##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933843.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890640
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.635862974939368	28.0	16.0	31.0	16.0	33.0
2	25.381119195185484	28.0	16.0	31.0	16.0	34.0
3	26.461187460702416	30.0	16.0	33.0	16.0	34.0
4	30.975051648252943	33.0	28.0	35.0	19.0	37.0
5	33.22879502380311	35.0	32.0	35.0	28.0	37.0
6	31.3465238480194	35.0	28.0	36.0	17.0	37.0
7	33.1542632264439	35.0	32.0	35.0	28.0	37.0
8	31.896827000808408	35.0	30.0	37.0	17.0	37.0
9	34.16877189436809	35.0	32.0	38.0	28.0	39.0
10	33.624128716428636	35.0	32.0	37.0	27.0	39.0
11	34.45150229048774	35.0	32.0	39.0	27.0	39.0
12	34.648708793676455	37.0	34.0	39.0	27.0	39.0
13	34.79420753615378	35.0	34.0	38.0	30.0	39.0
14	34.64057194826192	37.0	33.0	39.0	27.0	40.0
15	35.05673560585646	37.0	33.0	39.0	27.0	40.0
16	34.737331581783884	36.0	32.0	39.0	27.0	40.0
17	34.11550570376359	36.0	32.0	38.0	27.0	40.0
18	34.48229363154586	36.0	32.0	39.0	27.0	40.0
19	34.24725366028923	36.0	32.0	39.0	27.0	40.0
20	33.92060428455942	36.0	32.0	39.0	25.0	40.0
21	33.90151800952124	36.0	32.0	39.0	25.0	40.0
22	35.26129300278451	37.0	34.0	39.0	29.0	40.0
23	35.4628929758376	37.0	34.0	39.0	29.0	40.0
24	35.748761564717505	38.0	34.0	39.0	30.0	40.0
25	33.492487424773195	36.0	31.0	39.0	24.0	40.0
26	34.77005411838678	37.0	33.0	39.0	27.0	40.0
27	34.81397646636127	37.0	33.0	39.0	27.0	40.0
28	34.94445454953741	37.0	33.0	39.0	27.0	40.0
29	35.060428455941796	37.0	33.0	40.0	25.0	40.0
30	32.76868768526004	36.0	30.0	39.0	20.0	40.0
31	33.93794462409054	36.0	31.0	39.0	25.0	40.0
32	34.36270097907123	37.0	32.0	39.0	25.0	40.0
33	34.950446869666756	37.0	33.0	39.0	27.0	40.0
34	35.06266841821611	37.0	34.0	39.0	27.0	40.0
35	35.03158627503817	38.0	34.0	40.0	25.0	40.0
36	35.30163927063685	38.0	34.0	40.0	27.0	41.0
37	34.73417205604958	38.0	33.0	40.0	24.0	41.0
38	34.946864052815954	37.0	33.0	40.0	27.0	40.0
39	34.376715620228154	37.0	33.0	39.0	24.0	40.0
40	34.22717371777598	37.0	33.0	39.0	24.0	40.0
41	33.976897511901555	36.0	33.0	39.0	24.0	40.0
42	34.78610661995868	37.0	33.0	39.0	27.0	40.0
43	35.215311461421	38.0	34.0	40.0	28.0	40.0
44	35.791519581424595	38.0	35.0	40.0	30.0	41.0
45	36.16784447139136	38.0	35.0	40.0	31.0	41.0
46	35.637772837510106	38.0	35.0	40.0	28.0	41.0
47	35.06944444444444	38.0	34.0	40.0	26.0	41.0
48	35.36066760980868	38.0	34.0	40.0	28.0	41.0
49	35.733159301176684	38.0	35.0	40.0	29.0	41.0
50	35.40263406090003	38.0	34.0	40.0	28.0	41.0
51	35.01332524925896	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	9.0
12	10.0
13	13.0
14	25.0
15	70.0
16	166.0
17	551.0
18	1444.0
19	2951.0
20	4240.0
21	4999.0
22	5216.0
23	5548.0
24	6717.0
25	8894.0
26	12186.0
27	15545.0
28	20248.0
29	27440.0
30	36688.0
31	49969.0
32	66534.0
33	86559.0
34	106735.0
35	125063.0
36	127189.0
37	111944.0
38	58495.0
39	5181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.055173807599026	2.868274499236504	38.26203628851163	9.814515404652834
2	13.495576214856733	35.97907122967754	34.63711488367915	15.888237671786582
3	11.867758016707088	35.90979520344921	38.3534312404563	13.869015539387409
4	13.499393694421988	2.639225725321117	67.20886104374382	16.65251953651307
5	38.52780023353993	3.0822779125123505	37.91610527261295	20.47381658133477
6	14.421764124674391	29.51315907661906	40.56846761879098	15.496609179915566
7	56.332749483517475	0.7801131770412288	37.96112907572083	4.926008263720471
