SRR2933839.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933839.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742570
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	38503	5.185100394575596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	34824	4.689658887377621	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	29117	3.921111814374403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	14932	2.010854195564055	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	14565	1.961431245539141	TruSeq Adapter, Index 15 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	8647	1.1644693429575663	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	8264	1.112891713912493	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	8123	1.0939036050473356	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	8070	1.0867662307930566	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	7723	1.0400366295433427	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	7490	1.008659116312267	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	7097	0.9557348128795937	TruSeq Adapter, Index 14 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	5194	0.6994626769193477	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	4226	0.5691045961996849	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	3794	0.5109282626553725	TruSeq Adapter, Index 15 (95% over 22bp)
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	3604	0.48534144929097595	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTGT	3172	0.42716511574666366	No Hit
TGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCTT	2968	0.39969295823962725	TruSeq Adapter, Index 15 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	2631	0.3543100313775132	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	2351	0.3166031485247182	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.31256312536191877	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	2286	0.30784976500531935	No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	2071	0.27889626567192316	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	2012	0.2709508867850842	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	1964	0.2644868497246051	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	1905	0.25654147083776613	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	1674	0.22543329248421026	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	1635	0.22018126237257096	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCGACTCGTATGCCGTCTTCTGC	1608	0.21654524152605142	TruSeq Adapter, Index 14 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	1363	0.1835517190298558	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	1296	0.17452900063293697	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	1175	0.1582342405429791	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	1092	0.1470568431259006	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	989	0.1331860969336224	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	941	0.12672205987314328	No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGT	922	0.12416337853670362	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	906	0.1220086995165439	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	904	0.12173936463902393	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCGACTCGTATGCCGTCTTCTGC	879	0.11837267867002438	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGTGAGACTCG	822	0.11069663466070538	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	810	0.1090806253955856	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCTGTGAGAC	788	0.106117941742866	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	766	0.10315525809014639	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	747	0.10059657675370673	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	35	1.2111559E-7	45.000004	1
ACCGGTC	35	1.2111559E-7	45.000004	17
ATAGGTA	20	7.0316705E-4	45.000004	33
TCTTACG	35	1.2111559E-7	45.000004	1
TTCCGCG	20	7.0316705E-4	45.000004	1
ATCGACG	20	7.0316705E-4	45.000004	16
TAGCGGG	65	0.0	44.999996	3
CTCAACT	3495	0.0	44.4206	2
GCTCAAC	3685	0.0	44.267296	1
TTGACCC	3170	0.0	44.148266	8
CTTGACC	3630	0.0	44.070248	7
TACGGCT	4140	0.0	44.02174	7
ACCCTGC	2735	0.0	44.0128	11
CTGCATT	2665	0.0	43.986866	14
TGCATTA	2650	0.0	43.896225	15
ACTTGAC	3345	0.0	43.856506	6
TCAACTT	3420	0.0	43.81579	3
CACCGGT	180	0.0	43.75	16
GCATTAG	2655	0.0	43.728813	16
CCTGCAT	2760	0.0	43.695652	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content