##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933841.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1014591
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.34978134046133	28.0	16.0	31.0	16.0	34.0
2	26.412721973682007	30.0	16.0	33.0	16.0	34.0
3	27.472273063727158	31.0	25.0	33.0	16.0	34.0
4	31.53929514454593	35.0	28.0	35.0	19.0	37.0
5	33.35924426690164	35.0	32.0	35.0	28.0	37.0
6	31.84327970581249	35.0	30.0	37.0	17.0	37.0
7	33.14688381820852	35.0	32.0	35.0	28.0	37.0
8	32.44817665443514	35.0	32.0	37.0	25.0	37.0
9	34.247925518755835	35.0	32.0	38.0	28.0	39.0
10	32.89276072821462	34.0	31.0	37.0	27.0	39.0
11	33.243049662376265	35.0	32.0	37.0	26.0	39.0
12	33.42147722579838	35.0	31.0	38.0	24.0	39.0
13	33.45707777813917	35.0	32.0	37.0	25.0	39.0
14	33.728269815127476	36.0	32.0	38.0	23.0	40.0
15	34.26783699047202	36.0	32.0	39.0	25.0	40.0
16	34.16241618543827	36.0	32.0	38.0	27.0	40.0
17	33.636484061065	35.0	32.0	38.0	25.0	40.0
18	34.07539885530228	36.0	32.0	38.0	27.0	40.0
19	33.863135982873885	35.0	32.0	39.0	25.0	40.0
20	33.65748956968867	35.0	31.0	38.0	25.0	40.0
21	33.26623733110189	35.0	31.0	39.0	23.0	40.0
22	34.343843972595856	36.0	32.0	39.0	26.0	40.0
23	35.05986254559719	37.0	33.0	39.0	29.0	40.0
24	35.323262280071475	37.0	34.0	39.0	29.0	40.0
25	33.522631286892945	35.0	32.0	39.0	24.0	40.0
26	34.240990704628764	36.0	32.0	39.0	27.0	40.0
27	34.505391827839986	36.0	33.0	39.0	27.0	40.0
28	34.59096325514419	36.0	33.0	39.0	27.0	40.0
29	35.02944733395033	37.0	34.0	39.0	27.0	40.0
30	32.95169580648754	35.0	31.0	38.0	23.0	40.0
31	33.79147557981492	35.0	31.0	39.0	25.0	40.0
32	33.9176623880953	36.0	32.0	38.0	25.0	40.0
33	34.484690875436506	36.0	33.0	39.0	26.0	40.0
34	34.70415862155292	37.0	34.0	39.0	25.0	40.0
35	34.77683815448787	37.0	34.0	40.0	26.0	40.0
36	34.94053564441238	37.0	34.0	40.0	27.0	40.0
37	35.05881680401265	37.0	34.0	40.0	27.0	41.0
38	34.87137674195809	37.0	34.0	40.0	27.0	40.0
39	34.916130736424826	37.0	34.0	40.0	28.0	40.0
40	34.314044772721225	36.0	33.0	39.0	25.0	40.0
41	33.69339369263082	35.0	32.0	39.0	23.0	40.0
42	34.753983624928665	37.0	33.0	39.0	27.0	40.0
43	35.07248536602434	37.0	34.0	40.0	29.0	40.0
44	35.60016499259307	38.0	35.0	40.0	30.0	40.0
45	35.90891206407311	38.0	35.0	40.0	31.0	41.0
46	35.67228075155408	38.0	35.0	40.0	30.0	41.0
47	35.31496238385714	37.0	34.0	40.0	29.0	41.0
48	35.47270772163365	37.0	34.0	40.0	30.0	41.0
49	35.780472131134616	38.0	35.0	40.0	31.0	41.0
50	35.512501096500955	37.0	34.0	40.0	29.0	41.0
51	35.02762985281754	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	17.0
11	19.0
12	26.0
13	25.0
14	62.0
15	83.0
16	202.0
17	451.0
18	1117.0
19	2424.0
20	4068.0
21	5441.0
22	6283.0
23	6880.0
24	7971.0
25	10798.0
26	14585.0
27	19737.0
28	25654.0
29	34768.0
30	47134.0
31	64483.0
32	86705.0
33	105770.0
34	114886.0
35	130561.0
36	134536.0
37	118812.0
38	64463.0
39	6617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82968112273813	3.391415851313485	39.98103669360363	12.797866332344757
2	17.072199536562024	27.71698152260369	37.717267352066	17.493551588768284
3	16.297108884269623	27.95993656557174	39.27819190195853	16.46476264820011
4	16.454709336077297	3.2817164749145222	60.49994529815462	19.763628890853553