8	51.92165184586365	24.94150273960298	13.626156471750651	9.510688942782718
9	49.545495374112996	2.942939908380491	13.287635857361	34.22392886014551
10	38.492320129345195	24.7475972334501	20.088363424054613	16.671719213150094
11	30.72240186831941	19.82709063145603	29.782403664780382	19.668103835444175
12	20.81065301356328	15.556678343662982	31.91581334770502	31.71685529506871
13	22.90251953651307	20.207603521063504	43.01446151082368	13.87541543159975
14	20.199407167879276	30.553983652205158	23.299761968921224	25.94684721099434
15	10.427782268930208	22.817636755591487	44.20899577831672	22.545585197161593
16	11.604015090272163	26.148949070331444	22.999079313751906	39.247956525644476
17	14.076731339261656	28.980957513698012	35.15539387406809	21.786917272972246
18	12.12555016617264	24.641606036108865	32.900273960298215	30.33256983742028
19	12.54435013024342	28.960859606575045	28.10900026946915	30.385789993712386
20	15.248809844606125	26.968584388754152	43.010082637204704	14.772523129435013
21	18.613693523758197	36.69204167789455	27.303512081200036	17.39075271714722
22	13.791879996407078	21.813190514686067	35.09352824934878	29.30140123955807
23	16.25909458367017	34.509229318243065	27.93968382286895	21.291992275217822
24	20.944377077158	23.89046079223929	23.955245666037904	31.209916464564806
25	12.52964160603611	42.249618252043476	19.926345100152698	25.29439504176772
26	17.98448306835534	22.434092338093954	30.04783077337645	29.533593820174257
27	21.30108685888799	31.765135183688137	21.759072127908023	25.174705829515855
28	15.894974400431149	26.30670079942513	34.89995957962813	22.898365220515586
29	26.28997125662445	18.266190604509116	27.518076888529595	27.92576125033684
30	16.663410581155127	36.127391538668824	30.137878379592202	17.07131950058385
31	28.153799514955537	21.165117219078418	20.938650857810114	29.742432408155935
32	23.23621216204078	34.465216024431875	25.12069972154855	17.177872091978802
33	15.920349411659032	20.336611874607023	24.99281415611246	38.750224557621486
34	29.991466810383542	21.546640617982575	22.407257702326415	26.054634869307463
35	15.304724692356059	32.21862930027845	22.73679601185664	29.73984999550885
36	31.157594538758644	22.009678433486034	20.045697475972336	26.787029551782986
37	14.558744273780652	29.22527620587443	36.06608730800323	20.149892212341687
38	25.20434743555196	22.568939189796104	27.51818916734034	24.708524207311598
39	19.727723883948624	21.055533099793408	33.87047516392707	25.346267852330907
40	15.944377077157998	27.001369801491066	35.259251774005214	21.79500134734573
41	18.15233989041588	24.17385251055421	19.370115871732686	38.30369172729722
42	16.676098086769066	26.74750741040151	32.66370250606306	23.91269199676637
43	24.27804724692356	19.050682655169314	21.99766460073655	34.67360549717057
44	20.361313212970447	20.71443007275667	31.138507140932365	27.785749573340517
45	20.366478038264617	17.41365759453876	26.97397377166981	35.24589059552682
46	30.058946375639987	24.88727207401419	25.256220246115152	19.797561304230666
47	12.351118296954999	18.96411569208659	47.40130692535705	21.28345908560137
48	20.774499236504088	24.696285816940627	24.105137878379594	30.42407706817569
49	17.692894996856193	18.467618790981767	40.06096739423336	23.77851881792868
50	24.12590945836702	15.693658492769243	29.390438336477143	30.789993712386597
51	19.795989400880266	15.664466001976107	26.23652654271086	38.30301805443277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	144.0
