##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933844.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	581256
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.65287412086929	28.0	16.0	31.0	16.0	33.0
2	25.441206628404696	28.0	16.0	31.0	16.0	34.0
3	26.515308229076346	30.0	16.0	33.0	16.0	34.0
4	31.03641940900395	33.0	28.0	35.0	19.0	37.0
5	33.250414619375974	35.0	32.0	35.0	28.0	37.0
6	31.513519000233977	35.0	28.0	36.0	17.0	37.0
7	33.17679645457423	35.0	32.0	35.0	28.0	37.0
8	31.96058535309743	35.0	31.0	37.0	17.0	37.0
9	34.20669205995293	35.0	32.0	39.0	28.0	39.0
10	33.7341498410339	35.0	32.0	37.0	27.0	39.0
11	34.424109170485984	35.0	32.0	39.0	27.0	39.0
12	34.581862380775426	37.0	34.0	39.0	27.0	39.0
13	34.78561941726193	37.0	34.0	39.0	30.0	39.0
14	34.71432380913057	37.0	33.0	39.0	27.0	40.0
15	35.25953280482266	37.0	33.0	39.0	27.0	40.0
16	34.64885867844805	36.0	32.0	39.0	27.0	40.0
17	34.25915947534305	36.0	32.0	38.0	27.0	40.0
18	34.506907455578954	36.0	32.0	39.0	27.0	40.0
19	34.22599336608999	36.0	32.0	39.0	27.0	40.0
20	34.278011753857164	36.0	32.0	39.0	27.0	40.0
21	34.213859297796496	37.0	32.0	39.0	26.0	40.0
22	35.45855870735098	37.0	34.0	39.0	30.0	40.0
23	35.81068754559093	37.0	34.0	39.0	30.0	40.0
24	36.10279807864349	38.0	35.0	40.0	30.0	40.0
25	33.88330270999353	36.0	32.0	39.0	24.0	40.0
26	34.99934452289525	37.0	33.0	39.0	27.0	40.0
27	35.15745041771612	37.0	33.0	39.0	29.0	40.0
28	35.25939861265948	37.0	34.0	39.0	27.0	40.0
29	35.600226062182585	38.0	34.0	40.0	29.0	40.0
30	33.418590087672214	36.0	31.0	39.0	23.0	40.0
31	34.278336911791015	36.0	32.0	39.0	25.0	40.0
32	34.78456480449234	37.0	33.0	39.0	27.0	40.0
33	35.38984371774227	37.0	34.0	39.0	30.0	40.0
34	35.405766134027004	38.0	34.0	40.0	29.0	40.0
35	35.63751427942249	38.0	35.0	40.0	30.0	40.0
36	35.55756843800322	38.0	34.0	40.0	29.0	41.0
37	35.66866922664024	38.0	35.0	40.0	30.0	41.0
38	35.64932663060682	38.0	35.0	40.0	30.0	41.0
39	35.4307310376151	38.0	34.0	40.0	29.0	40.0
40	35.19386466548303	37.0	33.0	40.0	28.0	40.0
41	34.46113416463658	37.0	33.0	39.0	26.0	40.0
42	35.494258983993284	38.0	35.0	40.0	30.0	40.0
43	35.37127530726565	38.0	34.0	40.0	30.0	40.0
44	36.09377795670066	38.0	35.0	40.0	31.0	41.0
45	36.44188103004528	39.0	35.0	40.0	32.0	41.0
46	36.182549513467386	38.0	35.0	40.0	31.0	41.0
47	35.58524987268949	38.0	34.0	40.0	27.0	41.0
48	35.88813362786793	38.0	35.0	40.0	30.0	41.0
49	36.29949970408908	38.0	35.0	40.0	31.0	41.0
50	35.79193677140537	38.0	35.0	40.0	28.0	41.0
51	35.55488287432732	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	8.0
11	8.0
12	13.0
13	12.0
14	30.0
15	37.0
16	101.0
17	203.0
18	558.0
19	1142.0
20	1628.0
21	2211.0
22	2498.0
23	2871.0
24	3440.0
25	4897.0
26	6460.0
27	8579.0
28	11802.0
29	16216.0
30	22199.0
31	30818.0
32	42209.0
33	56273.0
34	70398.0
35	85940.0
36	89629.0
37	77001.0
38	40216.0
39	3857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.720357295236525	3.4299860990682247	34.640846718141404	13.20880988755385
2	14.770772258694965	35.16952943281446	32.99578843057104	17.06390987791954
3	13.429538791857631	34.83181248881732	36.28212009854522	15.456528620779828
4	15.24801464414991	2.9255611985080585	63.05087603396782	18.77554812337421
5	37.41243101146483	3.6823705905831514	36.36556009744415	22.539638300507868
6	15.800439049231318	28.40056704791004	39.38023865560098	16.418755247257664