5	34.155142318431764	4.13467101521697	39.44584566588901	22.264341000462256
6	17.619217990303483	24.09286106421208	42.73741832915924	15.550502616325199
7	62.47975785316447	1.6525871016005464	31.041276731214847	4.826378314020132
8	59.68602126374076	19.683399517638144	12.462065995065991	8.168513223555108
9	55.90528597237705	3.5431025901077375	13.016772275724897	27.53483916179032
10	47.17585706949894	16.950475610369104	20.038616545977643	15.835050774154313
11	39.23837290100149	17.28952848980525	25.66393748811097	17.80816112108229
12	30.05792481896646	13.56793032857575	29.12099555387343	27.253149298584354
13	28.640210685882288	16.419029934229656	38.88325443454554	16.057504945342508
14	21.226878614141068	24.569210647443157	30.38396752977308	23.819943208642695
15	15.174489030555169	19.105629756226893	45.24059448585686	20.479286727361075
16	22.305145620254862	21.397292110811154	23.756272231864862	32.541290037069125
17	21.12723254986492	25.27580079066343	33.48472438647691	20.112242272994735
18	20.9851063137757	21.593331697206068	31.69109522950627	25.730466759511962
19	19.81074147119381	26.78448754227073	27.155770157630023	26.249000828905437
20	19.926551684373308	28.708218385536632	37.679419588780114	13.685810341309946
21	21.549767344673864	33.180463851936395	29.054761968123117	16.215006835266625
22	16.02566945695359	19.597650678943534	39.049922579640466	25.32675728446241
23	19.8667246210542	28.512375922908838	32.10209828393904	19.51880117209792
24	24.07482423952115	23.046725232137877	24.65702928569246	28.221421242648514
25	18.459950857044856	33.64813998941445	21.28857835324776	26.603330800292923
26	15.791584983505668	19.795661503009587	31.215829826994323	33.19692368649042
27	19.759883539278388	27.026752652053883	26.952338429968332	26.261025378699397
28	12.005724474196992	26.110422820624272	33.898191487998616	27.985661217180123
29	20.766101808512	25.062611436529597	24.125485047669454	30.04580170728895
30	19.135099759410444	33.020497914923354	28.9204221208349	18.923980204831306
31	22.7212738926326	20.831054089776078	27.711166371473826	28.7365056461175
32	26.159506638635666	32.99763155793812	19.604944258326753	21.237917545099457
33	20.812228770016684	23.98345737346379	21.491517271491663	33.71279658502786
34	20.630776342388213	20.746685117451268	27.059278073627695	31.56326046653282
35	14.734804468007306	30.431868605181794	22.22540905645723	32.60791787035367
36	16.82530201825169	25.906301159777684	24.288703526839882	32.979693295130744
37	15.545278836496676	34.37503388064747	27.73748239438355	22.3422048884723
38	19.46232521281975	28.54302866869507	25.973224678712896	26.021421439772286
39	19.401808216315736	29.327581261808945	22.72738472941313	28.543225792462184
40	18.19067979116708	26.641671372996605	24.72395280462768	30.443696031208635
41	16.44505027148871	22.490441961342057	20.814692817105612	40.24981495006362
42	15.40926343718799	18.927331308872244	31.642799906563333	34.02060534737643
43	19.054574700544357	18.87992304288132	24.000607141202714	38.06489511537161
44	20.302072460725554	20.413841636679216	29.484294656664606	29.799791245930624
45	17.67224428365716	16.421592543202138	28.409871564009535	37.49629160913116
