FastQCFastQC Report
Sat 14 Jan 2017
SRR2933841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014591
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC553085.451260655771636No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG452924.464064830064529No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC349423.4439493352493766No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATAACCCT165271.6289322495468617No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC143231.4117018581871907TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTA108621.0705791791963462No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT104231.0273105123148145No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC86380.8513775501655347No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT77610.7649387782860286No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT45690.4503292459720222TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC38670.38113880371499453TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATAACCCTCGT38080.37532365258513034No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34970.3446709067988973No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATG33660.33175930005292775No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC31310.30859725741702815TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTAT24470.2411809290640268No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT20880.20579721286705677No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATAACCC20350.20057343303853475No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG20090.19801082406605222No Hit
TGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCTT17800.17544015273149477TruSeq Adapter, Index 13 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC16260.16026162266371374No Hit
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG14080.13877513204828348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA207.0328964E-445.025
CGCGAAC207.0328964E-445.032
CCCCGTT207.0328964E-445.016
TGCGATC207.0328964E-445.032
ATTTCGG253.8904815E-545.02
TACCGGG700.045.03
GATACGC600.044.9999965
GCGCGAC302.1652486E-644.9999969
TACGGCT65900.044.0098657
ACCGGTG1950.043.84615317
ACGGCTG66350.043.6774678
GATACCT47300.043.4778025
TGATACC47550.043.0599334
GGCGACT12300.042.8048810
CGGCTGT69150.042.6247339
AGGCACG900.042.510
GCGACTG12300.042.25609611
CGTTTGG5600.042.18752
CGTTTTT19100.041.9371721
AAGGCGA700.041.78571330
Produced by FastQC (version 0.11.5)