7	54.15341949158374	1.4265659193195426	38.34816328777681	6.0718513013199
8	48.196835817608765	23.628143193360586	16.11286593170651	12.062155057324139
9	44.486250464511336	4.253031366557936	16.951566951566953	34.30915121736378
10	37.814835459762996	18.707935918080846	24.79733542535475	18.67989319680141
11	31.054131054131055	19.34053153859917	29.02679714273917	20.578540264530602
12	21.02103032054723	14.732235022090096	33.426235600148644	30.820499057214036
13	21.904634102701735	20.511272141706925	42.98863151520156	14.595462240389775
14	19.189135251937184	29.753843401186398	24.810238517967985	26.246782828908433
15	10.49021429456212	23.502725133159917	45.29329589716063	20.713764675117332
16	11.01545618453831	25.493242220295357	24.583316129209848	38.90798546595648
17	12.806749521725367	28.05820499057214	35.81416793977181	23.32087754793069
18	11.797039514430818	24.648175674745723	33.95130544888999	29.603479361933466
19	12.755997357446633	28.27170816301251	28.81501438264723	30.157280096893622
20	15.28775616939868	25.93039899803185	43.82406375159999	14.957781080969488
21	19.25950011698804	34.775899087493286	27.44160920489423	18.52299159062444
22	13.368464153488308	22.056546513068252	34.48480531813865	30.09018401530479
23	16.147102137440303	33.393719806763286	28.09416160865436	22.36501644714205
24	21.97775162751008	22.23013611902501	23.60560579159613	32.18650646186878
25	11.91557592523776	40.00130751338481	20.609507686802374	27.473608874575056
26	15.123112707653771	25.534876199127403	29.719607195452607	29.622403897766215
27	21.12219056663501	33.31698941602323	20.840559065196747	24.720260952145008
28	15.177993861568742	28.928217515174037	32.81755371127352	23.076234911983704
29	20.720818365745902	30.64329658532557	22.068589399507275	26.56729564942125
30	21.610271549885077	35.81502814594602	24.77187332259796	17.802826981570945
31	28.59359731340408	24.5468433874231	17.552162902404447	29.307396396768375
32	27.50543650302105	39.53146290102812	16.920255446825493	16.04284514912534
33	23.858162324345898	20.444348101352933	21.361499924301857	34.33598964999931
34	27.12591353895702	28.043753526845315	20.565809213152207	24.26452372104546
35	22.79288299819701	28.79505759940543	21.672378435663457	26.73968096673411
36	25.723777474985205	29.384986993682645	25.349071665496787	19.542163865835363
37	17.038619816397592	34.493923503585336	29.87255185322818	18.594904826788884
38	36.077046946612164	22.48389694041868	19.17743644796785	22.26161966500131
39	22.17697537745847	29.668338907469344	25.598187373549692	22.556498341522495
40	32.80447857742544	20.92709580632286	25.32257731533094	20.945848300920762
41	20.216737547655423	23.66736859490483	25.877926421404684	30.237967436035067
42	27.454856379977155	18.4459171174147	32.56396493111469	21.535261571493454
43	25.457113560978296	20.35763931899198	29.06309784329108	25.122149276738647
44	21.63091649806626	20.37415527753692	30.54540512269981	27.449523101697014
45	23.130599942194145	15.078381986594547	26.687036348872095	35.10398172233921
46	30.971551261406333	25.252900615219453	25.705369062856988	18.070179060517226
47	16.22950988893018	17.533582449041386	45.63170100609714	20.605206655931294
48	21.9622679163742	22.465144445820776	25.00103224740906	30.57155539039597
49	21.56915369475756	14.04544641259617	41.22245619830161	23.16294369434466
