SRR2933843.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933843.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890640
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	64096	7.196622653372856	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	54428	6.111111111111111	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	41971	4.712453965687596	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT	19843	2.2279484415701067	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	18728	2.1027575675918437	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA	12831	1.4406494206413367	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	11598	1.302209646995419	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC	10188	1.1438965238480194	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	9326	1.0471121889876942	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	5516	0.6193299200574868	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	4667	0.5240052097368185	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGT	4593	0.5156965777418486	No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	4459	0.5006512171023085	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATG	4019	0.45124854037546036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT	2958	0.3321207221773107	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTAT	2948	0.3309979340698823	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2623	0.29450732057846046	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGTTCAC	2347	0.2635183688134375	No Hit
TGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCTT	1932	0.21692266235516033	TruSeq Adapter, Index 19 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1885	0.211645558250247	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTTCACTCGTATGCCGTCTTC	1627	0.18267762507859517	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGAGTTCACTCG	1205	0.13529596694511814	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	1165	0.13080481451540465	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	1110	0.12462947992454865	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	1092	0.12260846133117759	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1091	0.12249618252043475	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1047	0.11755591484774992	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1041	0.11688224198329292	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	7.032429E-4	45.000004	16
CGAAATT	20	7.032429E-4	45.000004	20
ACCATCG	20	7.032429E-4	45.000004	14
GTTAGCG	40	6.8121153E-9	45.000004	1
TCGGATC	20	7.032429E-4	45.000004	24
AGTGCGA	20	7.032429E-4	45.000004	16
CTAACGG	20	7.032429E-4	45.000004	2
TACGCCA	20	7.032429E-4	45.000004	40
CGCTCGA	20	7.032429E-4	45.000004	41
CGAGGGA	40	6.8121153E-9	45.000004	4
ACGGACG	20	7.032429E-4	45.000004	43
ACTACCG	20	7.032429E-4	45.000004	14
CTACCGA	20	7.032429E-4	45.000004	15
GTGACGG	20	7.032429E-4	45.000004	2
GTGCGAA	20	7.032429E-4	45.000004	17
TTCTCCG	160	0.0	45.000004	1
TCTTCGC	25	3.890096E-5	45.0	1
ATTCGGC	45	3.8380676E-10	45.0	28
CGCGAAC	25	3.890096E-5	45.0	32
GCCGATT	95	0.0	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content