2	221.0
3	1859.5
4	3498.0
5	2399.0
6	1300.0
7	1472.5
8	1645.0
9	1924.5
10	2204.0
11	2539.5
12	2875.0
13	3045.0
14	3215.0
15	3116.0
16	3017.0
17	2827.0
18	2637.0
19	2540.0
20	2443.0
21	2441.0
22	2439.0
23	2506.5
24	2574.0
25	2625.0
26	3469.5
27	4263.0
28	4749.0
29	5235.0
30	5871.0
31	6507.0
32	8334.0
33	10161.0
34	12804.0
35	15447.0
36	16839.0
37	18231.0
38	20325.5
39	22420.0
40	25306.0
41	28192.0
42	32727.5
43	37263.0
44	44623.0
45	51983.0
46	169852.5
47	287722.0
48	213420.5
49	139119.0
50	120816.5
51	102514.0
52	75237.0
53	47960.0
54	39098.0
55	30236.0
56	25112.5
57	19989.0
58	16581.5
59	13174.0
60	10706.5
61	8239.0
62	6595.5
63	4952.0
64	3845.5
65	2739.0
66	2135.5
67	1532.0
68	1224.5
69	917.0
70	689.0
71	461.0
72	389.5
73	318.0
74	208.0
75	104.0
76	110.0
77	69.0
78	28.0
79	21.5
80	15.0
81	8.0
82	1.0
83	1.5
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	890640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.2948213862181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56829150833344	21.098964893037387
2	11.616004030349625	6.573455185206643
3	4.284500212557974	3.6368750473062406
4	2.155077772566332	2.439101625926925
5	1.2063653209428145	1.7066945641302307
6	0.8258811981441119	1.4020896592634065
7	0.6379657192286688	1.2635788253273745
8	0.5065445342257239	1.1466069696065517
9	0.4074734123402434	1.0376448679619987
>10	3.578914202905109	18.51138985118334
>50	0.11836811724152936	2.3127739631572313
>100	0.07448333900179527	4.228769688532556
>500	0.008857478151564843	1.5676328677540072
>1k	0.007247027578553054	4.762819749140854
>5k	8.052252865058948E-4	1.6907787968319872
>10k+	0.003220901146023579	26.62082344563329
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	64096	7.196622653372856	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	54428	6.111111111111111	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	41971	4.712453965687596	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT	19843	2.2279484415701067	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	18728	2.1027575675918437	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA	12831	1.4406494206413367	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	11598	1.302209646995419	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC	10188	1.1438965238480194	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	9326	1.0471121889876942	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	5516	0.6193299200574868	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	4667	0.5240052097368185	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGT	4593	0.5156965777418486	No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	4459	0.5006512171023085	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATG	4019	0.45124854037546036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT	2958	0.3321207221773107	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTAT	2948	0.3309979340698823	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2623	0.29450732057846046	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGTTCAC	2347	0.2635183688134375	No Hit
TGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCTT	1932	0.21692266235516033	TruSeq Adapter, Index 19 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1885	0.211645558250247	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTTCACTCGTATGCCGTCTTC	1627	0.18267762507859517	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGAGTTCACTCG	1205	0.13529596694511814	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	1165	0.13080481451540465	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	1110	0.12462947992454865	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	1092	0.12260846133117759	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1091	0.12249618252043475	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1047	0.11755591484774992	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1041	0.11688224198329292	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.122788107428366E-4	0.0	0.0	0.6866972065031888	0.0
2	1.122788107428366E-4	0.0	0.0	4.098401149735022	0.0
3	1.122788107428366E-4	0.0	0.0	4.955425312135094	0.0
4	1.122788107428366E-4	0.0	0.0	7.44498338273601	0.0
5	1.122788107428366E-4	0.0	0.0	14.279843707895447	0.0
6	1.122788107428366E-4	0.0	0.0	15.41161412018324	0.0
7	1.122788107428366E-4	0.0	0.0	17.359314650139225	0.0
8	1.122788107428366E-4	0.0	0.0	19.783751010509295	0.0
9	1.122788107428366E-4	0.0	0.0	20.332233000988055	0.0
10	1.122788107428366E-4	0.0	0.0	28.018391269199675	0.0
11	1.122788107428366E-4	0.0	0.0	30.17257253211174	0.0
12	1.122788107428366E-4	0.0	0.0	40.000898230485944	0.0
13	2.245576214856732E-4	0.0	0.0	41.04632623731249	0.0
14	3.3683643222850985E-4	0.0	0.0	41.574485763046795	0.0
15	3.3683643222850985E-4	0.0	0.0	43.474355519626336	0.0
16	3.3683643222850985E-4	0.0	0.0	44.5853543519267	0.0
17	3.3683643222850985E-4	0.0	0.0	45.61820713195006	0.0
18	4.491152429713464E-4	0.0	0.0	46.518907751729095	0.0
19	4.491152429713464E-4	0.0	0.0	49.783975568130785	0.0
20	4.491152429713464E-4	0.0	0.0	50.78325698374203	0.0
21	4.491152429713464E-4	0.0	0.0	51.75278451450642	0.0
22	4.491152429713464E-4	0.0	0.0	52.95978172999192	0.0
23	4.491152429713464E-4	0.0	0.0	53.98926614569299	0.0
24	4.491152429713464E-4	0.0	0.0	54.80463486930746	0.0
25	4.491152429713464E-4	0.0	0.0	55.48897422078505	0.0
26	4.491152429713464E-4	0.0	0.0	56.16073834545944	0.0
27	4.491152429713464E-4	0.0	0.0	56.83923919877841	0.0
28	4.491152429713464E-4	0.0	0.0	57.43364322285098	0.0
29	4.491152429713464E-4	0.0	0.0	58.06914129165544	0.0
30	4.491152429713464E-4	0.0	0.0	58.79940267672685	0.0
31	4.491152429713464E-4	0.0	0.0	59.39942064133657	0.0
32	4.491152429713464E-4	0.0	0.0	59.92308901464116	0.0
33	4.491152429713464E-4	0.0	0.0	60.48055330997934	0.0
34	4.491152429713464E-4	0.0	0.0	61.058115512440494	0.0
35	4.491152429713464E-4	0.0	0.0	61.66745261834187	0.0
36	4.491152429713464E-4	0.0	0.0	62.238165813347706	0.0
37	4.491152429713464E-4	0.0	0.0	62.75891493757298	0.0
38	4.491152429713464E-4	0.0	0.0	63.33075092068625	0.0
39	4.491152429713464E-4	0.0	0.0	63.932789903889336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	7.032429E-4	45.000004	16
CGAAATT	20	7.032429E-4	45.000004	20
ACCATCG	20	7.032429E-4	45.000004	14
GTTAGCG	40	6.8121153E-9	45.000004	1
TCGGATC	20	7.032429E-4	45.000004	24
AGTGCGA	20	7.032429E-4	45.000004	16
CTAACGG	20	7.032429E-4	45.000004	2
TACGCCA	20	7.032429E-4	45.000004	40
CGCTCGA	20	7.032429E-4	45.000004	41
CGAGGGA	40	6.8121153E-9	45.000004	4
ACGGACG	20	7.032429E-4	45.000004	43
ACTACCG	20	7.032429E-4	45.000004	14
CTACCGA	20	7.032429E-4	45.000004	15
GTGACGG	20	7.032429E-4	45.000004	2
GTGCGAA	20	7.032429E-4	45.000004	17
TTCTCCG	160	0.0	45.000004	1
TCTTCGC	25	3.890096E-5	45.0	1
ATTCGGC	45	3.8380676E-10	45.0	28
CGCGAAC	25	3.890096E-5	45.0	32
GCCGATT	95	0.0	45.0	9
>>END_MODULE