50	25.71741194929601	13.990737299916045	32.2866344605475	28.00521629024044
51	20.441251359125758	14.621956590555623	26.80918562561075	38.12760642470787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	257.5
2	390.0
3	1469.0
4	2548.0
5	1807.5
6	1067.0
7	1246.5
8	1426.0
9	1598.5
10	1771.0
11	1975.0
12	2179.0
13	1999.5
14	1820.0
15	1752.0
16	1684.0
17	1591.5
18	1499.0
19	1529.0
20	1559.0
21	1612.0
22	1665.0
23	1878.5
24	2092.0
25	2243.0
26	3079.5
27	3765.0
28	4089.0
29	4413.0
30	4788.0
31	5163.0
32	5470.5
33	5778.0
34	6778.5
35	7779.0
36	8250.5
37	8722.0
38	9789.0
39	10856.0
40	12668.0
41	14480.0
42	16911.0
43	19342.0
44	23308.5
45	27275.0
46	105403.5
47	183532.0
48	137700.5
49	91869.0
50	82145.0
51	72421.0
52	52359.5
53	32298.0
54	26301.5
55	20305.0
56	16993.5
57	13682.0
58	12224.5
59	10767.0
60	9760.5
61	8754.0
62	7851.0
63	6948.0
64	5506.5
65	4065.0
66	3282.5
67	2500.0
68	2077.5
69	1655.0
70	1409.5
71	1164.0
72	907.0
73	650.0
74	554.5
75	347.0
76	235.0
77	168.0
78	101.0
79	64.5
80	28.0
81	22.0
82	16.0
83	13.0
84	10.0
85	7.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	581256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.79073052986392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.07885757009839	18.589663924908496
2	11.49940968578207	5.9315635291702495
3	4.456727243180308	3.448267541218998
4	2.351545459338217	2.4259230108206813
5	1.4953961707240866	1.9283679837267649
6	1.0636935878785283	1.6460060814791546
7	0.843319150639963	1.5224871875380301
8	0.6889145768443593	1.421408816759048
9	0.6183394536330469	1.4352683602169876
>10	4.677070060572029	19.999405451576173
>50	0.11673743495971382	2.1409346438910735
>100	0.08569742335192865	4.628887957910484
>500	0.010121742915582124	1.7798194279852064
>1k	0.0074226114714268905	4.453116214264736
>5k	0.0033739143051940415	6.3732049743078365
>10k+	0.0033739143051940415	22.275674894226114
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	39673	6.825391909932972	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	34332	5.906519674635616	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTC	28109	4.835907070206587	No Hit
GCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	13754	2.366255144032922	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGAGCGTT	12130	2.086860178647618	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	8895	1.5303067839299724	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTA	8376	1.4410173830463686	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTC	7225	1.242997921741883	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCT	6775	1.1655793660624578	No Hit
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	5350	0.9204206064109446	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	4849	0.8342279477545178	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	3957	0.6807671662744127	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGT	2872	0.49410242646957625	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATG	2732	0.47001665359153283	No Hit
TGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCTT	2259	0.3886411495107147	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTAT	1951	0.33565244917901926	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	1550	0.2666639140069092	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTT	1509	0.25961022337833933	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGAGCGT	1479	0.2544489863330443	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1425	0.24515875965151326	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	1005	0.17290144101738306	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	924	0.1589661009950865	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGCGTTCGTATGCCGTCTTC	899	0.15466507012400732	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	854	0.1469232145560648	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	834	0.14348238985920145	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCG	767	0.13195562712470923	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	732	0.1259341839051984	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	724	0.12455785402645306	No Hit
GCACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	598	0.102880658436214	No Hit
TGCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	593	0.10202045226199816	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	593	0.10202045226199816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9672158222882861	0.0
2	0.0	0.0	0.0	5.242612549375834	0.0
3	0.0	0.0	0.0	6.430213193498218	0.0
4	0.0	0.0	0.0	9.617449110202733	0.0
5	0.0	0.0	0.0	17.121887774061687	0.0
6	0.0	0.0	0.0	18.432325859862093	0.0
7	0.0	0.0	0.0	20.67230273752013	0.0
8	0.0	0.0	0.0	23.373006042088168	0.0
9	0.0	0.0	0.0	23.987537332947962	0.0
10	0.0	0.0	0.0	31.528448738593667	0.0
11	0.0	0.0	0.0	33.78408136862243	0.0
12	0.0	0.0	0.0	43.177016667354835	0.0
13	0.0	0.0	0.0	44.282037518752496	0.0
14	0.0	0.0	0.0	44.85132196484854	0.0
15	0.0	0.0	0.0	46.79590404228085	0.0
16	0.0	0.0	0.0	48.00827862422065	0.0
17	0.0	0.0	0.0	49.12379399094375	0.0
18	0.0	0.0	0.0	50.065031586770715	0.0
19	0.0	0.0	0.0	53.26981570942924	0.0
20	0.0	0.0	0.0	54.252515242853406	0.0
21	0.0	0.0	0.0	55.12786104573544	0.0
22	0.0	0.0	0.0	56.09628115666763	0.0
23	0.0	0.0	0.0	56.91433722834689	0.0
24	0.0	0.0	0.0	57.58030884842479	0.0
25	0.0	0.0	0.0	58.16817374788389	0.0
26	0.0	0.0	0.0	58.716469163329066	0.0
27	0.0	0.0	0.0	59.26029150666832	0.0
28	0.0	0.0	0.0	59.72893183038111	0.0
29	0.0	0.0	0.0	60.26673273050085	0.0
30	0.0	0.0	0.0	60.823458166453335	0.0
31	0.0	0.0	0.0	61.35489354088388	0.0
32	0.0	0.0	0.0	61.83299613251304	0.0
33	0.0	0.0	0.0	62.28047538434012	0.0
34	0.0	0.0	0.0	62.7152235847888	0.0
35	0.0	0.0	0.0	63.18506819714549	0.0
36	0.0	0.0	0.0	63.65680526308546	0.0
37	0.0	0.0	0.0	64.12338109198012	0.0
38	0.0	0.0	0.0	64.59615040532915	0.0
39	0.0	0.0	0.0	65.06444664657225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCC	20	7.0304045E-4	45.000004	11
CGGGTAT	20	7.0304045E-4	45.000004	6
GGATGCG	20	7.0304045E-4	45.000004	9
TAGCGGG	60	0.0	45.000004	3
TTCGTTA	20	7.0304045E-4	45.000004	22
CGATTCG	30	2.1636424E-6	45.000004	10
TACGGGA	30	2.1636424E-6	45.000004	4
TGCGGGT	60	0.0	45.000004	4
GCGTTGA	20	7.0304045E-4	45.000004	37
ATGTCAG	20	7.0304045E-4	45.000004	19
AATGCTC	65	0.0	45.000004	2
ACTAGCA	20	7.0304045E-4	45.000004	27
TGATTCG	30	2.1636424E-6	45.000004	26
TGCGACC	35	1.2105374E-7	45.000004	32
CCCCCGG	20	7.0304045E-4	45.000004	2
CATGTCA	20	7.0304045E-4	45.000004	18
CTCGAAC	30	2.1636424E-6	45.000004	13
TAAGGCG	30	2.1636424E-6	45.000004	29
GATTCAC	20	7.0304045E-4	45.000004	9
AACACTC	20	7.0304045E-4	45.000004	30
>>END_MODULE