SRR2933840.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933840.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287897
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	25765	8.949381202304991	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	22509	7.818421171460627	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	16730	5.811106055290607	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT	8406	2.919794231964904	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	6390	2.2195437951767474	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA	4993	1.7343008089698746	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	4668	1.6214132137535298	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC	4070	1.4137000385554555	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	3468	1.2045974775701032	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT	1835	0.6373807299138233	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	1830	0.6356439976797257	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG	1629	0.5658273618690017	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1404	0.48767441133460926	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1305	0.45328711309947656	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	1142	0.3966696422678944	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGCAGTA	957	0.3324105496062828	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	932	0.3237268884357947	No Hit
TGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCTT	642	0.2229964188581333	TruSeq Adapter, Index 15 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.2073658287512548	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG	512	0.1778413807715954	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCAGCAGTATCG	485	0.1684630267074683	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	454	0.15769528685606313	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	408	0.14171735030236507	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGCAGTATCGTATGCCGTCTTC	380	0.13199164979141847	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	378	0.1312969568977794	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCGTATCGTATGCCGTCTTCTGC	366	0.12712879953594514	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGT	330	0.11462432745044235	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	316	0.10976147719496904	No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG	305	0.10594066627995428	No Hit
GACTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	302	0.10489862693949573	No Hit
GAATCTTTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	298	0.10350924115221764	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGACA	35	1.2076816E-7	45.000004	15
TGCGGGT	35	1.2076816E-7	45.000004	4
GGGACCG	70	0.0	45.000004	7
TTGCGCG	35	1.2076816E-7	45.000004	1
TTGATCC	20	7.024468E-4	45.0	23
ATGGGCG	20	7.024468E-4	45.0	5
GGAATAT	20	7.024468E-4	45.0	8
CAAACGG	25	3.883498E-5	45.0	27
ACACGAG	20	7.024468E-4	45.0	35
GTTCTCG	25	3.883498E-5	45.0	1
TTGTGCG	25	3.883498E-5	45.0	1
CCGATTC	25	3.883498E-5	45.0	11
TCTCTCG	25	3.883498E-5	45.0	1
CGCGGGT	25	3.883498E-5	45.0	4
CGACGGA	20	7.024468E-4	45.0	41
ATGGCAC	20	7.024468E-4	45.0	25
ACCATCG	50	2.1827873E-11	45.0	40
TTTGTCG	20	7.024468E-4	45.0	1
TATGGCA	20	7.024468E-4	45.0	24
GTGGGCG	25	3.883498E-5	45.0	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content