46	25.423840739766074	25.160779072552387	24.426098792518363	24.989281395163175
47	11.812444620541676	21.908828286472083	42.105439531791625	24.17328756119461
48	18.578126555429726	25.86667928258776	25.78004338694114	29.77515077504137
49	19.36760724272145	16.943182030985884	36.99027489894943	26.698935827343234
50	21.539516908783934	18.108282056513413	27.451652932068193	32.90054810263446
51	17.74626425820848	18.41658362827977	25.903541427038085	37.93361068647366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	442.5
2	739.0
3	3490.0
4	6241.0
5	4130.5
6	2020.0
7	1961.0
8	1902.0
9	2000.0
10	2098.0
11	2156.5
12	2215.0
13	2144.0
14	2073.0
15	1995.0
16	1917.0
17	1904.0
18	1891.0
19	2005.5
20	2120.0
21	2253.5
22	2387.0
23	2731.0
24	3075.0
25	3250.5
26	4223.0
27	5020.0
28	6020.5
29	7021.0
30	8194.0
31	9367.0
32	10681.0
33	11995.0
34	13772.5
35	15550.0
36	16637.0
37	17724.0
38	18981.5
39	20239.0
40	22858.5
41	25478.0
42	29009.5
43	32541.0
44	39879.0
45	47217.0
46	147138.5
47	247060.0
48	187461.5
49	127863.0
50	118867.0
51	109871.0
52	89172.0
53	68473.0
54	62564.5
55	56656.0
56	52736.5
57	48817.0
58	45847.5
59	42878.0
60	37438.0
61	31998.0
62	27466.5
63	22935.0
64	18539.0
65	14143.0
66	11072.0
67	8001.0
68	6337.5
69	4674.0
70	3776.5
71	2879.0
72	2307.0
73	1735.0
74	1368.5
75	849.0
76	696.0
77	508.0
78	320.0
79	237.0
80	154.0
81	85.5
82	17.0
83	16.0
84	15.0
85	8.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1014591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.269328502451796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60661885378012	21.54416307186052
2	10.853312212551314	6.353383209776727
3	4.0188958440942555	3.5289114803379915
4	1.9954008734092907	2.3361617463158306
5	1.2911313361906362	1.889527360938663
6	0.8947402725388292	1.5713068176787346
7	0.718251409768238	1.471591552189897
8	0.649084284611775	1.5198608921664767
9	0.5573775364614074	1.4682659593118612
>10	5.209183578795374	26.1744011545183
>50	0.11615472029489451	2.3543410968634975
>100	0.07481729267434337	4.307208736191489
>500	0.007174260941374847	1.4109045912399178
>1k	0.004782840627583232	3.663752248265811
>5k	6.832629467976045E-4	1.6397891135183145
>10k+	0.002391420313791616	18.766430968825944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	55308	5.451260655771636	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	45292	4.464064830064529	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	34942	3.4439493352493766	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATAACCCT	16527	1.6289322495468617	No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	14323	1.4117018581871907	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTA	10862	1.0705791791963462	No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	10423	1.0273105123148145	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC	8638	0.8513775501655347	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT	7761	0.7649387782860286	No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT	4569	0.4503292459720222	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	3867	0.38113880371499453	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATAACCCTCGT	3808	0.37532365258513034	