256
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	39673	6.825391909932972	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	34332	5.906519674635616	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTC	28109	4.835907070206587	No Hit
GCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	13754	2.366255144032922	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGAGCGTT	12130	2.086860178647618	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	8895	1.5303067839299724	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTA	8376	1.4410173830463686	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTC	7225	1.242997921741883	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCT	6775	1.1655793660624578	No Hit
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	5350	0.9204206064109446	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	4849	0.8342279477545178	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	3957	0.6807671662744127	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGT	2872	0.49410242646957625	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATG	2732	0.47001665359153283	No Hit
TGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCTT	2259	0.3886411495107147	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTAT	1951	0.33565244917901926	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	1550	0.2666639140069092	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTT	1509	0.25961022337833933	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGAGCGT	1479	0.2544489863330443	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1425	0.24515875965151326	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	1005	0.17290144101738306	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	924	0.1589661009950865	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGCGTTCGTATGCCGTCTTC	899	0.15466507012400732	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	854	0.1469232145560648	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	834	0.14348238985920145	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCG	767	0.13195562712470923	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	732	0.1259341839051984	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	724	0.12455785402645306	No Hit
GCACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	598	0.102880658436214	No Hit
TGCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	593	0.10202045226199816	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	593	0.10202045226199816	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCC	20	7.0304045E-4	45.000004	11
CGGGTAT	20	7.0304045E-4	45.000004	6
GGATGCG	20	7.0304045E-4	45.000004	9
TAGCGGG	60	0.0	45.000004	3
TTCGTTA	20	7.0304045E-4	45.000004	22
CGATTCG	30	2.1636424E-6	45.000004	10
TACGGGA	30	2.1636424E-6	45.000004	4
TGCGGGT	60	0.0	45.000004	4
GCGTTGA	20	7.0304045E-4	45.000004	37
ATGTCAG	20	7.0304045E-4	45.000004	19
AATGCTC	65	0.0	45.000004	2
ACTAGCA	20	7.0304045E-4	45.000004	27
TGATTCG	30	2.1636424E-6	45.000004	26
TGCGACC	35	1.2105374E-7	45.000004	32
CCCCCGG	20	7.0304045E-4	45.000004	2
CATGTCA	20	7.0304045E-4	45.000004	18
CTCGAAC	30	2.1636424E-6	45.000004	13
TAAGGCG	30	2.1636424E-6	45.000004	29
GATTCAC	20	7.0304045E-4	45.000004	9
AACACTC	20	7.0304045E-4	45.000004	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content