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3497	0.3446709067988973	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATG	3366	0.33175930005292775	No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	3131	0.30859725741702815	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTAT	2447	0.2411809290640268	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT	2088	0.20579721286705677	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCATAACCC	2035	0.20057343303853475	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2009	0.19801082406605222	No Hit
TGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCTT	1780	0.17544015273149477	TruSeq Adapter, Index 13 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1626	0.16026162266371374	No Hit
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	1408	0.13877513204828348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5174498886743525	0.0
2	0.0	0.0	0.0	2.931033293218647	0.0
3	0.0	0.0	0.0	3.5759236973322257	0.0
4	0.0	0.0	0.0	5.491276780495786	0.0
5	0.0	0.0	0.0	10.523846554917203	0.0
6	0.0	0.0	0.0	11.41188912576595	0.0
7	0.0	0.0	0.0	12.969561133501086	0.0
8	0.0	0.0	0.0	14.857908260570023	0.0
9	0.0	0.0	0.0	15.37486533982659	0.0
10	0.0	0.0	0.0	21.12141739873506	0.0
11	0.0	0.0	0.0	22.946192110909717	0.0
12	0.0	0.0	0.0	30.347401070973426	0.0
13	0.0	0.0	0.0	31.188626747132588	0.0
14	0.0	0.0	0.0	31.644968268001588	0.0
15	0.0	0.0	0.0	33.13699806128775	0.0
16	0.0	0.0	0.0	34.01774705275328	0.0
17	0.0	0.0	0.0	34.867153365247674	0.0
18	0.0	0.0	0.0	35.533136012442455	0.0
19	0.0	0.0	0.0	38.09052120509644	0.0
20	0.0	0.0	0.0	38.9998531427935	0.0
21	0.0	0.0	0.0	39.851822064260375	0.0
22	0.0	0.0	0.0	40.848578392672515	0.0
23	0.0	0.0	0.0	41.62120499787599	0.0
24	0.0	0.0	0.0	42.251803928873805	0.0
25	0.0	0.0	0.0	42.82977081405217	0.0
26	0.0	0.0	0.0	43.35628839601376	0.0
27	9.856188355701953E-5	0.0	0.0	44.02039836742096	0.0
28	1.9712376711403907E-4	0.0	0.0	44.56248872698457	0.0
29	1.9712376711403907E-4	0.0	0.0	45.21063167325553	0.0
30	1.9712376711403907E-4	0.0	0.0	45.96689700578854	0.0
31	1.9712376711403907E-4	0.0	0.0	46.500412481482684	0.0
32	2.956856506710586E-4	0.0	0.0	47.02062210289664	0.0
33	2.956856506710586E-4	0.0	0.0	47.587155809582384	0.0
34	2.956856506710586E-4	0.0	0.0	48.189368918115775	0.0
35	2.956856506710586E-4	0.0	0.0	48.827261428496804	0.0
36	2.956856506710586E-4	0.0	0.0	49.343824260219144	0.0
37	2.956856506710586E-4	0.0	0.0	49.91163927139113	0.0
38	2.956856506710586E-4	0.0	0.0	50.67943634430031	0.0
39	2.956856506710586E-4	0.0	0.0	52.22932196323445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	20	7.0328964E-4	45.0	25
CGCGAAC	20	7.0328964E-4	45.0	32
CCCCGTT	20	7.0328964E-4	45.0	16
TGCGATC	20	7.0328964E-4	45.0	32
ATTTCGG	25	3.8904815E-5	45.0	2
TACCGGG	70	0.0	45.0	3
GATACGC	60	0.0	44.999996	5
GCGCGAC	30	2.1652486E-6	44.999996	9
TACGGCT	6590	0.0	44.009865	7
ACCGGTG	195	0.0	43.846153	17
ACGGCTG	6635	0.0	43.677467	8
GATACCT	4730	0.0	43.477802	5
TGATACC	4755	0.0	43.059933	4
GGCGACT	1230	0.0	42.80488	10
CGGCTGT	6915	0.0	42.624733	9
AGGCACG	90	0.0	42.5	10
GCGACTG	1230	0.0	42.256096	11
CGTTTGG	560	0.0	42.1875	2
CGTTTTT	1910	0.0	41.937172	1
AAGGCGA	70	0.0	41.785713	30
>>END_MODULE